Nature Communications
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2024
·Vol. 15
·期 1
·1-11
·
IF 14.7
Chen Chao,Ma Yinglin,Yao Kunda,Ji Qingmin,Liu Wei
Chiral metal surfaces provide an environment for enantioselective adsorption in various processes such as asymmetric catalysis, chiral recognition, and separation. However, they often suffer from limitations such as reduced enantioselectivity caused by kink coalescence and atomic roughness. Here, we present an approach using medium-entropy ceramic (MEC), specifically (CrMoTa)Si 2 with a C40 hexagonal crystal structure, which overcomes the trade-off between thermal stability and enantioselectivity. Experimental confirmation is provided by employing quartz crystal microbalance (QCM), where the electrode is coated with MEC films using non-reactive magnetron sputtering technology. The chiral nature is verified through transmission electron microscopy and circular dichroism. Density-functional theory (DFT) calculations show that the stability of MEC films is significantly higher than that of high-index Cu surfaces. Through a combination of high-throughput DFT calculations and theoretical modeling, we demonstrate the high enantioselectivity (42% e.e.) of the chiral MEC for serine, a prototype molecule for studying enantioselective adsorption. The QCM results show that the adsorption amount of L-serine is 1.58 times higher than that of D-serine within a concentration range of 0-60 mM. These findings demonstrate the potential application of MECs in chiral recognition.
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MOLECULAR BIOLOGY REPORTS
·
2026
·Vol. 53
·期 1
·1836
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IF 3.2
Zhang Hong-dou,Wang Yun-ting,Yang Ze-yu,Wang Hui-dan,Li Xi-yue,Zhang Chun-rui,Li Cheng-zhi,Li Peng
Introduction Floralozone(FL) is a medicinal compound with potential cardiovascular protective effects. Vascular endothelial growth factor receptor 1 (VEGFR1) signaling has been implicated in cardiovascular dysfunction. This study investigated whether floralozone ameliorates heart failure (HF) through regulation of VEGFR1. Methods HF was induced in rats by intraperitoneal injection of doxorubicin (DOX) for six weeks. VEGFR1 expression was analyzed by immunofluorescence and Western blotting. Oxidative stress was determined by DHE staining of myocardial ROS and serum MDA and SOD assays. Senescence associated β-galactosidase (SA-β-gal) staining was performed to evaluate the degree of myocardial aging. HE staining was used to observe cardiac morphology. Masson, argyrophilic and orcein staining were applied to detect the degree of myocardial fibrosis, and echocardiography was conducted to assess cardiac function in each group. Results Floralozone (FL) modulated VEGFR1 expression and alleviated DOX-induced oxidative stress, cardiac aging, myocardial fibrosis, and cardiac dysfunction in HF rats. The protective effects of FL on oxidative stress, cardiac aging, myocardial fibrosis, cardiac function, and VEGFR1 expression were attenuated by pharmacological activation of VEGFR1 with placental growth factor (PLGF), supporting the involvement of VEGFR1 signaling in the cardioprotective effects of FL. Discussion These findings suggest that floralozone exerts cardioprotective effects against DOX induced heart failure, at least in part, through modulation of VEGFR1 associated signaling and oxidative stress. Floralozone modulated VEGFR1 expression and was associated with reduced oxidative stress, cardiac aging, and myocardial fibrosis, ultimately improving cardiac dysfunction. However, the precise causal relationship between VEGFR1 signaling and oxidative stress remains to be determined. These findings suggest that floralozone may have therapeutic potential for DOX induced cardiac injury through modulation of VEGFR1 associated pathological processes.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-16
·
IF 15.7
Peng Wang,Chen Shu,Ma Jingyu,Wei Wenjie,Lin Naixin,Xing Jinchao,Guo Wenjing,Li Heying,Zhang Liang,Chan Kuiming,Yen Andrew,Zhu Guangyu,Yue Jianbo
The interplay between lipid droplets (LDs) and endosomes remains unknown. Here, we screen and synthesize AP1-coumarin, an LD-specific probe, by conjugating a fluorescent dye coumarin to a triazine compound AP1. AP1-coumarin labels all stages of LDs in live cells and markedly induces the accumulation of enlarged RAB5-RAB7 double-positive intermediate endosomes. The AP1-coumarin-labeled LDs contact these intermediate endosomes, with some LDs even being engulfed in them. When LD biogenesis is inhibited, the ability of AP1-coumarin to label LDs is markedly reduced, and the accumulation of enlarged intermediate endosomes is abolished. Moreover, blocking the biogenesis of LDs decreases the number of late endosomes while increasing the number of early endosomes and inhibits the endosomal trafficking of low-density lipoprotein (LDL) and transferrin. Correspondingly, interference with RAB5 or RAB7, either through knockdown or using dominant-negative mutants, inhibits LD catabolism, whereas the expression of a RAB7 constitutively active mutant accelerates LD catabolism. Additionally, CCZ1 knockdown not only induces the accumulation of intermediate endosomes but also inhibits LD catabolism. These results collectively suggest that LDs and endosomes interact and influence each other’s functions, and endosomal trafficking participates in the catabolic process of LDs to maintain lipid homeostasis.
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Nano Research
·
2024
·
IF 9.5
Wang Peng,Wang Qianjin,Wu Dengxian,Zhang Yunyang,Kang Shixiong,Wang Xucai,Gu Jiayu,Wu Hao,Xu Zhihong,Jiang Qing
Bone tissue engineering provides a promising strategy for the treatment of bone defects. Nonetheless, the clinical utilization of biomaterial-based scaffolds is constrained by their inadequate mechanical strength and absence of osteo-inductive properties. Here, we proposed to endow nano-scaffold (NS) constructed by coaxial electrospinning technique with enhanced osteogenic bioactivities and mechanical properties by incorporating biocompatible magnetic iron oxide nanoparticles (IONPs) and icaritin (ICA). Four types of nano-scaffolds (NS, ICA@NS, NS-IONPs and ICA@NS-IONPs) were prepared. The incorporation of ICA and IONPs minimally impact their surface morphological and chemical properties. IONPs enhanced the mechanical properties of NS scaffolds, including hardness, tensile strength, and elastic modulus. In vitro assessments demonstrated that ICA@NS-IONPs exhibited enhanced osteogenic bioactivities towards mouse calvarial pre-osteoblast cell line MC3T3-E1 as evidenced by detecting the alkaline phosphatase (ALP) activity level, expressions of osteogenesis-related genes and proteins as well as mineralized nodule formation. Mechanistic investigations revealed that MEK/ERK (MAP kinase-ERK kinase (MEK)/extracellular-signal-regulated kinase (ERK)) signaling pathway could offer a plausible explanation for the osteogenic differentiation of MC3T3-E1 cells induced by ICA@NS-IONPs. Furthermore, the implantation of nano-scaffolds in rat skull defects exhibited a substantial improvement in in vivo bone regeneration. Therefore, IONPs and ICA incorporated coaxial electrospinning nano-scaffolds present a novel strategy for the optimization of scaffolds for bone tissue engineering.
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Reaction Kinetics Mechanisms and Catalysis
·
2024
·
IF 1.7
Sun Min,Chu Siyu,Lv Denghong,Li Bin,Kang Liping
In order to promote the application of carbon dots as carriers in oxygen reduction reaction, CD with uniform size distribution was prepared by microwave heating using citric acid and urea as raw materials through a bottom-up synthesis method. Due to the presence of a large number of hydroxyl groups on the surface of CD. We found that the prepared CD can be used as a reducing agent and stabilizer to directly reduce PdCl 2 to obtain Pd nanoparticles under oil bath conditions. The obtained Pd/CD system has excellent catalytic effect on the electrocatalytic oxygen reduction. The results show that the half-wave potential of Pd/CD-0.1 catalyst reaches 0.83 V, which is comparable to that of commercial Pd/C, and the corresponding limiting diffusion current density is even larger than that of commercial Pd/C catalyst. After a 5000 cycles stability test, the LSV exhibits a left shift of only 19 mV.
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JOURNAL OF POLYMER RESEARCH
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2024
·Vol. 31
·期 6
·1-14
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IF 2.6
Jiang Jiacheng,He Zhen,Yin Wang,Chen Rui,He Jing,Lang Meidong
As a typical biodegradable material, poly(lactic acid) (PLA) has great development potential in fields such as packaging, textiles and biomedical applications due to its high modulus and strength as well as excellent biocompatibility. However, the inherent brittleness and poor toughness of PLA significantly limit its widespread application. In this study, a biodegradable aliphatic–aromatic copolyester called poly(ethylene adipate- co -terephthalate) (PEAT) was synthesized using the industrially well-established direct esterification method. PEAT demonstrated excellent flexibility and extremely high fracture elongation. Its fracture elongation exceeded 900%, making it a highly ductile material with exceptional toughness. Given the complementary nature of PLA and PEAT, blending PLA with PEAT becomes a natural choice for improving the performance of PLA. The influence of the addition of PEAT on the mechanical properties, thermal performance, crystallization, phase morphology and rheological behavior of the blends was thoroughly investigated. With the addition of PEAT, the fracture mode changed from brittle fracture of the neat PLA to ductile fracture of the blends as illustrated by tensile test. The best toughening effect on PLA was achieved at a PEAT content of 30 wt%, with the elongation at break increasing from 6.7% for neat PLA to 359% for the binary blend, an improvement of 53 times. Additionally, the impact strength also increased from 4.4 kJ/m 2 to 10.5 kJ/m 2 , an increase of 138%. DSC revealed that PEAT had a certain influence on the crystallization behavior of PLA. SEM showed that the PEAT toughening mechanism was primarily attributed to a shear yielding mechanism triggered by debonding cavitation.
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JOURNAL OF MATERIALS SCIENCE
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2024
·Vol. 59
·期 44
·20575-20592
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IF 3.5
Yang Yutong,Qi Wenkang,Xie Yuanyuan,Huang Chunjie,Su Qiaoqiao,Xue Xingyong,Han Yaocong,Wei Dongping
As efficient activators of peroxymonosulfate (PMS), cobalt ions play an important role in the degradation of organic pollutants. It is imperative to synthesize PMS activators from low-cost materials with uniform structures to improve the catalytic efficiency and cycle life and reduce the cost of wastewater treatment, which has practical importance. In this work, alkali-activated slag geopolymer microsphere-supported Co catalysts (Co-GMs) were synthesized via a simple solution impregnation method and used for the degradation of Rhodamine B (RhB). The Co-GM/PMS system is highly efficient, and the degradation rate of RhB (40 mg/L) reached 99.8% within only 10 min. Moreover, Co-GM has a wide pH range from 3 to 9 and good anti-interference performance for other ions, as well as low Co 2+ leaching (0.809 mg/L) and favorable reusability. Reactive oxygen species (ROS) were explored via radical quenching experiments and electron spin-resonance (ESR) spectroscopy, which revealed determined that the major ROS involved in the degradation of RhB are 1 O 2 and SO 4 ·− . The synergistic effect of free and nonfree radicals plays an important role during degradation. Moreover, four probable degradation pathways were proposed via liquid‒phase mass spectrometry (LC‒MS). The results revealed that the removal rate of total organic carbon (TOC) reached 74.2% within 60 min. This research innovatively proposed the application of geopolymer composite catalysts in advanced oxidation and developed an ultralow-cost PMS activator for highly efficient degradation of RhB, which represents a new choice for wastewater treatment. Graphical abstract
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Biomass Conversion and Biorefinery
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2024
·
IF 3.5
Ijaz Hira,Sun Shangde
The objective of this work was to utilize tiger nut oil as a new raw material to produce advanced structured lipids that are beneficial for human health. Behenic acid was also used as another raw material to make novel structured lipids. Enzyme screening on the reaction was scrutinized. The effect of different conditions (time, temperature, substrate ratio, and enzyme load) on the yield of structured lipids was evaluated and optimized by response surface methodology. Authentication of the response surface model was confirmed by the excellent acquiescence between the experimental and the predicted values of structured lipids yield. Furthermore, the kinetic model was applied based on the Ping-Pong Bi Bi method. Results showed that the novel structured lipids were successfully prepared using tiger nut oil as raw material, and Lipozyme RMIM as a green cleaner catalyst showed good performance for this reaction. The maximum yield of the product (78.8%) was obtained at a temperature of 85℃; time, 1.25 h; substrate ratio, 1:6 (w/w); and enzyme load, 12.5%. In addition, the reaction’s calculated activation energy ( Ea ) was estimated to be 17.302 kJ/mol. This study also summarized that response surface methodology helps to optimize the reaction conditions. The optimized production process offers a scalable method for creating structured lipids with specific health benefits, potentially benefitting the food, and nutraceutical industries by providing new chances for making health-promoting products. Graphical Abstract
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Journal of Inorganic and Organometallic Polymers and Materials
·
2025
·
IF 4.9
Bao Xuemei,Yang Yongcong,Zhao Tianjing,Liu Song,Zhou Wenyao,Liu Qianqian,Yu Fengjiao,Yang Yang
A modified polysulfone (PSF) ultrafiltration (UF) membrane is designed by grafting phytic acid/silver ion (PA-Ag I ) complex aggregates on the membrane surface for achieving improved separation and anti-biofouling performance. We demonstrate that PA molecules directly grafted on the PSF membrane surface through hydrogen bonding offer a suitable coordinating ability to immobilize Ag ions to form hydrophilic aggregates. One-cycle sequential assembly reinforced by a high concentration of PA is confirmed optimal for generating a compact grafting layer with better water affinity and properly narrowing the pore aperture. This modification results in a reasonable permeation flux (313.3 L m –2 h –1 bar –1 ), a high BSA rejection (93.2%) and a good antifouling property. Moreover, the PA-Ag I /PSF membrane displays excellent antibacterial and anti-adhesion properties against E. coli and B. subtilis , attributed to the gradual release of Ag ions from the moderately stable PA-Ag I complexes in a favorable aqueous environment. The PA-Ag I /PSF membrane keeps over 83% of water flux after incubation of bacteria culture for 48 h, presenting outperforming anti-biofouling durability. The low dosage of Ag ions from the PA-Ag I /PSF membrane has merits of low consumption, optimization of release profile, and alleviated environmental concerns.
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AQUACULTURE INTERNATIONAL
·
2024
·
IF 2.2
Cui Jun,Zeng Dan,Guan Peipei,Jiang Ning
Exposure to pathogens induces hyperactivate inflammatory immune responses, oxidative stress and even death in fish. Therefore, suppressing excessive inflammatory responses is a means of treating fish diseases. Scutellariae Radix (SR), an herbal medicine, has anti-inflammatory and antioxidant properties. However, the active ingredients of SR that produce a marked effect and their mechanisms are poorly understood. In this study, 32 active ingredients of SR and their 124 target genes associated with anti-inflammatory effect were screened. These target genes in zebrafish were mainly enriched in biological processes associated with response to stress and stimulus. The KEGG enrichment analysis and pathway–pathway network analysis showed that the MAPK signalling pathway and nine other pathways were the key pathways associated with anti-inflammatory effect. A protein–protein interactions (PPI) network of target genes was constructed, and AKT2 and nine other genes were identified as the hub genes by cytoHubba. Combining the above results, we constructed an active ingredient–hub protein–key pathway network, showing that wogonin-AKT2 was the key regulated module. Molecular docking and SPR-based assays were used to investigate the possible interaction of wogonin with the AKT2 protein. The infected zebrafish and grass carp treated with wogonin displayed a higher survival rate and anti-inflammatory and antioxidant effects than the infected fish without wogonin treatment. Dietary wogonin also enhanced grass carp anti-inflammatory and antioxidant effects. These findings further reveal the molecular biological mechanism by which wogonin from SR exerts anti-inflammatory effects and provide a theoretical basis for the prevention of fish diseases.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-11
·
IF 14.7
Zheng Yue,Wang Huan,Liu Yan,Liu Peiyu,Zhu Baoli,Zheng Yanning,Li Jinhua,Chistoserdova Ludmila,Ren Zhiyong Jason,Zhao Feng
The chemical transformations of methane (CH 4 ) and carbon dioxide (CO 2 ) greenhouse gases typically have high energy barriers. Here we present an approach of strategic coupling of CH 4 oxidation and CO 2 reduction in a switched microbial process governed by redox cycling of iron minerals under temperate conditions. The presence of iron minerals leads to an obvious enhancement of carbon fixation, with the minerals acting as the electron acceptor for CH 4 oxidation and the electron donor for CO 2 reduction, facilitated by changes in the mineral structure. The electron flow between the two functionally active microbial consortia is tracked through electrochemistry, and the energy metabolism in these consortia is predicted at the genetic level. This study offers a promising strategy for the removal of CH 4 and CO 2 in the natural environment and proposes an engineering technique for the utilization of major greenhouse gases.
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JOURNAL OF FLUORESCENCE
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2024
·
IF 2.6
Chen Xian,Zhang Menghan,Zheng Lang,Deng Xiaoqin,Chen Qiang,Han Wendi,Huang Zhengjun,Weng Shaohuang
The establishment of a convenient and efficient testing method is crucial and needed for the monitoring of diosmin. In this study, nitrogen doped carbon dots (N-CDs) with the particle size distribution of 2.5–5.7 nm and the average diameter of 4.1 nm were successfully synthesized using a simple strategy. N-CDs exhibited excellent and stable fluorescence performance with the quantum yield of 22.33%. Correspondingly, a fluorescence analytical method was developed for diosmin determination using N-CDs as probe. UV-vis absorbance spectroscopy and the evaluation of internal filtering parameters verified that the mechanism causing the quenching of N-CDs was an internal filtering effect (IFE). The concentration of diosmin can be directly evaluated based on the quenched fluorescence intensities. After optimizing experimental conditions, it was found that the fluorescence quenching efficiency ((F 0 -F)/F 0 ) of N-CDs exhibited a good linear relationship with the concentration of diosmin (C Diosmin ) in the range of 3.0–50 µg/mL. The limit of detection (LOD) was 0.86 µg/mL based on 3σ/slope ( n = 13). This method was successfully applied to accurately determine the content of diosmin in diosmin tablets and human plasma samples with good reproducibility. It stands out for its simplicity, speed, and acceptable determination performance.
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