SENSORS AND ACTUATORS B-CHEMICAL
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2026
·
IF 8.3
Siqi Wei,Renjie Li,Wenxi Wang,Dan Huang,Xiandeng Hou,Li Wu
A novel ML-assisted SERS platform was developed for meat freshness evaluation. • Core-shell GNU/p-MBA@MIL-101 substrate enabled sensitive and stable SERS sensing. • Embedded p-MBA served as an internal standard and selectively reacted with VAs. • NH 3 , MA, PUT and CAD were quantified with ppb-level detection limits. • CNN analysis achieved 98.5% accuracy for meat freshness classification. Accurate and rapid evaluation of meat freshness remains challenging due to the complexity of spoilage markers and environmental interferences. Herein, a novel machine learning (ML)-assisted SERS platform was developed based on a core-shell GNU/p-MBA@MIL-101 substrate. Gold nanourchins (GNU) modified with p-mercaptobenzoic acid (p-MBA) were coated with MIL-101(Fe), enabling efficient gas capture and Raman signal enhancement. The embedded p-MBA was used as an internal standard (IS) and selectively reacted with volatile amines (VAs), to enable SERS detection with high sensitivity, reproducibility and anti-interference performance. Typical spoilage markers (NH 3 , MA, PUT and CAD) were quantitatively detected with limits of detection down to the ppb level. Convolutional neural networks (CNNs) were trained on SERS spectra collected from meat samples stored under different conditions, achieving a classification accuracy of 98.5%. This ML-assisted SERS strategy provides a reliable and accurate assessment of meat freshness, demonstrating strong potential for intelligent monitoring in food safety and quality control. Download: Download high-res image (119KB) Download: Download full-size image
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Peng Nie,Xiang Gao,Xuefei Yang,Yang Zhang,Haijie Lu,Heng Wang,Zhi Zheng,Yizhong Shen
Food safety concerns about the authenticity of soy product freshness have increased due to high demand from public. Developing an accurate and convenient monitoring method for freshness authenticity is crucial for safeguarding food safety. From this motive, this study employed PtPd NPs to encapsulate tetraphenylethylene (TPE) for engineering an AIE-based fluorescent nanozyme (PtPd NPs@TPE) with oxidase-like activity, achieving the ratiometric fluorescence monitoring of putrescine (PUT) to judge the freshness authenticity of soy products. In this design, PUT acted as an antioxidant and inhibited the oxidation process of PtPd NPs@TPE to o -phenylenediamine (OPD), leading to the reduction of oxidative product 2,3-diaminophenothiazine (DAP) alone with the weaken of yellow fluorescence from DAP at 552 nm and bright of bule fluorescence from PtPd NPs@TPE at 442 nm. On this basis, a ratiometric fluorescence strategy integrated with smartphone-based sensor was developed for PUT with acceptable results to combat food freshness fraud of soy products.
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高影响因子
JOURNAL OF INSECT PHYSIOLOGY
·
2023
·
IF 2.2
Wan-Xue Li,Jing-Xiang Chen,Chuan-Chuan Zhang,Min-Shi Luo,Wen-Qing Zhang
The brown planthopper (BPH, Nilaparvata lugens ), a major insect pest of rice, can make a shift in wing dimorphism to adapt to complex external environments. Our previous study showed that NlODC ( Ornithine decarboxylase in N. lugens ) was involved in wing dimorphism of the brown planthopper. Here, further experiments were conducted to reveal possible molecular mechanism of NlODC in manipulating the wing dimorphism. We found that the long-winged rate (LWR) of BPH was significantly reduced after RNAi of NlODC or injection of DFMO (D, L-α-Difluoromethylornithine), and LWR of males and females significantly decreased by 21.7% and 34.6%, respectively. Meanwhile, we also examined the contents of three polyamines under DFMO treatment and found that the contents of putrescine and spermidine were significantly lower compared to the control. After 3rd instar nymphs were injected with putrescine and spermidine, LWR was increased significantly in both cases, and putrescine was a little bit more effective, with 5.6% increase in males and 11.4% in females. Three days after injection of ds NlODC , injection of putrescine and spermidine rescued LWR to the normal levels. In the regulation of wing differentiation in BPH, NlODC mutually antagonistic to NlAkt may act through other signaling pathways rather than the classical insulin signaling pathway. This study illuminated a physiological function of an ODC gene involved in wing differentiation in insects, which could be a potential target for pest control.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Junjun Wang,Yue Tang,Jia Zheng,Zhengmin Xie,Jianli Zhou,Yuangen Wu
Herein, a colorimetric biosensor for histamine detection in meat is first established based on the enhancement of DNAzyme with peroxidase-mimic activity. Histamine can boost the generation of G-quadruplex sequences, and make them more easily bond with hemin to produce many DNAzyme molecules. In addition, histamine increases the affinity of DNAzyme to the substrate 3,3′,5,5′-tetramethylbenzidine (TMB). Therefore, the obtained DNAzyme can catalyze H 2 O 2 and dissolved oxygen to produce many reactive oxygen species (ROS), which cause the TMB molecule to lose two electrons and generate yellow products, exhibiting a clear absorption peak at 450 nm. The colorimetric biosensor has excellent sensitivity, and the detection limit is as low as 38 μg·L −1 for histamine. Moreover, the biosensor has high selectivity and anti-interference ability, and exhibits a good recovery rate in actual meats. The above results show that the strategy has potential for application in the detection of trace histamine in meats.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Xiang Gao,Peng Nie,Peiran Li,Zhi Zheng,Jieshun Cheng,Ying Gu,Yizhong Shen
To combat food freshness fraud, it is urgent to develop a method which could realize the detection of biogenic amines (BAs) present in food. In our study, we developed a colorimetric and ratiometric fluorescence dual-mode sensor which integrated with silver metallization-based response system of AIE liposome + OPD + RSM + Ag + toward BAs in foods for fighting freshness fraud. With the hydrolysis from the alkaline of BAs to resorcinol monoacetate (RSM), the production resorcinol (RS) could metallize silver ion (Ag + ) to silver atoms (Ag 0 ) which could lead to a BAs concentration-dependent decrease of the oxidation product 2,3-diaminophenothiazine (DAP) of Ag + to o -phenylenediamine (OPD). As a result, the dual-mode sensor has a low detection limit and wide linear range in the spiked detection of soy products, pork and milk samples for BAs. Thus, providing a reliable method for food safety and forestalling food freshness fraud.
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PLoS One
·
2023
·Vol. 18
·期 8
·e0289563
·
IF 3.7
Dongdong Cao,Yutao Huang,Gaofu Mei,Sheng Zhang,Huaping Wu,Tiyuan Zhao
Chilling stress is an important constraint for kale seed germination and seedlings establishment. It is vital to develop an effective approach to enhance kale seed germination ability under chilling stress. The present study reported that spermidine (Spd) could improve seed chilling tolerance in two kale cultivars ‘Nagoya’ (MGW) and ‘Pigeon’ (BB) during germination. The results showed that MGW was cold tolerant with a 90.67% germination percentage (GP) under chilling stress, while BB was cold sensitive with a 70.67% GP under chilling stress. Spd content in MGW and BB seeds during seed germination were up-regulated and down-regulated by chilling stress, respectively. Besides, chilling stress apparently decreased the gibberellin (GA) and ethylene (ET) contents, while increased the levels of abscisic acid (ABA) and reactive oxygen species (ROS) in MGW and BB seeds during germination. Exogenous Spd application increased GA, ET contents and decreased ABA content through regulating the gene expressions of metabolic-related enzymes, thus effectively alleviating the low temperature damage on kale seed germination. Besides, Spd significantly increased the activities of superoxide dismutase (SOD) and peroxidase (POD), and reduced the levels of hydrogen peroxide (H 2 O 2 ) and superoxide anion (O 2 · - ). The present study demonstrated that endogenous Spd metabolism plays an important role in kale seed germination under chilling stress. The effect of exogenous Spd on the metabolism of endogenous Spd, GA, ABA, ET and antioxidant enzymes might be the important reason for promoting the kale seed vigor at low temperature.
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DYES AND PIGMENTS
·
2023
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IF 4.5
Zekun Shi,Yongning Wu,Xue Zhang,Yanyan Gao,Luqin Qiao,Qin Hou,Minglin Wang
Fluorescent cuprous iodide (CuI)-based coordination polymers (CuI-CPs) were widely used in the sensor field for high selectivity and sensitivity. Here, a new CuI-CP fluorescent probe was fabricated for the detection of spermine, spermidine and cysteine with dual-mode, which was capable of both convenient colorimetric analysis and ratiometric fluorescent detection. The fluorescence of CuI-CP solution was quenched and the solution changed from white to purple upon addition of spermine or spermidine, while the solution changed from white to yellow for cysteine. This probe exhibited selective, sensitive, rapid and linear response to spermine, spermidine and cysteine with a limit of detection of 0.57, 1.06 and 1.18 μM, respectively. Furthermore, cellulose acetate was used as the matrix to fabricate intelligent fluorescent film for real-time and visual monitoring the freshness of pork samples based on distinct fluorescence changes under UV light. This work realized a rapid, efficient, and profitable method for visually evaluating food quality.
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Cell Reports
·
2023
·
IF 8.8
Ting Zeng,Jie Yin,Ziwen Liu,Zhaoxing Li,Yu Zhang,Yang Lv,Mei-Ling Lu,Min Luo,Meirong Chen,Yibei Xiao
Summary The type V-K CRISPR-associated transposons (CASTs) allow RNA-guided DNA integration and have great potential as a programmable site-specific gene insertion tool. Although all core components have been independently characterized structurally, the mechanism of how the transposase TnsB associates with AAA+ ATPase TnsC and catalyzes donor DNA cleavage and integration remains ambiguous. In this study, we demonstrate that TniQ-dCas9 fusion can direct site-specific transposition by TnsB/TnsC in ShCAST. TnsB is a 3′-5′ exonuclease that specifically cleaves donor DNA at the end of the terminal repeats and integrates the left end prior to the right end. The nucleotide preference and the cleavage site of TnsB are markedly different from those of the well-documented MuA. We also find that TnsB/TnsC association is enhanced in a half-integration state. Overall, our results provide valuable insights into the mechanism and application expansion of CRISPR-mediated site-specific transposition by TnsB/TnsC.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Bobo Hu,Nana Zhang,Huihui Li,Chong Sun
Sensitive and reliable quantification of histamine (His) is essential to ensure food safety. In this study, a sensing material consisting of reduced graphene oxide/polypyrrole (rGO-PPy) composites is successfully prepared by a two-step synthesis method and verified by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, ultraviolet-visible (UV-vis) spectroscopy, X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). Based on rGO-PPy, an electrochemical method is established, and the electrochemical behaviours are studied by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The experimental parameters, such as the detection method, the concentration and volume of rGO-PPy modified on GCE, and the pH value of the buffer solution, are optimized. Under the optimized conditions, the linear equation of the electrochemical sensor to His is I (μA) = 0.01047c + 0.1842 (R 2 = 0.9970) with a linear range of 10~800 μM, and the detection limit is 3.01 μM. Practical samples are also detected, which proves that the sensor has great application prospects. Therefore, this idea and method will make an excellent contribution to food safety.
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NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 16
·7588-7594
·
IF 3.3
Jianfeng Yan,Quanbin Fu,Shikai Zhang,Xianbao Shi,Yuanhong Zhang,Juying Hou,Junling Duan,Shiyun Ai
As a kind of organic basic compound containing nitrogen, biogenic amine is released from food spoilage and considered as an indicator for monitoring food freshness. Herein, an excellent dual-emission ratiometric fluorescent sensor for eight kinds of biogenic amines was established via the facile mixing of pH-responsive carbon dots and Rhodamine B. The developed sensor shows a rapid response (10 s), low limit of detection (0.733–3.943 μM) and a wide linear range (0–150 μM). More importantly, a portable fluorescent filter paper was prepared by simple dispersing the filter paper in a solution mixture of carbon dots and Rhodamine B. The obtained fluorescent filter paper based sensor showed an obvious color change in the presence of biogenic amine vapor derived from shrimp and mushroom. The results revealed that this sensor could realize the on-site and visual detection of biogenic amines in real food samples, making it suitable for potential application in food safety monitoring.
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Food Analytical Methods
·
2023
·Vol. 16
·期 5
·909-917
·
IF 2.9
Xu Yanfen,Lu Ying,Yu Jianna,Liu Wen,Jing Guoxing,Li Wenshan,Liu Wenjie
Tuna is one of the most popular fish and therefore among the most commercially valuable seafood. However, inadequate refrigeration during the whole supply chain causes serious economic loss and food poisoning. One important result of tuna spoilage is the accumulation of biogenic amines such as histamine, which can be used as a sensitive indicator for Tuna freshness. Thus, the development of rapid, sensitive, and economic analytical methods for biogenic amines in Tuna is highly desired. This research developed a method that combines high-performance liquid chromatography (HPLC) and electrospray ionization ion mobility spectrometry (ESI-IMS) to detect 7 kinds of biogenic amines including tryptamine, tyramine, spermidine, cadaverine, β-phenylethylamine, putrescine, and histamine in tuna. The limit of detections (LODs) for 7 biogenic amines are 0.01, 0.02, 0.02, 0.17, 0.03, 0.04, and 0.15 mg L −1 respectively. The linear range of histamine and spermidine is 2–100 mg kg −1 , and the linear range of other biogenic amines is 0.5–10 mg L −1 . The recoveries of biogenic amines are between 69.39 and 112.65%. Compared to the traditional HPLC-based analytical method, the proposed method does not involve any derivatization steps, which greatly decreased the sample preparation time and cost. Another advantage of the developed method is the supplementary separation to the HPLC, in which coeluted biogenic amines can clearly be separated in the ion mobility dimension. The proposed method is applied to determining the concentration of biogenic amines in tuna storage, which showed that this method can quickly and accurately monitor the changes of biogenic amines in tuna during storage.
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Nanomedicine-Nanotechnology Biology and Medicine
·
2022
·
IF 6.458
Qiming Yin,Xiang Song,Peng Yang,Wen Yang,Xinyu Li,Xuejun Wang,Shengqi Wang
Liver injury caused by hepatitis is the pathological basis of varied hepatic diseases with high morbidity and mortality. Although siRNA appears promising in therapeutics of hepatitis, efficient and safe delivery remains a challenge. In this study, we developed a new strategy of incorporating glycyrrhizic acid (GA) and polyene phosphatidylcholine (PPC) into lipid nanoparticles (GA/PPC-modified LNPs), which was capable of promoting cellular uptake, enhancing gene-silencing, reducing cytotoxicity and improving siRNA stability. GA/PPC-modified LNP and siRNA lipoplex targeting NF-κB, a key mediator of inflammation, mitigates acute liver injury, as assessed by liver histology , hematological and pro-inflammatory cytokine analysis. Furthermore, GA/PPC-modified LNPs reveal efficiently intracellular delivery of antisense oligonucleotides (ASOs) and mRNA inhibiting viral infection. In conclusion, GA/PPC-modified LNPs could be used as a promising delivery system for nucleic acid-based therapy.
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