Nature Communications
·
2024
·Vol. 15
·期 1
·1-14
·
IF 14.7
Su Xiaoxuan,Huang Xinrong,Zhang Yiyue,Yang Leyang,Wen Teng,Yang Xiaoru,Zhu Guibing,Zhang Jinbo,Tang Yijia,Li Zhaolei,Ding Jing,Li Ruilong,Pan Junliang,Chen Xinping,Huang Fuyi,Rillig Matthias C.,Zhu Yong-guan
The estuarine plastisphere, a novel ecological habitat in the Anthropocene, has garnered global concerns. Recent geochemical evidence has pointed out its potential role in influencing nitrogen biogeochemistry. However, the biogeochemical significance of the plastisphere and its mechanisms regulating nitrogen cycling remain elusive. Using 15 N- and 13 C-labelling coupled with metagenomics and metatranscriptomics, here we unveil that the plastisphere likely acts as an underappreciated nitrifying niche in estuarine ecosystems, exhibiting a 0.9 ~ 12-fold higher activity of bacteria-mediated nitrification compared to surrounding seawater and other biofilms (stone, wood and glass biofilms). The shift of active nitrifiers from O 2 -sensitive nitrifiers in the seawater to nitrifiers with versatile metabolisms in the plastisphere, combined with the potential interspecific cooperation of nitrifying substrate exchange observed among the plastisphere nitrifiers, collectively results in the unique nitrifying niche. Our findings highlight the plastisphere as an emerging nitrifying niche in estuarine environment, and deepen the mechanistic understanding of its contribution to marine biogeochemistry.
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Applied Biological Chemistry
·
2024
·Vol. 67
·期 1
·1-17
·
IF 2.3
Xue Jun,Gao Yu Ping
Acute myocardial infarction (AMI) has been treated via injectable hydrogels and biomaterial patches invented using tissue engineering advancements over the past decade. Yet the curative potential of injectable hydrogels and stem cells is limited. Here, we propose the development of an injectable and conductive hydrogel composed of oxidised macromolecular hyaluronic acid and chitosan-grafted aniline tetramer polymeric components. In an attempt to enhance the therapeutic potential of AMI therapy, mesenchymal stem cells derived from human umbilical cord blood (HUCB-MSC) have been integrated into the formulation of a conductive hydrogel. For reliable connection to the beating hearts, the hydrogel exhibited suitable adhesive properties. Hydrogel’s potent biocompatibility was determined by in vitro investigations of cell viability and proliferation of NRCMs and H9C2 cardiomyocytes. After myocardial injection, longer HUCB-MSCs survival length, cardiac functioning, and histology in SD rat myocardium were demonstrated, greatly associated by up-regulation and downregulation of cardiac-related relative gene expressions of angiogenic factors and inflammatory factors, respectively. The injectable hydrogel that contained HUCB-MSCs substantially enhanced the therapeutic benefits, indicating a potentially beneficial therapeutic approach to AMI therapy.
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MICROCHIMICA ACTA
·
2024
·Vol. 192
·期 1
·1-11
·
IF 5.3
Tian Siyu,Wang Jiahui,Jie Yu,Ding Zhu,Wang Xiao,Wang Jijiang,Hou Xiangyang
A novel nanobiosensor was constructed by in situ locating nanometer MnO 2 particles with controllable size and morphology in a Zr-MOF substrate to serve as an electrochemical probe. The synergistic effect of the two components, Zr-MOFs with high specific surface area and compatibility as a carrier for MnO 2 , resulted in improved electrochemical activity and excellent electrochemical identification performance for the MnO 2 @Zr-MOF/GCE biosensor. Under optimized experimental conditions and using CV and DPV technology, the biosensor showed a wide linear detection range (2–200 μM), a low detection limit (2.577 × 10 −8 M), a recovery range (106.26–115.01%), and maximum relative standard deviation (5.155) for tetracycline (TC) identification. The recognition mechanism of the sensor was investigated adopting Laviron adsorption theory. The applicability of the sensor was verified through practical measurements. Overall, the MnO 2 @Zr-MOF/GCE sensor possesses the advantages of fast analysis speed, high sensitivity, high selectivity, and simple operation, making it suitable for detecting trace amounts of TC in food. Graphical abstract
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Drug Delivery and Translational Research
·
2024
·
IF 5.7
Zhang Wei,Zhang Qian,Yang Yuhan,Chen Yangyi,Wei Jinbin,Lu Fenglai,Li Dianpeng
A novel multi-functional micelle delivery system was developed for enhancing the oral absorption of paclitaxel (PTX). The delivery carriers were constructed by modifying chitosan-stearic acid (CS-SA) micelles with L-carnitine (LC) and co-encapsulating quercetin (Que), and the PTX-loaded micelles were prepared by film-sonication dispersing technique. The as-prepared micelles showed homogeneous spherical shapes with a small particle size of 148.3 ± 1.7 nm, high drug loading of 7.05% and low critical micelle concentration (CMC) of 16.89 µg/ml. Compared to the in-house PTX formulation similar to the commercial injection Taxol™, the target PTX-loaded micelles had obvious sustained-release effects and exhibited an oral relative bioavailability of 168.08%. The cellular uptake studies of Caco-2 cells confirmed the micellar modification of LC and the co-loading of Que played important roles in promoting the absorption of drug loaded in micelles. The CYP3A4 enzyme test demonstrated the micelles had an inhibitory effect on the metabolic enzyme due to the presence of Que. These findings confirmed the potential of the multi-functional chitosan polymeric micelles based on synergistic effect as an effective oral delivery system. Graphical abstract
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-13
·
IF 15.7
Peng Qiannan,Zhao Cheng,Wang Xiaopeng,Cheng Kelin,Wang Congcong,Xu Xihui,Lin Lu
Lignin, as the abundant carbon polymer, is essential for carbon cycle and biorefinery. Microorganisms interact to form communities for lignin biodegradation, yet it is a challenge to understand such complex interactions. Here, we develop a coastal lignin-degrading bacterial consortium (LD), through “top-down” enrichment. Sequencing and physiological analyses reveal that LD is dominated by the lignin degrader Pluralibacter gergoviae (>98%), with additional rare non-degraders. Interestingly, LD, cultured in lignin-MB medium, significantly enhances cell growth and lignin degradation as compared to P. gergoviae alone, implying a role of additional outliers. Using genome-scale metabolic models, metabolic profiling and culture experiments, modeling of inter-species interactions between P. gergoviae , Vibrio alginolyticus , Aeromonas hydrophila and Shewanella putrefaciens , unravels cross-feeding of amino acids, organic acids and alcohols between the degrader and non-degraders. Furthermore, the sub-population ratio is essential to enforce the synergy. Our study highlights the unrecognized role of outliers in lignin degradation.
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RESEARCH ON CHEMICAL INTERMEDIATES
·
2024
·Vol. 50
·期 6
·2743-2761
·
IF 2.8
Lin Yingzi,Chen Jing,Chen Junjie,Chen Lei,Yang Hong,Sun ShengBo,Sun Ningning,Weng Qixuan,Tian Shiqi
To address the disadvantage of poor degradation effect of Fe 2+ /peroxydisulfate (PDS) system, a co-catalyst was introduced in this study to construct a molybdenum (Mo)/Fe 2+ /PDS system for the degradation of Acid Orange 7 (AO7), and the degradation rate could reach 95.67%. The effects of different parameters (initial pH, Fe 2+ concentration, etc.) on the degradation effect of AO7 in this system were investigated, and the optimal degradation conditions were obtained by combining cost analysis. Cycling experiments showed that Mo had good stability. The co-catalytic mechanism of Mo powder was determined using X-Ray Diffraction, Scanning Electron Microscope, and X-ray Photoelectron Spectroscopy. The effects of different inorganic anions on AO7 were discussed: Cl − and NO 3 − had little effect on degradation, while SO 4 2− and HCO 3 − both inhibited the removal of AO7 from the system to different degrees, with HCO 3 − showing a greater inhibition than SO 4 2− . The main active radicals were identified as sulfate radical (SO 4 ·− ) and hydroxyl radical (·OH). In addition, intermediates were identified, and the attack sites were determined by combining the highest occupied molecular orbital/lowest unoccupied molecular orbital energy levels and the Fukui index to hypothesize the degradation pathways. Toxicity indicators such as the mutagenicity of AO7 and intermediates were evaluated, and the overall trend of toxicity was decreasing. This study is expected to provide an efficient treatment process for dye wastewater.
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Journal of Food Measurement and Characterization
·
2024
·
IF 2.9
Li Shen,Chen Xiao,Zhou Yongsheng,Pan Mingxuan,Zhou Xinghu,Hu Yadong,Zhao Liyan
Heat treatment is widely applied in food processing, but its significant effects on flavor are often overlooked. This study explored how heat treatment impacts the flavor compounds of Antarctic krill. The volatile substances and flavor profiles of Antarctic krill post steaming or hot air drying were analyzed using headspace solid-phase microextraction, gas chromatography-mass spectrometry (HS-SPME-GC-MS), gas chromatography-ion mobility spectrometry (GC-IMS), and electronic tongue detection techniques. Free amino acids (FAAs) content, the main flavor precursors, were quantified and compared. The results showed that the FAAs content of SK was the lowest at 48.44 ± 2.10 mg/g db, while the FAAs content of HD_105 was the highest at 67.42 ± 1.77 mg/g db. Drying amplified the flavor intensity of umami and bitter amino acids, while steaming significantly increased the content of the sweet amino acid proline ( p < 0.05). The electronic tongue discerned that drying had more pronounced effects than steaming on saltiness, umami, bitterness, and astringency. By combining the volatile organic compounds (VOCs) fingerprints with the results of the GC-MS analysis of five samples, it was observed that the richness in fat and aromatic taste of shrimp powder after hot air drying at 60℃ (HD_60) and 80℃ (HD_80) was enhanced. Krill dried at 105℃ (HD_105) predominantly exhibited a roasted flavor profile, while steamed krill (SK) displayed minimal alterations in volatile flavors. In summary, steaming increased the sweet amino acid content in krill, while drying enhanced krill’s aroma characteristics. This study furnishes valuable insights for the subsequent processing and utilization of Antarctic krill to augment its flavor quality.
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RARE METALS
·
2024
·
IF 9.6
Tu Zhuo,Wan Yong,Ge Juan,Li Chen-Chen,Liang Tao,Li Zhen
Abstract Photodynamic therapy (PDT), with high spatiotemporal selectivity, minimal side effects and less drug resistance, has emerged as a promising approach for the treatment of cancer. However, inadequate penetration depth and poor efficacy seriously restrict its practical application. To address these challenges, we developed a combined therapy platform (RENP@C/D-FA) based on rare-earth-doped nanoparticles (RENPs) with a structure of NaErF 4 :Tm(0.5%)@NaYF 4 , on which photosensitizer Ce6 (chlorin e6) and chemo-therapeutic drug (DOX) (doxorubicin) were loaded by encapsulating with an amphiphilic polymer distearoyl phosphatidylethanolamine-polyethylene glycol-folic acid (DSPE-PEG-FA). RENPs with NaErF 4 as the matrix possess multiple excitation/emission peaks, enabling activation of PDT under near-infrared-IIb (NIR-IIb, 1500–1700 nm) excitation and luminescence imaging in NIR-IIb region by absorbing 808 nm near-infrared photons. Furthermore, DOX can be selectively released in the acidic microenvironment of tumors. Benefiting from the tumor targeting capability of folic acid, this platform can successfully ablate tumor cells and inhibit tumor growth by combining PDT and chemotherapy. This innovative approach holds great promise as a powerful tool for cancer treatment, showing the potential to revolutionize PDT. Graphical abstract
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MOLECULAR NEUROBIOLOGY
·
2024
·
IF 4.6
Shen Yifen,Zhang Le,Yang Tao,Li Xiaosong,Liu Chao,Li Hongmei,Hu Yanping,Shen Hao,Li Hua,Orlov Yuriy L.,Zhou Shasha,Shen Yihang
Puberty onset through hypothalamic-pituitary-gonad (HPG) axis as an important reproductive event in postnatal development is initiated from hypothalamic arcuate nucleus (ARC). The growing evidence indicates that translational control also plays an essential role in the final expression of gonadotropin genes. To investigate the role of protein translation and behavior of ribosomes in pubertal onset, the global profiles of transcriptome, single ribosome (monosome), polysome, and tandem mass tag proteome were comprehensively investigated in rat hypothalamic ARCs of different pubertal stages using RNA sequencing, polyribo sequencing, and mass spectrum. Transcriptome-wide enrichments of N6-methyladenosine and IGF2BP2 were investigated using meRIP and RIP sequencing. Monosome was robustly enriched on a large proportion of mRNA in early puberty rats (postnatal day (PND)-25) compared to late puberty (PND-35 and PND-45). Monosome-enriched mRNAs, including HPG axis-related genes, had a large number of upstream ORFs (uORF, < 100 nt) and displayed translational repression in early puberty. Furthermore, insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) could particularly interact with and facilitate monosome to bind with mRNA in early puberty. Finally, ectopic over-expression of IGF2BP2 in hypothalamic ARC via lateral ventricle injection in vivo could recruit monosome to aggregate on mRNA and delay puberty onset. We uncovered a novel regulatory mechanism of IGF2BP2 and monosome for translational control in puberty onset, which shed light on the neuroendocrine regulatory network involved in HPG axis activation.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-17
·
IF 12.6
Guo Xueping,Li Huiyan,Li Zhihao,Cui Ziqi,Ma Guangming,Nassor Aisha Khalfan,Guan Yi,Pan Xiaohong
Background Environmentally responsive nanoscale biocide delivery system enhances smart, regulated, and synergistic biocide application with precise biocide release. In this study, pectin-modified dendritic mesoporous silica nanoparticles (DMSNs) was used as a carrier to successfully construct a microenvironment-responsive (pH, temperature and enzyme) eugenol nano-biocide delivery system for the control of Ralstonia solanacearum infection. Results The results showed that the specific surface area, pore size and surface activity of DMSNs significantly influence the biocide loading of eugenol, and the biocide loading capability was up to 72.50%. Eu@DMSNs/Pec had significant pH and pectinase stimulating effects, with varying release amounts under different temperature conditions. Compared with eugenol alone, Eu@DMSNs/Pec significantly enhanced the efficacy of eugenol. DMSNs assisted eugenol to induce peroxidation damage, produce ROS (•O 2 − , •OH and 1 O 2 ), achieve synergistic antibacterial effects, and had better rain erosion resistance and foliar retention rate based on pectin wettability and adhesion. Eu@DMSNs/Pec-FITC showed demonstrated efficient transport characteristics in tomato roots, stems and leaves, which enhanced the control effect on tomato bacterial wilt. In addition, Eu@DMSNs/Pec exert minimal influence on tomato seed germination and root growth, and have low toxicity to non-target organisms such as earthworms. Therefore, Eu@DMSNs/Pec environment-responsive nano-controlled release nanocarrier can effectively achieve accurate biocide release and reduce biocide dosage. Conclusion This work not only provides a pectin-modified DMSNs-based eugenol nanoscale biocide delivery system in response to specific environmental conditions of R. solanacearum infection but also elucidates the eugenol biocide loading, selective release ability and antibacterial mechanism of the system.
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RESPIRATORY RESEARCH
·
2025
·Vol. 26
·期 1
·1-15
·
IF 5
Wang Yudong,Liu Jun
Background Epithelial–mesenchymal transition (EMT) is regarded as a key process in repair of airway epithelium after injury. Forkhead Box C2 (FOXC2) is a transcription factor involved in EMT process, whether it is involved in repair of bronchial epithelium remains unknown.Methods C57BL/6 mice were subjected to intraperitoneal injection with naphthalene (NAPH; 200 mg/kg) to induce airway injury model. qPCR, immunoblot and FOXC2 immunohistochemistry assays were conducted to detect the expression of FOXC2 in bronchial epithelium. To explore the function of FOXC2 in NAPH-induced airway injury, the mice were given intratracheal administration of shFOXC2- or shNC-lentivirus particles, followed by NAPH treatment. Hematoxylin-and-eosin staining was used to assess the histopathology of the bronchial epithelium. Immunofluorescence analysis of CCSP, a club cell marker confirmed the CCSP expression in bronchial epithelium. Immunoblot and immunofluorescence assays determined the expression of E-cadherin, vimentin, and N-cadherin. In mouse primary bronchial epithelial cells (PBECs), we overexpressed and silenced FOXC2 by lentivirus particles, respectively. Cell migration was analyzed using wound healing assay. Immunoblot assays determined the E-cadherin, vimentin, FN-EDA expression in TGF-β1-induced PBECs. mRNA sequencing (mRNA-seq) and FOXC2 ChIP sequencing (ChIP-seq) to reveal the downstream genes of FOXC2 in TGF-β1-induced PBECs. Luciferase assay, ChIP-PCR and functional rescue experiments were performed to confirm the interaction of FOXC2/formin binding protein 1 (FNBP1) in TGF-β1-induced PBECs.Results FOXC2 expression was up-regulated in the lung tissues of mice at 2, 3 and 6 days post-NAPH. FOXC2 knockdown in bronchial epithelium of mice delayed CCSP+ club cell regeneration and normal repair of the airway epithelium within 14 days after injury. Knockdown of FOXC2 increased E-cadherin but decreased vimentin and N-cadherin, EMT markers during early phase after injury. In vitro, knockdown of endogenous FOXC2 repressed the migration of cells and increased TGF-β1-induced E-cadherin but decreased vimentin, N-cadherin and FN-EDA. Exogenous FOXC2 addition exerted opposite effects. Furthermore, mRNA-seq and FOXC2 ChIP-seq revealed that FNBP1 might be a downstream target of FOXC2. Overexpression of FNBP1 reversed the inhibitory role of FOXC2 knockdown in EMT.Conclusion sThese data highlight the important function of FOXC2 as a regulator in repair of bronchial epithelium after injury.Graphical
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-9
·
IF 5.3
Zhang Shengyuan,Yang Ying,Yang Dezhi,Yang Yaling
A metal–organic-framework (MOF) fluorescent sensor is reported based on NH 2 -MIL-101(Fe) propelled pesticide and alkaline phosphatase (ALP) catalytic reaction. Different from previous reports, a cascade reaction system combined with MOF structural changes to generate fluorescence was employed. The rationale is that ALP can hydrolyze L-ascorbic acid 2-phosphate (AAP) into L-ascorbic acid (AA), which can reduce Fe 3+ to decompose structurally NH 2 -MIL-101(Fe), resulting in 2-aminoterephthalic acid (NH 2 -BDC) with intense fluorescence. The fluorescence can be decreased to different degrees due to inhibition of organophosphate pesticides (OPPs) on the activity of ALP. By taking chlorpyrifos (CPY) as the model compound of an OPP pesticide and adding ALP and CPY into the NH 2 -MIL-101(Fe) framework, the resulting cascade reaction fluorescence sensors exhibit a good sensitivity for CPY and ALP sensing. The working ranges are 0.02–2 μg/L and 0.2–20 mU/mL with detection limits (LOD) of 5.31 ng/L and 0.05 mU/mL, respectively. The proposed sensor has been actually applied to satisfactory detection of CPY and ALP in food and serum samples. This fluorescence-based assay may extend the application of MOF-based biosensors. Graphical Abstract
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危险品化学品经营许可证（带存储）