JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2021
·
IF 4.291
Shenghong Yang,Xin Wang,Enshou Li,Xiaoyang Liu,Jinshuang Hu,Jian Liu
The environmental risk of artificial synthetic dyes sparked interest in exploring high-security fluorescent materials for suitable analysis applications. Chlorophyll-based fluorescent material is a good candidate but subjects to inferior dispersibility and low fluorescence efficiency in water. In this work, we successfully prepared a water-dispersible chlorophyll-based fluorescent material through simple solvent extraction of willow seeds. Compared to other conventional sources of chlorophyll, such as willow leaves, metasequoia, and green algae, only the chlorophyll-based material from willow seeds (C-WS) can retain good near-infrared fluorescence performance with an emission wavelength of 677 nm in the water phase, which can promote the practical application of chlorophyll-based fluorescent materials. An “on-off-on” sensing system was constructed based on C-WS for the highly sensitive and selective detection of Cu 2+ and biothiols in aqueous solution. The limit of detection for Cu 2+ and GSH can reach 1.2 nM and 92 nM, respectively. Furthermore, the proposed system was successfully used in the detection of Cu 2+ in real water samples and biothiols in food samples, and successfully applied for the visualization of intracellular Cu 2+ and biothiols in Hela cells.
研究领域:
分析化学
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环境科学
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生物医学
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2024
·
IF 6.6
BeiBei Lin,Peizhen Lin,Xiaobi Zhang,Yingyi Liao,Yinfei Yu,Xuegu Xu,Xiaoling Wang
Background Developing carrier-free nanomedicines via self-assembly of two antitumor drug molecules is a potential strategy for enhancing the combination treatment of tumors. Similarly, conventional chemotherapy combined with photodynamic therapy may synergistically improve the antitumor effect while minimizing the adverse reactions associated with antitumor treatment. Hyaluronic acid (HA) can bind to overexpressed HA receptors on the tumor cell surface, increasing cell internalization and resulting in good tumor-targeting properties.Methods Herein, we propose an easy and environmentally friendly approach for fabricating a novel formulation of carrier-free pure nanodrugs through self-assembly of the antitumor drug doxorubicin (DOX) and the photosensitizer chlorin e6 (Ce6), followed by sequential modification with HA to achieve simultaneous and targeted photodynamic therapy and chemotherapy.Results The fabricated HA-DOX-Ce6 nanoparticles (NPs) exhibited enhanced drug loading and stability while possessing the properties of near-infrared fluorescence imaging, self-targeting drug delivery, and combined photodynamic therapy and chemotherapy.Conclusions HA-DOX-Ce6 NPs, along with laser irradiation, effectively suppressed tumor development both in vitro and in vivo with minimal side effects. Overall, this biomaterial-based, carrier-free drug delivery system, in combination with cotargeted therapies, has shown potential for considerable improvements in treatment outcomes and patient prognosis.
研究领域:
肿瘤学
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药剂学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
OPTICS AND LASER TECHNOLOGY
·
2024
·
IF 4.6
Changping Xu,Yangyuan Zhang,Yangbo Bai,Yinping Miao
Sodium copper chlorophyllin (SCC) is a widely used food coloring which is natural and stable, but excessive intake can lead to hepatotoxicity and neurotoxicity. In this paper, a highly sensitive Fabry–Perot sensing technique for the detection of SCC is proposed based on its photothermal properties. The suggested sensor is constructed of a single-mode optical fiber encapsulated in a fiber capillary forming a Fabry–Perot cavity. The experimental results showed that the sensitivity of the SCC sensor was significantly improved up to 9.128 nm/(mg/L) under 405 nm laser irradiation with a detection limit of 0.00219 (mg/kg), which is three orders of magnitude lower than that of conventional high-performance liquid chromatography (HPLC). The sensor has a compact form and an integrated all fiber structure, which has potential applications in food safety monitoring, drug manufacturing and illness diagnostics.
研究领域:
食品安全
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光学传感
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毒理学
✓ 已验证
产品关联
DOI
高影响因子
BioNanoScience
·
2025
·Vol. 15
·期 2
·1-12
·
IF 3.2
Liang Tisong,Jing Pu
The poor stability of chlorophyll has inhibited its application in food industry. Our previous study showed that the stability of chlorophyll can be enhanced by incorporating with sodium caseinate (NaCas). This present work further explored the characteristics of NaCas–chlorophyll dispersion in simulated gastrointestinal digestion and its inhibitory effects on human epithelial colorectal adenocarcinoma cells (Caco-2 cells). Results indicated that the gastrointestinal digestion resulted in alterations to the physical appearance of the NaCas–chlorophyll dispersion. Fourier-transform infrared spectroscopy illustrated that the second structure of NaCas underwent alterations during gastrointestinal digestion. Morphological observation revealed that the digested NaCas–chlorophyll dispersion exhibited granular morphology with smaller particle size. The Caco-2 cells exposed to digested NaCas–chlorophyll dispersion exhibited decreased viability and destroyed mitochondrial structure, which may be related to the enhanced absorptivity of the NaCas–chlorophyll dispersion following gastrointestinal digestion. These findings provided theoretical basis for understanding the characteristics changes and biological activity of NaCas–chlorophyll dispersion during gastrointestinal digestion.
研究领域:
食品科学
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生物化学
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营养学
✓ 已验证
产品关联
DOI
高影响因子
PLoS One
·
2020
·Vol. 15
·期 5
·e0232750
·
IF 2.74
Xueqing He,Pei You,Yunfu Sun
The rare earth element lanthanum (La) has been proven to be beneficial for plant growth with a low concentration, and abscisic acid (ABA) which is a plant hormone also can regulate plant growth. In the present study, we investigated the germination and seedling growth of switchgrass ( Panicum virgatum L.) under La (10 μM), ABA (10 μM) and La + ABA treatments. The results showed that La, ABA and La + ABA treatments could not significantly affect the germination and shoot length as compared to the control ( P >0.05). However, La treatment increased the root activity and chlorophyll content, and ABA treatment enhanced root length and root activity ( P <0.05). La + ABA treatments demonstrated that La could not significantly alleviate the promotion of ABA in root length, while ABA reversed the increase of chlorophyll content caused by La. The coregulation of La and ABA on chlorophyll content was further explored by in vitro experiments and quantum chemical calculations. In vitro experiments revealed that La, ABA, and La + ABA treatments reduced the absorbance of chlorophyll, and quantum chemical calculations indicated that the reduction of absorbance was caused by the reactions between La, ABA and chlorophyll. In vivo and in vitro experiments, together with quantum chemical calculations, demonstrated that both ABA and La could stimulate the production of chlorophyll, while they also could react with chlorophyll to produce La-monochlorophyll, La-bischlorophyll, and ABA adsorbed chlorophyll, which had lower absorbance. La + ABA treatment significantly decreased the chlorophyll content in vivo. This phenomenon was due to the fact that La and ABA formed LaABA compound, which markedly reduced the concentrations of ABA and La, and the effect of promoting chlorophyll production was overcome by the effect of reducing chlorophyll absorbance.
研究领域:
植物科学
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农学
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量子化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYCOLOGIA
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2015
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IF 1.915
Rui Liu,Xiaofei Ran,Fang Bai,Jinzhu Xu,Songqi Yang,Junqiong Shi,Zhongxing Wu
In order to investigate the toxicity targets of the secondary metabolite from aquatic plants, N-phenyl-2-naphthylamine, on Cylindrospermopsis raciborskii photosynthetic system II (PSII), we determined chlorophyll a (Chl a), specific growth rate (μ−d), photosynthetic rapid light curves and polyphasic Chl a fluorescence transients following exposure to N-phenyl-2-naphthylamine. The results showed significant decreases in Chl a following 48 h at high concentration (0.50 mg l−1) exposure to N-phenyl-2-naphthylamine. The PSII electron transfer rate and Chl a fluorescence transients appeared to be affected after the cells were exposed to concentrations of 0.50, 1.00 and 2.00 mg l−1. Exposure to higher concentration of N-phenyl-2-naphthylamine (> 0.50 mg l−1) mainly depressed the density of reaction centers (RC/CS0) and quantum energy flux ratios (ϕP0 and ϕE0) but increased the absorption flux per RC (ABS/RC), dissipated energy flux per RC (DI0/RC) and the average redox state of QA−/QA in the time span from 0 to tFmax (Sm/tFmax). However, exposure to low concentration (≤ 0.5 mg l−1) mainly inhibited the trapping energy fluxes per reaction center (TR0/RC), electron transport energy fluxes per reaction center (ET0/RC) and energy flux ratio (ψ0). This suggested that the donor and the acceptor sides of PSII were targets of N-phenyl-2-naphthylamine in C. raciborskii. Additionally, our results indicated that the toxicity target at low concentrations of N-phenyl-2-naphthylamine was at each step of electron transport between QA− and the Cyt b6/f complex; whereas, the reduction of QA was the potential target at high concentrations. We suggest that N-phenyl-2-naphthylamine may be a potential allelochemical to control harmful cyanobacterial blooms.
研究领域:
水生生物学
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环境科学
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植物生理学
✓ 已验证
产品关联
DOI
高影响因子
IRANIAN POLYMER JOURNAL
·
2017
·Vol. 26
·期 3
·205-211
·
IF 1.422
Zhou Min-Jie,He Fang,Wu Hu,Wang Guo-Xiang,Liu Li-Chao,Xu Wenyuan
Fe-mediated photo-induced atom transfer radical polymerization (ATRP) of methyl methacrylate was studied with chlorophyll A as photoredox catalyst in the absence of ligands. This is the first report on photo-induced and FeBr 3 -mediated ATRP. A low-cost and environmentally friendly ATRP is provided in this system. A good linear semi-logarithmic plot of polymerization kinetics is the first-order time conversion, and narrow molecular weight distributions explain the well-controlled behavior. The measured number average molecular weight, M
n,GPC (determined by gel permeation chromatography), is increased with monomer conversion, and it is in good agreement with the theoretical values ( M
n,th ). The effect of FeBr 3 is studied with respect to the polymerization. The values of the resultant PMMA increased with reduced FeBr 3 concentration. In this system, the effect of periodic light/off process has been investigated through chain growth instantly turned on and off in response to the visible light. The polymerization was controllable even though the concentration of Fe-based catalyst was dropped to 4 ppm. The chain-end group having a functionality in the synthesized poly(methyl methacrylate) was characterized by nuclear magnetic resonance spectrometry ( 1 H NMR). However, chain extension experiments show reserved chain-end functionality in the synthesized polymers and further confirm the “living” feature of the Fe-mediated photo-induced atom transfer radical polymerization.
研究领域:
材料科学
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高分子化学
✓ 已验证
产品关联
DOI
高影响因子
Arabian Journal of Chemistry
·
2023
·
IF 6
Hong Shen,Changwei Zhang,Hao zhou,Chuan Li,Hua Yuan,Jianxin Jiang,Chengzhang Wang
Chlorophyll compounds (Chls) from Ginkgo biloba leaves (GBL) are non-toxic natural pigments characterized by highly conjugated molecules and special spectra, making them ideally potential photosensitizers (PSs) in photodynamics therapy (PDT). In this study, 4 Chls were isolated from Ginkgo biloba polyprenols (GBPPs) wastes by using silica gel column separation and high performance liquid chromatography (HPLC). In addition, two new chlorophyll derivatives were successfully synthesized with chlorin e6 as the primary raw material. To evaluate and identify superior chlorophyll PSs, Density Functional Theory (DFT) and Density Functional Theory (TD-DFT)were employed to decode the microscopic and spectral properties of Ginkgo biloba Chls (GBChls). The spectral absorption, optical stability and reactive oxygen species (ROS) production of GBChls were further analyzed through comparisons of their frontier molecular orbital layout, charge distribution and excitation energy properties. Our results show that about 63 % absorption in the Q band for 14 GBChls comes from the π⇢π* transition from the highest occupied orbital (HOMO) to the lowest unoccupied orbital (LUMO), indicating the favorable delocalization effect of these GBChls. In particular, those GBChls with the long chain of the phytyl group exhibited higher total electron energy and thus could significantly increase the light stability. However, the large spatial volume and hydrophobicity of these compounds with the phytyl group chain was unfavorable for cellular uptake, leading to low ROS in cells. The electronegativity of porphin macrocycles in GBChls positively correlates with the intracellular ROS production rate, meaning that the former likely promotes the energy transfer between PSs and molecular oxygen during type II photoreaction. Among the GBChls, chlorin e6 demonstrated the highest ROS yields in DMF, while purpurin 18 compounds displayed long-wavelength absorption (698 nm), strong Q-band absorption, good optical stability, and high intracellular ROS production. Our findings highlight that chlorin e6 and purpurin 18 are most potential PSs anticancer drugs and offer new research perspectives for the processing and utilization of GBL resources.
研究领域:
植物化学
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药物化学
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光动力疗法
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Yuting Sun,Jian Jiang,Yu-Ting Xu,Yuhong He,Ziyan Liu,Aijun Li,Haotong Wei,Hai Xu,Hitoshi Tamiaki,Shin-ichi Sasaki,Xiao-Feng Wang
Photodetectors, with high sensitivity and broad spectral response, drive the advancements in optical imaging technology. In this work, utilizing the absorption differences of chlorophyll-a derivative (Chl) and [6,6]-phenyl-C 61 -butyric acid methyl ester, two distinct structures of multifunctional organic photodetectors (OPDs) are fabricated, namely, bulk heterojunction (BHJ) and quasiplanar heterojunction (Q-PHJ). Chl, by modulating its doping concentration, served as both an active layer and an electron trap, enabling the BHJ OPD to exhibit a photomultiplication effect, and allowing the Q-PHJ OPD to achieve improved response speed (0.12/0.13 μs) and specific detectivity (2.20 × 10 13 Jones). In addition, the visible image-sensing function has been demonstrated in the OPD arrays. Based on the bidirectional current response behavior of Q-PHJ OPD under bias voltage regulation, the moving detection function is implemented by combining it with background subtraction, extracting the moving objects from the image stream.
研究领域:
材料科学
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光学工程
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2023
·Vol. 35
·期 47
·2302973
·
IF 29.4
Dongjin Xie,Mei Zu,Mingyang Li,Dongqing Liu,Zi Wang,Qingwen Li,Haifeng Cheng
The unmet spectral mimicry of foliar green in camouflage materials is hampered by the lack of colorants with similar spectral properties to chlorophyll, resulting in substantial risks of exposure from hyperspectral target detection. By drawing inspiration from leaf chromogenesis, a microcapsule colorant with a chloroplast-like structure and chlorophyll-like absorption is developed, and a generic bilayer coating is designed to provide high spectral similarity to leaves with different growth stages, seasons, and species. Specifically, the microcapsule colorant preserves the monomeric absorption of the internal phthalocyanine and features the manufacturability of conventional pigments, such as amenability to painting and patterning, and compatibility to different substrates. The pigmented artificial leaves successfully deceive the hyperspectral classification algorithm in a foliar background, and outperforming the state-of-art spectral simulation materials. This coloration strategy expands the knowledge base of the spectral fine tuning of composite colorants, which are essential for their application in spectral-resolved optical materials.
研究领域:
材料科学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Marine Drugs
·
2024
·Vol. 22
·期 9
·387
·
IF 4.9
Chunyan Zheng,Jie Yang,Yunting Wang,Waqas Ahmed,Amir Khan,Jiannan Li,Jiechang Weng,Sajid Mehmood,Weidong Li
This study investigated the effects of herbicide exposure onNaviculasp. (MASCC-0035) algae, focusing on growth density, chlorophyll content, antioxidant system, and lipid metabolism.Naviculacultures were exposed to different concentrations of atrazine (ATZ), glyphosate (Gly), and acetochlor (ACT) for 96 h. Results showed a significant decrease in cell numbers, with higher herbicide concentrations having the most noticeable impacts. For instance, Gly-G2 had reduced cell populations by 21.00% at 96 h. Chlorophyll content varied, with Gly having a greater impact on chlorophyll a compared to ATZ and ACT. Herbicide exposure also affected the antioxidant system, altering levels of soluble sugar, soluble protein, and reactive oxygen species (ROS). Higher herbicide rates increased soluble sugar content (e.g., ATZ, Gly, and ACT-G2 had increased by 14.03%, 19.88%, and 19.83%, respectively, at 72 h) but decreased soluble protein content, notably in Gly-G2 by 11.40%, indicating cellular stress. Lipid metabolism analysis revealed complex responses, with changes in free proline, fatty acids, and lipase content, each herbicide exerting distinct effects. These findings highlight the multifaceted impacts of herbicide exposure onNaviculaalgae, emphasizing the need for further research to understand ecological implications and develop mitigation strategies for aquatic ecosystems.
研究领域:
环境科学
·
植物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSYSTEMS ENGINEERING
·
2021
·
IF 4.123
Jiyong Shi,Yueying Wang,Zhihua Li,Xiaowei Huang,Tingting Shen,Xiaobo Zou
Nitrogen (N), magnesium (Mg), and potassium (K) deficiency all result in the decrease of chlorophyll density (CH.D) in cucumber leaf, but it is difficult to differentiate N/Mg/K-deficient leaves using CH.D. New nutrient indicators based on CH.D distribution features of the whole cucumber leaf were proposed to simultaneously diagnose N/Mg/K-deficient cucumber plants. CH.D distribution map in N/Mg/K-deficient and control leaves were determined non-destructively using hyperspectral imaging with the wavelength range of 430–960 nm. Compared with the CH.D distribution map of control group leaves, a decrease in CH.D could be observed on the whole area of N-deficient leaves, Mg-deficient leaves presented pixels with low CH.D between main leaf vein, and pixels with low CH.D at the border of K-deficient leaves. Distribution features were extracted from the CH.D distribution map and were employed to diagnose N/Mg/K-deficient leaves simultaneously, with the diagnostics rates of 96.67%. The results indicated that CH.D distribution features can realize the simultaneous diagnosing in the early stage of N/Mg/K deficiency and timely guidance of top dressing is of great significance to increase yield.
研究领域:
植物营养学
·
农业遥感
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）