TALANTA
·
2016
·
IF 4.035
Fengli Qu,Haimeng Pei,Rongmei Kong,Shuyun Zhu,Lian Xia
Using sterculia lychnophora seeds as precursors for the first time, fluorescent carbon dots (CDs) were synthesized by simple hydrothermal treatment . The quantum yield of as-synthesized CDs was 6.9% by using quinine sulfate as the reference. The fluorescence of CDs could be effectively quenched by a MnO 2 nanosheet based on fluorescence resonance energy transfer (FRET). Ascorbic acid (AA) could reduce MnO 2 to Mn 2+ and result in the destruction of the MnO 2 nanosheets, which could induce the fluorescence recovery of the CDs. In particular, alkaline phosphatase (ALP) could bio-catalyze acid 2-phosphate (AAP) hydrolysis to AA. Here, an efficient fluorescence probe based on a CDs-MnO 2 nanosheet for rapid and selective detection of ALP was reported for the first time. Excellent performance for the detection of ALP was observed with high sensitivity and a detection limit of 0.4 U/L owing to the low background. The detection of ALP in human serum was conducted with satisfactory results, demonstrating its potential applications in clinical diagnosis.
研究领域:
生物化学
·
医学诊断
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DOI
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2018
·Vol. 18
·期 3
·1709-1715
·
IF 1.354
Cai Qiyong,Zhang Lin,Geng Xin,Ge Jia,Li Zhaohui
Glutathione (GSH), a thiol-containing tripeptide, has a pivotal role in maintaining the normally physiological environment in biology. Here, we report a novel self-assembly fluorescence resonance energy transfer (FRET) sensing platform for GSH detection by using the environment-friendly
Cu:Zn–In–S/ZnS quantum dots (QDs) and MnO2 nanosheets. In this assay, fluorescence of Cu:Zn– In–S/ZnS QDs could be quenched by MnO2 nanosheets based on FRET. Upon the addition of GSH, MnO2 nanosheets were reduced into Mn2+
ions because of the rapid redox reaction, and consequently the fluorescence of QDs was recovered. A good linear relationship was obtained from 1 to 20 μM for the detection of GSH with a low detection limit of 500 nM. Meanwhile, the sensing system also exhibited high selectivity against
other electrolytes and biomolecules. Moreover, the satisfactory results in the determination of GSH in serum samples indicate that the assay holds great potential for detecting GSH in biological fluids. This method shows many advantages including high sensitivity, short assay time, good simplicity
as well as label-free and environmental friendly, which is expected to facilitate the GSH quantification relating to biological and biomedical studies.
研究领域:
生物化学
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分析化学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2024
·
IF 4.1
Yuhan Yang,Yicheng Yang,Xiaocheng Qi,Tonghao Zeng,Yuan Wang,Shaojun Yuan
Metal oxide have attracted much attention as a pseudocapacitive electrode for high energy density supercapacitors (SCs). However, the battery-like charge storage mechanism based on ions diffusion and valence change for metal oxide would lead to a poor cycling stability and unsatisfied rate performance. Herein, cobalt ions pre-intercalation MnO 2 nanosheet arrays on Ni foam (Co-MnO 2 /NF) was prepared as the cathode for SCs. The capacitive behavior of MnO 2 was enhanced via cobalt introduction. The optimized Co-MnO 2 /NF-2 demonstrated improved pseudocapacitance and stability, with a specific capacitance of 405.6 mF cm −2 at 1 mA cm −2 . It retained 90.5 % of its initial capacitance at 10 mA cm −2 after 5000 cycles, which was superior to that of pristine MnO 2 (222.4 mF cm −2 , 65.0 % after 1000 cycles), Co-MnO 2 /NF-1 (295.2 mF cm −2 , 85.9 % after 1000 cycles), and Co-MnO 2 /NF-3 (349.9 mF cm −2 , 84.9 % after 1000 cycles). The asymmetric aqueous supercapacitor consisted of Co-MnO 2 /NF-2 and N-doped porous carbon fiber (NPCF) electrodes with an operating potential of 2.0 V reaches a high areal energy density of 0.06 mWh cm −2 at a power density of 0.5 mWh cm −2 .
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Lv Jie,He Chengcai,Cao Jingcheng,Shuai Qiuyan,Liu Xifang,Li Meng,Lin Yulong
The abnormal expression of acetylcholinesterase (AChE) is linked to the development of various diseases. Accurate determination of AChE activity as well as screening AChE inhibitors (AChEIs) holds paramount importance for early diagnosis and treatment of AChE-related diseases. Herein, a fluorescent and colorimetric dual-channel probe based on gold nanoclusters (AuNCs) and manganese dioxide nanosheets (MnO 2 NSs) was developed. The fluorescence of AuNCs was suppressed in the presence of MnO 2 NSs, providing a platform for fluorescence-based detection. For colorimetric, the nanocomposites exhibited oxidase-like activity, rapidly catalyzing 3,3′,5,5′-tetramethylbenzidine (TMB) to generate a blue color. Thiocholine (TCh), produced through the enzymatic reaction of acetylthiocholine (ATCh) in the presence of AChE, can reduce the MnO 2 NSs, thus recovering the suppressed fluorescence of AuNCs and decreasing oxidase-like activity. Based on this principle, a dual-mode assay for AChE detection was achieved. Compared to the commonly used single-signal detection, multi-mode detection can offer reliable and accurate results, due to its inherent self-validation and self-regulation capabilities. Under optimum conditions, the limit of detection (LOD) for AChE activity was 0.067 mU mL –1 (fluorescent mode) and 0.042 mU mL –1 (colorimetric mode). Moreover, the probe realized AChE detection in biological samples and AChEIs screening. This work showed the great prospects for early diagnosis of AChE-related diseases. Graphical
研究领域:
生物化学
·
纳米医学
·
疾病诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 5
·1-9
·
IF 5.833
An Xuanxuan,Chen Ruchun,Chen Qizhen,Tan Qin,Pan Shuang,Liu Hui,Hu Xiaoli
A MnO 2 nanosheet-assisted ratiometric fluorescence probe based on carbon quantum dots (CQDs) and o-phenylenediamine (OPD) has been developed for the detection of the anticancer drug 6-mercaptopurine (6-MP). CQDs with strong fluorescence are synthesized via the one-step hydrothermal method. MnO 2 nanosheets as an oxidase-mimicking nanomaterial directly oxidize OPD into 2,3-diaminophenazine (DAP) which has a fluorescence emission at 570 nm, whereas the fluorescence of CQDs at 445 nm is then reduced by the DAP through the inner filter effect (IFE) under a single excitation wavelength (370 nm). After adding 6-MP, MnO 2 nanosheets can be reduced to Mn 2+ and lose their oxidase-like property, blocking the IFE with the fluorescence decrease of DAP and fluorescence increase of CQDs. The novel ratiometric fluorescence probe exhibits considerable sensitivity toward 6-MP and linear response is in the 0.46–100.0 μmol L −1 concentration range with the detection limit of 0.14 μmol L −1 . Furthermore, the probe shows good selectivity when exposed to a series of interfering other organic and inorganic compounds, and biomolecules and can be applied to the detection for 6-MP in human serum samples and pharmaceutical tablets. Satisfactory recoveries of 6-MP in human serum samples are in the range 96.1–110.9% with the RSD of 1.4 to 3.2%. The amount of 6-MP is successfully estimated as 49.3 mg in pharmaceutical tablet with the RSD of about 2.2%. Graphical abstract
研究领域:
分析化学
·
药物分析
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 19
·4887-4894
·
IF 4.157
Zhang Luwei,Xin Fuli,Cai Zhixiong,Zhao Hong,Zhang Xiaolong,Yao Cuiping
Azodicarbonamide (ADA), as a dough conditioner food additive in flour, can be turned into toxic biurea and semicarbazide after high temperature processing. Hence, the using of ADA in food material should be strictly controlled, and the detection of ADA is very important for consumers’ safety and health. Herein, a simple and fast colorimetric strategy has been developed for ADA detection based on the MnO 2 nanosheets-3,3′,5,5′-tetramethylbenzidine (TMB)-glutathione (GSH) as oxidative sensing system (MnO 2 -TMB-GSH). Since the ADA can selectively react with GSH via oxidizing the sulfydryl (-SH) group of GSH to disulfide bond (S-S), which makes GSH unable to reduce MnO 2 nanosheets and restore its oxidase-like activity. The absorbance changes of the TMB solution depended on ADA content. The MnO 2 -TMB-GSH colorimetric platform can detect the ADA with a linear range of 10 μmol L −1 (11.6 ppm) to 400 μmol L −1 (464 ppm), and the limit of detection (LOD) is 3.3 μmol L −1 (3.51 ppm). Some potential interferences in real sample were tested and did not affect the MnO 2 -TMB-GSH colorimetric platform for ADA detection. Furthermore, the sensing platform was applied for detecting ADA in real flour sample with a recovery of 96%–105% (RSD < 5%). This colorimetric method can effectively and rapidly detect ADA additives in flour less than the prescribed standard (45 mg kg −1 ), which shows a great potential for visualization analysis and on-site detection of ADA in flour. Graphical abstract
A simple and fast colorimetric strategy has been developed for azodicarbonamide (ADA) detection based on the MnO 2 nanosheets-3,3′,5,5′-tetramethylbenzidine (TMB)-glutathione (GSH) as oxidative sensing system (MnO 2 -TMB-GSH).
研究领域:
食品安全
·
分析化学
·
生物传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 5.31
Shaopeng Rong,Pengyi Zhang,Jinlong Wang,Fang Liu,Yajie Yang,Guoliang Yang,Song Liu
Ultrathin manganese dioxide (MnO 2 ) nanosheets with the thickness of 1–2 nm were synthesized by chemical oxidation of Mn 2+ ions in the presence of tetramethylammonium cations in an aqueous solution. The composite material MnO 2 /PET (polyethylene terephthalate) was prepared by impregnating PET into MnO 2 nanosheets colloid. The impregnation time has a great influence on HCHO adsorption and removal efficiency. When the PET was impregnated in nanosheets colloid for 1 h at room temperature, the concentration of HCHO decreased quickly within initial 10 min and finally reached about 81% removal efficiency with an initial HCHO concentration of ∼200 ppm. Moreover, the used MnO 2 /PET can be simply regenerated by heating at 105 °C without any rinse treatment. Based on in-situ DRIFTS and TG-MS studies, the mechanism of formaldehyde removal and regeneration was proposed. All the results showed that as-synthesized MnO 2 /PET can be used as an effective, affordable, and recyclable materials to removal and oxide toxic HCHO in indoor air.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 9
·1429
·
IF 5.719
Shuo Liu,Peng Zhang,Yuming Miao,Chenmin Li,Yu-e Shi,Jinhua Liu,Yun-kai Lv,Zhenguang Wang
Selective and sensitive identification of paraoxon residue in agricultural products is greatly significant for food safety but remains a challenging task. Herein, a detection platform was developed by integrating Cu nanoclusters (Cu NCs) with MnO2nanosheets, where the fluorescence of Cu NCs was effectively quenched. Upon introducing butyrylcholinesterase and butyrylcholine into the system, their hydrolysate, thiocholine, leads to the decomposition of the platform through a reaction between the MnO2nanosheets and thiol groups on thiocholine. The electron-rich groups on thiocholine can further promote the fluorescence intensity of Cu NCs through host–guest interactions. Adding paraoxon results in the failure of fluorescence recovery and further promotion, which could be utilized for the quantitative detection of paraoxon, and a limit of detection as low as 0.22 ng/mL can be achieved. The detection platform shows strong tolerance to common interference species, which endows its applications for the detection of paraoxon in vegetables and fruit. These presented results not only open a new door for the functionalization of metal nanoclusters but also offer an inspiring strategy for analytic techniques in nanomedicine and environmental science.Keywords:organophosphorus pesticides;fluorometry assay;metal nanoclusters;MnO2nanosheets;fluorescence quenching
研究领域:
食品安全
·
分析化学
·
纳米医学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemistry-An Asian Journal
·
2018
·Vol. 13
·期 5
·545-551
·
IF 3.692
Xu Ou,Qi Li,Dan Xu,Jiangna Guo,Feng Yan
Graphical N-CNTs@MnO 2 nanocomposites have been successfully prepared by in situ growing MnO 2 nanosheets on N-doped carbon nanotubes (CNTs) derived from polypyrrole nanotubes. The as-prepared nanocomposites showed good capacitance and cycling stability as supercapacitor electrode materials. Nitrogen-doped porous carbon nanotubes@MnO 2 ( N -CNTs@MnO 2 ) nanocomposites are prepared through the in situ growth of MnO 2 nanosheets on N-CNTs derived from polypyrrole nanotubes (PNTs). Benefiting from the synergistic effects between N-CNTs (high conductivity and N doping level) and MnO 2 nanosheets (high theoretical capacity), the as-prepared N-CNTs@MnO 2 -800 nanocomposites show a specific capacitance of 219 F g −1 at a current density of 1.0 A g −1 , which is higher than that of pure MnO 2 nanosheets (128 F g −1 ) and PNTs (42 F g −1 ) in 0.5 m Na 2 SO 4 solution. Meanwhile, the capacitance retention of 86.8 % (after 1000 cycles at 10 A g −1 ) indicates an excellent electrochemical performance of N-CNTs@MnO 2 prepared in this work.
研究领域:
材料科学
·
能源存储
✓ 已验证
DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2024
·
IF 2.6
Zhao Fengju,Guo Hui,Yang Wei,Guo Lili,Li Jiaxin,Chen Hanqi
Acetylcholinesterase (AChE) plays an important role in the treatment of human diseases, environmental security and global food supply. In this study, the simple fluorescent indicators and MnO 2 nanosheets were developed and integrated to establish a ratiometric fluorescence sensing system for the detection of AChE activity. Two fluorescence signals could be recorded independently at the same excitation wavelength, which extended the detection range and enhanced the visibility of results. Fluorescence of F-PDA was quenched by MnO 2 nanosheets on account of inner filtering effect. Meanwhile, the nonfluorescent OPD was catalytically oxidized to 2,3-diaminophenazine by MnO 2 nanosheets. The acetylcholine (ATCh) was catalytically hydrolyzed by AChE to enzymatic thiocholine, which decomposed MnO 2 to Mn 2+ , recovered the fluorescence of F-PDA and reduced the emission of ox-OPD. Utilizing the fluorescence intensity ratio F 468 /F 558 as the signal readout, the ratiometric fluorescence method was established to detect AChE activity. Under the excitation wavelength of 410 nm, the ratio F 460 /F 558 against the AChE concentration demonstrated two linear relationships in the range 0.05 -1.0 and 1.0–50 U·L − 1 with a limit of detection (LOD) of 0.073 U·L − 1 . The method was applied to the detection of AChE activity and the analysis of the inhibitor Huperzine-A. Due to the advantages of high sensitivity and favorable selectivity, the method possesses an application prospect in the activity deteceion of AChE and the screening of inhibitors. Graphical Abstract
研究领域:
生物化学
·
分析化学
·
纳米技术
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Jin Li,Yun-Yun Wei,Xiao-Peng Liu,Zhang-Run Xu
As a crucial enzyme involved in melanogenesis, tyrosinase plays an important role in the diagnosis of melanoma. Herein, a novel and simple photothermal quantitative method based on manganese dioxide nanosheets-mediated etching of gold nanorods was established for tyrosinase activity assay with a simple household thermometer as the readout. Gold nanorods were etched by manganese dioxide nanosheets in acid condition at room temperature with a fast and stable etching efficiency. With the etching of gold nanorods, the photothermal conversion efficiency decreased gradually with the change of the aspect ratio of gold nanorods, leading to the decrease of the temperature increment of the solution by degrees. The gold nanorods have an unparalleled photothermal conversion efficiency, and the increment of temperature caused by the photothermal effect of gold nanorods has a span as wide as nearly 17 °C within one minute of laser irradiation . A household thermometer, instead of a spectrometer or any other professional and costly instruments, was used as a signal reader to achieve the visual quantitative detection, which makes this method possible for point-of-care testing. The photothermal platform was used successfully for the determination of tyrosinase and its substrate dopamine with wider linear ranges from 1.0 to 70 U/mL and 2.0–150 μM and detection limits as low as 0.34 U/mL and 0.92 μM, respectively. Moreover, the inhibition efficiency of kojic acid on tyrosinase activity was investigated, proving the photothermal quantitative method will be a potential platform for screening inhibitors of tyrosinase.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
癌症诊断
✓ 已验证
产品关联
DOI
高影响因子
SENSORS
·
2019
·Vol. 19
·期 2
·228
·
IF 3.031
Shurong Tang,Xiuhua You,Quanhui Fang,Xin Li,Guangwen Li,Jinghua Chen,Wei Chen
A novel turn-on fluorescence assay was developed for the rapid detection of glutathione (GSH) based on the inner-filter effect (IFE) and redox reaction. Molybdenum disulfide quantum dots (MoS2QDs), which have stable fluorescent properties, were synthesized with hydrothermal method. Manganese dioxide nanosheets (MnO2NSs) were prepared by exfoliating the bulk δ-MnO2material in bovine serum albumin (BSA) aqueous solution. The morphology structures of the prepared nanoparticles were characterized by transmission electron microscope (TEM). Studies have shown that the fluorescence of MoS2QDs could be quenched in the presence of MnO2NSs as a result of the IFE, and is recovered after the addition of GSH to dissolve the MnO2NSs. The fluorescence intensity showed a good linear relationship with the GSH concentration in the range 20–2500 μM, the limit of detection was 1.0 μM. The detection method was applied to the analysis of GSH in human serum samples. This simple, rapid, and cost-effective method has great potential in analyzing GSH and in disease diagnosis.
研究领域:
分析化学
·
生物医学
✓ 已验证
DOI
高影响因子
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