International Journal of Peptide Research and Therapeutics
·
2021
·Vol. 27
·期 2
·1343-1350
·
IF 1.931
Zhao Yi,Sun Guangming,Yuan Libo,Li Jinlei,Lu Kui
Breast cancer susceptibility gene 1 (BRCA1) is an important tumor suppressor gene in the human which associated with a variety of tumor suppressor genes to inhibit the growth of malignant tumors. Breast cancer is closely related to BRCA1 mutations in humans. Four BRCA1 (856-871) mutants were screened that can interact well with RAD51 (158-180) using computer to simulate mutations at Y 856, R 866 and Q 867. These mutants were synthesized by using Fmoc solid-phase synthesis, and purified by liquid chromatography. According to the results of fluorescence spectroscopy, R866W has the strongest binding ability for the above peptides and RAD51 (158-180). Based on the circular dichroism spectrum results, the sequence of the interaction ability of the above peptides on the secondary structure of RAD51 (158-180) is Y856P, Q867C > Y856P, R866W ≈ R866W > Y856P > BRCA1. All the spectral results show that R866W have the strongest interaction ability with RAD51(158-180).
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 8
·2045-2054
·
IF 4.157
Deng Xunxun,Yang Xinlan,Guan Xingxing,Song Jie,Wu Shuo
A photoelectrochemical (PEC) immunosensing strategy based on the multiple quenching of polydopamine nanoparticles (PDA NPs) to Mn 2+ -doped CdS-modified TiO 2 nanoparticles (TiO 2 /CdS:Mn) was designed for the highly sensitive detection of carcinoembryonic antigen (CEA). The uniform PDA NPs possessed good dispersibility, good biocompatibility, and abundant functional groups for biomolecule assembly. They also had unique photophysical properties, with light absorption spanning the visible to infrared light range. When the immune-recognition brought the PDA NPs close to the TiO 2 /CdS:Mn interface, the PDA NPs competed with TiO 2 /CdS:Mn to absorb light, consumed photoelectrons generated in the TiO 2 /CdS:Mn, and hindered the access of electron donors to photoactive materials. The contribution from these aspects thus led to a significant decrease in photocurrent. Benefiting from the multiple quenching mechanism, the PEC immunosensor showed high sensitivity for CEA detection. Under optimal conditions, a low detection limit of 0.02 pg/mL and a wide linear relationship from 0.1 pg/mL to 100 ng/mL were obtained. The immunoassay showed good reproducibility and stability, and good selectivity and high accuracy in serum sample analysis. In this regard, PEC immunosensors may have great application potential for screening tumor markers and the prevention and monitoring of serious diseases.
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Nano-Micro Letters
·
2021
·Vol. 13
·期 1
·1-13
·
IF 16.419
Qian Guangfu,Chen Jinli,Yu Tianqi,Luo Lin,Yin Shibin
N-doped graphene-coated structure and mesoporous nano-sheet can efficiently boost active sites and stability for hydrogen and oxygen evolution reaction.
NiCo@C-NiCoMoO/NF exhibits low overpotentials for HER (266 mV) and OER (390 mV) at ± 1000 mA cm −2 .
For water electrolysis, it can hold at 1000 mA cm −2 for 43 h in 6.0 M KOH + 60 °C condition.
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JOURNAL OF MATERIALS SCIENCE
·
2021
·Vol. 56
·期 15
·9419-9433
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IF 4.22
Xuan Haicheng,Wang Rui,Yang Jing,Zhang Guohong,Liang Xiaohong,Li Yuping,Xie Zhigao,Han Peide
Currently, substantial attention has been concentrated on the preparation and practical application of complex hierarchical nanostructure composite, which exhibits excellent electrochemical properties compared to the single-structured materials. Hence, a novel electrode of NiMoO 4 @Co 3 O 4 –5H composite nanoarrays supported on reduced graphene oxide/Ni Foam (rGO/NF) was synthesized through the facile hydrothermal method. The composite combines the advantages of the large specific capacitance of NiMoO 4 and the great rate capability of Co 3 O 4 , exhibiting the distinguished specific capacitance and rate performance. The prepared NiMoO 4 @Co 3 O 4 –5H composite shows an enhanced pseudocapacitive performance of about 1722.3 F g −1 at a current density of 1 A g −1 , and eminent rate capability of 80.8% at 10 A g −1 . Moreover, the composite delivers good long-term cycling stability with capacitance retention of 91% after 6000 cycles. An asymmetric supercapacitor (ASC) was fabricated using NiMoO 4 @Co 3 O 4 –5H and AC as the positive electrode and negative electrode, achieving the high energy density of 37.1 Wh kg −1 at a power density of 798.0 W kg −1 , and exceptional cycling stability (100% retention after 4000 cycles). These consequences suggest that NiMoO 4 @Co 3 O 4 –5H could be considered as a potential electrode material for energy storage devices. Graphical abstract
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Scientific Reports
·
2021
·Vol. 11
·期 1
·1-15
·
IF 4.38
Dosoky Waleed M.,Fouda Moustafa M. G.,Alwan Ali B.,Abdelsalam Nader R.,Taha Ayman E.,Ghareeb Rehab Y.,El-Aassar M. R.,Khafaga Asmaa F.
Silver nanoparticles (AgNPs) have been used as a promising alternative to antibiotics in poultry feed. In this study, silver-doped silica nanoparticles (SiO 2 @AgNPs) were prepared in powder form, using starch, via the chemical reduction method and sol–gel technique followed by full characterization. SiO 2 @AgNPs were added to the poultry diet at three doses (2, 4, and 8 mg/kg diet). The safety of the oral dietary supplementation was estimated through the evaluation of the growth performance and hematological, biochemical, and oxidative parameters of birds. Moreover, the immunohistochemical examination of all body organs was also performed. Results of this study showed that SiO 2 @AgNPs have no negative effects on the growth performance and hematological, biochemical, and oxidative parameters of birds. Moreover, the immunohistochemical examination revealed the minimum inflammatory reactions and lymphoid depletion under a dose level of 8 mg/kg. In conclusion, SiO 2 @AgNPs could be considered as a promising and safe nano-growth promoter in broilers when added to poultry diet under a dose level of 4 mg/kg diet.
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ACTA PHARMACOLOGICA SINICA
·
2021
·Vol. 43
·期 1
·96-110
·
IF 6.15
Liu Xue-qi,Jiang Ling,Li Yuan-yuan,Huang Yue-bo,Hu Xue-ru,Zhu Wei,Wang Xian,Wu Yong-gui,Meng Xiao-ming,Qi Xiang-ming
Diabetic kidney disease (DKD) is one of the microvascular complications of diabetes mellitus and a major cause of end-stage renal disease with limited treatment options. Wogonin is a flavonoid derived from the root of Scutellaria baicalensis Georgi, which has shown a potent renoprotective effect. But the mechanisms of action in DKD are not fully elucidated. In this study, we investigated the effects of wogonin on glomerular podocytes in DKD using mouse podocyte clone 5 (MPC5) cells and diabetic mice model. MPC5 cells were treated with high glucose (30 mM). We showed that wogonin (4, 8, 16 μM) dose-dependently alleviated high glucose (HG)-induced MPC5 cell damage, accompanied by increased expression of WT-1, nephrin, and podocin proteins, and decreased expression of TNF-α, MCP-1, IL-1β as well as phosphorylated p65. Furthermore, wogonin treatment significantly inhibited HG-induced apoptosis in MPC5 cells. Wogonin reversed HG-suppressed autophagy in MPC5 cells, evidenced by increased ATG7, LC3-II, and Beclin-1 protein, and decreased p62 protein. We demonstrated that wogonin directly bound to Bcl-2 in MPC5 cells. In HG-treated MPC5 cells, knockdown of Bcl-2 abolished the beneficial effects of wogonin, whereas overexpression of Bcl-2 mimicked the protective effects of wogonin. Interestingly, we found that the expression of Bcl-2 was significantly decreased in biopsy renal tissue of diabetic nephropathy patients. In vivo experiments were conducted in STZ-induced diabetic mice, which were administered wogonin (10, 20, 40 mg · kg −1 · d −1 , i.g.) every other day for 12 weeks. We showed that wogonin administration significantly alleviated albuminuria, histopathological lesions, and p65 NF-κB-mediated renal inflammatory response. Wogonin administration dose-dependently inhibited podocyte apoptosis and promoted podocyte autophagy in STZ-induced diabetic mice. This study for the first time demonstrates a novel action of wogonin in mitigating glomerulopathy and podocytes injury by regulating Bcl-2-mediated crosstalk between autophagy and apoptosis. Wogonin may be a potential therapeutic drug against DKD.
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Advanced Composites and Hybrid Materials
·
2021
·Vol. 4
·期 3
·716-724
·
IF 5.693
Yan Zhaoqian,Sun Zhihao,Li Anran,Liu Hongshou,Guo Zihao,Qian Lei
Traditional Fe-based oxide with poor intrinsic conductivity, severe volume expansion, and structure destruction exhibits the poor cyclic performance for anode materials of lithium ion batteries (LIBs). Heteroatomic doping Fe-based oxide with nanoarchitectures is deemed to settle the above problems effectively. Herein, with sulfur (S) doping, three-dimensional porous flower-like Fe 2 O 3 (denoted as S- Fe 2 O 3 ) prepared via ordinary solvothermal reaction and calcining process was ingeniously designed as anode materials for LIBs. The S doping changed the morphology, improved the electrical conductivity, and provided more active sites for lithium storage. The flower-like S-Fe 2 O 3 made up of plentiful carbon encapsulated nanoparticles not only relieved the volume expansion but also provided the connected conductive network. The as-prepared flower-like S-Fe 2 O 3 electrode delivered a high discharge/charge capacity (1570.8 mAh g −1 at 0.1 A g −1 after 100 cycles) and the excellent long-cycle performance (521.3 mAh g −1 at 2.0 A g −1 after 1000 cycles). S doping and nanoarchitectures engineering in this work provide rational preparation strategies for composites containing transition metal oxides toward energy storage system. Graphical abstract Three-dimensional porous flower-like S-Fe 2 O 3 from ordinary solvothermal reaction and calcining process is ingeniously designed as anode materials for LIBs.
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Scientific Reports
·
2021
·Vol. 11
·期 1
·1-11
·
IF 4.38
Yang Zuoyi,Mao Xingchen,Cui Jiahao,Wang Yujie,Zhang Yaping
This study examines the specific effect of Tween 80 on the conversion of anthracene (ANT) in laccase medium system regarding surfactant chemical changes and mechanism. The conversion rate and degradation products of ANT were investigated in different concentrations of Tween 80 solution. Between Tween 80 concentration 0–40 critical micelle concentrations (CMC), the kinetic parameter-k (h −1 ) and corresponding half-life T 1/2 decreased with increasing concentration. When Tween 80 was above 20 CMC the laccase-medium system converted > 95% of ANT to anthraquinone within 12 h. During the entire enzymatic reaction, the laccase activity in the system increased with increasing Tween 80 concentration. Combined with GC/MS analysis of the product, it was speculated that hydrogens belonging to the ether-oxygen bond and carbon–carbon double bond α-CH of Tween 80, were removed by the laccase-media system, promoting its degradation. Additionally, enhanced activity caused by oxygen free radicals (ROS) such as RO• and ROO•, continuously oxidized Tween 80, which in turn produced free radicals while converting ANT. This study provides new theoretical support toward the application of surfactants in the elimination of polycyclic aromatic hydrocarbons.
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JOURNAL OF CHEMICAL CRYSTALLOGRAPHY
·
2021
·Vol. 52
·期 1
·53-61
·
IF 0.603
Huang Bin
Abstract Understanding of relationship between structure and property of thermally activated delayed fluorescence (TADF) materials is essential for developing efficient TADF materials. To investigate the substituent effect of electron donors on luminescent properties of TADF materials, a series of single crystals based on triphenylamine (TPA)/anthraquinone (AQ) hybrids namely 1-TPA-AQ, 2-TPA-AQ, 1,8-2TPA-AQ, 2,6-2TPA-AQ have been prepared in this work. Interestingly, it is demonstrated that the substituent site of TPA unit has an important impact on the conformation of AQ for the first time. The planarity of the AQ units in these four molecules is in the order 2,6-2TPA-AQ > 2-TPA-AQ ≈ 1-TPA-AQ > 1,8-2TPA-AQ, which is associated with the molecule symmetry. In addition, intermolecular interactions in these crystals are also investigated. In α-substituted AQ derivatives (1-TPA-AQ and 1,8-2TPA-AQ) typical π–π intermolecular interactions may account for their normal TADF but weak emission in solid state. In contrast, β-substituted AQ derivatives (2-TPA-AQ and 2,6-2TPA-AQ) display weak intermolecular interactions, which are beneficial to restricting molecular motions and to suppressing the non-radiative decay, resulting in efficient TADF and aggregation-induced emission properties simultaneously. Graphic Abstract It is demonstrated that the substituent site of triphenylamine unit on anthraquinone (AQ) group has an important impact on the conformation of AQ.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2021
·Vol. 19
·期 1
·1-15
·
IF 10.435
Li Yan,Wang Juan,Qian Dejian,Chen Liang,Mo Xiumei,Wang Lei,Wang Yan,Cui Wenguo
Background Most of the natural extracellular matrix (ECM) is a three-dimensional (3D) network structure of micro/nanofibers for cell adhesion and growth of 3D. Electrospun fibers distinctive mimicked 2D ECM, however, it is impossible to simulate 3D ECM because of longitudinal collapse of continuous micro/nanofibers. Herein, 3D electrospun micro/nano-fibrous sponge was fabricated via electrospinning, homogenization, shaping and thermal crosslinking for 3D tissue regeneration of cells and vascular. Results Fibrous sponge exhibited high porosity, water absorption and compression resilience and no chemical crosslinked agent was used in preparation process. In vitro studies showed that the 3D short fiber sponge provided an oxygen-rich environment for cell growth, which was conducive to the 3D proliferation and growth of HUVECs, stimulated the expression of VEGF, and well promoted the vascularization of HUVECs. In vivo studies showed that the 3D short fiber sponges had a good 3D adhesion to the chronic wound of diabetes in rats. Furthermore, 3D short fibrous sponges were better than 2D micro/nanofiber membranes in promoting the repair of diabetic full-thickness skin defects including wound healing, hair follicle regeneration, angiogenesis, collagen secretion. Conclusion Therefore, electrospun short fibrous sponges are special candidates for mimicking the 3D ECM and promoting 3D regeneration of tissue. Graphic Abstract
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ADVANCES IN ATMOSPHERIC SCIENCES
·
2021
·Vol. 38
·期 7
·1238-1251
·
IF 3.158
Zhang Hailiang,Xu Yongfu,Jia Long,Xu Min
Acetaldehyde is one of the important VOC species of O 3 precursors in the atmospheric environment. The influences of relative humidity (RH) and initial VOC/NO x ratio ( R CN ) on the formation of O 3 are studied in smog chamber experiments, and the MCM v3.3.1 mechanism of acetaldehyde is modified based on the experimental results. In low-RH conditions (RH = 11.6%±1.1%), the O 3 concentration at 6 h increases first and then decreases with the increase of R CN , and the R CN at the inflection point of O 3 concentrations is 3.2. In high-RH experiments (RH = 78.8%±1.0%), variation of the O 3 concentration at 6 h with R CN is similar to that in low-RH experiments, but the R CN at the inflection point is 2.8. RH has no significant effect on the O 3 concentrations under low R CN (< 3), whereas it has a negative effect under high R CN (> 3). Compared with the experimental results, original MCM v3.3.1 greatly underestimates the O 3 concentrations. Addition of both the photolysis process of peroxyacetyl nitrate and the photolysis process of HNO 3 on the reactor surface into the original MCM can reduce the difference between the simulated O 3 concentrations and the experimental results at 6 h from 24%−35% and 17%−49% to 6%−26% and 10%−42% under low- and high-RH conditions, respectively. The maximum incremental reactivity (MIR) of acetaldehyde simulated with the modified MCM is 4.0 ppb ppb −1 without considering the effect of other VOCs.
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ADVANCES IN ATMOSPHERIC SCIENCES
·
2021
·Vol. 38
·期 7
·1252-1263
·
IF 3.158
Wang Yu,Zhao Jie,Liu Huihui,Li Yuan,Dong Wenbo,Wu Yanlin
Iron and oxalic acids are widely distributed in the atmosphere and easily form ferric oxalate complex (Fe(III)-Ox). The tropospheric aqueous-phase could provide a medium to enable the photo-Fenton reaction with Fe(III)-Ox under solar irradiation. Although the photolysis mechanisms of Fe(III)-Ox have been investigated extensively, information about the oxidation of volatile organic compounds (VOC), specifically the potential for Secondary Organic Aerosol (SOA) formation in the Fe(III)-Ox system, is lacking. In this study, a ubiquitous VOC methacrolein (MACR) is chosen as a model VOC, and the oxidation of MACR with Fe(III)-Ox is investigated under typical atmospheric water conditions. The effects of oxalate concentration, Fe(III) concentration, MACR concentration, and pH on the oxidation of MACR are studied in detail. Results show that the oxidation rate of MACR greatly accelerates in the presence of oxalate when compared with only Fe(III). The oxidation rate of MACR also accelerates with increasing concentration of oxalate. The effect of Fe(III) is found to be more complicated. The oxidation rate of MACR first increases and then decreases with increasing Fe(III) concentration. The oxidation rate of MACR increases monotonically with decreasing pH in the common atmospheric water pH range or with decreasing MACR concentration. The production of ferrous and hydrogen peroxide, pH, and aqueous absorbance are monitored throughout the reaction process. The quenching experiments verify that · OH and \(O_2^{\bar .}\) are both responsible for the oxidation of MACR. MACR is found to rapidly oxidize into small organic acids with higher boiling points and oligomers with higher molecular weight, which contributes to the yield of SOA. These results suggest that Fe(III)-Ox plays an important role in atmospheric oxidation.
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