Environmental Science-Nano
·
2020
·Vol. 7
·期 11
·3526-3535
·
IF 7.683
Wenchao Li,Ruoyu Fan,Hongjian Zhou,Yinfeng Zhu,Xu Zheng,Mengyu Tang,Xiaosi Wu,Chengzhong Yu,Guozhong Wang
Compared with root nitrogen fertilizers, foliar nitrogen fertilizers (FNFs) have been widely used for crop growth due to their high fertilizer efficiency and high utilization rate, especially when the crops are deficient in nitrogen. Owing to the lotus leaf effect intrinsic to the leaf surface of crops, however, the majority of FNFs will easily slip from the leaf surface and be discharged into the soil environment by rainwater scouring, causing inferior utilization rates and serious soil pollution. Therefore, it is of paramount importance to remedy the adhesion capacity of FNFs on the leaf surface of crops for improving the utilization rate of FNFs. In this study, three kinds of micro–nanostructured silica spheres (e.g., solid silica spheres (S-Si), hollow silica spheres (H-Si) and sea urchin-like micro–nanostructured hollow silica spheres (SUH-Si)) with similar particle diameters (∼500 nm), different surface roughness, and diverse surface morphologies were utilized as carrier materials to load a nitrogen fertilizer to improve the utilization rate of FNFs on plant leaves. As a result, SUH-Si with the highest surface roughness among the three carriers leads to a change of adhesion capacity of the FNF on the surface of plant leaves, thus resulting in a superior infiltration effect of the nitrogen fertilizer. Compared with that of traditional FNFs, the adhesion capacity of the FNF with SUH-Si on peanut leaves and maize leaves was increased by 5.9 times and 2.2 times, respectively, resulting in a 2.29 times improved utilization rate of the FNF due to SUH-Si. Finally, contact angle measurements and microstructure analysis, as well as the calculation of interaction forces between the silica spheres and plant leaf surface, provided in-depth understanding to improve the adhesion capacity of foliar nitrogen fertilizers by surface roughness engineering of silica spheres. Our study would be helpful for developing FNFs with high efficiency and utilization rates by surface roughness engineering.
研究领域:
植物学
·
农业科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED MATERIALS
·
2023
·Vol. 35
·期 47
·2302973
·
IF 29.4
Dongjin Xie,Mei Zu,Mingyang Li,Dongqing Liu,Zi Wang,Qingwen Li,Haifeng Cheng
The unmet spectral mimicry of foliar green in camouflage materials is hampered by the lack of colorants with similar spectral properties to chlorophyll, resulting in substantial risks of exposure from hyperspectral target detection. By drawing inspiration from leaf chromogenesis, a microcapsule colorant with a chloroplast-like structure and chlorophyll-like absorption is developed, and a generic bilayer coating is designed to provide high spectral similarity to leaves with different growth stages, seasons, and species. Specifically, the microcapsule colorant preserves the monomeric absorption of the internal phthalocyanine and features the manufacturability of conventional pigments, such as amenability to painting and patterning, and compatibility to different substrates. The pigmented artificial leaves successfully deceive the hyperspectral classification algorithm in a foliar background, and outperforming the state-of-art spectral simulation materials. This coloration strategy expands the knowledge base of the spectral fine tuning of composite colorants, which are essential for their application in spectral-resolved optical materials.
研究领域:
材料科学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSYSTEMS ENGINEERING
·
2021
·
IF 4.123
Jiyong Shi,Yueying Wang,Zhihua Li,Xiaowei Huang,Tingting Shen,Xiaobo Zou
Nitrogen (N), magnesium (Mg), and potassium (K) deficiency all result in the decrease of chlorophyll density (CH.D) in cucumber leaf, but it is difficult to differentiate N/Mg/K-deficient leaves using CH.D. New nutrient indicators based on CH.D distribution features of the whole cucumber leaf were proposed to simultaneously diagnose N/Mg/K-deficient cucumber plants. CH.D distribution map in N/Mg/K-deficient and control leaves were determined non-destructively using hyperspectral imaging with the wavelength range of 430–960 nm. Compared with the CH.D distribution map of control group leaves, a decrease in CH.D could be observed on the whole area of N-deficient leaves, Mg-deficient leaves presented pixels with low CH.D between main leaf vein, and pixels with low CH.D at the border of K-deficient leaves. Distribution features were extracted from the CH.D distribution map and were employed to diagnose N/Mg/K-deficient leaves simultaneously, with the diagnostics rates of 96.67%. The results indicated that CH.D distribution features can realize the simultaneous diagnosing in the early stage of N/Mg/K deficiency and timely guidance of top dressing is of great significance to increase yield.
研究领域:
植物营养学
·
农业遥感
✓ 已验证
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2020
·Vol. 104
·期 9
·3947-3957
·
IF 3.53
Qu Yuejun,Yang Yue,Du Renjie,Zhao Min
A sensing methodology that combines Au, tobacco mosaic virus (TMV), and folic acid for selective, sensitive, and colorimetric detection of tumor cells based on the peroxidase-like activity was reported in this study. Gold nanowires with a high aspect ratio were synthesized using TMV as a template. Au@TMV nanowire (AT) complex was obtained with diameter of 4 nm and length between 200 and 300 nm. In addition, since TMV was biocompatible and had many amino and carboxyl groups on its surface, AT was conjugated by folate to form a folic acid (FA)–conjugated AT composite (ATF) and tested by FTIR measurements. Furthermore, the peroxidase-like properties were studied and the optimal conditions for mimic enzyme activity were optimized. Finally, HeLa and other tumor cells expressed excessive receptors of folate on the surface, which can specifically bind to folic acid. As the specific binding of ATF with HeLa cells, the peroxidase properties of ATF were used for detection of cancer cells (Scheme 1). The cancer cells were detected not only qualitatively but also quantitatively. In this study, as low as 2000 cancer cells/mL could be detected using the current method.
研究领域:
生物化学
·
纳米生物学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Wenyang Tao,Haibo Pan,He Jiang,Mengting Wang,Xingqian Ye,Shiguo chen
Proanthocyanidins is flavan-3-ol polymers with many activities which attracted a lot of attention. However, most of the proanthocyanidins come from fruits and seeds, resulting in higher costs. The extraction of proanthocyanidins from leaves that were trimmed as wastes from fruit trees is of good economic benefits. The proanthocyanidins in persimmon leaves and loquat leaves were extracted and purified. The purity of persimmon and loquat leaves were 85.33 ± 0.11% and 88.45 ± 0.96% with yield of 3.40% and 2.37% respectively. Detailed structure information was analyzed. Persimmon leaves proanthocyanidins mainly consist of catechin with B-type link along with a small portion of gallocatechin, catechin gallate and A-type link. Loquat leaves proanthocyanidins consist of catechin, gallocatechin, gallocatechin gallate and afzelechin with B-type link along with a small portion of A-type link. The α-amylase inhibition effect of the two leaves was analyzed. Persimmon leaves proanthocyanidins and loquat leaves proanthocyanidins were two mixed-type inhibitors to α-amylase.
研究领域:
生药学
·
生物化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Jiali Qian,Feifei Quan,Fengjiao Zhao,Chengxin Wu,Zhaoyan Wang,Lei Zhou
Herein, we utilized aconitic acid (AA) as the precursor for preparing carbon dots (AA-CDs) by a facile hydrothermal reaction , and the resulting AA-CDs exhibited bright blue fluorescence with an absolute quantum yield of 56.5% in aqueous solution. Preliminary experiments showed that the prepared AA-CDs without further surface passivation or modification could selectively interact with folic acid (FA), resulting in significant fluorescence quenching. Based on this, a sensitive method for FA analysis was developed with a detection limit of 40 nM (S/N = 3), which was then successfully applied for the detection of FA in food and pharmaceutical samples with the average recoveries of 95.0–105.3%. Furthermore, a weak fluorescent nanoprobe (FA-AA-CDs) was fabricated based on the conjugated interaction of FA and AA-CDs, and its feasibility was evaluated as a fluorescence turn-on nanoprobe for targeted imaging of cancer cells using Hela, SMMC-7721, and A549 cells as models that expressed different levels of folate receptors (FRs) on the cell surface. The results exhibited that the fluorescence intensity of these cells was in accordance with their FRs expression levels, testifying its potential of FA-AA-CDs for targeted imaging of cancer cells. Subsequently, the internalization mechanism of FA-AA-CDs into cells was elucidated via receptor-mediated endocytosis using control experiments.
研究领域:
分析化学
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纳米医学
·
肿瘤学
·
分子影像
·
药物递送
·
生物传感
·
细胞生物学
✓ 已验证
DOI
高影响因子
PLANT GROWTH REGULATION
·
2025
·
IF 3.9
Ling Qingyan,Zhang Beihong,Xiao Zufei,Shen Yao,Jiang Junfei,Xu Zhiwei,Liu Qingqing,Xiao Changlong,Zeng Rong,Liu Yuanqiu,Jin Zhinong
Citral-type Camphora officinarum ( C. officinarum ) exhibits high economic value due to its branch and leaf essential oil enriched with citral, yet the low natural concentration of this compound necessitates urgent strategies to enhance yield and quality. Methyl jasmonate (MeJA), a recognized safe elicitor, stimulates aromatic compound accumulation in plants and promotes monoterpene synthesis in C. officinarum suspension cells. However, it remained unclear whether foliar MeJA application enhances monoterpene biosynthesis in C. officinarum . This study evaluated the effects of near-harvest foliar MeJA application at gradient concentrations 0 μmol/L (control, M0), 100 μmol/L (M100), 250 μmol/L (M250), and 500 μmol/L (M500) on growth, oil gland morphology, and essential oil composition in citral-type C. officinarum . The results demonstrated that: M100 treatment significantly increased plant height, branch biomass, leaf biomass, and total biomass compared to the control M0. M250 treatment induced structural modifications in oil cells, enlarging their diameter and increasing density, resulting in an 11.83% increase in essential oil yield per unit fresh leaf weight. Both M100 and M250 treatments enhanced citral content by 3.6% and 3.31%, respectively, while total essential oil production surged by 12.31% (M100) and 15.46% (M250). Principal component analysis (PCA) collectively demonstrated that 100–250 μmol/L MeJA (M100/M250) represented the optimal induction concentration for enhancing essential oil yield and promoting citral synthesis, whereas 500 μmol/L (M500) induced detrimental osmotic stress. These findings demonstrate that MeJA serves as a safe agricultural regulation approach for enhancing essential oil yield and citral content, offering valuable insights for other aromatic plant species.
研究领域:
植物生理学
·
农学
·
天然产物化学
·
植物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Ying Zhou,Rufang Deng,Xinlan Xu,Ziyin Yang
Linalool is abundant in tea leaves and contributes greatly to tea aroma. The two isomers of linalool, (R)-linalool and (S)-linalool, exist in tea leaves. Our study found that (R)-linalool was the minor isomer in nine of Camellia sinensis var. sinensis cultivars. The (R)-linalool synthase of tea plant CsRLIS was identified subsequently. It is a chloroplast-located protein and specifically catalyzes the formation of (R)-linalool in vitro and in vivo. CsRLIS was observed to be a stress-responsive gene and caused the accumulation of internal (R)-linalool during oolong tea manufacture, mechanical wounding, and insect attack. Further study demonstrated that the catalytic efficiency of CsRLIS was much lower than that of (S)-linalool synthase CsSLIS, which might explain the lower (R)-linalool proportion in C. sinensis var. sinensis cultivars. The relative expression levels of CsRLIS and CsSLIS may also affect the (R)-linalool proportions among C. sinensis var. sinensis cultivars. This information will help us understand differential distributions of chiral aroma compounds in tea.
研究领域:
植物学
·
分子生物学
·
芳香化合物代谢
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2018
·
IF 2.297
Yunfei Wang,Jin Ren,Yonghua Liu,Rong Liu,Liangliang Wang,Qinglan Yuan,Jianming He,Yamin Nie,Jing Xu,Jingmou Yu
In this study, novel folic acid modified succinylated gelatin (FA-SG) micelles were prepared and used as drug carrier in folate-receptor (FR) targeted antitumor therapy. FA-SG copolymer was synthesized and characterized by 1 H NMR and fluorescence spectroscopy. Doxorubicin-loaded FA-SG (DOX-FA-SG) micelles were prepared by a dialysis method. The shape of DOX-FA-SG micelles was almost spherical by transmission electron microscopy analysis. The mean diameter of DOX-FA-SG micelles was 277 nm , and the DOX-loading content was 12.4%. In vitro drug release of DOX-loaded micelles showed sustained release behavior for 168 h. DOX-FA-SG demonstrated significantly greater cellular uptake and cytotoxicity against FR-positive MCF-7 cells than the non-targeted DOX-loaded micelles. Importantly, DOX-FA-SG micelles exhibited higher antitumor activity in MCF-7 tumor-bearing mice than doxorubicin hydrochloride (DOX⋅HCl) and DOX-loaded micelles without FA modification. Taken together, FA-SG micelles could be an effective carrier for targeted drug delivery .
研究领域:
药剂学
·
药物递送系统
·
癌症研究
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Xiuhai Gan,Zhengxing Wang,Deyu Hu
To develop novel antiviral agents, some novel conjugates between ferulic acid and eugenol or isoeugenol were designed and synthesized by the link reaction. The antiviral activities of compounds were evaluated using the half leaf dead spot method. Bioassay results showed acceptable antiviral activities of some conjugates against the tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV). Compounds A9, A10, E1, and E4 showed remarkable curative, protective, and inactivating effects on TMV and CMV at 500 μg mL–1. Notably, these compounds exhibited excellent protective effects on TMV and CMV. The EC50 values of compounds A9, A10, E1, and E4 against TMV were 180.5, 169.5, 211.4, and 135.5 μg mL–1, respectively, and those against CMV were 210.5, 239.1, 218.4, and 178.6 μg mL–1, respectively, which were superior to those of ferulic acid (471.5 and 489.2 μg mL–1), eugenol (456.3 and 463.2 μg mL–1), isoeugenol (478.4 and 487.5 μg mL–1), and ningnanmycin (246.5 and 286.6 μg mL–1). Then, the antiviral mechanisms of compound E4 were investigated by determining defensive enzyme activities and multi-omics analysis. The results indicated that compound E4 resisted the virus infection by enhancing defensive responses via inducing the accumulation of secondary metabolites from the phenylpropanoid biosynthesis pathway in tobacco.
研究领域:
植物化学
·
病毒学
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2023
·Vol. 13
·期 1
·1-12
·
IF 4.6
Huang Huiming,Zou Hui,Lin Hongting,Dai Yimin,Lin Jiangbo
Plants with partial or complete loss of chlorophylls and other pigments are frequently occurring in nature but not commonly found. In the present study, we characterize a leaf color mutant ‘ arly01 ’ with an albino stripe in the middle of the leaf, which is an uncommon ornamental trait in Anoectochilus roxburghii . The albino “mutant” middle portion and green “normal” leaf parts were observed by transmission electron microscopy (TEM), and their pigment contents were determined. The mutant portion exhibited underdevelopment of plastids and had reduced chlorophyll and other pigment (carotenoid, anthocyanin, and flavonoid) content compared to the normal portion. Meanwhile, comparative transcript analysis and metabolic pathways mapping showed that a total of 599 differentially expressed genes were mapped to 78 KEGG pathways, most of which were down-regulated in the mutant portion. The five most affected metabolic pathways were determined to be oxidative phosphorylation, photosynthesis system, carbon fixation & starch and sucrose metabolism, porphyrin and chlorophyll metabolism, and flavonoid biosynthesis. Our findings suggested that the mutant ‘ arly01 ’ was a partial albinism of A. roxburghii , characterized by the underdevelopment of chloroplasts, low contents of photosynthetic and other color pigments, and a number of down-regulated genes and metabolites. With the emergence of ornamental A. roxburghii in southern China, ‘ arly01 ’ could become a popular cultivar due to its unique aesthetics.
研究领域:
植物学
·
遗传学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Chengxin Luan,Huan Wang,Qi Han,Xiaoyan Ma,Dagan Zhang,Yueshuang Xu,Baoan Chen,Minli Li,Yuanjin Zhao
Recovery of circulating tumor cells (CTCs) from cancer patients by an efficient CTCs capture and release method can greatly increase their application in diagnostics and treatment of cancers. In this paper, we presented a folic acid (FA)-functionalized hybrid photonic barcode for capture and release of CTCs. The hybrid photonic barcodes were formed by two nano-ordered parts, poly(ethylene glycol) diacrylate (PEGDA) inverse opal structure for sustaining integrity and methacrylated gelatin (GelMA) gel filler for conjugating FA molecules to mediate cell capture. The nano-ordered structures of the hybrid photonic barcodes not only increased contact area, but also decreased steric hindrance among FA molecules. Thus, the topographic interaction between the barcodes and CTCs was greatly enhanced. In addition, GelMA gel was soft and extracellular matrix (ECM) alike, which was beneficial to decrease impairment to CTCs during the CTCs-barcode interaction as well as preserving their viability. Demonstrated by four CTCs types, Hela (epithelial tissue, folate receptor positive, FR+), A02 (bone marrow, FR+), Raji (lymphoid tissue, FR+), and A549 (epithelial tissue, folate receptor negative, FR-), FR+ CTCs could be captured efficiently with reliability and specificity. The captured cells could be controllably released with high viability just by quick trypsinization. The whole processes were simple and efficient. These features indicated that the FA-functionalized hybrid photonic barcodes were promising for full recovery of CTCs from cancer patients, which was important for diagnosis and treatment of cancer.
研究领域:
生物医学工程
·
肿瘤学
·
材料科学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）