ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2022
·Vol. 415
·期 5
·867-874
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IF 4.478
Zhang Hong,Wang Yuli,Lin Yingtong,Chu Wenjuan,Luo Zhen,Zhao Mingqin,Hu Jiandong,Miao Xiangmin,He Fan
Ochratoxin A (OTA) poses severe risks to the environment and human health, making the development of an accurate and sensitive analytical method for OTA detection essential. In this study, a catalytic hairpin assembly (CHA)–based Förster resonance energy transfer (FRET) aptasensor was developed to detect OTA using carbon quantum dots (CDs) and 6-carboxy-fluorescein (FAM) as dual signal readout. In the presence of OTA, the aptamer specifically interacted with OTA to release the helper DNA (HP), which could open the hairpin structure of FAM-labeled hairpin DNA 1 (H 1 -FAM) modified on the surface of gold nanoparticles (AuNPs). CHA between H 1 -FAM and hairpin H 2 labeled with CDs (H 2 -CDs) can release HP for the next cycle, resulting in the occurrence of FRET with CDs as the energy donor and FAM as the energy acceptor. According to the ratio of F CDs /F FAM , the proposed aptasensor showed a wide linear range from 5.0 pg/mL to 3.0 ng/mL and a low detection limit of 1.5 pg/mL for OTA detection. Moreover, satisfactory results were obtained for OTA detection in rice, suggesting the potential application of this sensor in food safety analysis.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 41
·19666-19680
·
IF 4.682
Zhang Lei,Wang Jinquan,Zhang Jin,Hou Linxi,Ye Dezhan,Jiang Xiancai
In this paper, we fabricated a tough, anti-freezing (freezing point of − 25.5 °C) and ionic conductive carboxymethyl chitosan (CMCS)-based double-network (DN) hydrogel by a free radical polymerization and Al 3+ coordination. In greater detail, the PAM/CMCS-Al 3+ hydrogel was composed of Al 3+ -coordinated CMCS network and covalently crosslinked PAM network. The hybrid DN structure of PAM/CMCS-Al 3+ hydrogel gave rise to high mechanical properties (tensile strength up to 2.15 MPa, elongation at break up to 1741%, toughness of 13.2 MJ/m 3 and elastic modulus of 1.43 MPa) and good fatigue resistance. PAM/CMCS-Al 3+ hydrogel not only exhibited the capacity to conduct electricity via free ions, but also had good strain sensitivity (GF = 2.09, 0–200% strain and fast response time of 234 ms). Based on the high strain sensitivity, PAM/CMCS-Al 3+ hydrogel could be used to assemble a flexible strain sensor with the ability to precisely detect human motions.Kindly check and confirm the edit made in the title.We agree with the edit made in the title.
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JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2022
·Vol. 31
·期 1
·287-296
·
IF 4.705
Xie Shiyu,Xu Bo,Yuan Li,Zhao Yaxin,Ma Na,Wang Yaomin,Liu Dagang,Xiang Aimin,Ouyang Yuge,Tian Huafeng
Zein as a natural protein had been widely used in various fields due to its biodegradability and biocompatibility. However, its sensitivity to humidity and poor mechanical performance limited its application in practice. In this study, zein-based composite nanofibers loaded with curcumin were prepared by electrospinning assisted with polyvinyl alcohol (PVA). The filtration efficiency of the modified nanofibers to the particles with diameters larger than 0.5 μm was all above 98%. The loaded curcumin interacted with protein molecular chains to form a network structure within tightly connected nanofibers, which exhibited excellent moisture resistance and good adhesion to cellulose paper towels used as air filter substrates. Meanwhile, its tensile strength had also been enhanced vastly to 0.72 MPa compared with the initial tensile strength of 0.21 MPa. This study provides a new electrospinning strategy for the preparation of zein-based composite nanofibers that can be widely utilized in air filtration with high moisture resistance.
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TRIBOLOGY LETTERS
·
2019
·Vol. 67
·期 1
·1-17
·
IF 2.235
Zhao Yuanliang,Qi Xiaowen,Zhang Wenli,Fan Bingli,Yang Qingxiang
The effects of copper nanoparticles (Cu) located in different regions of polytetrafluoroethylene/polyimide (PTFE/PI) blends on the morphology, thermal, mechanical, and tribological properties of PTFE composites were studied. Region one is PI phases (the corresponding composites is denoted as PPC), region two is PTFE phases and the interface between PTFE and PI (the corresponding composites is denoted as PPC-m). Results indicate that the incorporation of Cu nanoparticles into PI phases improves the dispersibility and homogeneity of PI phases. As a consequence, the crystallinity of PTFE composites is reduced, but compressive strength and modulus of PTFE composites are enhanced by 50.3% and 14.4% respectively. In addition, self-lubricity and wear resistance of PTFE composites are improved by 10.4% and 50.5%. Scanning electron microscopy (SEM) demonstrates that PI phases with Cu nanoparticles hinder the direct contact between PTFE matrix and counterparts. However, Cu nanoparticles located in PTFE matrix and the interface between PTFE and PI deteriorates the dispersibility and homogeneity of PI phases, which lead to the poor mechanical and tribological performance of PTFE composites.
研究领域:
材料科学
·
纳米技术
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JOURNAL OF NANOBIOTECHNOLOGY
·
2022
·Vol. 20
·期 1
·1-16
·
IF 9.429
Yang Guihua,Zhang Zhikun,Liu Kefeng,Ji Xingxiang,Fatehi Pedram,Chen Jiachuan
Background Bacterial infection in wounds has become a major threat to human life and health. With the growth use of synthetic antibiotics and the elevated evolution of drug resistant bacteria in human body cells requires the development of novel wound curing strategies. Herein, a novel pH-responsive hydrogel (RPC/PB) was fabricated using poly(vinyl alcohol)-borax (PB) and natural antibiotic resveratrol grafted cellulose nanofibrils (RPC) for bacterial-infected wound management.Results In this hydrogel matrix, RPC conjugate was interpenetrated in the PB network to form a semi-interpenetrating network that exhibited robust mechanical properties (fracture strength of 149.6 kPa), high self-healing efficiency (> 90%), and excellent adhesion performance (tissue shear stress of 54.2 kPa). Interestingly, the induced RPC/PB hydrogel showed pH-responsive drug release behavior, the cumulative release amount of resveratrol in pH 5.4 was 2.33 times than that of pH 7.4, which was adapted well to the acidic wound microenvironment. Additionally, this RPC/PB hydrogel exhibited excellent biocompatibility and antioxidant effect. Moreover, in vitro and in vivo results revealed that such RPC/PB hydrogel had excellent antibacterial, skin tissue regeneration and wound closure capabilities.Conclusion Therefore, the generated RPC/PB hydrogel could be an excellent wound dressing for bacteria-infected wound healing.
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Human Cell
·
2022
·Vol. 35
·期 5
·1535-1546
·
IF 4.374
Mao Dong,Zhang Xiaowei,Wang Zhaoping,Xu Guannan,Zhang Yun
Transmembrane protein 97 (TMEM97) is a conserved integral membrane protein highly expressed in various human cancers, including colorectal cancer (CRC), and it exhibits pro-tumor roles in breast cancer, gastric cancer, and glioma. However, whether TMEM97 participates in CRC progression is not fully understood. The expression of mRNA and protein was evaluated by real-time qPCR, western blotting, immunofluorescent, and immunohistochemical staining. TMEM97 functions in cell proliferation, apoptosis, migration, and invasion were assessed by CCK-8, flow cytometry, and transwell assays. The roles of TMEM97 in CRC cells in vivo was investigated using a subcutaneous xenograft model. The transcriptional regulation of TMEM97 was explored by luciferase reporter and ChIP assays. The silencing of TMEM97 inhibited migration and invasion of CRC cells in vitro and led to suppressed growth and enhanced apoptosis in CRC cells and xenografts, whereas overexpression of TMEM97 displayed opposite effects. Mechanistically, TMEM97 knockdown caused a reduction of the proliferating marker PCNA and an increase of pro-apoptotic proteins (cleaved caspase 8/3/7 and cleaved PARP) in CRC cells. TMEM97 also positively regulated the β-catenin signaling pathway in CRC cells and xenografts by modulating the phosphorylated-GSK-3β and active (non-phospho) β-catenin levels. Interestingly, YY1, a well-recognized oncogenic transcription factor, was identified to bind to the TMEM97 promoter and enhance its transcriptional activity, and silencing of TMEM97 abolished YY1-mediated pro-tumor effects on CRC cells. Our results suggest that TMEM97 is transcriptionally activated by YY1 and promotes CRC progression via the GSK-3β/β-catenin signaling pathway, providing that TMEM97 might be a novel therapeutic target for preventing CRC development.
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Microbial Cell Factories
·
2022
·Vol. 21
·期 1
·1-14
·
IF 6.352
Liu Yan,Wang Xiaoliang,Nong Sujin,Bai Zehui,Han Nanyu,Wu Qian,Huang Zunxi,Ding Junmei
Background Carbamate pesticides have been widely used in agricultural and forestry pest control. The large-scale use of carbamates has caused severe toxicity in various systems because of their toxic environmental residues. Carbaryl is a representative carbamate pesticide and hydrolase/carboxylesterase is the initial and critical enzyme for its degradation. Whole-cell biocatalysts have become a powerful tool for environmental bioremediation. Here, a whole cell biocatalyst was constructed by displaying a novel carboxylesterase/hydrolase on the surface of Escherichia coli cells for carbaryl bioremediation.Results The carCby gene, encoding a protein with carbaryl hydrolysis activity was cloned and characterized. Subsequently, CarCby was displayed on the outer membrane of E. coli BL21(DE3) cells using the N-terminus of ice nucleation protein as an anchor. The surface localization of CarCby was confirmed by SDS–PAGE and fluorescence microscopy. The optimal temperature and pH of the engineered E. coli cells were 30 °C and 7.5, respectively, using pNPC4 as a substrate. The whole cell biocatalyst exhibited better stability and maintained approximately 8-fold higher specific enzymatic activity than purified CarCby when incubated at 30 °C for 120 h. In addition, ~ 100% and 50% of the original activity was retained when incubated with the whole cell biocatalyst at 4 ℃ and 30 °C for 35 days, respectively. However, the purified CarCby lost almost 100% of its activity when incubated at 30 °C for 134 h or 37 °C for 96 h, respectively. Finally, approximately 30 mg/L of carbaryl was hydrolyzed by 200 U of the engineered E. coli cells in 12 h.Conclusion sHere, a carbaryl hydrolase-containing surface-displayed system was first constructed, and the whole cell biocatalyst displayed better stability and maintained its catalytic activity. This surface-displayed strategy provides a new solution for the cost-efficient bioremediation of carbaryl and could also have the potential to be used to treat other carbamates in environmental bioremediation.
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Science China-Chemistry
·
2026
·
IF 9.8
Li Jiaming,Dong Xue,Fan Lin,Zhang Zuwei,He Linwei,Yuan Mengjia,Shi Lingtong,Tao Ye,Lv Shasha,Li Kai,Huang Siyan,Chai Zhifang,Wang Shuao,Wang Yaxing
Proton separation is critical for a variety of industrial processes, yet achieving high selectivity for protons over metal ions remains a significant challenge. Here, we report a covalent organic framework (COF) membrane that exhibits exceptional proton-metal ion separation efficiency. The COF membrane features unique sub-nanochannels induced by interlayer shifting, which is achieved via a controlled interfacial polymerization process. These sub-nanochannels provide an optimal size for proton transport while effectively excluding larger metal ions. Additionally, the positive surface charge of the membrane induces electrostatic repulsion toward metal ions and offers abundant hydrogen donors, further enhancing proton transfer. As a result, the membrane exhibited unprecedented selectivity factors for H + /Na + , H + /Sr 2+ , H + /Mg 2+ , H + /Al 3+ , H + /La 3+ , and H + /UO
2+ 2
, with values of 127, 4273, 4738, 1.3 × 10 4 , 1.36 × 10 6 , and 6107, respectively, surpassing most existing membranes by one to three orders of magnitude. Furthermore, we demonstrate its practical application for the deacidification of highly acidic (3 M HNO 3 ) spent nuclear fuel raffinate, where, within 24 h of continuous operation, the feed acidity decreased by nearly 50%, with no detectable leakage of radioactive metal ions across 30 diverse radioactive cations.
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Communications Biology
·
2026
·
IF 5.8
Wang Yan,Zhu Yanping,Liu Ning-Hua,Zhao Fang,Cao Hai-Yan,Chen Yin,Zhang Yu-Zhong,Chen Xiu-Lan,Zhang Yu-Qiang,Gao Chao
Free trans -4-hydroxy-L-proline (T4LHyp), primarily released during the natural degradation of collagen, is absorbed and catabolised by heterotrophic bacteria, contributing to the global recycling of carbon and nitrogen. While bacterial T4LHyp catabolism is well documented, our understanding on how bacteria recognize and import T4LHyp remains limited. Here we show that HatA, the substrate binding protein (SBP) of an ATP-binding cassette transporter within the T4LHyp gene cluster of Vibrio alginolyticus 1A11092, recognizes T4LHyp during its uptake by the strain. HatA possessed significant binding affinity towards T4LHyp and L-proline and a binding signature (W 33 F 37 D 86 V 135 F 240 R 243 Y 265 N 267 Y 303 ), acting as the structural determinant for the specific ligand recognition, was defined. This signature is strictly conserved among HatA and its homologs, representing a subgroup of cluster D-II of SBPs. Genes encoding HatA-like SBPs are often located adjacent to collagenase and T4LHyp catabolic enzyme genes, and are nearly exclusively found in the genomes of Vibrio spp. in hosts and marine environments, which helps them exert pathogenicity when encountering host or environmental collagen. This study reveals a T4LHyp SBP in cosmopolitan Vibrio spp., offering better insights into the global T4LHyp catabolism and recycling driven by bacteria.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Zhao Mengfei,Zhang Jichuan,Guan Meng,Li Xuanhan,Sun Huating,Mao Kuirong,Tan Huizhu,Zhang Yijia,Meng Xandi,Cong Xiuxiu,Xin Yanbao,Wang Haorui,Yu MeiLing,Chen Si,Zhou Hongxv,Wang Xinhe,Yang Yong-Guang,Sun Tianmeng,Zhang Yuning
Immune checkpoint blockade (ICB) remains limited by the immunosuppressive tumor microenvironment (TME) and by the difficulty of coordinating therapeutic agents that act in distinct tumor compartments. In particular, checkpoint antibodies must function in the tumor stroma, whereas TME-remodeling small molecules often require intracellular access, creating an extracellular–intracellular delivery mismatch that conventional carriers rarely resolve. Here, we develop an acid-gated, esterase-amplified AND-gated dendritic nanogel (DNG) for the spatiotemporally sequential codelivery of anti-PD-1 antibody (aPD-1) and the PPARδ antagonist GSK3787. The DNG comprises a PEG corona for reversible aPD-1 adsorption and a hydrophobic dendritic core for GSK3787 encapsulation. Under the combined cues of tumor acidity and esterase activity, the nanogel undergoes progressive dissociation, triggering preferential extracellular release of aPD-1 in the tumor stroma, followed by tumor-cell internalization of the GSK3787-loaded core for intracellular drug action. This AND-gated design enables spatially resolved pharmacology rather than simple codelivery. In a melanoma model, DNG@(GSK3787 + aPD-1) markedly enhances antitumor efficacy, thereby promotes CD8⁺ T cell infiltration, and elicits systemic antitumor immune responses. Mechanistically, we identify a tumor cell-centric mode of immune remodeling and further show that the dendritic core actively governs not only small-molecule encapsulation, but also antibody retention and triggered release. Together, this work establishes a materials strategy for overcoming extracellular–intracellular delivery mismatch in combination immunotherapy. Graphical AND-gated dual-responsive nanogel enables spatiotemporally sequential extracellular delivery of aPD-1 and intracellular delivery of GSK3787, thereby overcoming the extracellular–intracellular delivery mismatch and synergistically remodeling the tumor immune microenvironment for enhanced cancer immunotherapy.
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CELLULAR AND MOLECULAR LIFE SCIENCES
·
2026
·
IF 6.5
Qu Qiao,Li Zhilong,Wang Dalu,Wu Di,Yin Hongzhuan
Colorectal cancer (CRC) remains a major contributor to cancer-related mortality worldwide. Although minichromosome maintenance protein 10 (MCM10) is known for its role in DNA replication, its involvement in CRC progression has not been fully elucidated. To identify key oncogenic drivers, RNA sequencing was performed on seven paired CRC and adjacent normal tissues, revealing significant upregulation of MCM10 (Log2FC = 2.359, P = 0.003). Public GEO datasets (GSE240623, GSE200427) further confirmed MCM10 overexpression in CRC tissues. This finding was validated in 40 paired clinical samples using qPCR and Western blotting. Prognostic relevance was assessed using hazard ratio (HR). Functional assays in vitro and in vivo were conducted to assess the effects of MCM10 on proliferation, migration, invasion, and M2 macrophage polarization. The m6A modification of MCM10 mRNA was evaluated through METTL3 and YTHDF1 interaction analysis in HCT116 cells. MCM10 was significantly upregulated in CRC tissues and associated with poor prognosis (HR = 1.54, P = 0.00077). MCM10 overexpression promoted CRC cell proliferation and invasion and was correlated with elevated M2 macrophage infiltration in tumor xenografts (6.16 ± 0.85% vs. 11.7 ± 1.13%, P < 0.05). Conditioned media from MCM10-overexpressing cells enhanced THP-1 macrophage polarization by increasing secretion of CCL2, CCL5, and IL10. Mechanistically, METTL3-mediated m6A modification stabilized MCM10 mRNA in a YTHDF1-dependent manner. MCM10 is aberrantly overexpressed in CRC and linked to worse clinical outcomes. Its oncogenic effects involve METTL3/YTHDF1-mediated mRNA stabilization and enhanced M2 macrophage polarization, suggesting a potential role in immune microenvironment modulation.
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Microsystems & Nanoengineering
·
2026
·Vol. 12
·期 1
·321
·
IF 11.1
Xue Tianhao,Lin Wanting,Zhou Jiahui,Luo Chaodan,Li Bohua,Zhang Xiaofang,Chen Ching-Jung,Liu Jen-Tsai,Zhu Guixian
Reliable electromechanical integration between disposable soft sensors and rigid hardware is crucial for wearable devices, yet physically fragile interfaces often cause severe motion artifacts and contact failures during exercise. To overcome this challenge, this study presents a mechanically robust sweat analysis platform featuring plug-and-play interconnects. Diverging from vulnerable printed inks, a scalable Flexible Printed Circuit Board (FPCB) was engineered with Electroless Nickel Immersion Gold (ENIG) terminals reinforced by a 0.1-mm Polyimide (PI) stiffener. This design ensures highly stable metal-to-metal contact under physical strain. To mitigate chloride corrosion while maintaining the high geometrical consistency of ENIG finishes, a hierarchical architecture was developed utilizing surface-modified carbon as the electrochemical transducer. The system enables accurate detection of lactate (5–80 mM, 0.04046 μA/mM), Na⁺ (12.5–200 mM, 119.59 mV/decade), and equivalent impedance-based sweat rate ( < 0.23 μL/min). Crucially, these robust interconnects facilitated 51 min of uninterrupted monitoring during vigorous aerobic and anaerobic exercises. Based on the acquired data, a proof-of-concept framework mapping sweat rate against lactate concentration was established for personalized exercise intensity zoning, offering a mass-manufacturable paradigm for precision sports physiology.
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