INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Ping Shao,Haoya Zhang,Ben Niu,Ligang Jiang
It was previously found that R-(+)-Limonene emulsion could be stabilized by Ulva fasciata polysaccharide . In the present study, emulsions of R-(+)-Limonene were developed for coating of strawberries to improve the shelf life and microbiological safety. Edible coatings with at least 0.15% w/w of R-(+)-Limonene improved the microbial stability of the strawberries, resulted effective in the decontamination of external pathogens such as Escherichia coli , Bacillus subtilis and Staphylococcus aureus . Changes in weight loss, total soluble solid (TSS), pH, total phenolic, ascorbic acid , textural properties and surface color of strawberries during storage were evaluated. R-(+)-Limonene emulsions were effective for decreasing water loss of all the fruit samples tested. The decrease in TSS values was higher in the control strawberries. Initial decreases followed by increases of pH during storage of strawberries. The amount of total phenols detected in control fruit sharply increased during the first 6 days then remained steady, whereas the total soluble phenols content of treated strawberries gradually increased during storage. Strawberries coated with emulsion had higher L* and a* values as compared to control fruits. The R-(+)-Limonene coatings have demonstrated the potential to inhibit foodborne pathogen contamination of strawberries, and prolong their shelf life.
研究领域:
食品科学
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植物化学
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微生物学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Hui Huang,Chao Wang,Shuchang Liu,Zehao Sun,Dejun Zhang,Caicai Liu,Yang Jiang,Shuyue Zhan,Haofei Zhang,Ren Xu
The automatic identification (location, segmentation , and classification) by UAV- based optical imaging of spills of transparent floating Hazardous and Noxious Substances (HNS) benefits the on-site response to spill incidents, but it is also challenging. With a focus on the on-site optical imaging of HNS, this study explores the potential of single spectral imaging for HNS identification using the Faster R-CNN architecture. Images at 365 nm (narrow UV band), blue channel images (visible broadband of ∼400–600 nm), and RGB images of typical HNS (benzene, xylene, and palm oil) in different scenarios were studied with and without Faster R-CNN. Faster R-CNN was applied to locate and classify the HNS spills. The segmentation using Faster R-CNN-based methods and the original masking methods, including Otsu, Max entropy, and the local fuzzy thresholding method (LFTM), were investigated to explore the optimal wavelength and corresponding image processing method for the optical imaging of HNS. We also compared the classification and segmentation results of this study with our previously published studies on multispectral and whole spectral images. The results demonstrated that single spectral UV imaging at 365 nm combined with Faster R-CNN has great potential for the automatic identification of transparent HNS floating on the surface of the water. RGB images and images using Faster R-CNN in the blue channel are capable of HNS segmentation.
研究领域:
环境科学
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图像处理
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遥感技术
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产品关联
DOI
PubMed
高影响因子
FORENSIC SCIENCE INTERNATIONAL
·
2014
·
IF 2.115
Ting Wang,Baohua Shen,Yan Shi,Ping Xiang,Zhiguo Yu
Methamphetamine (MA) and amphetamine (AM) are widely abused drugs. Differentiation of MA and/or AM abuse from therapeutic ingestion of MA and/or AM or one of their precursor drugs is therefore of relevance in clinical and forensic toxicology. The aim of the study was to develop a simple, rapid, and accurate method for the chiral separation and determination of R / S -MA and R / S -AM in urine using liquid chromatography electrospray ionization tandem mass spectrometry operating in the positive ion multiple-reaction monitoring (MRM) mode. 20 μL of urine was diluted 500 times and 20 μL was injected. The chromatographic system consisted of a Chirobiotic™ V2 column (2.1 mm × 250 mm, 5 μm), and the mobile phase was methanol containing 0.1% (v/v) glacial acetic acid and 0.02% (v/v) ammonium hydroxide. The method was fully validated through assessments of its linearity (0.05–50.00 mg/L, r 2 > 0.994 for all analytes), and LOQ (0.05 mg/L for all analytes). No matrix effect was observed. The method was successfully applied to 86 urine samples from suspected MA abusers. Only the S -isomers of MA and AM were detected in 72 samples. The concentrations of R -MA ranged from below the LOQ to 13.76 mg/L in 14 urine samples with both enantiomers of MA and/or AM. Pure S -MA is the most common found analyte in urine and principally used by abusers.
研究领域:
分析化学
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法医学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2025
·
IF 5.1
Chang Zhang,Xin Zhang,Wei Wang,Hao Xue,Qian He,Fenglin Li,Xiaoli Wang,Qinghui Jin,Kun Zhou,Jiawen Jian
This paper presents an ammonia (NH3) fluorescent sensor based on the MAPbBr3 perovskite and the multifunctional organic material (R/S)-OBN-tCz. The incorporation of (R/S)-OBN-tCz enhances the sensor's photoluminescence (PL) intensity through Förster resonance energy transfer (FRET) and passivation effects, which consequently improves the sensitivity of the gas sensor. The MAPbBr3&(R/S)-OBN-tCz@PMMA nanofibers fabricated via electrospinning technology not only increase the specific surface area effectively, which is crucial for sensitivity improvement, but also strengthen the stability of the perovskite material under an air atmosphere. The experimental results demonstrate that the sensor maintains stable PL performance for up to 30 days in air and over 10 hours in water, while exhibiting rapid and reversible fluorescence response to NH3.
研究领域:
材料科学
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化学传感
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Feng Wang,Chao Li,Haohao Wang,Lu Yu,Fuming Zhang,Robert J. Linhardt
Natural amino acid polymers have been wide-used as bio-compatible emulsion stabilizers . In this study, Amphiphilic O(Phe- r -Glu)s were synthesized through the papain-catalyzed random polymerization of L -phenylalanine and L -glutamate with the major degree of polymerization value of 6–7. The polymerization yield was increased by more than 35 % using choline to reduce the energy barrier for the amino substrates to form the tetrahedral intermediate with the catalytic triad. The squalene/water emulsions stabilized by O(Phe- r -Glu) nanoparticles were pH-insensitive at pH 1–12. The investigation of mechanism found the combined effects of steric hindrance , electrostatic repulsion and the viscosity of the emulsion contributed to the stability of the emulsion droplets. Under acidic condition, the layers of O(Pho- r -Glu)s particle aggregates formed around the emulsion droplets and the higher viscosity of emulsion mainly contributed to the stability of emulsions . At basic condition, the strong electrostatic repulsion between emulsion droplets covered by the negatively charged O(Pho- r -Glu) particles and the steric hindrance contributed mainly to the prevention of the coalescence of emulsion droplets. The application of enzymatically synthesized amphiphilic peptides in emulsion, along with tunable performance through the polymerization of amino substrates, is in line with the sustainable development requirements in fields such as pharmaceuticals, food and cosmetics.
研究领域:
生物材料学
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食品科学
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制药工程
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产品关联
DOI
高影响因子
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2019
·Vol. 67
·期 3
·343-353
·
IF 1.559
Hai-Feng Hu,Hai-Yan Zhou,Gao-Ping Cheng,Ya-Ping Xue,Yuan-Shan Wang,Yu-Guo Zheng
R -2-(4-hydroxyphenoxy)propionicacid (HPOPA) is a valuable intermediate for the synthesis of enantiomerically pure aryloxyphenoxypropionic acid herbicides. In this work, to improve the HPOPA biosynthesis by Beauveria bassiana ZJB16002 from the substrate R -2-phenoxypropionic acid (POPA), the original HPOPA producer B. bassiana ZJB16002 was subjected to physical mutagenesis with 137 Cs-γ irradiation and chemical mutagen N -methyl- N' -nitro- N -nitrasoguanidine (NTG) induced mutagenesis. The effects of different treatment doses of the mutagens on the lethal rate and positive mutation rate were investigated, and the results showed that the optimal 137 Cs-γirradiation dose and NTG concentration was 850 Gy and 500 µg/mL, respectively. Under these conditions, a mutant strain CCN-7 with the highest HPOPA production capacity was obtained through two rounds of 137 Cs-γ irradiation treatment followed by one round of NTG mutagenesis. At the substrate (POPA) concentration of 50 g/L, HPOPA titer of CCN-7 reached 36.88 g/L, which was 9.73-fold higher than the parental strain. The morphology of the wild-type and mutant strain was compared and the results might provide helpful information in exploration of the correlation of morphology and biochemical features of B. bassiana .
研究领域:
微生物学
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生物工程
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 40
·19032-19042
·
IF 3.591
Chengmin Hou,Hanxiao Ma,Congjun Cao,Xiaojian Ding,Jingting Duan
The composite particles (St/Fe3O4) of starch and Fe3O4 were prepared by a co-precipitation method. Then AA and HEMA were grafted and cross-linked on St/Fe3O4 by a one-step method to obtain St/Fe3O4-g-p(AA-r-HEMA). The effects of the adsorbent dosage, pH value, initial Cu(II) concentration, time and temperature on adsorption were studied. Experiments showed that St/Fe3O4-g-p(AA-r-HEMA) showed a good adsorption performance for Cu(II), with a unit adsorption capacity of 75.5 mg g−1 and a maximum removal rate of 95.0%. The adsorption process followed the Langmuir model and the pseudo-second-order kinetic model, was dominated by chemical adsorption, and belonged to single-layer adsorption. Renewable performance studies showed that after 8 adsorption cycles, 91.2% of the first adsorption effect was still retained.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
ENERGY & FUELS
·
2024
·
IF 5.2
Xiaocao Cao,Yaowei Hao,Jiafan Zheng,Haodong Wang,Zhiguang Lin,Yafang Zhao,Jiayi Liu,Ming Zhang,Zhongrong Shen
Ruddlesden–Popper (R-P) perovskite oxides have attracted much attention as highly active and stable bifunctional materials for the oxygen evolution reaction (OER)/oxygen reduction reaction (ORR) in alkaline solutions due to the nonuse of precious metal elements. Herein, a triple (H+, O2–, and electron) conductive R-P perovskite oxide, La1.2Sr0.8Ni0.6Fe0.4O4+δ, was prepared, and the valence state of transition metal cations and highly oxidized oxygen (O–/O22–) in the structure was tuned by a low-temperature fluorine substitution treatment. The homogeneous distribution of the fluorine elements across the particles of the R-P perovskite oxide after its fluorination was confirmed by high-resolution transmission electron microscopy (HRTEM) images. By regulation of the amount of highly oxidative state oxygen species and the valence state of transition metal cations in the R-P perovskite structure, the material exhibits a significant enhancement for both the OER and ORR electrocatalytic activities. The fluoridated La1.2Sr0.8Ni0.6Fe0.4O4+δFy (LSNF-OF) achieves a low OER overpotential of 308.1 mV in a 1 M KOH electrolyte at a current density of 10 mA cm–2. This is superior to both commercial Co3O4 and the pristine sample without fluorination. The LSNF-OF electrode in an aqueous Zn-air battery (ZAB) exhibits a peak power density of 19.15 W g–1 at a current density of 24 mA g–1. The low-temperature trace fluorination can enhance the electrocatalytic efficiency of perovskite oxides. This technique can be applied to various types of metal oxides.
研究领域:
材料科学
·
电化学
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DOI
高影响因子
Systems Microbiology and Biomanufacturing
·
2022
·
IF 0
Zhu Jiufeng,Bai Yajun,Fan Tai-Ping,Zheng Xiaohui,Cai Yujie
Ethyl ( R )-4-chloro-3-hydroxybutyrate (( R )-CHBE), as a chiral intermediate, is widely used in the synthesis of various chiral drugs. In this study, we screened two aldo–keto reductases (LP-AKRs) from the probiotic Lactobacillus plantarum DSM20174, both with a molecular weight of approximately 31 kDa. Both enzymes could reduce 4-chloroacetoacetic acid ethyl ester (COBE) to produce ( R )-CHBE with an enantioselectivity value of 99%. When determining the kinetic parameter, the K m , K cat , and V max of LP-AKR5 and LP-AKR9 were 9.5 mM, 7.6 U/mg, 3.96 s −1 and 8.7 mM, 8.59 U/mg, 4.47 s −1 , respectively. Both LP-AKR5 and LP-AKR9 had an optimal reaction pH of 6 and could maintain a high level of stability at pH 6, allowing them to perform well in an acidic environment. LP-AKR5 and LP-AKR9 had optimal reaction temperatures of 30 °C and 40 °C, respectively. Metal ions had minimal influence on LP-AKR5 and LP-AKR9 enzyme activities. This series of enzymatic properties showed that LP-AKR5 and LP-AKR9 mined from Lactobacillus plantarum DSM20174 could asymmetrically catalyze the synthesis of ( R )-CHBE under weakly acidic circumstances, which could maintain product stability and provide a good foundation for industrial production.
研究领域:
生物化学
·
分子生物学
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工业微生物学
✓ 已验证
DOI
Separations
·
2025
·Vol. 12
·期 2
·22
·
IF 2.7
Huan-Huan Fan,Xiang-Ling Wang,Jie Feng,Wen-Ying Li
A comprehensive understanding of the compositions and physicochemical properties of coal-based liquids is conducive to the rapid development of multipurpose, high-performance, and high-value functional chemicals. However, because of their complex compositions, coal-based liquids generate two-dimensional gas chromatography (GC × GC) chromatograms that are very complex and very time consuming to analyze. Therefore, the development of a method for accurately and rapidly analyzing chromatograms is crucial for understanding the chemical compositions and structures of coal-based liquids, such as direct coal liquefaction (DCL) oils and coal tar. In this study, DCL oils were distilled and qualitatively analyzed using GC × GC chromatograms. A deep-learning (DL) model was used to identify spectral features in GC × GC chromatograms and predominantly categorize the corresponding DCL oils as aliphatic alkanes, cycloalkanes, mono-, bi-, tri-, and tetracyclic aromatics. Regional labels associated with areas in the GC × GC chromatograms were fed into the mask-region-based convolutional neural network’s (Mask R-CNN’s) algorithm. The Mask R-CNN accurately and rapidly segmented the GC × GC chromatograms into regions representing different compounds, thereby automatically qualitatively classifying the compounds according to their spots in the chromatograms. Results show that the Mask R-CNN model’s accuracy, precision, recall, F1 value, and Intersection over Union (IoU) value were 93.71%, 96.99%, 96.27%, 0.95, and 0.93, respectively. DL is effective for visually comparing GC × GC chromatograms to analyze the compositions of chemical mixtures, accelerating GC × GC chromatogram interpretation and compound characterization and facilitating comparisons of the chemical compositions of multiple coal-based liquids produced in the coal and petroleum industry. Applying DL to analyze chromatograms improves analysis efficiency and provides a new method for analyzing GC × GC chromatograms, which is important for fast and accurate analysis.
研究领域:
化学工程
·
人工智能
·
能源科学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED THERMAL ENGINEERING
·
2025
·
IF 6.9
Yanjun Sun,Haiqi Zheng,Bohao Yin,Xiaopo Wang,Maogang He
With global energy consumption surging and environmental concerns escalating, absorption refrigeration systems are receiving increasing attention. This study investigates the absorption behavior and thermodynamic performance of absorption refrigeration systems using trans -1-chloro-3,3,3-trifluoro-1-propene (R-1233zd(E)) paired with phosphonium-based ionic liquids (ILs), trihexyltetradecylphosphonium bis(trifluoromethylsulfonyl) amide ([P 66614 ][Tf 2 N]) and trihexyltetradecylphosphonium bis(2,4,4-trimethylpentyl) phosphinate ([P 66614 ][TMPP]). Experimental solubility measurements of R-1233zd(E) in the ILs were conducted using an isochoric saturation method across a temperature range of 293.15–343.15 K and pressures up to 0.61 MPa. The Non-Random Two-Liquid (NRTL) thermodynamic model was employed to correlate solubility data, yielding excellent agreement with deviations of less than 3.84 %. Results revealed that [P 66614 ][TMPP] exhibits higher solubility due to its branched alkyl chains, increased free volume, and favorable van der Waals interactions. Thermodynamic analyses were performed for single-effect (SARS), double-effect (DARS), and compression-assisted cycles (LC-SARS, single-effect cycle with a compressor added in the low-pressure side; HC-SARS, single-effect cycle with a compressor added in the high-pressure side; LC-DARS, double-effect cycle with a compressor added in the low-pressure side). It demonstrated that [P 66614 ][TMPP] exhibits a higher coefficient of performance (COP) compared to [P 66614 ][Tf 2 N]. Compression assistance reduced the minimum generation temperature and improved COP by leveraging electrical energy. Among all configurations, LC-DARS with a compression ratio of 1.9 demonstrated the optimal balance between COP (0.82) and thermal energy utilization efficiency. These findings highlight the potential of R-1233zd(E)/[P 66614 ][TMPP] as a sustainable working pair for absorption systems, particularly in low-grade heat recovery applications.
研究领域:
工程热物理学
·
化学工程
·
能源科学
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Genomics
·
2020
·
IF 2.414
Kejian Tian,Fanxing Meng,Qi Meng,Yan Gao,Lili Zhang,Le Wang,Yuqing Wang,Xue Li,Hongliang Huo
Estrogen contamination is recognized as one of the most serious environmental problems, causing widespread concern worldwide. Environmental estrogens are mainly derived from human and vertebrate excretion, drugs, and agricultural activities. The use of microorganisms is currently the most economical and effective method for biodegradation of environmental estrogens. Rhodococcus equi DSSKP-R-001 (R-001) has strong estrogen-degrading capabilities. Our study indicated that R-001 can use different types of estrogen as its sole carbon source for growth and metabolism, with final degradation rates above 90%. Transcriptome analysis showed that 720 (E1), 983 (E2), and 845 (EE2) genes were significantly upregulated in the estrogen-treated group compared with the control group, and 270 differentially expressed genes (DEGs) were upregulated across all treatment groups. These DEGs included ABC transporters; estrogen-degrading genes, including those that perform initial oxidation and dehydrogenation reactions and those that further degrade the resulting substrates into small molecules; and metabolism genes that complete the intracellular transformation and utilization of estrogen metabolites through biological processes such as amino acid metabolism, lipid metabolism, carbohydrate metabolism, and the tricarboxylic acid cycle. In summary, the biodegradation of estrogens is coordinated by a metabolic network of estrogen-degrading enzymes, transporters, metabolic enzymes, and other coenzymes. In this study, the metabolic mechanisms by which Rhodococcus equi R-001 degrades various estrogens were analyzed for the first time. A new pollutant metabolism system is outlined, providing a starting point for the construction of engineered estrogen-degrading bacteria.
研究领域:
微生物学
·
环境科学
·
基因组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）