COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Jiyao Xu,Lili Sun,Xiangjun Guo,Hongmei Zhang,Xihui Zhao
Green, blue and pink fluorescent carbon dots (o/m/p-CDs) were prepared by one-step hydrothermal method with three isomers (o/m/p) of phenylenediamine as nitrogen and carbon sources and ethanol as solvent. The obtained carbon dots exhibited different luminescence behaviors, and the luminescence behaviors were affected by solvent and pH. More interestingly, m-CDs exhibited obvious discoloration behavior under different pH conditions and had excellent linear relationship under acidic and basic conditions, respectively. Furthermore, we combined m-CDs with test paper, PVA film, and cotton fabric, respectively, to prepare composites for visual pH detection. The as-prepared composite material has excellent visual detection effect for a small amount of test sample (<5 μL) in the fine pH range (0.5 unit), and could be applied to trace pH detection.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 9
·6824-6830
·
IF 3.201
Guojuan Ren,Yuxi Meng,Qi Zhang,Mingyu Tang,Baoya Zhu,Fang Chai,Chungang Wang,Zhongmin Su
Nitrogen-doped carbon dots (NCDs) were synthesised using a simple and straightforward solvothermal method. The resulting spherical NCDs showed stable fluorescence and a prominent fluorescence quantum yield (67%) with an average size of 3.52 nm. The fluorescence of the NCDs was quenched effectively by mercury ions (Hg2+), exhibiting a detection limit of 0.65 μM. Upon addition of a saturated solution of ethylenediaminetetraacetic acid to a dispersion of the NCDs/Hg2+ complex, the fluorescence of the NCDs underwent significant immediate recovery. Due to their favorable biocompatibility, NCDs were also demonstrated to be efficient for HeLa cell imaging. The detection of Hg2+ by NCDs could be realized in environmental water samples and living cells, with excellent results. Moreover, the as-synthesized fluorescent NCD nanopowders were used for visualizating latent fingerprints (LFPs) with significant third level detail.
研究领域:
生物化学
·
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2021
·Vol. 56
·期 9
·5543-5554
·
IF 4.22
Huang Mengjun,Peng Di
In forensic science, the demand for the discovery and extraction of latent fingermarks at crime scenes in a fast and convenient way is still urgent. Herein, a rutile Cr- and Sb-codoped TiO 2 nanoparticle was synthesized via homogeneous precipitation and was characterized by scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, Raman spectroscopy, and X-ray fluorescence. Subsequently, the as-prepared nanoparticle was used to visualize latent fingermarks using a powder dusting method and to enhance the visualization of bloody fingermarks using a small particle reagent method on porous, semiporous, and nonporous substrates. Notably, the anti-ultraviolet property of this yellow nanoparticle was investigated. This intriguing property contributed to the detection of latent fingermarks on strong-fluorescence or multicolored surfaces in a negative-image manner. Moreover, two potential interaction mechanisms were proposed between hemoglobin and surfactants in bloody fingermark enhancement. This work showed that this rutile TiO 2 nanoparticle doped with Cr and Sb is a versatile eikonogen for the rapid visualization of latent and bloody fingermarks and has high sensitivity, selectivity, contrast as well as wide applicability compared with traditional developing methods.
研究领域:
法医学
·
材料科学
·
纳米技术
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Yunfeng Bai,Xiaoxuan Fan,Guoning Chen,Zhilong Zhao
Uric acid (UA) level is an important physiological indicator of the human body, which abnormality can lead to a series of diseases. Therefore, the immediate detection of uric acid concentration has broad application prospects. Commonly used methods for the analysis of uric acid include chromatography, high-performance capillary electrophoresis and electrochemical methods. However, these methods have the disadvantages of cumbersome sample pre-treatment, high cost, time-consuming, and the need for experimental instruments and professional operators, which are extremely unfavorable to the detection of uric acid and the diagnosis of related diseases in resource-limited areas. In this study, a portable visualization method was developed for the detection of uric acid by hydrogen peroxide (H2O2) test strips. Uric acid enzyme specifically catalyzes the oxidation of uric acid to produce H2O2, which causes a significant change in the color of the H2O2 test strip. The response has good linearity in the range of 1 ~ 50 μg mL-1. Thus, it provides a simple, rapid, and cost-effective visualized bioassay for uric acid.
研究领域:
生物化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Mingming Zhao,Huizi Lu,Zhiheng You,Huayun Chen,Xiao Wang,Yaqing Zhang,Yixian Wang
Developing a colorimetry-based artificial scent screening system (i.e., an olfactory visual sensing system) with high sensitivity and accurate pattern recognition for detecting fruit ripeness remains challenging. In this work, we construct a flexible dye/CelluMOFs-based sensor array with improved sensitivity for on-site detection of characteristic gases of fruits and integrate a densely connected convolutional network (DenseNet) into the sensor array, enabling it to recognize unique scent fingerprints and categorize the ripeness of fruits. In the system, CelluMOFs are synthesized through in situ growth of γ-cyclodextrin metal–organic frameworks (γ-CD-MOFs) on flexible fiber filter paper to fabricate a uniform, flexible and porous dye/CelluMOFs sensitive membrane. Compared to the pristine filter paper, the CelluMOFs exhibit increased porosity with a 62 times higher specific surface area and a 3-fold increase in dye loading capacity after 12 h of adsorption. The prepared dye/CelluMOFs sensing film shows outstanding mechanical and detection stability with negligible deviation after 100 cycles of rubbing. The colorimetric visualization arrays with multiple colorimetric dye/CelluMOFs chips, enable the sensitive recognition and detection of nine kinds of characteristic fruit odors and achieve a high response at 8–1500 ppm of trans-2-hexenal, showcasing remarkably low gas detection thresholds. On the basis of the ppm-level limit of detection with high sensitivity, the fabricated colorimetric sensor arrays are typically used for in situ assessment of fruit ripeness by integrating DenseNet. This approach achieves a satisfactory classification accuracy of 99.09% on the validation set, enabling high-precision prediction of fruit ripeness levels.
研究领域:
食品科学
·
材料科学
·
人工智能
✓ 已验证
产品关联
DOI
PubMed
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2021
·
IF 3.975
Xiaozhen Xiao,Chao Zhang,Lishan Chen,Liqiong Liao
For fast and visual detection of multiple metal ions , herein, a re-usable colorimetric polymeric gel with spiropyran moiety was fabricated. Under ultraviolet light, the spiropyran (SP) moiety could isomerize into the colored merocyanine (MC) isomer and chelate with divalent metal ion (M 2+ ) and form MC-M 2+ complexes. Chelated with different metal ions (Ca 2+ , Co 2+ , Zn 2+ , and Cu 2+ ions), the UV–Vis spectra of the gels could display the specific colorimetric response. When the metal-ions chelated gel was irradiated with Vis light, ions dissociated from MC and released from gel due to the MC moiety isomerized to the SP form. Furthermore, after 7 cycles of alternative UV–Vis irradiations, the gel showed excellent reversible chelation/dissociation of metal ion. The competitive tendency of metal ions to chelate with gel was as following: Cu 2+ > Co 2+ ≈ Zn 2+ > Ca 2+ , which was in accordance with the density functional theory modeling. Using ionoprinting technology, the gel could achieve repeatability simultaneous detection of multiple metal ions. The light-triggered metal ion chelation/dissociation capability of the gel could be harnessed to construct reusable onsite platform for visual, fast, easy detection of multiple metal ions.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Shuai Han,Liang Yang,Zhigang Wen,Suyun Chu,Mei Wang,Zhenyang Wang,Changlong Jiang
Residues in animal food and drinking water caused by the abuse of antibiotics lead to cell resistance and many chronic diseases in the human body. Therefore, it has become an inevitable trend to develop a fast, easy-to-use, on-site/real-time visualization method for the detection of antibiotics. Herein, we report a dual-response ratiometric fluorescence sensor which is fabricated by chelating europium ions (Eu 3+ ) onto cadmium telluride quantum dots (CdTe QDs) for real-time and visible detection of tetracycline (TC). With the TC addition, the fluorescence of probe can be seen by the naked eye, from green to yellow and finally to red, exhibiting a dosage-sensitive and broad-chromatic detection strategy for TC. The fluorescence intensity ratio of I 616 /I 512 of Eu/CdTe QDs sensor displays a good linear relation to TC concentrations in the range of 0–80 μM with a limit of detection (LOD) of 2.2 nM. In addition, the sensor can visually detect 200 nM TC in actual samples, which is lower than the maximum residue limit (MRL) of the safety standard. The methodology reported here opens a window toward the real applications of fluorescent and shows the wide applicability in pursuing the concepts simple, rapid, visual, and real-time for food safety and environmental protection.
研究领域:
分析化学
·
食品安全
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Hongyan Zhang,Ling Lin,Xiaoxue Zeng,Yajuan Ruan,Yongning Wu,Minggui Lin,Ye He,FengFu Fu
We herein developed a novel biosensor for the visual detection of trace uranyl ion ( UO 2 2 + ) in aqueous environment with high sensitivity and specificity by using DNAzyme-functionalized magnetic beads (MBs) for UO 2 2 + recognition and gold nano-particles (AuNPs)-based enzymatic catalysis oxidation of TMB (3,3′,5,5′-tetramethylbenzidine sulfate) for signal generation. The utilization of MBs facilitates the magnetic separation and collection of sensing system from complex sample solution, which leads to more convenient experimental operation and more strong resistibility of the biosensor to the matrix of sample, and the utilization of AuNPs-based enzymatic catalysis amplification greatly improved the sensitivity of the biosensor. Compared with the previous DNAzyme-based UO 2 2 + sensors, the proposed biosensor has outstanding advantages such as relative high sensitivity and specificity, operation convenience, low cost and more strong resistibility to the matrix of sample. It can be used to detect as low as 0.02 ppb (74 pM) of UO 2 2 + in aqueous environment by only naked-eye observation and 1.89 ppt (7.0 pM) of UO 2 2 + by UV–visible spectrophotometer with a recovery of 93–99% and a RSD≤5.0% ( n =6) within 3 h. Especially, the visual detection limit of 0.02 ppb (74 pM) is much lower than the maximum allowable level of UO 2 2 + (130 nM) in the drinking water defined by the U.S. Environmental Protection Agency (EPA), indicating that our method meets the requirement of rapid and on-site detection of UO 2 2 + in the aqueous environment by only naked-eye observation.
研究领域:
环境科学
·
生物传感
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Miao Yu,Yang Lu,Zhenjiang Tan
In this work, a novel and facile ratiometric fluorescence probe was prepared for the visual detection of dopamine (DA). In this detection system, red-emission CdTe@SiO 2 (r-QDs@SiO 2 ) was used as steady core of the probe and inverse microemulsion method was applied to synthesize uniform r-QDs@SiO 2 , this step could protect CdTe from contacting with human skin directly. Polydopamine (PDA) acted as response signal to detect DA, a very handy method which just combined polyethyleneimine (PEI) with DA together to synthesize PDA, this way for synthesis of PDA was much time-saving and non-toxic than any other methods. Differently from traditional analysis processes, the products of this experiment were also the analysis substances in final. Under optimum measurement conditions, the dual-emission ratiometric fluorescence probe was used for detections of DA in a concentration ranged from 10 μM to 80 μM with a detection limit of 0.12 μM, with addition of DA the color of the probe changed from red to green watched by naked eyes. In addition, the developed probe was also used for detections of DA in human serum samples successfully. This study provides a simple, time-saving and non-toxic approach for detections of DA without the requirement of complex equipment.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Hao Hu,Jinyi Zhang,Yu Ding,Xinfeng Zhang,Kailai Xu,Xiandeng Hou,Peng Wu
Singlet oxygen (1O2), generated via photosensitization, has been proved to oxidize chromogenic substrates with neither H2O2 oxidation nor enzyme (horseradish peroxidase, HRP) catalysis. Of the various methods for modulation of the 1O2 generation, DNA-controlled photosensitization received great attention. Therefore, integration of the formation/deformation DNA structures with DNA-controlled photosensitization will be extremely appealing in visual biosensor developments. Here, the stable melamine-thymine complex was explored in combination with DNA-controlled photosensitization for visual detection of melamine. A T-rich single stand DNA was utilized as the recognition unit. Upon the formation of the T-M-T complex, double stand DNA was formed, which was ready for the binding of SYBR Green I and activated the photosensitization. Subsequent oxidation of TMB allowed visual detection of melamine in dairy products, with spike-recoveries ranging from 94% to 106%.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2013
·
IF 5.437
Liping Liang,Yijuan Long,Haijie Zhang,Qinlong Wang,Xiaoxiao Huang,Rui Zhu,Ping Teng,Xiliang Wang,Huzhi Zheng
Two complementary colors mixed in a proper proportion will produce a neutral color in the color theory. A novel colorimetric method on basis of the color complementarity principle has been well-established to detect recombinant prion protein (rPrP). We found that a colorless solution appeared after mixing orange CdTe quantum dots (QDs) with green-blue malachite green (MG) because of color complementarity. After the addition of rPrP into the mixed solution, the color changed from colorless to green-blue because rPrP could induce the aggregation of QDs, rapidly. And it could be observed by naked eyes. Based on this phenomenon, we developed a simple assay for visual detection of rPrP. At the same time, we obtained excellent correlation between absorption and concentrations of rPrP from 1 nmol L −1 to 78 nmol L −1 with the limit of detection of 0.3 nmol L −1 (3 σ ). Moreover, it can be applied to determine rPrP in human serum successfully. Importantly, this assay possesses the advantages of simplicity, rapidity, sensitivity, and selectivity, and shows the potential in the clinical diagnostic test of early prion disease and provides the possibility of preventing the spread of prion diseases.
研究领域:
生物化学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xueyan Yun,Wenjin Chen,Jiatao Zhang,Tungalag Dong
Chilled meat is subject to deterioration by various factors during storage and distribution. Therefore, it is very important to monitor the quality of meat in real time. This study aims at preparing a natural, low-cost indicating microsphere to visualize the freshness of meat by the combination of sodium alginate (SA) and chitosan with 0–10 wt% anthocyanins derived from chokeberry as a colorant using ionic gelation method. Size-controlled porous SA microspheres with were further constructed by freeze-drying and their physicochemical properties were characterized by SEM, FTIR , DSC , and XRD. Results showed that microspheres with 1 wt% anthocyanin showed good responsiveness to different concentrations of ammonia and were able to effectively identify the freshness of chilled meat by color change. Principal component analysis showed that the color difference of the porous microspheres was highly significantly correlated with pH, TVB-N, total plate count and thiobarbituric acid active substance ( p < 0.01), suggesting a visible satisfactory capability of the microspheres to identify the spoilage in pork. Principal component analysis showed that the color difference of the porous microspheres was highly significantly correlated with pH, TVB-N, total plate count and thiobarbituric acid active substance ( P < 0.01), suggesting a visible satisfactory capability of the microspheres to identify the spoilage in pork.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）