MICROCHIMICA ACTA
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2020
·Vol. 187
·期 10
·1-9
·
IF 6.232
Pu Chenlu,Zhao Hongli,Gu Qinying,Zheng Yu,Lan Minbo
A selectively modified porous metal/carbon nanocomposite was fabricated to enhance the enrichment of low-abundance phosphopeptides from biological samples. The carbon matrix derived from the metal-organic framework provides a suitable pore size to allow the diffusion of peptides, while the deliberately modified metal nanoparticles within the pores enhance their interaction with the phosphopeptides. This nanocomposite shows extremely high enrichment selectivity for phosphopeptides in the MALDI-TOF MS detection, even when the molar ratio of α-casein digests versus bovine serum albumin digests was up to about 1:20,000. By combining such nanocomposite with nano-LC-MS/MS, 4556 unique phosphopeptides were identified with high selectivity (95.2%) from HeLa cell extracts. Furthermore, phosphopeptides from prostate tissue digests were also determined. A total of 277 and 1242 phosphopeptides were identified from normal and tumor tissues of a patient with prostate cancer, respectively. This indicates that phosphorylation and prostate cancer can be related to each other.
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JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 55
·期 35
·16625-16640
·
IF 3.553
Zhang Zewu,Zhou Jiamin,Wei Hanlin,Dai Yifan,Li Shijia,Shi Haojun,Xu Gang
Abstract Efficient design and preparation of cost-effective and binder-free bifunctional electrocatalysts to accelerate the hydrogen and oxygen evolution reaction (HER and OER) are critical for overall water splitting. In this work, 3D hierarchical NiFe-LDH/FeCoS 2 /CFC electrodes were synthesized for the first time. The carboxyl groups derived from acid treating promote the homogeneously coated FeCoS 2 on CFC, giving rise to the strongly coupled FeCoS 2 /CFC hybrid. The NiFe-LDH exhibited the vertical growth feature on the FeCoS 2 /CFC composite, which can efficiently expose the active edges sites. Due to unique structure and synergistic effect between the components, the NiFe-LDH/FeCoS 2 /CFC exhibits significant electrocatalytic activity and stability under alkaline environments, with overpotentials of 190 and 308 mV to achieve 10 mA cm −2 for OER and HER, respectively, providing it as a promising electrocatalyst for water splitting reaction. In addition, we deeply studied the synergistic catalytic mechanism of NiFe-LDH/FeCoS 2 /CFC, explaining the reasons leading to the improved catalytic performance of HER and OER. Graphic abstract
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CHEMICAL PAPERS
·
2020
·Vol. 75
·期 3
·873-881
·
IF 1.68
Yu Li,Gao Jinwei,Zeng Zhi,Zheng Yuhui
The precise control of phosphors with well-defined features remains a challenging issue since its property has been closely related to the shape, surface modification and particle size. The studies to develop microstructure under mild conditions will be of general interests. Herein, 2-thenoyltrifluoroacetone (TTA) has been incorporated as a viable technique to bind with surface of BaMgF 4 :Eu 3+ phosphor and such process has been proved to be an effective strategy in preparing inorganic materials. The well acceptable advantages of this way include facile synthesis steps, broaden excitation range and reduced particle size. Although lanthanide fluorides have been reported so far, there are limited studies concerning organic framework appended red phosphors. In this way, the exploitation of a simple method to assemble hybrid phosphors will be very valuable in opto-electronic industries.
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Biotechnology for Biofuels
·
2020
·Vol. 13
·期 1
·1-14
·
IF 4.815
Wang Xiang,Liu Si-Fen,Li Ruo-Yu,Yang Wei-Dong,Liu Jie-Sheng,Lin Carol Sze Ki,Balamurugan Srinivasan,Li Hong-Ye
Background Despite the great potential of marine diatoms in biofuel sector, commercially viable biofuel production from native diatom strain is impractical. Targeted engineering of TAG pathway represents a promising approach; however, recruitment of potential candidate has been regarded as critical. Here, we identified a glycerol-3-phosphate acyltransferase 2 (GPAT2) isoform and overexpressed in Phaeodactylum tricornutum.Results GPAT2 overexpression did not impair growth and photosynthesis. GPAT2 overexpression reduced carbohydrates and protein content, however, lipid content were significantly increased. Specifically, TAG content was notably increased by 2.9-fold than phospho- and glyco-lipids. GPAT2 overexpression elicited the push-and-pull strategy by increasing the abundance of substrates for the subsequent metabolic enzymes, thereby increased the expression of LPAAT and DGAT. Besides, GPAT2-mediated lipid overproduction coordinated the expression of NADPH biosynthetic genes. GPAT2 altered the fatty acid profile in TAGs with C16:0 as the predominant fatty acid moieties. We further investigated the impact of GPAT2 on conferring abiotic stress, which exhibited enhanced tolerance to hyposaline (70%) and chilling (10 ºC) conditions via altered fatty acid saturation level.Conclusion sCollectively, our results exemplified the critical role of GPAT2 in hyperaccumulating TAGs with altered fatty acid profile, which in turn uphold resistance to abiotic stress conditions.
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MOLECULAR MEDICINE
·
2020
·Vol. 26
·期 1
·1-14
·
IF 4.096
Sun Xiaowei,Liu Hao,Sun Zhongren,Zhang Beng,Wang Xinyu,Liu Tingting,Pan Tingting,Gao Ying,Jiang Xicheng,Li Hongtao
Background Acupuncture treatment possesses the neuroprotection potential to attenuate cerebral ischemia–reperfusion (I/R) injury. Endoplasmic reticulum (ER) stress has been suggested to be involved in the pathogenic mechanism of cerebral I/R injury. Whether acupuncture protects against cerebral I/R injury via regulating ER stress remains unclear. This study aimed to evaluate the role of ER stress in the neuroprotection of acupuncture against cerebral I/R injury and its underlying mechanisms.Methods Cerebral I/R injury was induced by middle cerebral artery occlusion (MCAO) in rats. Acupuncture was carried out at Baihui (GV 20), and Qubin (GB7) acupoints in rats immediately after reperfusion. The infarct volumes, neurological score, ER stress, autophagy and apoptosis were determined.Results Acupuncture treatment decreased infarct volume, neurological score and suppressed ER stress via inactivation of ATF-6, PERK, and IRE1 pathways in MCAO rats. Attributing to ER stress suppression, 4-PBA (ER stress inhibitor) promoted the beneficial effect of acupuncture against cerebral I/R injury. Whereas, ER stress activator tunicamycin significantly counteracted the neuroprotective effects of acupuncture. In addition, acupuncture restrained autophagy via regulating ER stress in MCAO rats. Finally, ER stress took part in the neuroprotective effect of acupuncture against apoptosis in cerebral I/R injury.Conclusion sOur findings suggest that acupuncture offers neuroprotection against cerebral I/R injury, which is attributed to repressing ER stress-mediated autophagy and apoptosis.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 413
·期 2
·469-478
·
IF 3.637
Yu Xiao,Yu Wanxiang,Han Xiufen,Chen Zuanguang,Wang Shumei,Zhai Haiyun
Doxorubicin has been extensively used to treat cancers, and there are recent findings that the anticancer activities can be enhanced by curcumin. Although the two compounds have native fluorescence, they can hardly be quantified directly simultaneously using the laser-induced fluorescence (LIF) detection method. To avoid complex fluorescence derivatization and introduction of interfering components, a highly sensitive double wavelength excitation source LIF (D-W-Ex-LIF) detector composed of a 445-nm and 488-nm commercial laser diode was constructed to detect them simultaneously. Rhodamine 6G was selected as an internal standard, because its fluorescence can be excited at 445 nm and 488 nm. The native fluorescence of doxorubicin and curcumin and their resolution were enhanced by introducing mixed micelles. The optimal electrophoretic separation buffer was 10 mM borate buffer containing 20 mM Triton X-100, 5 mM sodium dodecyl sulfate, and 30% (v/v) methanol at pH 9.00. Therefore, the developed method was specific, accurate, and easily operable. Its limits of detection for doxorubicin and curcumin in human urine samples were 4.00 × 10 −3 and 1.00 × 10 −2 μg/mL, respectively, and the limits of quantification were 1.00 × 10 −2 and 3.00 × 10 −2 μg/mL, respectively. The recoveries were 94.9–109.1%.
Graphical abstract
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SN Applied Sciences
·
2020
·Vol. 2
·期 11
·1-14
·
IF 0
Fan Zhinan,Zhang Chi,Ma Rongliang,Fan Li-Juan
Abstract Visualization of latent blood fingerprints (LBFPs) at violent crime scenes is very important for identifying criminals, while current reagents/methods for developing LBFPs still have more or less limitations. Here, we advance a new strategy for LBFP development based on the dye-soaked cotton pads as “ensemble” materials. The cotton pad was first soaked in the dye solution and then placed on the substrate with deposited blood fingerprint for development. After peeling off the pad, uniform and legible fingerprint patterns were obtained in all cases where different dyes and substrates were employed. The dye-soaked pads displayed superior developing effect compared with those using conventional methods (immersing, smearing and spraying). The pad-based method exhibited many other merits, such as little damage to the fingerprint patterns and less dye stains left on fingerprint and substrate, as well as the reusability. This pad-based method was also applicable for developing sebaceous latent fingerprints by replacing the small molecular dyes with fluorescent conjugated polymer nanoparticles. In all, the dye-soaked cotton pad developing protocol is feasible, cost-effective and universally applicable for developing different types of latent fingerprints on various substrates. Graphic abstract
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JOURNAL OF APPLIED ELECTROCHEMISTRY
·
2020
·Vol. 51
·期 2
·295-306
·
IF 2.384
Liu Qian,Zhang Binyan,Du Shilong,Li Lin,Liu Fuxin,Fan Weizhou,Liu Xiuhui
Abstract Excessive uptake of nitrite will damage the human health. Hence, there is a rising requirement to construction a highly sensitive electrochemical sensor for detecting the level of nitrite in food precisely. In this work, an electrochemical nitrite sensor was successfully constructed with the porous hollow carbon nanoparticles as the electrode material. The morphology and structure of the synthesized material were characterized by scanning electron microscopy, transmission electron microscope, Brunauer–Emmett–Teller, and Fourier transform infrared spectroscopy. Meanwhile, the electrochemical behavior of nitrite was studied by cyclic voltammetry and amperometry ( i − t ). As a result, the as-constructed sensor exhibited high electrocatalytic activity, high sensitivity (275.7 µA mM −1 cm −2 ), wider linear range (0.037–6950 μM), and lower limit of detection (10.41 nM) toward nitrite oxidation. Finally, the sensor was successfully applied to the detection of nitrite in pickle directly, indicating its feasibility for practical applications. The results showed that the porous hollow carbon nanospheres hold great prospect in sensitive detection of nitrite. Graphic abstract
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IONICS
·
2020
·Vol. 26
·期 12
·6371-6378
·
IF 2.394
Xu Haoyan,Han Chuanlong,Liu Sicheng,Hao Xin,Rao Yu,Gong Zexin,Sun Zhuangzhi
Due to the efficiency of ion movement is greatly affected by moisture environment, this article explores the effects of the changing the moisture content of the self-degraded ionic hydrogel-based electrolyte layer made of sodium alginate and chitosan on the improvement of the electromechanical properties of the soft ionic actuator. Specifically, sodium alginate-chitosan ionic hydrogel-based soft ionic actuator is fabricated and analyzed by voltammetry analysis, AC impedance, charge and discharge, electrical conductivity, displacement, and output force. Furthermore, the further application of this soft ionic actuator on bionic flytrap is developed and discussed. The results show that soft ionic actuator with the moisture content of 78.64% has the best electromechanical properties. Furthermore, soft ionic actuators with different moisture contents show different degrees of deflection due to the shift of the anions to the cathode under charged conditions. Therefore, it can be found that moisture content is beneficial to improve the electromechanical properties of ionic hydrogel-based soft artificial muscle.
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 11
·1-9
·
IF 6.232
Wang Yue,Fang Xi,Yin Huanshun,Zhou Yunlei,Yang Yue,Ai Shiyun
A novel photoelectrochemical immunosensor has been constructed for the determination of methylated RNA. MoS 2 nanosheets with large specific area were employed as photoactive material, gold nanoparticles were used as signal amplification unit and immobilization matrix of 4-mercaptophenylboronic acid, anti-m 6 A antibody was adopted as methylated RNA recognition reagent, and poly(U) polymerase–mediated RNA chain extension and Ru(NH 3 ) 6 3+ were used as assisted signal amplification unit. With the sensitization effect of Ru(NH 3 ) 6 3+ , the photoactivity of WS 2 nanosheets was improved greatly, which also improved the sensitivity. Using visible-light excitation and ascorbic acid as electron donor, the sensitive determination of methylated RNA was achieved by monitoring the photocurrent change with different concentrations of methylated RNA. This photoelectrochemical immunosensor has a wide linear relationship with methylated RNA concentration from 0.05 to 35 nM under optimal experimental conditions. The low detection limit of 14.5 pM was realized based on 3σ criterion. In addition to the good selectivity, this sensor also presents high reproducibility with a relative standard deviation of 1.4% for the photocurrent of seven electrodes. The applicability of the developed method was also investigated by detecting the level of methylated RNA in corn seedling leaves with and without sulfadiazine treatment.
Graphical abstract A novel photoelectrochemical immunosensor was developed for methylated RNA detection using the photoactive material of MoS2 and poly(U) polymerase–mediated RNA chain extension.
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International Journal of Minerals Metallurgy and Materials
·
2020
·Vol. 27
·期 9
·1216-1225
·
IF 1.713
Wei Wang,Yue Hong-rui,Xue Xiang-xin
This study investigated the interdiffusion of calcium ferrite/calcium titanate system in the time range of 0–120 min by the diffusion couple method in a CO/N 2 reducing atmosphere at 700°C. The results show that after the diffusion reaction occurred, no longitudinal agglomerations were present on the substrate surface on the calcium titanate side. When the diffusion time was increased to 105 min, a net vacancy flow from calcium titanate to calcium ferrite might have occurred, causing the surface of the calcium ferrite substrate to collapse. The thickness of the diffusion layer of the calcium ferrite/calcium titanate system was about 17–48 µm, which conforms to the parabolic law of diffusion. The diffusion coefficient and the Ti 4+ concentration in the calcium ferrite/calcium titanate system are related. This shows an increase in the diffusion coefficient with the increase of Ti 4+ concentration, and the diffusion coefficient value was in the range of 10 −12 –10 −11 cm 2 ·s −1 .
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Nature Communications
·
2021
·Vol. 12
·期 1
·1-8
·
IF 14.919
Huang Chaofeng,Wen Yaping,Ma Jin,Dong Dandan,Shen Yanfei,Liu Songqin,Ma Haibo,Zhang Yuanjian
Covalently bonded carbon nitride (CN) has stimulated extensive attention as a metal-free semiconductor. However, because of the complexity of polymeric structures, the acquisition of critical roles of each molecular constituent in CN for photocatalysis remains elusive. Herein, we clarify the fundamental active units of CN in photocatalysis by synthesizing CN with more detailed molecular structures. Enabled by microwave synthesis, the as-prepared CN consists of distinguishable melem (M1) and its incomplete condensed form (M2). We disclose rather than the traditional opinion of being involved in the whole photocatalytic processes, M1 and M2 make primary contributions in light absorption and charge separation, respectively. Meanwhile, oxygen molecules are unusually observed to be activated by participating in the photoexcited processes via electronic coupling mainly to M2. As a result, such CN has a higher activity, which was up to 8 times that of traditional bulk CN for photocatalytic oxidation of tetracycline in water.
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