BIOELECTROCHEMISTRY
·
2018
·
IF 3.789
Qiang Yuan,Y. Huang,Debao Liu,Minfang Chen
Biodegradable beta-tricalcium phosphate (β-TCP) particle reinforced magnesium metal matrix composites (Mg-MMC) have attracted increasing interest for application as implant materials . This investigation was conducted to study the effect of cooling rate on the microstructure and corrosion behavior of a biodegradable β-TCP/Mg-Zn-Ca composite. The composite was fabricated under a series of cooling rates using a wedge-shaped casting mold. The microstructure of the composite was examined by optical and scanning electron microscopy, and the corrosion behavior was investigated using an electrochemical workstation and immersion tests in a simulated body fluid (SBF). Faster cooling rates were shown to refine the secondary phase and grain size, and produce a more homogenous microstructure. The refined microstructure resulted in a more uniform distribution of β-TCP particles, which is believed to be beneficial in the formation of a stable and compact corrosion product layer, leading to improved corrosion resistance for the composite.
研究领域:
材料科学
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生物医学工程
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腐蚀科学
·
金属基复合材料
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生物材料
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DOI
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 20
·3279-3290
·
IF 5.047
Shuang Yu,Jun Shi,Yutong Liu,Jiawen Si,Yuan Yuan,Changsheng Liu
The treatment of bone defects in the field of periodontology and oral implantology has been a major clinical problem to date. Guided bone regeneration (GBR), based on ideal barrier membranes to provide space maintenance and selectively promote the regeneration of the damaged area by prevention of invasion by the fibrous tissues, represents the most promising strategy at present. In this study, the mechanically robust and flexible membrane PEGylated poly(glycerol sebacate) (PEGS) coordinated by β-TCP nanoparticles (PEGS/β-TCP) was developed by a simple prepolymer mixing-in situ crosslinking method. By tailoring the content of β-TCP (0, 10, 50, and 90 wt% relative to the prepolymer), the resultant PEGS/β-TCP composite membranes exhibited controllable degradation rate and reinforced mechanical properties. The maximum tensile strength of 9.58 ± 0.02 MPa was presented by the P20T50 group (weight ratio of β-TCP to PEGS20 prepolymer was 50%), about 1.5-fold higher than that of P20T0 without β-TCP. For cellular responses, the PEGS/β-TCP membranes showed desirable cell attachment and viability on rat bone mesenchymal stem cells (rBMSCs). The incorporation of β-TCP definitely enhanced the alkaline phosphatase activity and promoted mineralization, thus facilitating the osteogenic differentiation. The in vivo result obtained for the rat calvarial defect model reaffirmed the favorable bone regenerative ability of the fabricated membranes, especially for P20T50 with the highest bone volume/tissue volume ratio (BV/TV) at both 4 weeks (17.26 ± 1.49%) and 8 weeks (23.24 ± 2.85%) after the surgery. Therefore, the PEGS/β-TCP composite membranes prepared by this prepolymer mixing-in situ crosslinking process will be a prospective biomaterial for the GBR therapy.
研究领域:
生物材料学
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口腔医学
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组织工程学
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DOI
高影响因子
CRYSTENGCOMM
·
2018
·Vol. 21
·期 3
·513-523
·
IF 3.304
Xiao Liu,Naru Zhao,Xiaoheng Guo,Haibo Duan,Jingjing Diao,Yifan Dong,Yingjun Wang
Cellular behavior is a result of homologous responses to biomaterials, especially material surfaces. An effective way to elicit a desired biological response is to employ micro/nanostructured constructions for biomaterial surface design; however, the brittleness of inorganic materials usually hinders structure regulation. In this study, a micro/nanostructured surface was fabricated using a hydrothermal treatment that did not require crystal regulation reagents. The precursor, β-tricalcium phosphate, was mixed with CaSiO3. The resulting micro/nanostructured surface had a hybrid structure containing nanowires, microrods, and rod clusters. In vitro cellular proliferation, cytotoxicity and osteodifferentiation experiments indicated that the micro/nanostructured surfaces alleviated material cytotoxicity, improved cell proliferation, and effectively promoted osteogenic differentiation of mouse bone mesenchymal stem cells. These results suggest that this simplified surface treatment is an efficient way to improve cell biological response to inorganic biomaterials.
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产品关联
DOI
高影响因子
FASEB JOURNAL
·
2023
·Vol. 37
·期 5
·e22924
·
IF 4.8
Liangjie Lu,Huihan Wang,Minjie Yang,Lijun Wang,Kaifeng Gan
Beta-tricalcium phosphate (β-TCP) is considered as one of the most promising biomaterials for bone reconstruction. This study generated a functional molybdenum disulfide (MoS 2 )/polydopamine (PDA)/−bone morphogenetic protein 2 (BMP2)-insulin-like growth factor-1 (IGF-1) coating on the β-TCP scaffold and analyzed the outcomes. The MoS 2 /PDA-BMP2-IGF-1@β-TCP (MPBI@β-TCP) scaffold was prepared by 3D printing and physical adsorption, followed by characterization to validate its successful construction. The in vitro osteogenic effect of the MPBI@β-TCP scaffold was evaluated. It was found that MPBI@β-TCP augmented the adhesion, diffusion and proliferation of mesenchymal stem cells (MSCs). The alkaline phosphatase (ALP) activity, collagen secretion and extracellular matrix (ECM) mineralization along with the expression of Runx2, ALP and OCN were also enhanced in the presence of MPBI@β-TCP. Additionally, MPBI@β-TCP stimulated endothelial cells to secrete VEGF and promoted capillary-like tubule formation. We then confirmed the biocompatibility of MPBI@β-TCP to macrophages and its anti-inflammatory effects. Furthermore, under near-infrared (NIR) laser irradiation, MPBI@β-TCP produced photothermal effect to not only kill MG-63 osteosarcoma cells, but also enhance bone regeneration in vivo with biosafety. Overall, this work demonstrates that 3D-printed MPBI@β-TCP with enhanced osteogenic activity under NIR laser irradiation has a vast potential in the field of tissue defects.
研究领域:
生物医学工程
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材料科学
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组织工程
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肿瘤学
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再生医学
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产品关联
DOI
PubMed
高影响因子
Biomaterials Advances
·
2022
·
IF 0
Dahu Qi,Jin Su,Song Li,Hao Zhu,Lijin Cheng,Shuaibin Hua,Xi Yuan,Jiawei Jiang,Zixing Shu,Yusheng Shi,Jun Xiao
Bioceramics have been used in orthopedic surgery for several years. Magnesium (Mg) is an essential element in bone tissue and plays an important role in bone metabolism. Mg-doped bioceramics has attracted the attention of researchers recently. However, the optimal doping amount of Mg in β-TCP and the immunomodulatory property of Mg-doped β-TCP (Mg-TCP) have not been determined yet. In this study, β-TCP scaffolds doped with different contents of magnesium oxide (0 wt%, 1 wt%, 3 wt%, and 5 wt%) with gyroid structure were printed by digital light processing (DLP) method, and the physicochemical and biological functions were then investigated. Mg-doping improved the physicochemical properties of the β-TCP scaffolds. In vitro experiments confirmed that the doping of Mg in β-TCP scaffolds promoted the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and angiogenic differentiation of endothelial progenitor cells (EPCs), where the 5Mg-TCP has the optimal properties when using the “one cell type” method. It was also found that all Mg-TCP facilitated the polarization of RAW264.7 cells to the M2 phenotype, especially the 3Mg-TCP. However, 3Mg-TCP displayed the optimal osteogenic and angiogenic potential when using a “multiple cell type” method, which referred to culturing the BMSCs or EPCs in the macrophage-conditioned medium. Finally, the in vivo experiments were conducted and the results confirmed that the 3Mg-TCP scaffolds possessed the satisfying bone defect repair capability both after 6 and 12 weeks of implantation. This study suggests that 3Mg-TCP scaffolds provide the optimal biological performance and thus have the potential for clinical translation.
研究领域:
骨科手术
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生物材料
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组织工程
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生物医学工程
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DOI
PubMed
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2021
·Vol. 32
·期 12
·1-13
·
IF 3.896
Xu Wanghan,Tan Wei,Li Chan,Wu Keke,Zeng Xinyi,Xiao Liwei
Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment. It is imperative for us to develop a therapeutic scaffold to repair the alveolar bone defect of periodontitis. In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15). The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET. The MET-loaded β-TCP/CTS/SBA-15 scaffolds showed improved cell adhesion, appropriate porosity and good biocompatibility in vitro. This MET composite scaffold was implanted in the alveolar bone defects area of rats with periodontitis. After 12 weeks, Micro-CT and histological analysis were performed to evaluate different degrees of healing and mineralization. Results showed that the MET-loaded β-TCP/CTS/SBA-15 scaffolds promoted alveolar bone regeneration in a rat model of periodontitis. To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis. Our findings might provide a new and promising strategy for repairing alveolar bone defects under the condition of periodontitis.
研究领域:
牙科材料
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组织工程
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生物材料
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口腔生物学
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再生医学
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DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Yu Hu Zhou,Zi Hao Zou,Jian Xiang Teng,Zhan Yu Wu,Si Wei Luo,Xu Ning,Chuan Ye,Long Yang,Wei Seong Toh
Avascular necrosis of the femoral head (ANFH) is a debilitating musculoskeletal disorder that is typically caused by impaired blood supply to the hip joint. In treating irregular bone defects that resulted from ANFH, injectable hydrogels are particularly attractive as they can be administered in a minimally invasive manner and conform to the variable shape of a bone defect. However, they often lack the biochemical and mechanical properties for effective bone repair. To address these issues, an injectable composite hydrogel, SA/β-TCP@PMP, composed of sodium alginate (SA) and β-tricalcium phosphate (β-TCP) crosslinked with glucono-delta-lactone (GDL), and enforced with varying concentrations of poly(3-hydroxybutyrate- co -4-hydroxybutyrate)/magnesium oxide and polyethylene glycol (P34HB/MgO+PEG; PMP) coaxial electrospun microfibers are formulated. By varying the concentrations (0%, 1%, 2%, 4%) of PMP microfibers, the physical properties of the composite hydrogel, including its injectability, surface morphology, swelling, degradation, and mechanical properties, as well as magnesium ions (Mg 2+ ) release can be finely tuned. Among all, SA/β-TCP@2%PMP composite hydrogel demonstrates superior angiogenic and osteogenic properties, and promotes robust new bone formation in a rabbit model of steroid-induced ANFH. Overall, this novel composite hydrogel offers significant application potential for minimally invasive treatment of irregular bone lesions in ANFH.
研究领域:
生物医学工程
·
骨科
·
材料科学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Zhen Zhang,Geng Chen,Jun Wang,Xin Hu,Peijie Hou,Chengdong Xiong,Lifang Zhang
Addressing the prevalent clinical issue of bone defects, this study successfully developed a novel ternary copolymer material (PLCD) based on polylactic acid (PLA), polycaprolactone (PCL), and polydioxanone (PPDO). This ternary material combines the strength of PLA, the toughness of PCL, and the controllable rapid degradation properties of PPDO. making it a promising candidate for biomedical applications. By using an optimized emulsion method combined with finite element analysis, microspheres with a particle size range of 500–700 μm were prepared and subsequently fabricated into microsphere scaffolds using the solvent sintering method. To enhance osteoinductive and osseointegration capabilities, various proportions of β-tricalcium phosphate (β-TCP) were uniformly dispersed within the microspheres, creating composite scaffolds. Additionally, nano zinc oxide were coated on the microsphere scaffold surface using the in situ polymerization properties of dopamine, imparting antibacterial properties and enhancing functionality. Physical and biological evaluations of the microsphere scaffolds revealed excellent overall performance, including an interconnected porous structure, porosity and mechanical properties matching those of cancellous bone, suitable degradation behavior, and in vitro mineralization ability. Furthermore, scaffolds coated with polydopamine and loaded with nano zinc oxide exhibited strong initial antibacterial effects, and promoted osteogenic differentiation. These results indicate that the developed composite microsphere scaffolds have significant potential in bone tissue engineering applications, offering a promising solution for effective bone regeneration.
研究领域:
生物材料学
·
医学工程
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产品关联
DOI
高影响因子
Journal of the Mechanical Behavior of Biomedical Materials
·
2024
·
IF 3.3
Chao Yao,Prisana Pripatnanont,Junbiao Zhang,Srisurang Suttapreyasri
One strategy to correct alveolar bone defects is use of bioactive bone substitutes to maintain the structure of defect site and facilitate cells and vessels' ingrowth. This study aimed to fabricate and characterize the freeze-dried bone regeneration scaffolds composed of polymeric Type I collagen, nano Beta-tricalcium phosphate (β-TCP), and gelatin. The stable structures of scaffolds were obtained by thermal crosslinking and EDC/NHS ((1-ethyl-3-(3-dimethylaminopropyl) carbodiimide)/(N-hydroxysuccinimide)) chemical crosslinking processes. Subsequently, the physicochemical and biological properties of the scaffolds were characterized and assessed. The results indicated the bioactive composite scaffolds containing 10% and 20% (w/v) nano β-TCP exhibited suitable porosity (84.45 ± 25.43 nm, and 94.51 ± 14.69 nm respectively), a rapid swelling property (reaching the maximum swelling rate at 1 h), excellent degradation resistance (residual mass percentage of scaffolds higher than 80% on day 90 in PBS and Type I collagenase solution respectively), and sustained calcium release capabilities. Moreover, they displayed outstanding biological properties, including superior cell viability, cell adhesion, and cell proliferation. Additionally, the scaffolds containing 10% and 20% (w/v) nano β-TCP could promote the osteogenic differentiation of MC3T3-E1. Therefore, the bioactive composite scaffolds containing 10% and 20% (w/v) nano β-TCP could be further studied for being used to treat alveolar bone defects in vivo.
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DOI
PubMed
高影响因子
Materials
·
2023
·Vol. 16
·期 3
·947
·
IF 3.4
Yanlong Wu,Ruomeng Chen,Xu Chen,Yongqiang Yang,Jian Qiao,Yaxiong Liu
Strong and tough β-TCP/PCL composite scaffolds with interconnected porosity were developed by combining digital light processing and vacuum infiltration. The composite scaffolds were comprised of pure β-TCP, β-TCP matrix composite and PCL matrix composite. The porous β-TCP/PCL composite scaffolds showed remarkable mechanical advantages compared with ceramic scaffolds with the same macroscopic pore structure (dense scaffolds). The composite scaffolds exhibited a significant increase in strain energy density and fracture energy density, though with similar compressive and flexural strengths. Moreover, the composite scaffolds had a much higher Weibull modulus and longer fatigue life than the dense scaffolds. It was revealed that the composite scaffolds with interconnected porosity possess comprehensive mechanical properties (high strength, excellent toughness, significant reliability and fatigue resistance), which suggests that they could replace the pure ceramic scaffolds for degradable bone substitutes, especially in complex stress environments.Keywords:porous composite scaffolds;mechanical properties;digital light processing;infiltration;gradient structure
研究领域:
生物材料学
·
骨科工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2025
·
IF 14.3
Kang Wang,Zexin Liu,Lin Liu,Tiantian Wang,Kun Chen,Runhua Yao,Ruiqiang Hang,Xiaohong Yao
Zinc-based materials have been extensively researched as biodegradable bone repair materials in recent years, but their low degradation rate and limited bioactivity need to be further improved. In this study, a new-type dual-sized macro-micro porous Zinc-Magnesium-β-tricalcium phosphate (DMMP Zn-Mg-β-TCP) scaffolds were designed and fabricated by vacuum heating-press sintering. The mechanical properties, degradation behaviors, in vitro biocompatibility, in vivo osteogenesis, and biological safety of the scaffolds were systematically evaluated. The results show that DMMP Zn-Mg-β-TCP has suitable mechanical properties, degradation properties, and excellent osteogenic properties. The compressive yield strength range of the scaffolds is 12.90–25.46 MPa, and the elastic modulus range is 0.31–0.44 GPa, meeting the mechanical properties required for implant materials in cancellous bone. In vitro degradation tests showed that the corrosion rate of the scaffolds ranges from 2.25 to 2.61 mm/y. Furthermore, the scaffolds exhibited excellent in vitro cell compatibility. After a 24-week animal experiment, the scaffold demonstrated excellent osteogenic capacity during bone healing, while maintaining biomechanical stability throughout its degradation process. In summary, the DMMP Zn-Mg-β-TCP has the potential to become a novel biodegradable bone implant material.
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产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2021
·
IF 4.527
ZhiDong Lin,XuLin Hu,LiLun Zhong,DeQiang Peng,JunLin Lu,Jian He,Xiaofei Shen,ChengDong Xiong,Tao Xu,Wei Niu
β tricalcium phosphate (β-TCP) is a common biological ceramic in bone tissue engineering due to its excellent biocompatibility and biodegradability. However, owing to its inherent properties of poor injectability and forming ability, the application of TCP in extrusion 3D printing is quite limited. To solve this problem, we innovatively printed gelatin and PVA (Polyvinyl alcohol) binary systems by taking advantage of the ability of gelatin to solidify rapidly at low temperatures and the ability of PVA to effectively improve phase separation during extrusion. Here, we fabricated a series of novel Gel-PVA-TCP scaffolds by incorporating different concentrations of PVA (5%, 10%, 15%) through 3D printing. Collectively, the addition of PVA made differences in three ways. First, compared to the PVA-free system, the novel printing system significantly improved the printability of TCP paste. The printing temperature was decreased to 35–45 °C by doping different concentrations of PVA. Next, the novel printing system maintained the excellent forming ability and printing precision of the scaffolds. Compared to the macropores of scaffolds without doping PVA (456.1 ± 11.2 μm), the macropores of Gel-PVA-TCP were 486.1 ± 26.5, 446.1 ± 15.2, and 443.7 ± 26.3 μm. Last, after sintering, the scaffolds with different concentrations of PVA exhibited similar compressive strength (3.82 ± 0.22, 3.34 ± 0.23, 3.74 ± 0.38, 3.47 ± 0.48 Mpa) and distinct micropores (4.44 ± 0.7, 1.54 ± 0.3, 2.59 ± 0.6, 3.59 ± 1.0 μm) on the surfaces of the scaffolds. Moreover, it was found that differences in the microstructure significantly facilitate the adhesion of bone marrow mesenchymal stem cells to scaffolds. Hence, the methods we provided hold a great promise for application in 3D printing techniques.
研究领域:
生物医学工程
·
组织工程
·
材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）