Antibiotics-Basel
·
2025
·Vol. 15
·期 1
·
IF 4.6
Yongqing Liu,Huangwei Song,Muchen Zhang,Junyao Jiang,Yan Zhang,Jian Xu,Xi Xia,Shangshang Qin,Jianzhong Shen,Yang Wang
Background:The reintroduction of colistin has led to the rapid emergence of colistin-resistant strains, significantly diminishing its therapeutic efficacy. This presents a need for effective adjuvants to restore colistin efficacy.Approach: We screened the colistin adjuvants through a high-throughput method and then evaluated their synergistic effects and underlying mechanisms.Results: We identified β,β-dimethylacrylalkannin (β,β-Dim), a naphthoquinone compound derived from Lithospermum erythrorhizon, as a potent colistin adjuvant (fractional inhibitory concentration index (FICI) < 0.5). β,β-Dim enhanced colistin activity against 4 of 6 susceptible strains and all 18 colistin-resistant strains carrying either plasmid-bornemcrgenes (mcr-1,mcr-3,mcr-8, andmcr-9) or chromosomal two-component system (TCS) mutations (pmrA/B,phoP, andmgrB). These strains includedKlebsiella pneumoniae,Escherichia coli,SalmonellaTyphimurium,Pseudomonas aeruginosa, andAcinetobacter baumannii. The combination reduced the minimum inhibitory concentrations (MICs) of colistin by 4–1024-fold (from 512 to ≤2 µg/mL). Mechanistically, colistin-mediated outer membrane permeabilization facilitates β,β-Dim entry. Once internalized, β,β-Dim interacts with cytoplasmic membrane phospholipids and disrupts membrane biofunction. Further analysis showed that LPS transport and efflux pump activity were impaired, leading to LPS accumulation in the cytoplasmic membrane and increased intracellular colistin content. These processes elevated reactive oxygen species (ROS) production and markedly reduced ATP levels. In a murine infection model, β,β-Dim (2 mg/kg) combined with colistin (0.2 mg/kg) markedly increased survival from 20% (colistin alone) to 80%.Conclusions:These findings highlight that β,β-Dim combined with colistin is a promising therapeutic strategy for infections caused by colistin-resistant pathogens.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2018
·
IF 2.732
Siqi Fan,Xu Yao,Jingfang Li,Weizuo Li,Guangming Li
In view of the potential application of NIR luminescence in biology, a series of four new 3,5-Heptanedione (β-diketonate) ytterbium complexes, e.g. [Yb(L) 3 (H 2 O) 2 ] ( 1 ), [Yb(L) 3 (2,2′-bpy)] ( 2 ), [Yb(L) 3 (dpq)] ( 3 ) and [Yb(L) 3 (dppz)] ( 4 ) have been designed and isolated. Crystal structures analysis show that all complexes 1 – 4 exhibit the similar mononuclear structure, in which the central Yb 3+ ion is eight-coordinated forming the square-anti-prismatic geometry. NIR luminescence of all complexes 1 – 4 shows strong characteristic emission of Yb 3+ ions. To improve the mechanical and thermal stability, complex 1 was further doped into poly-(methyl methacrylate) (PMMA) for preparing a thin film ( 1 @PMMA). Their thermal stability and NIR luminescence were essentially enhanced in contrast to that for complex 1 . This NIR luminescent thin film may be of potential applications in biological dictation and luminescent probe.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xinyi Yin,Junhua Lu,Wenyu Du,Qiu Wu,Liying Han,Shupeng Su
For conventional emulsions used to encapsulate easily degradable bioactive compounds, achieving small droplet size and high encapsulation capacity is a challenging. Pickering emulsions stabilized by self-aggregated chitosan particles may offer high encapsulation efficiency due to the robust mechanical barrier formed by solid particles adsorbed at the oil-water interface. Therefore, the effects of pH, chitosan concentration, oil volume fraction, homogenization pressure, and homogenization cycle on the stability of chitosan Pickering emulsions and the degradation of β-carotene were investigated. Effective interfacial adsorption of chitosan nanoparticles and moderate homogenization intensity facilitated the formation of small emulsion droplets. Unlike conventional emulsions, chitosan Pickering emulsions with smaller droplets provided enhanced protection for β-carotene. This enhancement was primarily attributed to the improved interfacial coverage of chitosan nanoparticles with smaller droplet sizes, which was advantageous for β-carotene protection. The optimal conditions for preparing β-carotene-loaded chitosan Pickering emulsions were as follows: pH 6.5, chitosan concentration of 1.0 wt%, oil volume fraction of 20 %, homogenization pressure of 90 MPa, and 6 homogenization cycles. These findings indicate that chitosan Pickering emulsions are well-suited for encapsulating β-carotene with both small droplet size and high encapsulation efficiency.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2015
·
IF 3.65
Hailong Zhang,Xiaoyan Huang,Ya Sun,Guangyou Lu,Ke Wang,Zhigang Wang,Jianfeng Xing,Yang Gao
Effects of hydroxypropyl-β-cyclodextrin grafted polyethylenimine (HP-β-CD-PEI) including HP-β-CD-PEI 600 , HP-β-CD-PEI 1800 , HP-β-CD-PEI 10000 on the pulmonary absorption of insulin, calcitonin, 5(6)-carboxyfluorescein (CF) and fluorescein isothiocyanate dextrans (FDs) with various molecular weights (FD4, FD10 and FD70) were examined by an pulmonary absorption study in rats. Pulmonary absorptions of these poorly absorbable drugs were significantly enhanced by HP-β-CD-PEI 1800 and HP-β-CD-PEI 10000 , and HP-β-CD-PEI 1800 with the concentration of 5% (w/v) provided maximal absorption enhancing effect on pulmonary absorption of these model drugs. The toxicity study demonstrated that HP-β-CD-PEI did not induce any toxic action to rat pulmonary membranes. In addition, zeta potential of insulin solution changed to positive by addition of various HP-β-CD-PEI, meanwhile, the degree of positive charge was linearly correlated with absorption enhancing effect of HP-β-CD-PEI, suggesting that positive charge of HP-β-CD-PEI might be related to their absorption enhancing mechanisms for enhancing pulmonary absorption of insulin in rats. In conclusion, HP-β-CD-PEI is a potential and safe absorption enhancer for improving absorption of hydrophilic macromolecules especially peptide and protein drugs by pulmonary delivery.
研究领域:
药理学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD BIOTECHNOLOGY
·
2016
·
IF 0.814
Ming-Ming Zhu,Shu-Lin Wang,Ming-Tao Fan
This study was designed to isolate and identify a novel β-carotene cleaving microorganism from sea buckthorn juice and to detect the carotenoid-derived aroma compounds catalyzed by the microbial enzyme. The strain was characterized based on morphological and biochemical properties, as well as part of its 16S rDNA sequence analysis. It was then identified using Basic Local Alignment Search Tool (BLAST) and classified as coagulase-negative Staphylococcus pasteuri (GeneBank Accession No. KP171185). β-Ionone and β-cyclocitral were formed as the main flavor products of β-carotene in submerged culture. When cell-free culture supernatant was applied for the conversion, nearly complete degradation of β-carotene (2 mg) was observed after 12 h. β-Ionone and β-cyclocitral were the main aromas, whereas β-ionone-5,6-epoxide, 2-hydroxy-2,6,6-trimethyl cyclohexanone and dihydroactinidiolide were formed in minor quantities. Moreover, the carotenoid-cleavage enzyme activities of S. pasteuri were partially characterized and partially purified enzyme possesses high activity in adverse industrial condition.
研究领域:
微生物学
·
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2014
·Vol. 28
·期 8
·635-640
·
IF 2.017
Xiaohua Zhao,Xiang Liu,Ming Lu
Low-melting β-cyclodextrin/ N -methylurea (NMU) mixture, an efficient catalytic system for ligand-free Suzuki and Heck couplings in the presence of fresh native β-CD-capped Pd 0 nanoparticles, has been successfully reported. This natural and convenient system can be performed in air and could afford the corresponding cross-coupled products in good to excellent isolated yields after a simple workup under every low Pd loading (0.05 mol%). Remarkably, the catalytic system can be recycled and reused without loss of catalytic activity. Copyright © 2014 John Wiley & Sons, Ltd.
研究领域:
化学
·
材料科学
·
催化反应
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Zhisheng Zhou,Miao Zhu,Guowen Zhang,Xing Hu,Junhui Pan
5−Hydroxymethyl−2−furaldehyde (5-HMF) in dairy food products generated via Maillard reaction may bring health risks. Herein, the interaction of 5-HMF with β-casein (β-CN) and its influences on the structure and function of β-CN were investigated. 5-HMF was found to quench the inherent fluorescence of β-CN by a static mode and the binding constant was in the order of magnitude of 10 4 L mol −1 . Thermodynamic analysis indicated that hydrophobic forces played the major role in the interaction. The results of synchronous and three-dimensional fluorescence revealed that 5-HMF changed the microenvironment of tyrosine and tryptophan residues and interfered the stability of the polypeptide backbone structure in β-CN. Molecular docking revealed that 5-HMF bound to the hydrophobic cavity of β-CN mainly interacting with the amino acid residues Pro196, Val155, Leu163, Phe157 and Leu192. The binding of 5-HMF with β-CN caused partial unfolding of β-CN conformation, resulting in the increase in surface hydrophobicity and emulsifiability, and decrease in emulsification stability of β-CN, thereby reducing the solubility of β-CN. The study might facilitate the comprehensive understanding of 5-HMF affecting the structure and functional properties of β-CN.
研究领域:
食品科学
·
生物化学
·
分子生物学
✓ 已验证
DOI
高影响因子
MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2018
·Vol. 219
·期 18
·1800189
·
IF 2.492
Meina Xiao,Jingxing Jiang,Tongtong Zhang,Anli Xu,Zhuofeng Ke,Liangzhi Hong
The hybrid copolymerization of vinyl monomers with cyclic esters endows the formed copolymers new features, such as biodegradability and functionality. However, the recently reported hybrid copolymerizations are mainly based on polar vinyl monomers and cyclic esters catalyzed by organocatalysts. The copolymerization of non-polar vinyl monomers with cyclic esters is still a challenge. In this work, the copolymerization of β-butyrolactone (β-BL) with styrene catalyzed by B(C 6 F 5 ) 3 in the absence of coinitiators is reported. The density functional theory studies suggest the formation of zwitterion between β-BL and B(C 6 F 5 ) 3 . It is found that β-BL and styrene are concurrently copolymerized through the macro-zwitterion intermediates. The obtained copolymers are carefully examined by 1 H NMR, 2D 1 H- 13 C HMBC, differential scanning calorimetry, thermogravimetric analysis, hydrolysis experiments, as well as Brady's reagents.
研究领域:
高分子化学
·
有机化学
✓ 已验证
DOI
高影响因子
THERMOCHIMICA ACTA
·
2013
·
IF 1.989
Haisu Gao,Liping Chen,Wanghua Chen,Shilong Bao
The thermal stability of β-artemether under dynamic, isothermal and adiabatic conditions were investigated by differential scanning calorimeter (DSC) and accelerating rate calorimeter (ARC), respectively. The dynamic DSC measurements at various heating rates (1, 2, 4 and 8 °C min −1 ) displayed that β-artemether experienced polymorph transformation in the melting process. According to the isothermal DSC results at different temperatures 86, 88, 90 and 92 °C, β-artemether decomposed after the completion of the melting and the polymorph transformation, and the thermal decomposition of β-artemether is hazardous for its characteristic of autocatalytic decomposition. The kinetics analysis dynamically and isothermally described by Friedman method indicated the thermal decomposition of β-artemether did not comply with a single mechanism. The ARC results showed that the pressure increased with the increase of temperature, and indicated obvious linear relationship. Based on the ARC data, the value of SADT of β-artemether in 50 kg package was obtained.
研究领域:
药学
·
化学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Shuanghe Li,Sang Luo,Hui Wang,Hui Liu,Jiayue Liu,Xu Zhang,Bingren Tian
Fabricating food packaging films with multifunction (antibacterial, antioxidant, and controlled-release properties) represents a powerful strategy for extending the shelf life and enhancing the safety of perishable fruits. Herein, chitosan (CS) and polyvinyl alcohol (PVA) composite films were loaded inclusion complex (IC) with different concentrations of β-acids/β-cyclodextrin (β-acids/β-CD) or β-acids/hydroxyethyl-β-cyclodextrin (β-acids/HE-β-CD) inclusion complex. Moreover, the films' physical, chemical, and biological properties were systematically investigated. Satisfyingly, these composite films exhibited excellent mechanical properties and thermal stability. As the concentration of the inclusion complex improved, the hydrophobicity of the functionalized films also enhanced (76.50°–108.59°/110.04°). Furthermore, variations in pH were discovered that it could affect β-acids release from films. A significant enhancement in the antioxidant capacity of films could be clearly observed with increased concentrations of the inclusion complex (from 5.56 % to 72.98 %/73.47 %). Additionally, films expressed outstanding antibacterial activity against Gram-positive bacteria ( S. aureus ) (inhibition zone >10.89 mm), with those loading β-acids/β-CD demonstrating a stronger antibacterial effect than those loading β-acids/HE-β-CD. For the preservation of strawberries – the typical representative of perishable fruits, the films significantly extended their shelf life to eight days. As the content of the inclusion complex of β-acids in the film increased, the weight loss dramatically reduced (39.91 % without film vs. 23.12 % with HE-β-CD@CS/PVA film). Overall, the CS/PVA films loading β-acids/β-CD display remarkable multifunctionality and value as a fresh-preserving packaging material with sustained release performance and notable bioactivity.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chem Catalysis
·
2025
·
IF 11.6
Yunfeng Bao,Jingzhen Zhang,Shiwen Du,Sanlue Hu,Yejun Xiao,Zhaochi Feng,Fuxiang Zhang
Inorganic mixed-anion semiconductor photocatalysts are promising for water splitting due to their broad visible light absorption and structural stability. However, efficient photocatalytic production of hydrogen peroxide (H 2 O 2 ) remains challenging. This study reports a one-step photocatalytic method for H 2 O 2 production using the synthesized β-ZrNBr inorganic nanosheet photocatalyst, achieving an apparent quantum efficiency of 11.7% at 420 ± 10 nm and a solar-to-H 2 O 2 efficiency of 0.5%. This superior H 2 O 2 synthesis performance is attributed to efficient carrier mobility, high bulk charge carrier separation efficiency exceeding 70%, and the ability to directly promote water oxidation into H 2 O 2 . Additionally, 600 μmol L −1 g cat −1 of H 2 O 2 can be continuously generated over the β-ZrNBr film without significant decay under visible light in a homemade flow cell. Our work offers a scalable alternative for H 2 O 2 production using robust inorganic semiconductors under ambient conditions.
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
DOI
高影响因子
Advances in Applied Ceramics
·
2025
·
IF 1.6
Maohui Li,Mingjun Zhang,Bo Ma,Wenzhou Sun,Yanmin Wang,Youjun Lu
The brittleness of silicon carbide restricts its wide application. Graphene as a superior second-phase material can improve the toughness and brittleness of silicon carbide. In this article, a graphene/β-SiC ceramic material was prepared with 1–3 wt.% graphene added in β-SiC matrix via pressure-free sintering at 2120°C for 60 min. The effect of the graphene addition on the phase composition, bulk density, porosity, and mechanical properties of ceramic material was investigated. The results show that the bulk density of graphene/β-SiC ceramic material decreases, the porosity of openings increases, and the bending strength, hardness, as well as fracture toughness firstly increase and then decrease as graphene addition increases. Compared with β-SiC ceramic without graphene, the optimum properties of graphene/β-SiC ceramic with graphene addition of 1 wt.% (i.e. a fracture toughness of 5.07 MPa m1/2, bending strength of 410.84 MPa and hardness of 27.72 HV) can be obtained, which are increased by 35.56%, 13.35%, and 3.8%, respectively. The fracture morphology indicates that the crack deflection is the main mechanism of toughening β-SiC ceramic with graphene.
研究领域:
材料科学
·
陶瓷工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）