MARINE POLLUTION BULLETIN
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2025
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IF 4.9
Hao-jie Zheng,Xu-feng Wang,Ye-ni Zhang,Chao-ping Hou,Ke Huang
Diazepam (DIAZ), a widely prescribed benzodiazepine for insomnia and anxiety, has been frequently detected in aquatic ecosystems, raising concerns about potential impacts on human and animal health. To investigate its environmental presence, water and sediment samples were collected from 187 aquaculture ponds across the Pearl River Delta, China. The concentrations of DIAZ and its metabolites—nordazepam (NDZ), oxazepam (OXZ), and temazepam (TEM)—were determined using ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS). Detection frequencies in water ranged from 0.535 % to 58.8 %, with concentrations from below detection (nd) to 392 ng·L −1 , whereas in sediments, frequencies ranged from 1.07 % to 35.8 %, with levels from nd to 1.66 μg·kg −1 . Marked spatial variability was evident, with aquaculture ponds in Foshan (FS) exhibiting the highest DIAZ concentration (392 ng·L −1 ), while Zhongshan (ZS) displayed higher detection frequencies but lower concentrations. Fish ponds contained significantly greater cumulative concentrations of DIAZ and its metabolites compared with shrimp ponds. Strong positive correlations were identified between DIAZ in water and DIAZ and NDZ in sediments. Similarly, NDZ in water was strongly correlated with NDZ and DIAZ in sediments, and TEM in water showed significant correlations with DIAZ and NDZ in sediments. Environmental risk assessment revealed that NDZ and OXZ presented low ecological risks in both matrices at all sites, whereas DIAZ and TEM posed medium to high risks at certain water sites, with DIAZ also showing medium risks in some sediments. The study provides an integrated evaluation of occurrence, spatial distribution, and ecological risks of DIAZ and its metabolites in aquaculture systems, offering critical evidence to support food safety measures and strategies for pharmaceutical pollution control in the Pearl River Delta.
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Italian Journal of Animal Science
·
2025
·
IF 2.3
Yao Huang,Mingmei Shen,Weiyun Zhu,Long Pan
This experiment was conducted to determine the effects of polyethylene glycol (PEG) supplementation on the digestion and fermentation characteristics of sorghum nutrients in the in-vitro simulated porcine gastrointestinal tract. In Exp. 1, high-tannin sorghum grain without or with 50 mg/g PEG was digested by porcine pepsin and pancreatin to measure in-vitro digestibility of nutrients. In Exp. 2, the lyophilised porcine ileal digesta from barrows fed the high-tannin sorghum grain without or with 50 mg/g PEG and the undigested residues from Exp. 1 were, individually, incubated with fresh pig caecal digesta as inoculums for 48 h to simulate the porcine hindgut fermentation. PEG reduced the detectable condensed tannin content by 32% (p < .05) and increased in-vitro digestibility of nutrients especially for crude protein(p < .05). The in-vitro fermentability of nutrients and microbial metabolites including the accumulative gas production excluding the first 12 h, total short-chain fatty acid and microbial protein content in the fermented solutions were improved (p < .05). The relative abundance of Lachnospiraceae NK4A136 was both increased by PEG supplementation regardless of unhydrolyzed residues (p < .05) or ileal digesta (p = .06) as fermentation substrates. In conclusion, PEG supplementation not only directly increased the chemical enzymatic digestion of nutrients in the simulated anterior intestine, but also directly promoted the microbial fermentation including microbial diversities and metabolites in the simulated posterior intestine of porcine. It implies that the increased abundances of Lachnospiraceae by PEG in the hindgut may improve the fermentation capacity of microbiota and thus increase the nutrient digestion in the hindgut.
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Journal of Materials Chemistry C
·
2025
·
IF 5.1
Rui Ding,Wenjuan Zhang,Shiva Pandeya,Xueqing Zhu,Kai Xu,Meixing Gui,Hongbing Wang,Ziliang Li,Qianqian Shang,Mahesh Kumar Joshi
The design of a double Z-type heterojunction represents a promising strategy in photocatalysis to achieve superior charge carrier separation, increased light-harvesting capability, and consequently, a higher photocatalytic reaction rate. Herein, a novel ternary heterojunction with direct dual Z-scheme configuration, g-C3N4/In2S3/In2O3 photocatalyst, was synthesized using a chemical bath and facile calcination process. The XRD, SEM-EDS and XPS results confirmed the successful integration of g-C3N4, In2S3, and In2O3 with preserved crystalline phases. Photocatalytic performances of the ternary composite were evaluated via two effective routes, such as methyl orange (MO) degradation and hydrogen (H2) generation, benchmarked against the pristine g-C3N4 and the g-C3N4/In2S3 and In2S3/In2O3 binary composite, revealing that the in-situ growth of In2O3 onto the g-C3N4/In2S3 composite contributed to the remarkable enhancement of their photocatalytic activity. The photodegradation process follows the pseudo-first order reaction kinetics. The ternary g-C3N4/In2O3/In2S3 heterojunction exhibited 94.8% degradation efficiency of MO with a corresponding rate of 23.4×10-3 min-1, 4.8 times higher than the pristine g-C3N4, and excellent photocatalytic activity in five consecutive cycles. Similarly, the g-C3N4/In2S3/In2O3 heterojunction exhibited a rate constant of ~2.4 mmol h−1 g−1 for H2 generation. This superior enhancement was attributed to the dual Z-scheme charge transfer mechanism. The formation of direct dual Z-scheme heterojunction improved the light harvesting, minimized electron hole recombination, and efficiently facilitated the separation of charge carriers. This work provided a mechanistic insight for engineering multifunctional heterojunction for environmental remediation and pollutant mitigation.
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BIORESOURCE TECHNOLOGY
·
2025
·
IF 9
Yu Tian,Jiangbo Li,Dengyang Wang,Shaojie Wang,Haijia Su
Biological hydrogen production via dark fermentation is a promising and sustainable approach for renewable energy generation. However, its practical application is hindered by the inefficient utilization of carbon substrates, as a large proportion is diverted into inhibitory by-products such as volatile fatty acids (VFAs). To overcome this limitation, this study developed an integrated one-pot system, combining dark fermentation with enzyme-catalyzed esterification, which converts VFAs into high-value esters while enhancing hydrogen production. Key influence factors, including lipase type, dosage, pH, and extractant type, were systematically evaluated. Under optimal conditions, cumulative hydrogen production reached 3631 ± 154 mL/L, a 58.21 % increase compared to the non-esterified group, while butyl butyrate (BB) production in extraction reached 8.66 ± 0.06 g/L. The carbon flow analysis demonstrated that approximately 7.60 % of the carbon from the consumed starch was converted into BB. Furthermore, a fed-batch fermentation resulted in simultaneous hydrogen and BB production, achieving a final BB production of 22.44 ± 0.36 g/L. Microbial community analysis indicated that the esterification strategy promoted the enrichment of Clostridium species, known for their pivotal role in hydrogen production. Under the esterification strategy, KEGG based functional genes analysis showed a notable enrichment in the relative abundances of genes associated with glycolysis and hydrogenase functions. This innovative approach not only alleviates VFAs inhibition but also provides a theoretical and technical foundation for the high-value utilization of VFAs, offering considerable potential for industrial applications.
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PLACENTA
·
2024
·
IF 3
Gaoying Wang,Ruirui Dong,Haijian Zhao,Ningzhen Ye,Jing Wang,Jing Cheng,Xinrui Shi,Liang Luo,Ting Zhang
Background Abnormal bile acid metabolism leading to changes in placental function during pregnancy. To determine whether endoplasmic reticulum protein 29 (ERp29) can mediate the pregnancy effects of cholestasis by altering the level of trophoblast cell apoptosis. Methods ERp29 in serum of 66 intrahepatic cholestasis of pregnancy (ICP) pregnant women and 74 healthy were detected by ELISA. Subcutaneous injection of ethinyl estradiol (E2) was used to induce ICP in pregnant rats. Taurocholic acid (TCA) was used to simulate the ICP environment, and TGF-β1 was added to induce the epithelial mesenchymal transformation (EMT) process. The scratch, migration, and invasion test were used to detect the EMT process. ERp29 overexpression/knockdown vector were constructed and transfected to verify the role of ERp29 in the EMT process. Downstream gene was obtained through RNA-seq. Results Compared with the healthy pregnant women, the expression levels of ERp29 in serum of ICP pregnancy women were significantly increased (P < 0.001). ERp29 in the placenta tissue of the ICP pregnant rats increased significantly, and the level of apoptosis increased. The placental tissues of the ICP had high expression of E-cadherin and low expression of N-cadherin, snail1, vimentin. After HTR-8/SVneo cells were induced by TCA, EMT was inhibited, while the ERp29 increased. Cell and animal experiments showed that, knockdown of ERp29 reduced the inhibition of EMT, the ICP progress was alleviated. Overexpression of FOS salvaged the inhibitory effects of ERp29 on cell EMT. Discussion The high level of ERp29 in placental trophoblast cells reduced FOS mRNA levels, inhibited the EMT process and aggravated the occurrence and development of ICP.
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Bioanalysis
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2016
·Vol. 8
·期 13
·
IF 2.813
Qinghua Wang,Yan Wen,Tianyi Xia,Xiaojuan Xiong,Shouhong Gao,Chunhua You,Xia Tao,Feng Zhang,Wansheng Chen
Aim: Quantification of amino acids in human plasma has become an important and essential analysis parameter in life science. In this paper, we developed a targeted UHPLC–MS/MS method for 18 amino acids in the renal transplant patients. Methods & results: Plasma in small volume (150 μl) was pretreated by a one-step protein precipitant extraction for analysis. Detection was executed by MS/MS in the MRM mode. Assays were validated according to current bioanalytical guidelines, with good linearity (R > 0.99), intraday and interday precision (CV < 11.6%, RE ≤ ± 14.8%), extraction recovery (between 77.4 and 117.6%), matrix effect (73.3–118.0%) and stability (RE≤ ±14.7%). Conclusion: The method was successfully applicable for amino acid analysis in the renal transplant patient.
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FOOD CHEMISTRY
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2023
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IF 8.8
Zhuye Shang,Qingtao Meng,Dihua Tian,Yue Wang,Zexi Zhang,Zhiqiang Zhang,Run Zhang
We report the development of a red-emitting fluorescence probe ( XDS ) for hydrogen sulfide (H 2 S) detection in biosystems, real-world food samples, and application of this probe for monitoring of H 2 S production during food spoilage. The XDS probe is developed by coupling of coumarin derivative to rhodanic-CN through a H 2 S responsive C C bond. Remarkable fluorescence quenching of XDS is observed as a result of the response to H 2 S. Semi-quantitative detection of H 2 S in three real-world water and two beer samples and monitoring of H 2 S production during food spoilage in real-time by “naked-eye” and smartphone colorimetric analysis are then achieved using XDS as the probe. Moreover, XDS is low toxicity, allowing it being used for visualizing endogenous and exogenous H 2 S in vivo in a mouse model. It is expected that the successful development of XDS could provide an effective tool for investigating the roles of H 2 S in biomedical system and for future food safety evaluation.
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JOURNAL OF HAZARDOUS MATERIALS
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2019
·
IF 7.65
Tianqi Zhao,Kun Zhang,Junwei Chen,Xiaobai Shi,Xu Li,Yanli Ma,Guizhen Fang,Shiyu Xu
To mitigate the serious ecological problems and risks to human health that are posed by heavy metals in the soil, it is important to enhance the efficiency of heavy metal extraction by washing contaminated soil using chemical methods. Secondary pollution of soil by chemical chelating agents has, however, brought a new threat to the environment. Here, we describe a biodegradable LBPAA (lignin based poly(acrylic acid)) composite that was designed as a chelating agent to wash soil contaminated with Cu 2+ , Zn 2+ , Cd 2+ and Pb 2+ ions. Extraction and ion transfer of heavy metal ions by the LBPAA composite improved the remediation rate of contaminated soil during water leaching. After washing five times, the LBPAA-assisted elution process reduced the amount of Cu 2+ , Zn 2+ , Cd 2+ and Pb 2+ ions in contaminated soil to 22.57%, 52.60%, 13.63% and 17.95%, respectively. These values are 2.39-fold, 5.04-fold, 5.04-fold and 1.31-fold, respectively, better than elution with deionized water. Additionally, LBPAA is able to sequester Cd 2+ and Pb 2+ ions from the contaminated soil and transfer them to the eluent. In summary, this work provides a safe, environmentally friendly and sustainable remediation strategy for heavy metal-contaminated soil and demonstrates a new application for lignin in the field of soil remediation.
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BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2010
·Vol. 86
·期 1
·38-42
·
IF 0.992
Yang Zhong-Zhi,Li Yong-Fang,Li Zhi-Chun,Gu Yu-Mei,Zhu Ya,Zhang Zhan-Jun
Five higher Chlorinated Benzenes (CBs), include Hexachlorobenzene (HCB), pentachlorobenzene (PeCB) and three isomers of tetrachlorobenzens, were analyzed in 20 outdoor dust samples collected from a fast developing city, Xinxiang. Only HCB was detected in all outdoor dust samples. The dominate part of the CBs residue in most samples was HCB and the mean HCB concentration (4.13 ng/g dry weight) in this study was in the range of global background soil HCB levels (ranging from 0.010 to 5.20 ng/g dry weight). The higher ratios of HCB/PeCB in the outdoor dust samples in the present study indicate that pesticide application may be an important source of HCB in China.
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MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Libin Wan,Huoliang Gao,Haidong Gao,Ge Yan,Fayun Wang,Yong Wang,Mantang Chen
A novel dummy molecularly imprinted polymer (MIP) was prepared for highly specific recognition of vardenafil. Sildenafil was used as the template, and Fe-based metal-organic gel was in situ formed and acted as structural modulator and porogenic agent. The obtained MIP was demonstrated to have stronger binding affinity and higher binding capacity towards vardenafil than non-imprinted polymer (NIP). Adsorption processes of vardenafil on MIP and NIP were fitted well by Freundlich isotherm model. Combined with LC-MS analysis, a miniaturized solid phase extraction (SPE) method using MIP as the adsorbent and using a syringe connected with a nylon membrane filter as the adsorbent container was developed. Under the optimal extraction parameters, trace vardenafil has been extracted by the developed method from the spiked water sample with satisfied recovery of 104%, and the intra- and inter-day relative standard deviations (RSDs) were below 7.3%. The linear range was 10–1000 ng mL −1 (R 2 = 0.9996), and the limit of detection (LOD, S/N = 3) and limit of quantification (LOQ, S/N = 10) were 1.8 and 6.0 μg L −1 , respectively. Finally, the proposed MIP-SPE-LC/MS method was applied to discover vardenafil from health care products (HCPs) using standard addition method. The repeatability for both HCPs was below 6.3% (RSD values). Analysis results implied that the developed MIP-SPE-LC/MS method is green, convenient and useful to monitor the illegal additives in HCPs.
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JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
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2019
·
IF 1.17
Lei Xin,Xiao-Hui Liu,Jun Yang,Hong-Yi Shen,Guang Ji,Xiu-Feng Shi,Yan Xie
The purpose of this study was to investigate the absorption properties of isorhamnetin (IS), quercetin (QU), and kaempferol (KA) in total flavones of Hippophaë rhamnoides L. (TFH) by an in situ single-pass intestinal perfusion model. The results indicated that IS, QU, and KA in TFH were absorbed site-dependently, and both enterohepatic circulation and intestinal flora could participate in their absorption processes. The absorption mechanisms of IS, QU, and KA in TFH were involved in both passive diffusion and active transport, and the mediation of efflux transporter multidrug resistance-associated proteins (MRPs) should not be neglected.
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WATER RESEARCH
·
2023
·
IF 12.8
Shiyu Cao,Guangming Zhan,Kai Wei,Biao Zhou,Hao Zhang,Tingjuan Gao,Lizhi Zhang
The mechanistic study of soil and groundwater remediation in petroleum contaminated lands significantly demands rapid qualitative and quantitative identification of petroleum substances. However, most traditional detection methods cannot provide the on-site or in-situ information of petroleum compositions and contents simultaneously even with multi-spot sampling and complex sample preparation. In this work, we developed a strategy for the on-site detection of petroleum compositions and in-situ monitoring of petroleum contents in soil and groundwater using dual-excitation Raman spectroscopy and microscopy. The detection time was 0.5 h for the Extraction-Raman spectroscopy method and one minute for the Fiber-Raman spectroscopy method. The limit of detection was 94 ppm for the soil samples and 0.46 ppm for the groundwater samples. Meanwhile, the petroleum changes at the soil-groundwater interface were successfully observed by Raman microscopy during the in-situ chemical oxidation remediation processes. The results revealed that hydrogen peroxide oxidation released petroleum from the interior to the surface of soil particles and then to groundwater during the remediation process, while persulfate oxidation only degraded petroleum on the soil surface and in groundwater. This Raman spectroscopic and microscopic method can shed light on the petroleum degradation mechanism in contaminated lands, and facilitate the selection of suitable soil and groundwater remediation plans.
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