Biomedical Materials
·
2021
·Vol. 16
·期 5
·055001
·
IF 3.715
Junting Tao,Shixuan Feng,Bing Liu,Jiaqi Pan,Chaorong Li,Yingying Zheng
Accurate distinguish of cancer cells through fluorescence plays an important role in cancer diagnosis. Here we synthesized a blue fluorescent nitrogen-doped graphene quantum dots (N-GQDs) from citric acid and diethylamine via one-step hydrothermal synthesis method which was simple and quick to avoid by-products, and highlighted the binding sites to achieve precise combination. Due to the nitrogen element doping, amide II bond was amply obtained and abundant binding sites were provided for hyaluronic acid (HA) conjugation. N-GQDs solution with different pH value was then conjugated to HA via an amide bond for the recognition of human breast cancer cells (MCF-7 cells), and the formation of amide bond was more favorable under alkaline conditions. HA conjugated N-GQDs (HA-N-GQDs) were combined with CD44 which was over expressed on the surface of MCF-7 cells, resulting in MCF-7 cells performing stronger fluorescence. HA-N-GQDs showed high fluorescence, low toxicity, and good cytocompatibility, which held it play a role in fluorescence imaging for accurate identification of cancer cells.
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高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2021
·Vol. 44
·期 9
·2006-2014
·
IF 3.645
Yang Huang,Tiejie Wang,Zhengjin Jiang
In this study, a rapid and highly efficient method was developed for the separation of eight isoquinoline alkaloids using supercritical fluid chromatography. The separation conditions were carefully optimized including stationary phases, additives, backpressure, and temperature. Compared to high-performance liquid chromatography, the use of supercritical fluid chromatography could provide a 13 times faster separation. Subsequently, the method was validated and applied for the determination of eight alkaloids from different parts of Mahonia bealei (Fort.) Carr. (stem, root, leaf, and seed). The results indicated a good repeatability with relative standard deviations for overall precisions lower than 3.2%. The limit of detection was between 0.4 and 2.3 μg/mL while limit of quantitation ranged from 1.5 to 7.5 μg/mL. Recovery ranged from 95.7 to 102.5% indicating a validity of recovery. The content of total eight alkaloids was the highest in stem (66.0 μg/g) and root (65.1 μg/g) compared to leaf or seed. Moreover, anti-acetylcholinesterase activity for those extracts was evaluated by Ellman's colorimetric assay. As a result, the acetylcholinesterase inhibitory activity of the extracted samples was in the following decreasing order: stem > root > leaf or seed. In conclusion, the results indicated that supercritical fluid chromatography could be a useful tool for quality control of Mahonia bealei (Fort.) Carr. containing alkaloids as active compounds.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 48
·21228-21237
·
IF 3.288
Bo Hao,Wenbo Zhao,Dong Feng,Shengchao Xu,Yuan Chen
The enforcement of environmental law worldwide has attracted considerable attention in recent years towards capturing sulfur dioxide (SO2). The phase change absorption has turned out to be an effective way due to its industrially scalable characteristics. Herein, an aminosilane absorbent, namely dibutyl-trimethylsilanylmethyl-amine (DTSMA), was facilely synthesized by a substitution reaction of chloromethyltrimethylsilane and di-n-butylamine. The phase-change absorption performance of SO2 was systematically studied by using dimethyl silicone oil (DSO) as the solvent. The viscosity of pure DTSMA was only 1.29 mPa s, which is much lower than those of absorbents such as ionic liquids and deep eutectic solvents reported elsewhere, and the absorption product is found to be a charge transfer complex DTSMA SO2. The absorption capacity of DTSMA can reach 1.90 and 0.57 mol SO2 per mol DTSMA under 1 and 0.02 atm at 30 °C, respectively. In particular, the DTSMA/DSO solution can be not only almost regenerated within just 60 minutes at 100 °C, but also exhibited selective absorption towards SO2. All the results indicated that the synthesized DTSMA absorbent has enormous potential for application in capturing SO2 with benign circular desorption performance.
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JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Xiaoming Zhou,Zhuoyuan Chen,Zhiyong Guo,Haiping Yang,Jingai Shao,Xiong Zhang,Shihong Zhang
A series of dual metal incorporated CuCex-SAPO-34(x = 0–0.04) samples were synthesized using one-pot hydrothermal method with diethylamine as organic structure-directing agent for selective catalytic reduction of NO x by NH 3 . The catalytic properties were elucidated in detail with physicochemical properties being analyzed using various instruments. All the catalysts exhibited typical SAPO-34 crystal structures with high specific surface areas. With the dual-metal incorporation, the surface acidity and amount of isolated Cu 2+ , which may be active sites for NH 3 -SCR, were significantly enhanced. However, excessive Ce restrained the formation of isolated Cu 2+ due to its occupation of cationic sites. Therefore, the 0.05CuCe0.02-SAPO-34 exhibited high NO conversion (≥80%) at 168°C-500°C. Furthermore, the NH 3 -SCR mechanism over different catalysts was investigated in-situ DRIFTS experiments. For the 0.05Cu-SAPO-34, the adsorbed NH 3 species react with gaseous NO and following the E-R mechanism throughout the reaction temperature range. Meanwhile, adsorbed NO 2 was detected and reacted with the adsorbed NH 3 species according to the L-H mechanism in low-temperature region. In contrast, the NH 3 -SCR reaction over the 0.05CuCe0.02-SAPO-34 primarily followed the E-R mechanism throughout the temperature range. The L-H mechanism was cut off due to the loss of the adsorption ability of nitrous species at high temperatures., resulting in NO conversion decreasing.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Xue-Qi Zhang,Rong-Fang Shen,Xiao-Jing Guo,Xi Yan,Yan Chen,Jiang-Tao Hu,Wan-zhong Lang
Herein, a convenient and environment-friendly grafting and reduction strategy was used to anchor bimetallic Ag-Cu nanoparticles on polypropylene (PP) fabrics. The as-prepared samples were characterized by various techniques including FE-SEM, XRD, EDX, XPS and FT-IR. The as-prepared sample exhibits excellent activity on the catalytic reduction of highly concentrated 4-NP, and the synergistic effect between Ag and Cu nanoparticles was proved. The entire catalytic reduction was generally considered to obey pseudo-order kinetics, affording high conversion even after 15 successive recycles. These results showed that the PP based composite with Ag-Cu nanoparticles effectively provided superb catalytic efficiency and stability in 4-nitrophenol reduction, and the PP based composites were suitable in practical applications for the treatment of diverse waste water.
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JOURNAL OF MASS SPECTROMETRY
·
2020
·Vol. 55
·期 9
·e4528
·
IF 1.671
Feng-Jie Liu,Yan Jiang,Ping Li,Yang-Dan Liu,Gui-Zhong Xin,Zhong-Ping Yao,Hui-Jun Li
Fully understanding the chemicals in an herbal medicine remains a challenging task. Molecular networking (MN) allows to organize tandem mass spectrometry (MS/MS) data in complex samples by mass spectral similarity, which yet suffers from low coverage and accuracy of compound annotation due to the size limitation of available databases and differentiation obstacle of similar chemical scaffolds. In this work, an enhanced MN-based strategy named diagnostic fragmentation-assisted molecular networking coupled with in silico dereplication (DFMN-ISD) was introduced to overcome these obstacles: the rule-based fragmentation patterns provide insights into similar chemical scaffolds, the generated in silico candidates based on metabolic reactions expand the available natural product databases, and the in silico annotation method facilitates the further dereplication of candidates by computing their fragmentation trees. As a case, this approach was applied to globally profile the steroidal alkaloids in Fritillariae bulbus, a commonly used antitussive and expectorant herbal medicine. Consequently, a total of 325 steroidal alkaloids were discovered, including 106 cis -D/E-cevanines, 142 trans -D/E-cevanines, 29 jervines, 23 veratramines, and 25 verazines. And 10 of them were confirmed by available reference standards. Approximately 70% of the putative steroidal alkaloids have never been reported in previous publications, demonstrating the benefit of DFMN-ISD approach for the comprehensive characterization of chemicals in a complex plant organism.
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ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Lei Yu,Qiao Zhang,Qi Kang,Bin Zhang,Dazhong Shen,Guizheng Zou
The design and development of novel electrochemiluminescence (ECL) systems with near-infrared (NIR) emission beyond 800 nm are promising for ECL evolution, especially for improving the throughput of the spectrum-resolved multiplexing ECL assay and biological imaging. Herein a biocompatible and environmentally friendly luminophore, that is, the methionine-stabilized Au nanoclusters (Met-Au NCs), are proposed to achieve efficient aqueous ECL of ∼835 nm with triethanolamine as coreactant. The Met-Au NCs not only demonstrate a 75 times enhanced ECL compared with the traditional Au NCs with bovine serum albumin as a capping agent but also can be employed as ECL tags to label proteins with a methionine linker and enable a highly sensitive NIR ECL bioassay. A sandwich-type NIR ECL immunosensor is constructed with the Met-Au NCs as tags and α-fetoprotein (AFP) as a model analyte and exhibits a wide linearity range from 3 fg·mL–1 to 0.1 ng·mL–1 with a limit of detection of 1 fg mL–1 (S/N = 3) as well as desired selectivity.
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高影响因子
IONICS
·
2019
·Vol. 26
·期 2
·639-648
·
IF 2.289
Tang Jie,Dai Xinyi,Wu Fuzhong,Mai Yi,Wang Xiao,Jin Huixin,Gu Yijing,Xie Yanfang
Materials with nanotubular structure have attracted wide attention in electrochemical energy storage due to their good structural stability, high specific surface area, and internal and external wall activity. Here, we report a simple and efficient one-pot method for the synthesis of SiO 2 nanotubes with controlled aspect ratios. The influence of parameters, such as the monomer concentration and reaction time on the aspect ratio, were inspected systematically. The results confirmed that the aspect ratio of SiO 2 nanotubes increases with the reaction time before tetraethyl orthosilicate is added, and the length of SiO 2 nanotubes can also be altered by tuning the hydrazine hydrate/NiCl 2 ratio. The SiO 2 nanotubes as an anode material for lithium-ion batteries exhibited an initial discharge capacity of 625.5 mAh g −1 and maintained 232.5 mAh g −1 after 100 cycles at 40 mA/g, which is 75.7% relative to the second cycle, showing good electrochemical performance.
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TRIBOLOGY LETTERS
·
2019
·Vol. 67
·期 4
·1-11
·
IF 2.235
Ma Yanjun,Ye Yinping,Wan Hongqi,Chen Lei,Zhou Huidi,Chen Jianmin
A novel in-situ-synthesis strategy via one-pot thermal decomposition of a single-source precursor was developed to fabricate silver sulfide (Ag 2 S) nanoparticles with monodispersity and narrow size distribution in the polyamide-imide (PAI) nanocomposite coatings. The crystalline phases, size, morphologies, and growth mechanism of the in-situ-synthesized Ag 2 S nanoparticles were characterized by X-ray diffraction, ultrahigh-resolution field emission scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis. The enhancement effect of such in-situ-synthesized Ag 2 S nanoparticles on the microhardness and tribological behaviors of the PAI nanocomposite coatings were also investigated and compared with mechanically mixed Ag 2 S particles. Results show that the growth of Ag 2 S particles is greatly limited during the in-situ preparation process. The Ag 2 S nanoparticles with small size ranging from 100 to 130 nm are purely formed and exhibit monodispersibility in the PAI nanocomposite coatings. Besides, the appropriate Ag 2 S nanoparticles greatly promote the microhardness and antiwear performance of the PAI nanocomposite coatings, especially when their incorporation content reaches 5.0 wt%. The enhancement effects of these in-situ-synthesized Ag 2 S nanoparticles on microhardness and wear resistance of PAI nanocomposite coatings are superior to that of the mechanically mixed Ag 2 S particles. This is attributed to the improvement of cohesion strength and the load-carrying capacity of the PAI nanocomposite coatings enhanced by in-situ-synthesized Ag 2 S nanoparticles.
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JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Qifang He,Qian Lei,Shaoquan Huang,Yufang Zhou,Yuqin Liu,Sujin Zhou,Dong Peng,Xiulong Deng,Jun Xue,Xun Li,Hongdeng Qiu
The efficient and green extraction of bioactive ingredients from natural plants play a vital role in their corresponding drug effects and subsequent studies. Recently, deep eutectic solvents (DESs) have been considered promising new green solvents for efficiently and selectively extracting substances from varied plants. In this work, an environment-friendly DESs-based ultrasonic-assisted extraction (DESs-UAE) procedure was developed for highly efficient and non-polluting extraction of alkaloids from the roots of Stephania tetrandra (ST). A total of fifteen different combinations of DESs, compared with traditional organic solvents (methanol and 95% ethanol) and water, were evaluated for extraction of bioactive alkaloids (FAN and TET) from ST, and the results revealed that DESs system made up of choline chloride and ethylene glycol with mole ratio of 1:2 exhibited the optimal extraction efficiency for alkaloids. Additionally, a four-factor and three-level Box-Behnken design (BBD), a particular pattern of response surface methodology (RSM), was used to optimize extraction conditions. RSM results indicated that the maximum extraction yields of FAN, TET, and TA were attained 7.23, 13.36, 20.59 mg/g, respectively, within extraction temperature of 52 °C, extraction time of 82 min, DES water content of 23% (v/v), and liquid-solid ratio of 23 mL/g. The measured results were consistent with the predicted values. Notably, the optimized DES extraction efficiency of TA, according to the experimental data analysis, is 2.2, 3.3 and 4.1 times higher than methanol, 95% ethanol and water, respectively. Meanwhile, based on 3D response surface plots, interactive effects plots and contour maps, the effects of the aforementioned four essential factors on the extraction yield and their interactions on the response were visualized. The results revealed that the mutual interactions between extraction temperature and liquid-solid ratio exhibited positive effects on all responses, while extraction time and water content in DES posed a negative effect. Therefore, these results suggest that DESs, as a class of novel green solvents, with the potential to substitute organic solvent and water, can be widely and effectively applied to extract bioactive compounds from natural plants.
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高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2026
·
IF 3.9
Xiang Yu-Wei,Xing Ben-Kuo,Wang Xiang,Zhang Xin,Luo Yong,Zou Hai-Kui,Chu Guang-Wen,Chen Jian-Feng
The
Michael addition reaction of acrylonitrile (ACN) and dimethylamine
(DMA) is the main method for the industrial synthesis of 3-(dimethylamino)
propionitrile (DMAPN). However, inadequate mixing in the reaction
system can easily lead to ACN self-polymerization and low reaction
efficiency. In this work, a rotating packed bed (RPB) reactor with
excellent mixing performance was first applied to the Michael addition
reaction of ACN and DMA. First, the kinetics of the reaction was established,
revealing the characteristics of the reaction. Subsequently, the effects
of operating conditions on the reaction performance were investigated
in the RPB reactor. Under optimized conditions, the complete conversion
of ACN was achieved within 15 min in the RPB reactor, compared to
50 min required in the stirred-tank reactor (STR). Moreover, the RPB
reactor demonstrated broad applicability to various Michael addition
reaction substrates and delivered high yields. This study provides
theoretical guidance for the industrial application of an RPB reactor
in the Michael addition reaction.
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JOURNAL OF CHROMATOGRAPHY A
·
2026
·
IF 4
Mingjin Zhong,Qiuhan Zheng,Junhao Jiang,Yingqiu Zhang,Yilin He,Jinhang Li,Wenjin An,Yu Liu,Qiyang Zhao,Aihua Chen,Jing Li
First chiral separation of cyclaniliprole under basic reversed-phase conditions. • Reversed-phase liquid chromatography conditions were specifically optimized. • ECD combined with TDDFT calculations assign the absolute configuration. • MWCNTs significantly enhanced cleanup efficiency and target recoveries. • Satisfactory validation criteria to fortified and real samples were demonstrated. Herein, a rapid and highly sensitive high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was established and validated for the enantioselective determination of cyclaniliprole (CYCP) enantiomers in diverse plant-derived matrices including fruits (citrus, apple), vegetables (cabbage, tomato) and cereals (corn, soybean) matrices. The key innovation of the present work involves the first successful implementation of baseline enantiomeric separation of CYCP under basic reversed-phase conditions. By systematically screening five different chiral stationary phases and critically evaluating both acidic and basic mobile phases, basic conditions utilizing 0.05% diethylamine aqueous solution and acetonitrile were found to be pivotal. This novel basic reversed-phase system on a Superchiral® R-IC column not only afforded baseline separation within 6.5 min but also significantly enhanced both chiral resolution and elution efficiency, markedly reducing retention times compared to acidic conditions. A simple and effective reversed-phase dispersive solid-phase extraction (r-DSPE) procedure, employing multi-walled carbon nanotubes (MWCNTs) as purification sorbents, was optimized for sample preparation, with systematic evaluation of MWCNT type and quantity on purification efficiency and recovery. Comprehensive method validation was performed for linearity, accuracy, precision, sensitivity (LOQ), matrix effects, and stability. Under optimum conditions, mean recoveries for both enantiomers across all matrices ranged from 74.1% to 103.5% with RSDs < 15.2%. Excellent linearity (r ≥ 0.9991) was demonstrated over 0.5–500 μ g/L using weighted (1/x) least squares regression. The LOQ was established at 0.005 mg/kg for both enantiomers in all six matrices. Validation data, coupled with successful application to the analysis of real samples confirming method applicability, demonstrate that this robust protocol is suitable for the enantioselective determination of CYCP residues in various agricultural produce, offering a streamlined solution for monitoring chiral pesticides. Download: Download high-res image (269KB) Download: Download full-size image
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