INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Bin Li,Xiaojuan Li,Shuying Li,Chen Chen,Ting Xiao,Yuehong Xu
Psoriasis is a chronic and incurable inflammatory skin disease usually requiring long-term disease management. Natural phytochemical resveratrol (RES) has been known for high efficiency and low toxicity, exhibiting good anti-psoriasis potential. However, its biological activity is limited by poor solubility, chemical instability, and insufficient skin retention. Herein, we developed an adhesive eutectogel loaded with RES constructed through electrostatic and hydrogen bonding cross-linking between cyclodextrin-levulinic acid supramolecular deep eutectic solvent (DES) and chitosan (CS). A series of characterizations combined with molecular dynamics simulations demonstrated that such DES not only significantly increased the solubility and stability of RES, but also acted as the cross-linker and plasticizer in eutectogel preparation. When applied to the skin surface, the eutectogel formed a soft film in situ that established tight contact with the skin, thereby enhancing RES sustained penetration into the skin. In an imiquimod-induced psoriasis-like mice model, the eutectogel effectively alleviated psoriasis symptoms, inhibited the excessive proliferation of keratinocytes, and regulated the expression of IL-23/IL-17 axis-related factors, whose therapeutic effect was better than that of commercially available calcipotriol ointment. Overall, The DES-strengthened eutectogel offers a promising topical drug delivery platform for psoriasis treatment and expands the applications of green polysaccharide medical materials.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Suping Ji,Wenjuan Wang,Yulin Huang,Qiang Xia
Mucoadhesive films based on tamarind seed polysaccharide and guar gum (TSP-GG) were formulated for buccal delivery of resveratrol. Resveratrol-bovine serum albumin nanoparticles (Res-BSA) were prepared and dispersed in TSP-GG to improve its buccal mucoadhesiveness. The impregnation of Res-BSA induced the dense internal structures of TSP-GG and improved its strength and rigidity. Structural characterization showed that resveratrol existed in an amorphous state in the films containing Res-BSA, and hydrogen bonding was formed between Res-BSA and the film matrices. The films containing Res-BSA exhibited good uniformity in thickness, weight, and resveratrol content, and their surface pH was near neutral, ranging between 6.78 and 7.09. Increasing Res-BSA content reduced the water contact angle of TSP-GG (from 75.9° to 59.6°). The swelling and erosion studies indicated the favorable hydration capacity and erosion resistance of the films containing Res-BSA. Additionally, the addition of Res-BSA imparted enhanced ex vivo mucoadhesive force, in the range of 1.53 N to 1.98 N, and extended ex vivo residence time, between 17.9 h and 18.9 h, to TSP-GG. The current study implied that the composite systems of TSP-GG and Res-BSA may be a novel platform for buccal mucosal delivery of resveratrol.
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Food Bioscience
·
2025
·
IF 5.9
Yun-Lu Song,Jia-Lin Xing,Charles Ampomah-Dwamena,Shuai-Hui Ma,Mao-Song Pei,Han-Li Liu,Rui-Ping Feng,Yong-Ke Wu,Tong-Lu Wei,Hai-Nan Liu,Da-Long Guo
Grape seed extract (GSE) is a natural polyphenolic compound with potent antioxidant activity. We developed a composite preservation coating material by combining GSE with nano-zinc oxide (Nano-ZnO) and chitosan (CHI), two widely used agents in postharvest preservation. The CHI-GSE-Nano-ZnO coating effectively inhibited postharvest decay and berry abscission while delaying quality deterioration in grapes. Treatment with the composite coating enhanced the activities of antioxidant enzymes (SOD, POD, CAT, and APX) while reducing oxidative stress markers (O 2 - , H 2 O 2 , and malondialdehyde), thereby mitigating ROS accumulation to preserve postharvest grape quality. To elucidate the underlying mechanism, transcriptome sequencing was conducted on ‘Kyoho’ grape samples collected 3, 6, and 9 days post-treatment with CHI, CHI-GSE, and CHI-GSE-Nano-ZnO. Transcriptomic profiling revealed upregulation of VvSTS484 , a pivotal stilbene synthase (STS)-encoding gene, in the CHI-GSE-Nano-ZnO group. Promoter analysis identified multiple MYB-binding cis-elements in VvSTS484 . Yeast one-hybrid and transient dual-luciferase assays demonstrated that the transcription factor VvMYB714 directly binds to four specific MBS motifs in the VvSTS484 promoter (MBS1, -410 to -416 bp; MBS2, -1201 to -1207 bp; MBS3, -1554 to-1560 bp; and MBS4, -1906 to -1912 bp). This molecular interaction activated VvSTS484 expression, leading to enhanced resveratrol biosynthesis that peaked at day 9 post-treatment. The elevated resveratrol level in the CHI-GSE-Nano-ZnO group aligned with reduced oxidative stress and delayed quality deterioration, consistent with its established role in enhancing plant stress tolerance. In conclusion, the CH-GSE-Nano-ZnO composite coating inhibits postharvest decay via the MYB- pSTS module, providing a theoretical basis for developing natural product-based preservation technologies for fruit and vegetables.
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DRUG DELIVERY
·
2022
·
IF 6.819
Wanmei Wang,Yan Liu,Pan Pan,Yueqi Huang,Ting Chen,Tianyu Yuan,Yulong Ma,Guang Han,Jiahuan Li,Yiguang Jin,Fei Xie
Smoke bombs are often used in military/fire training, which can produce a large amount of zinc chloride (ZnCl2) smoke. Inhalation of ZnCl2 smoke usually causes acute lung injury (ALI) that would likely develop to acute respiratory distress syndrome (ARDS). However, there is no effective prevention or treatment strategy for the smoke-induced ALI. Resveratrol (RES) is a natural polyphenol with good anti-inflammatory and anti-apoptotic activities, but its low solubility, stability, and bioavailability restrict its clinical application. In this study, an inhalable RES formulation composed of RES-β-cyclodextrin inclusion complexes (RES-β-CD) was prepared for the prevention of ZnCl2 smoke-induced ALI. RES-β-CD powders had a small mass median aerodynamic diameter of 3.61 μm and a high fine particle fraction of 38.84%, suitable for pulmonary inhalation. RES-β-CD exhibited low BEAS-2B cytotoxicity. Pulmonary delivery of RES-β-CD to mice remarkably prevented the smoke-induced ALI with downregulation of TNF-α, IL-1β, STAT3, and GATA3, and upregulation of T-bet and Foxp3. RES-β-CD protected the respiratory function, percutaneous oxygen saturation, physical activity, lung capillary integrity, and lung liquid balance, alleviating inflammation and apoptosis. Pulmonary delivery of the positive drug, budesonide (BUD), also alleviated the smoke-induced ALI by reduction of inflammation and cell apoptosis. RES-β-CD exhibited the regulation of the Th1/Th2 and Treg/Th17 balances, while BUD did not show any effect on immune balances. In conclusion, pulmonary delivery of RES-β-CD is a promising anti-inflammatory and anti-apoptosis strategy for the prevention of ZnCl2 smoke-induced ALI by direct lung drug distribution and regulation of immune balance.
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ACTA HISTOCHEMICA
·
2021
·
IF 2.479
Dongmin Yu,Jianxian Xiong,Yizhou Gao,Jinghang Li,Dawei Zhu,Xiaowei Shen,Lifu Sun,Xiaowei Wang
Resveratrol is a kind of iPolyphenols widely existing in herbal medicine. Here we aim to investigate whether resveratrol can reduce the degree of myocardial ischemia/reperfusion (IR) injury and inhibit the development of oxidative stress, and elucidate the molecular mechanism of resveratrol in protecting myocardial cells. The primary rat cardiomyocytes were used to establish an ischemia/reperfusion model in vitro, and a series of routine biochemical experiments were conducted to explore the antioxidant and anti-apoptotic effects of resveratrol in myocardial ischemia-reperfusion injury. Compared with that of the simulated ischemia-refusion (SIR) group, cell viability in the SIR and resveratrol co-treatment groups increased significantly (P < 0.001), the release of lactate dehydrogenase (LDH) and creatine kinase MB (CK MB) decreased, the positive rate of reactive oxygen species (ROS) in cardiomyocytes decreased, and the concentration of catalase and glutathione peroxidase increased significantly (P < 0.001). Besides, resveratrol can activate PI3K/AKT signaling pathway. PI3K siRNA can inhibit the PI3K/AKT signaling mediated by resveratrol. The addition of resveratrol can significantly increase the activity of mitochondrial superoxide dismutase (SOD) and reduce the malondialdehyde (MDA), which indicates that the oxidative damage of mitochondria induced by resveratrol was significantly weakened. The mitochondrial functional changes induced by resveratrol can be reversed by PI3K siRNA. In conclusion, our study shows that resveratrol can reduce ROS in cardiomyocytes by PI3K/AKT signaling pathway activation, and effectively inhibit the apoptosis of cardiomyocytes, thus having a direct protective effect on cardiomyocytes under SR.
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TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2025
·
IF 3.4
Sangping Li,Shule Tan,Xiangsheng Tian,Yiquan Ou,Shujuan Hu,Weichao Zhao,Dingxin Long
This study investigated the protective effects of resveratrol (Res) against tri-o-cresyl phosphate (TOCP)-induced spinal cord neurotoxicity in adult hens, focusing on its modulation of ferroptosis via the p62/Keap1/Nrf2 pathway. Resveratrol is a classical neuroprotective compound with antioxidant properties and the ability to activate Nrf2. Adult hens were assigned to six groups: Control, TOCP, Ferrostatin-1 (Fer-1), Ferrostatin-1 + TOCP, Resveratrol, and Resveratrol + TOCP. Spinal cord tissues were analyzed using behavioral OPIDN scoring, histology (hematoxylin–eosin and Nissl staining), biochemical assays, and Western blotting for ferroptosis- and p62/Keap1/Nrf2-related proteins. TOCP exposure induced severe ultrastructural damage, including myelin sheath disruption and neuronal degeneration, along with increased malondialdehyde (MDA) and Fe 2+ levels and decreased glutathione (GSH) and superoxide dismutase (SOD) activity. Western blot analysis demonstrated upregulation of NCOA4, ACSL4, Nrf2, P62, and LC3 II, with downregulation of GPX4, SLC7A11, FTH1, and Keap1. Resveratrol treatment significantly attenuated these molecular, biochemical, and histopathological alterations, mitigating oxidative stress and ferroptotic changes.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Jianan Liu,Jinle Ma,Siyang Pei,Siyi Bao,Hongshun Hao,Jingran Bi,Hongman Hou,Gongliang Zhang
Staphylococcus aureus ( S. aureus ) is a typical foodborne pathogen, and the development of novel synergistic antimicrobial strategies is urgently needed. Therefore, the aim of this study was to investigate the mechanism underlying the synergistic inhibition of S. aureus by benzyl isothiocyanate (BITC) and resveratrol (RES). The synergistic effect of BITC and RES on S. aureus was verified by a time-elimination curve, cell hemolysis, intracellular reactive oxygen species accumulation and transmission electron microscopy. The results of the transcriptomic and proteomic analyses revealed that the combination of BITC and RES against S. aureus synergistically downregulated the expression of the cell wall anchoring protein immunoglobulin G-binding protein (Sbi) compared with that in the individual treatments, a finding further supported by qRT-PCR and molecular docking. In addition, synergistic effects significantly disrupted cell wall integrity, alkaline phosphatase leakage increased by 1.1–1.9 times, the zeta potential increased by 3.0–6.3 mV, and the hydrophobicity decreased by 19.2 %–25.0 %. These findings uncover a novel mechanism of synergistic antimicrobial action and provide valuable clues for developing strategies against S. aureus .
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Food & Function
·
2025
·
IF 5.4
Junzhu Li,Xin Feng,Yizhi Jing,Haidong Qian,Yun Ji,Yanling Hao,Zhengyuan Zhai
Infectious enteritis is a chronic inflammatory condition of the intestine, mainly characterized by immune cell infiltration and gut microbiota dysbiosis. Resveratrol, a natural polyphenol compound from grapes, berries and peanuts, is known for its antioxidant and anti-inflammatory properties. This study investigated the effects of resveratrol in a murine model of Citrobacter rodentium-induced colitis. Resveratrol ameliorated disease symptoms, body weight loss, colon shortening, and increased intestinal permeability. Histological and immunofluorescence analyses revealed that resveratrol improved intestinal barrier integrity by increasing goblet cells, upregulating MUC-2 expression, reducing crypt hyperplasia, and suppressing epithelial cell apoptosis. Resveratrol significantly decreased the mRNA levels of pro-inflammatory cytokines, namely Tumor Necrosis Factor-α (TNF-α), Interleukin-6 (IL-6) and Interleukin-22 (IL-22), while restoring the percentage of CD4+ cells to normal levels. It also attenuated oxidative stress by enhancing the activities of key antioxidant enzymes, including catalase (CAT), total superoxide dismutase (T-SOD), and glutathione peroxidase (GSH-Px). Furthermore, resveratrol reshaped the gut microbiota by increasing the abundance of obligate anaerobes Clostridium and decreasing the abundance of facultative anaerobes Enterobacteriaceae. The abundance of Lactobacillus and Dubosiella was also significantly increased by resveratrol. In summary, these findings demonstrate that resveratrol supplementation may serve as a promising nutritional strategy to ameliorate C. rodentium colitis by inhibiting CD4+ cell activation and remodeling the gut microbiota.
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Journal of Neuroimmune Pharmacology
·
2026
·Vol. 21
·期 1
·33
·
IF 6.1
Zhang Chu,Ma Yunhan,Shi Zhidan,He Xinqi,Hu Jiayi,Chen Shuting,Wu Hao,Tian Meng,Yan Chuang,Xing Mengtao,He Ling
Aging is a major risk factor for neurodegenerative diseases, including Alzheimer’s disease (AD). Targeting cellular senescence has therefore emerged as a promising therapeutic strategy. Resveratrol (RV), a natural polyphenolic compound, exhibits anti-aging properties through the regulation of autophagy and oxidative stress; however, its mechanisms in AD remain incompletely understood. In this study, we investigated the effects and underlying mechanisms of RV in an Aβ1–42-induced AD model. In vivo, RV administration significantly reduced the expression of aging-related markers and activated autophagy-associated signaling pathways. In vitro, RV treatment markedly attenuated Aβ1–42-induced cell viability loss and excessive reactive oxygen species (ROS) production. Further mechanistic analyses demonstrated that RV-induced autophagy activation was closely associated with the AMP-activated protein kinase/UNC-51-like kinase 1 (AMPK/ULK1) and silent information regulator 1/nuclear factor-kappaB (SIRT1/NF-κB) pathways. Collectively, these findings suggest that RV alleviates AD-related pathological processes by promoting autophagy and delaying cellular senescence, highlighting its potential as a therapeutic agent for age-related neurodegenerative diseases. Graphical
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NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
·
2026
·
IF 4
Fu Xingqian,Liu Wei,Zhang Yongxiang,Wu Qihong,Long Gang,Zhai Yifa,Song Chao,Yang Po
The pathophysiology of osteoarthritis (OA), a common degenerative joint disease, is complicated. The significance of autophagy irregularities and iron metabolism issues in OA has garnered a lot of interest in recent years, with “Ferritinophagy” mediated by NCOA4 acting as a crucial link between the two and potentially a novel therapeutic target. The purpose of this research is to determine whether resveratrol, a naturally occurring substance, protects cartilage via controlling the ferritinophagy network. Using differential analysis, weighted gene co-expression network analysis, and other machine learning methods, this study combined transcriptome data from several omics studies and screened OA core ferritinophagy-related genes. Use molecular docking and molecular dynamics simulations to assess resveratrol’s binding mechanism and stability to core targets. The CCK-8 technique was employed in vitro to assess cell survival and identify the ideal resveratrol intervention concentration in an inflammatory model of human articular chondrocytes caused by IL-1β. Using Western blot (WB) to determine the expression of 8 key proteins including NCOA4, GPX4, and LC3. Transmission electron microscopy is used to observe the mitochondrial ultrastructure. NCOA4 protein location and expression were verified using immunofluorescence. Bioinformatics analysis successfully screened key genes such as DDIT4, CTSB, BNIP3, CDKN1A, and NCOA4. Molecular docking and molecular dynamics simulations demonstrate that resveratrol can stably bind to core proteins such as BNIP3 and NCOA4. Resveratrol has been shown in vitro to dramatically increase cell survival and reverse the aberrant expression of important ferritinophagy proteins brought on by IL-1β, upregulating GPX4 and CDKN1A and downregulating NCOA4, LC3-II/I, and BNIP3. The results of transmission electron microscopy demonstrated that resveratrol successfully reduced aberrant autophagosome aggregation and mitochondrial damage. Resveratrol can considerably reduce the unusually high expression of NCOA4, as further demonstrated by immunofluorescence. This study reveals the molecular mechanism by which resveratrol inhibits NCOA4-mediated ferroptophagy, thereby regulating downstream autophagy and ferroptosis processes, and alleviating chondrocyte damage in OA. This provides a new perspective for elucidating the cartilage protective effect of resveratrol and lays a solid theoretical and experimental foundation for developing OA treatment strategies targeting ferritinophagy.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2026
·
IF 5.2
Cuilian Wei,Yusong Pan,Qianqian Liu,Ling Peng,Run Huang,Rui Yue,Xiuling Lin,Jun Tao
Secondary injury after spinal cord injury (SCI) is characterized by persistent oxidative stress and neuroinflammation, which create a hostile microenvironment that limits neuronal regeneration. Herein, an interfacially engineered core-shell nanozyme system (CeO 2 @mSiO 2 -RES) was developed for integrated regulation of the oxidative-inflammatory microenvironment. The CeO 2 core exhibited mixed Ce 3+ /Ce 4+ states and CAT- and SOD-like activities, while the mesoporous SiO 2 shell enabled efficient RES loading and pH-dependent release. In vitro, CeO 2 @mSiO 2 -RES reduced intracellular ROS accumulation and restored CAT, SOD, and GSH antioxidant defenses. It also decreased iNOS expression while increasing Arg-1 expression in LPS-stimulated macrophages. Furthermore, CeO 2 @mSiO 2 -RES enhanced NGF-induced neurite outgrowth and MAP2 expression under inflammatory stress. Collectively, these findings suggest that CeO 2 @mSiO 2 -RES can regulate the oxidative-inflammatory microenvironment and provide a potential nanomaterial-based strategy for neuronal repair after SCI. Download: Download high-res image (383KB) Download: Download full-size image
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Yunze Ma,Jian Li,Rui Zhao,Huajiang Zhang,Zhanhui Geng,Hanyu Li,Yuepeng Wang,Ning Xia,Lina Xu
pH-dependent covalent conjugation of egg white protein and resveratrol was studied. • Alkaline treatment boosted grafting efficiency and antioxidant activity. • A double-layer coating with active-barrier synergy was developed. • The coating effectively extended the shelf life of nuts during storage. High-fat nuts are highly susceptible to quality deterioration due to oxidative rancidity during storage. Existing single-function edible film coatings often struggle to simultaneously achieve efficient antioxidant activity and high barrier properties, limiting their practical effectiveness in nut preservation. This study developed a double-layer active coating that integrates active antioxidant capability with efficient physical barrier functionality. A pH-shift alkaline treatment method was employed to covalently bind the natural antioxidant Resveratrol (RES) to Egg White Protein (EWP), forming an active composite that served as the functional inner layer. Structural characterization of the covalent complex revealed that pH 9 treatment promoted the formation of a stable covalent complex between RES and EWP, increasing its DPPH radical scavenging rate by 39.8% compared to untreated EWP. Meanwhile, a blended system of zein and xanthan gum constituted a high-barrier outer layer, resulting in the double-layer coating—DL. The DL coating exhibited outstanding comprehensive performance, with water vapor transmission rate and oxygen permeability reaching 47.6% and 46.7% of those of the single-layer ZEIN coating, respectively. Its DSC profile also demonstrated improved thermal stability. In preservation studies on cashews and almonds, after 180 days of storage, the peroxide value (POV) and acid value (AV) of the DL coating treatment group were lower than those of other single-layer coating groups and the blank control group, effectively retarding the lipid oxidation process. This study confirms that optimizing the antioxidant activity of the active layer through pH-modulated covalent binding, combined with a double-layer structural design to achieve functional synergy, can significantly enhance the overall preservation performance of protein-based coatings. This strategy provides a new approach with both theoretical foundation and process feasibility for developing green packaging materials specifically designed for high-fat nuts. Download: Download high-res image (214KB) Download: Download full-size image
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危险品化学品经营许可证（带存储）