NANOTECHNOLOGY
·
2021
·Vol. 32
·期 15
·155603
·
IF 3.874
Jun Dong,Yi Cao,Qingyan Han,Wei Gao,Tuo Li,Jianxia Qi
In this paper, Au nanoparticles (AuNPs) are prepared using wet chemical reduction transfer of dense AuNPs film by self-assembly to the surface of Ag grating, which is inverted from the inner DVD after evaporation. The Ag grating/AuNPs self-assembly hybrid substrate commonly used in surface-enhanced Raman scattering (SERS) research is produced. The coupling effect between AuNP-AuNP and AuNPs-Ag slugs can evidently enhance the local electric field. Experimental results show that the hybrid SERS substrate can detect 10−9 M Rh6G, and the enhancement factor reaches 4.4 × 105. This small, cheap hybrid substrate has enormous potential in the field of SERS sensing.
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RSC Advances
·
2021
·Vol. 11
·期 42
·25976-25982
·
IF 3.361
Fengyi Cao,Changmin Wei,Gangqing Ma,Like Hou,Rencong Zhang,Lin Mei,Qi Qin
Cotton gauze has been used as a wound dressing since the 19th century, and still plays an important role in current clinical therapies. However, the antimicrobial ability of cotton gauze is limited. In this work, gold nanoparticles (AuNPs) were used as photothermal transduction agents to synthesize modified photothermal antimicrobial cotton gauze. The modified cotton gauze was synthesized by immersing and heating the clinical cotton gauze with AuNPs solution. XPS, ICP-OES, FTIR, XRD and SEM characterizations confirmed that AuNPs were successfully decorated on the surface of cotton gauzes. Besides, the mechanical properties, air and water vapour permeability performance of cotton gauze were not changed after modification. Photothermal antimicrobial experiments confirmed that AuNPs modified on the cotton gauze could convert light to heat, inducing rapid temperature increase of the cotton gauze. And the heat could kill microbial cells permeated in the modified cotton gauze, giving it the potential of being used for photothermal antimicrobial therapy.
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CARBON
·
2019
·
IF 7.466
Yi Yu,QingYi Zhu,Feifan Xiang,Yue Hu,Lijie Zhang,Xiangju Xu,Nannan Liu,Shaoming Huang
AuNPs/SWCNT, a hybrid nanomaterial which is single-walled carbon nanotube (SWCNT) modified with gold nanoparticles (AuNPs), has great application prospects for chemical and biological sensors. For the innate nanogap between tiny AuNPs deposited on sidewall of SWCNT, AuNPs/SWCNT is suitable for the fabrication of simple and effective electrical signal switching devices. Here, we develop a novel strategy of applying AuNPs/SWCNT to fabricate nanogap device for DNA hybridization detection. The detection strategy is based on the target DNA mediating the catalytic activity of the AuNPs by desorbing the probe DNA from AuNPs. The signal transduction depends on the growth of tiny AuNPs which is closely related to the AuNPs catalytic activity. Sensing occurs at the tiny AuNPs growing and connecting into a continuous nanowire along the SWCNT, leading to a conductimetric response. The biosensing device can detect target DNA with the detection limit of 1 pM and the ability to discriminate triple and quintuple base pair mismatches.
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ACS Sensors
·
2019
·
IF 6.944
Xiaoqing Zhang,Ye Feng,Shaoyun Duan,Lingling Su,Jialin Zhang,Fengjiao He
The accurate and rapid detection of Mycobacterium tuberculosis (M. tuberculosis) is essential for the effective treatment of tuberculosis. In this article, we propose an electrochemical sensor to detect M. tuberculosis reference strain H37Rv. The sensor contains an H37Rv aptamer and oligonucleotides modified with gold nanoparticles (AuNPs–DNA). An H37Rv aptamer screened by our laboratory was used as the recognition probe. The change in frequency shift mediated by AuNPs–DNA in the presence of H37Rv was detected using a multichannel series piezoelectric quartz crystal (MSPQC) system. Three oligonucleotides modified with gold nanoparticles were designed. These oligonucleotides contained 12, 12, and 13 bases that hybridized with the 37-nt H37Rv aptamer. H37Rv aptamer was immobilized on the gold electrode surface by Au–S bonds. A conductive-layer was then formed by sequential hybridization of the aptamer with the three designed AuNPs–DNAs. When H37Rv was present, it specifically bound to the aptamer, resulting in the detachment of AuNPs–DNA from the electrode. The conductive layer was thereby replaced by a nonconductive complex of aptamer and bacteria. These changes were monitored by the MSPQC system. The proposed sensor is rapid, specific and sensitive, the detection time was 2 h. The detection limit was 100 cfu/mL. This sensor would be of great benefit for the early clinical diagnosis of tuberculosis.
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Journal of Biomedical Nanotechnology
·
2017
·Vol. 13
·期 10
·1253-1259
·
IF 4.521
Liu Yuan,Lai Yuxuan,Yang Gaojian,Tang Congli,Deng Yan,Li Song,Wang Zunliang
An ultrasensitive aptamer electrochemical biosensor based on glass carbon electrode (GCE) modified with chitosan (CS), gold nanoparticles (AuNPs) and aptamer has been successfully constructed and used to detect cadmium ions (Cd2+). The poly-(diallyl dimethyl ammonium chloride)
(PDDA) was applied to neutralize Cd-aptamer via electrostatic interaction. The presence of Cd2+ would trigger Cd-aptamer conformation change causing the Cd/Cd-aptamer complex to adsorb more electrochemical signal indicator [Ru(NH3)6]3+ instead of
PDDA. Through differential pulse voltammetry (DPV) detection, the peak current increased along with the increase in concentration of Cd2+, and the linear ranges were from 0.001 nM to 100 nM (1.124 × 10–13 g/mL to 1.124 × 10–8 g/mL) with
the detection limit as low as 0.04995 pM (5.614 ×10–15 g/mL). The proposed biosensor exhibited good stability, high sensitivity and selectivity towards Cd2+. Furthermore, this Cd-aptamer biosensor was demonstrated in testing Cd2+ in tap water samples
with satisfactory results.
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ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Juan Qiao,Hong Ding,Qianrong Liu,Rongyue Zhang,Li Qi
A microfluidic droplet synthesis approach for the preparation of poly N-isopropylacrylamide protected gold nanoparticles (PNIPAm@AuNPs) was presented here. Well-dispersed PNIPAm@AuNPs could be generated within 8 min. On the basis of the aggregation-induced UV–vis adsorption intensity increasing mechanism, the PNIPAm@AuNPs-based colorimetric probe displayed high sensitivity and good selectivity for sensing copper ions. A linear calibration of relative UV–vis adsorption intensity increasing versus copper ions concentration was obtained within 5.0–750.0 μM, and the limit of detection was 2.5 μM. Furthermore, after copper ions were injected in rat, a metabolic assay was developed with the proposed probe. The results indicated that the droplet microfluidic synthesis system could provide a new way for preparation of polymer@AuNPs with good polydispersity index and showed great potential of polymer@AuNPs-based sensing probe for application in biological and clinical analysis.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Meichen Ying,Gang Zhu,Wangdong Zhou,Liangqin Zhu,Jianfei Wang,Dexiang Feng,Yan Wei
A novel non-enzyme glucose sensor based on functionalized copper metal–organic framework composite material, AuNPs@MoS 2 /Cu-MOF, was developed for rapid glucose analysis. Cu-MOF nanomaterial was prepared via a hydrothermal method, followed by the surface loading of core–shell structured AuNPs@MoS 2 to synthesize AuNPs@MoS 2 /Cu-MOF composite material. Consequently, a novel gold nanoparticle @ molybdenum disulfide/copper metal–organic framework (AuNPs@MoS 2 /Cu-MOF/GCE) non-enzyme glucose sensor was constructed. Scanning electron microscopy (SEM), energy dispersive spectrometer (EDS) and X-ray powder Diffraction (XRD) was used to verify that the non-enzyme glucose sensor had been constructed effectively. Traditional electrochemical characterization methods were employed to study the electrocatalytic performance of the AuNPs@MoS 2 /Cu-MOF/GCE sensor and its catalytic oxidation of glucose. The experimental results demonstrated a good linear correlation between the concentration of glucose and the peak current within the range of 0.05–14.85 mM, with a detection limit of 16.7 μM. The AuNPs@MoS 2 /Cu-MOF/GCE sensor exhibited rapid electrochemical response to glucose (within 0.5 s), along with long-term stability and excellent selectivity. This is because Cu(II) in Cu-MOF can serve as active sites for the electrocatalytic oxidation of glucose, while the core–shell structure of AuNPs@MoS 2 with excellent conductivity can lower the energy barrier in the reaction, further accelerating this catalytic reaction. The AuNPs@MoS 2 /Cu-MOF/GCE sensor is cost-effective, easy to operate, and suitable for accurate rapid quantification of glucose.
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Analytical Methods
·
2024
·
IF 2.7
Xin Hua,Tongxiao Zhao,Xueqin Gui,Baokang Jin
In this paper, a label-free electrochemical immunosensor for sensitive detection of prostate antigen (PSA) was developed based on a NiFe PBA/AuNPs composite. The prostate antigen antibody was immobilized and the immunosensor was constructed by using a glassy carbon electrode modified with a nanocomposite consisting of nickel–iron Prussian blue analog (NiFe PBA) and gold nanoparticles (AuNPs). Due to the good biological affinity of AuNPs for biomolecules, as well as the porous nanostructure and regular shape of NiFe PBA, NiFe PBA/AuNPs nanocomposites significantly improve the electron transport rate, while achieving excellent performance for the sensor. Due to the interaction between the antibody and the antigen on the modified electrode, the current signal of the NiFe PBA itself is reduced due to the redox changes in Fe2+ and Fe3+, which can be determined by differential pulse voltammetry (DPV). Therefore, the monitoring of prostate antigen detection is realized. Under optimal experimental conditions, the immunosensor exhibited excellent detection performance with a dynamic response range from 0.5 pg mL−1 to 1000 pg mL−1 for the PSA concentration and a detection limit of 0.23 pg mL−1 (S/N = 3). In addition, the PSA aptasensor has good selectivity, high stability, and satisfactory reproducibility and has broad potential in clinical research and diagnostic applications.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Meizheng Li,Mengfei Luo,Jia An,Yufei Liu
Choline levels in the human body are indicative of diseases such as glioma, Alzheimer’s disease, and Parkinson’s disease, which is crucial in clinical diagnosis. Therefore, there is an urgent need for a method to detect choline that has potential clinical applications. A novel choline electrochemical biosensor based on choline oxidase was successfully developed, and its performance was significantly improved by modifying the composite of multi-walled carbon nanotubes and gold nanoparticles (MWCNT-AuNPs) and the composite of multi-walled carbon nanotubes and polyaniline (MWCNT-PANI). By combining the time–current method, the concentration of choline was determined based on the magnitude of the oxidation current, enabling trace-level detection of choline. The results showed that the modifying of nanomaterials on the electrode surface can improve both the conductivity and current response of the enzyme electrode. The choline oxidase/composite of multi-walled carbon nanotubes/composite of multi-walled carbon nanotubes and polyaniline and gold nanoparticles (ChOx/MWCNT-AuNPs/MWCNT-PANI) electrode showed a good linear response and a wide linear range to choline in the range of 0.033–133.330 μM. The results of the time–current indicated that our biosensor provided a higher sensitivity of 4.85 nA/μM and a lower detection limit of 0.025 μM (S/N = 3) for choline sensing. Furthermore, the sensor retained 90 % of the initial activity after 50 days. The results exhibited that the novel choline biosensor has the potential to be used for the clinical diagnosis of diseases and prevent disease recurrence, based on detecting the changes of choline level.
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TALANTA
·
2026
·
IF 6.7
Xinyi Wang,Minghao Wang,Wenbin Zhang,Dewang Yan,Liubing Kong,Gaofeng Wang
Accurate cortisol monitoring is essential for stress assessment and health management, yet reliable quantification remains challenging because signal fluctuations and matrix effects can compromise the performance of conventional single-mode immunosensors. Herein, we developed a fluorescence-colorimetric (FL-CL) dual-modal immunosensor based on a CuMOF-PEI@AuNPs@HRP (CPA) immunoreceptor for cortisol determination. The integrated sensing architecture generates correlated fluorescence and colorimetric responses from the same recognition event, which are combined through a normalized dual-signal output to improve calibration reliability. Under optimized conditions, the proposed sensor exhibited a detection limit of 0.22 ng/mL and a linear range of 3.1-200 ng/mL, covering physiologically relevant cortisol concentrations in saliva. Compared with single-modal assays and commercial kits, the proposed platform demonstrates enhanced sensitivity, improved correlation, good stability, and batch reproducibility. By integrating signal amplification, dual-modal readout, and simplified immunoreceptor fabrication within a single sensing platform, this work provides a reliable strategy for cortisol monitoring and offers potential for extension to other biomarker detection systems.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 12
·1-9
·
IF 6.408
Zhang Xiaoni,Peng Jun,Xi Liping,Lu Ziwei,Yu Lili,Liu Meiru,Huo Dezhi,He Hua
An elaborate composite of molecularly imprinted polymer (MIP)-modified gold nanoparticles (AuNPs)@silica dioxide (SiO 2 ) was designed and prepared for real-time colorimetric determination of glutathione (GSH) in serum. Firstly, the MIPs were synthesized on the surface of SiO 2 utilizing GSH as template molecules. Then, AuNPs were synthesized on the surface of MIPs@SiO 2 to produce a composite of MIPs modified by AuNPs@SiO 2 . Compared with plain AuNPs, the composite possessed better peroxidase catalysis activity due to stabilization and protection from hydrophilic SiO 2 , which can catalyze H 2 O 2 to·OH oxidizing 3,3,5,5-tetramethylbenzidine (TMB) to the colored product. In addition, its selectivity was enhanced by MIP modification with special recognition cavities. With the composite as the sensor, GSH was precisely and sensitively detected in the range 5 ~ 40 μM with a limit of determination of 1.16 μM according to the principle of inhibitive peroxidase catalysis activity by GSH. The proposed colorimetric detection was successfully utilized for selective, convenient, and rapid determination of GSH in serum. It provided a new strategy for drug real-time monitoring and has high potential in clinical drug analysis. Graphical Abstract
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FOOD CHEMISTRY
·
2020
·
IF 6.306
Lu Qiao,He Wang,Junlin He,Shuming Yang,Ailiang Chen
17β-estradiol (E2) residues could enrich in organisms via food chain and lead to harmful biological effects for human body. To ascertain the binding domain of original E2 aptamer (E00) with long-sequence (76-mer), we developed novel truncated aptamers from E00, through rationally designed truncation by intercepting a single ring or a combination of rings (containing hairpin loop, interior loop or multiloop) at different sites and retaining appropriate double helix regions. Through comparison, 15-mer E09 presented improved affinity and higher specificity, indicating the hairpin loop near to 3′ end of E00 served on the binding domain to E2. E09 was used for gold nanoparticles (AuNPs)-based colorimetric determination of E2, achieved the detection limit of 0.02 μg/mL. The truncated aptamer (only 15-mer) first proposed in this study has great application potential in E2 determination, and this work provides proof-of-concept study for truncation of other long-sequence aptamers.
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