Bioresources and Bioprocessing
·
2022
·Vol. 9
·期 1
·1-16
·
IF 4.983
Sun Bing-Yao,Sui Hua-Lu,Liu Zi-Wei,Tao Xin-Yi,Gao Bei,Zhao Ming,Ma Yu-Shu,Zhao Jian,Liu Min,Wang Feng-Qing,Wei Dong-Zhi
Indirubin is a bisindole compound for the treatment of chronic myelocytic leukemia. Here, we presented a structure-guided method to improve the activity of a flavin-containing monooxygenase (bFMO) for the efficient production of indirubin in Escherichia coli. A flexible loop interlocked with the active pocket through a helix and the substrate tunnel rather than the active pocket in bFMO were identified to be two reconfigurable structures to improve its activity, resulting in K223R and N291T mutants with enhanced catalytic activity by 2.5- and 2.0-fold, respectively. A combined modification at the two regions (K223R/D317S) achieved a 6.6-fold improvement in catalytic efficiency (kcat/Km) due to enhancing π–π stacking interactions stabilization. Finally, an engineered E. coli strain was constructed by metabolic engineering, which could produce 860.7 mg/L (18 mg/L/h) indirubin, the highest yield ever reported. This work provides new insight into the redesign of FMOs to boost their activities and an efficient approach to produce indirubin.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Zunbin Duan,He Bian,Lijun Zhu,Daohong Xia
Efficient removal of thiophenic sulfides is important for the purification of fuel. Herein, 2-hydroxypropyl- β -cyclodextrin polymers (2-HP- β -CDPs) with micro-mesopores are synthesized using 2-HP- β -CD and tetrafluoroterephthalonitrile (TFTPN) for removing thiophenic sulfides. The cross-linking of 2-HP- β -CD and TFTPN units forms the mesopores, and the cyclodextrin cavity acts as the micropores. The 2-HP- β -CDP 1:4 (1:4 is the molar ratio of 2-HP- β -CD and TFTPN in preparation) possesses a surface area of 197.49 m 2 g –1 , and most of pores are at 1.09 nm of micropores and 6.06 nm of mesopores. The synergistic effect of the micro-mesopores promotes 2-HP- β -CDP 1:4 to remove the thiophenic sulfides in the order of benzo[ b ]thiophene > 4,6-dimethyldibenzothiophene ≈ dibenzothiophene > thiophene. The removal efficiency and rate using 2-HP- β -CDP 1:4 are better than those of CD-based and most traditional adsorbents that have been reported. Most of sulfides adsorbed by mesopores diffuse rapidly to micropores from the wide end of cyclodextrin and then are selectively captured by the cyclodextrin units in 2-HP- β -CDP 1:4 . These findings can guide the synthesis of CD-based polymers and the application of fuel purification.
✓ 已验证
产品关联
DOI
高影响因子
BRAIN BEHAVIOR AND IMMUNITY
·
2022
·
IF 19.227
Jing Sun,Yuhe Zhang,Yu Kong,Tao Ye,Qingxia Yu,Senthil Kumaran Satyanarayanan,Kuan-Pin Su,Jiaming Liu
Gut microbiota alterations might affect the development of Alzheimer’s disease (AD) through microbiota-derived metabolites. For example, microbiota-derived Indoles via tryptophan metabolism prevented Aβ accumulation and Tau hyperphosphorylation, restored synaptic plasticity, and then promoted the cognitive and behavioral ability of APP/PS1 mice. The imbalanced compositions of Indoles-producing bacteria with tryptophan deficiency were found in male APP/PS1 mice, but the molecular mechanisms remained unclear. Our current study revealed that Indoles (including indole, indole-3-acetic acid and indole-3-propionic acid) upregulated the production of aryl hydrocarbon receptor (AhR), inhibited the activation of the NF-κB signal pathway as well as the formation of the NLRP3 inflammasome, reduced the release of inflammatory cytokines, including TNF-α, IL-6, IL-1β and IL-18, alleviating the inflammatory response of APP/PS1 mice. These findings demonstrated the roles of Indoles-producing bacteria in activating the AhR pathway to regulate neuroinflammation of AD through gut microbiota-derived Indoles, which implied a novel way for AD treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOLOGICAL CONTROL
·
2022
·
IF 3.857
Jinhao Zhao,Minmin Liang,Zhongyan Wang,Yanyan Zhao,Jingli Cheng,Yongjun Du
Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae) is one of the most important parasitic natural enemies of Grapholita molesta (Busck) (Lepidoptera: Tortricidae) in peach and pear orchards. The artificial rearing T. dendrolimi have been widely used in biological control programs in recent years. However, its actual parasitic effect on the pest in the field is less, so it is important to develop attractants to increase the parasitic efficiency of the parasitoids. T . dendrolimi can be easily attracted by semiochemicals derived from flowers, intact plants, herbivore-attacked plants, and insects in its orchard habitats. In this paper, 73 semiochemicals were evaluated as potential attractants at three concentrations (0.1 mg/mL, 1 mg/mL, and 10 mg/mL) to T . dendrolimi by Y-tube olfactometer bioassays and Electroantennogram (EAG) technology, and then the mixtures of active individual compounds were further tested and evaluated in the field. As a result, 17 individual compounds showed attractive bioactivity to T . dendrolimi . Interestingly, when methyl salicylate and benzaldehyde mixed with linalool oxide or cis -3-hexenyl caproate in a volume ratio of 10:3:3 respectively showed excellent attractant bioactivity for T . dendrolimi in the orchard field. Thus, introducing such parasitoid attractants in orchard habitat may improve the biological control effect to pests.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 19
·11087
·
IF 6.208
Zhuangzhuang Li,Baoyan Ding,Mustafa R. K. Ali,Lizhen Zhao,Xiaoling Zang,Zhihua Lv
Abnormal tryptophan metabolism is linked to cancer and neurodegenerative diseases, and tryptophan metabolites have been reported as potential prostate cancer (PCa) biomarkers. However, little is known about the bioactivities of tryptophan metabolites on PCa cell growth. In this study, MTT and transwell assays were used to study the cytotoxicities of 13 major tryptophan metabolites on PCa and normal prostate epithelial cell lines. Ultraperformance liquid chromatography–high resolution mass spectrometry (UPLC–HRMS) was used to analyze metabolic changes in cells treated with tryptamine. Flow cytometry, confocal imaging, and Western blot were used to test the apoptosis induced by tryptamine. It was shown that tryptamine had obvious inhibitory effects on PCa cell lines PC-3 and LNCaP, stronger than those on the normal prostate cell line RWPE-1. Tryptamine was further shown to induce apoptosis and inhibit PC-3 cell migration. Metabolic changes including amino acid metabolism related to cell proliferation and metastasis were found in PC-3 cells treated with tryptamine. Furthermore, a PC-3 xenograft mouse model was used to study the effect of tryptamine in vivo. The intratumoral injection of tryptamine was demonstrated to significantly reduce the tumor growth and tumor sizes in vivo; however, intraperitoneal treatment resulted in increased tumor growth. Such dual effects in vivo advanced our understanding of the bioactivity of tryptamine in regulating prostate tumor development, in addition to its major role as a neuromodulator.Keywords:tryptamine;prostate cancer (PCa);cell proliferation;apoptosis;cell migration;xenograft model antitumor assays
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2022
·
IF 6.437
Bingbing Luo,Kai Wu,Jiajun Yu,Siyu Wang,Yihan Wang,Chenyang Chu,Huiyan Zhang
Catalytic pyrolysis of biomass for efficient N-heterocycles production is highly promising but extremely challenging. Herein, catalytic co-pyrolysis of glycerol and cellulose under ammonia atmosphere was proposed to enhance the N-heterocycles yield. First, a solvolysis effect of glycerol, which could improve the thermal depolymerization of cellulose, was observed in thermogravimetric analysis (TGA). After that, the major influential factors, including Si/Al ratio, reaction temperature, and glycerol-to-cellulose (Gly:Cel) ratio, were investigated systematically. The N-heterocycles yield, as well as the comprehensive synergies of feedstocks, reached their maximum value under the conditions of Si/Al = 85, 550 ℃, and Gly:Cel = 5:1. The best synergistic extents for pyrroles , pyridines, and indoles were 105.3%, 71.3%, and 38.8%, respectively. The mechanism insight showed that, the thermal decomposition of cellulose was promoted while the coking of oxygenated molecules was suppressed during the catalytic co-pyrolysis process. Consequently, more oxygenated molecules were generated and retained for further catalytic conversion into imine intermediates and N-heterocycles.
✓ 已验证
产品关联
DOI
高影响因子
Solar RRL
·
2021
·Vol. 6
·期 4
·2100995
·
IF 8.582
Chunyan Wang,Jihuai Wu,Shibo Wang,Zhongliang Yan,Xuping Liu,Guodong Li,Liqiang Chen,Sijia Zhu,Weihai Sun,Zhang Lan
Interface engineering has been proven to be an effective method to improve the performance and stability of perovskite solar cells (PSCs). Herein, 5-chloroindole (Cl-indole) is introduced into the interface of a SnO 2 electron transport layer and perovskite light-absorbing layer to improve the photovoltage performance of the device. The results show that Cl−indole can not only form hydrogen bonds with iodine in the perovskite to optimize the interface contact but also coordinate and passivate the Pb 2+ and Sn 4+ interface defects to alleviate carrier nonradiative recombination. Compared with the pristine SnO 2 -based planar PSCs with a power conversion efficiency (PCE) of 20.58%, the Cl−indole-modified SnO 2 -based device achieves a PCE of 22.47%. Meanwhile, the stability of the modified device is effectively improved and the hysteresis effect is reduced. This work demonstrates a promising strategy for high-efficient and stable PSCs by optimizing interface contact and passivating interface defects.
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Xudong Zhang,Junyao Wang,Jun Shen,Yugao Wang,Gang Liu,Yanxia Niu,Qingtao Sheng
Indole is an important organic synthesis intermediate derived from the wash oil fraction in coal tar. Deep eutectic solvents (DESs) have been widely used in the separation field due to their unique properties such as easy preparation, low cost, green and high extraction efficiency, and reusability. In this study, five DESs, including tetramethylammonium chloride/ethylene glycol (TMAC/EG), triethylmethylammonium chloride/ethylene glycol (TEMAC/EG), tetraethylammonium chloride/ ethylene glycol (TEAC/EG), tetrapropylammonium chloride/ethylene glycol (TPAC/EG), tetrabutylammonium chloride /ethylene glycol (TBAC/EG) were prepared and used to extract indole from model wash oil. The results showed that TPAC/EG exhibited the best extraction ability under the same conditions. Then, the main experimental parameters such as mass ratio of DES to model wash oil, extraction temperature, extraction time, standing time and initial concentration of indole, were investigated when using TPAC/EG as extractant. In optimal conditions (25℃, 5 min, mass ratio 1:8), the extraction efficiency, selectivity and distribution coefficient for indole were 90.51%, 79.21% and 61.12, respectively. Moreover, removing neutral oil with petroleum ether, the purity of indole further reached 96.63%, which was much higher than that in the previous studies reported. In addition, accessible and environmentally friendly water instead of diethyl ether was used to regenerate the DES. Reused four times, the TPAC/EG extraction ability for indole almost did not change. Finally, the mechanism of extraction process was investigated by FT-IR analysis. This work provides a new route for extraction and recovery indole from wash oil.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Cleaner Production
·
2021
·
IF 9.297
Antony Rajendran,Hong-Xia Fan,Tian-You Cui,Jie Feng,Wen-Ying Li
SO x emission, one of the significant contributors to air pollution, is primarily caused by the combustion of sulfur-containing liquid fuels . Thus, ultra-low sulfur or sulfur-free fuels production gains more attention to construct the SO x -free environment. Despite the oxidative desulfurization using polyoxometalates and hydrogen peroxide showing promising results, the requirement of hydrogen peroxide in excess and the complicated synthesis of heterogeneous polyoxometalates are the key drawbacks. So, in this paper, facile hydrothermal synthesis of mesoporous tin oxide-supported octamolybdates containing Mo V and Mo VI sites is reported for oxidative desulfurization with near stoichiometric hydrogen peroxide. The current catalytic system seems competitive among molybdenum-based oxidative desulfurization catalysts due to its higher turnover frequencies of 28.1 h −1 . The straightforward synthesis of catalysts and their excellent oxidative desulfurization activity would be attractive in building the SO x -free environment via clean transport energy.
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Microbiology
·
2021
·
IF 5.64
Zhao Shuxue,Chen Xi,Sun Qianshu,Wang Fei,Hu Chunhui,Guo Lizhong,Bai Jie,Yu Hao
Indole-3-acetic acid (IAA), known as a common plant hormone, is one of the most distributed indole derivatives in the environment, but the degradation mechanism and cellular response network to IAA degradation are still not very clear. The objective of this study was to elucidate the molecular mechanisms of IAA degradation at the protein level by a newly isolated strain Pseudomonas sp. LY1. Label-free quantitative proteomic analysis of strain LY1 cultivated with IAA or citrate/NH4Cl was applied. A total of 2,604 proteins were identified, and 227 proteins have differential abundances in the presence of IAA, including 97 highly abundant proteins and 130 less abundant proteins. Based on the proteomic analysis an IAA degrading (iad) gene cluster in strain LY1 containing IAA transformation genes (organized as iadHABICDEFG), genes of the β-ketoadipate pathway for catechol and protocatechuate degradation (catBCA and pcaABCDEF) were identified. The iadA, iadB, and iadE-disrupted mutants lost the ability to grow on IAA, which confirmed the role of the iad cluster in IAA degradation. Degradation intermediates were analyzed by HPLC, LC-MS, and GC-MS analysis. Proteomic analysis and identified products suggested that multiple degradation pathways existed in strain LY1. IAA was initially transformed to dioxindole-3-acetic acid, which was further transformed to isatin. Isatin was then transformed to isatinic acid or catechol. An in-depth data analysis suggested oxidative stress in strain LY1 during IAA degradation, and the abundance of a series of proteins was upregulated to respond to the stress, including reaction oxygen species (ROS) scavenging, protein repair, fatty acid synthesis, RNA protection, signal transduction, chemotaxis, and several membrane transporters. The findings firstly explained the adaptation mechanism of bacteria to IAA degradation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS Genetics
·
2021
·Vol. 17
·期 7
·e1009527
·
IF 5.917
Jun Yang,Hao Guo,Nan-Ji Jiang,Rui Tang,Guo-Cheng Li,Ling-Qiao Huang,Joop J. A. van Loon,Chen-Zhu Wang
Glucosinolates are token stimuli in host selection of many crucifer specialist insects, but the underlying molecular basis for host selection in these insects remains enigmatic. Using a combination of behavioral, electrophysiological, and molecular methods, we investigate glucosinolate receptors in the cabbage butterfly Pieris rapae . Sinigrin, as a potent feeding stimulant, elicited activity in larval maxillary lateral sensilla styloconica, as well as in adult medial tarsal sensilla. Two P . rapae gustatory receptor genes PrapGr28 and PrapGr15 were identified with high expression in female tarsi, and the subsequent functional analyses showed that Xenopus oocytes only expressing PrapGr28 had specific responses to sinigrin; when ectopically expressed in Drosophila sugar sensing neurons, PrapGr28 conferred sinigrin sensitivity to these neurons. RNA interference experiments further showed that knockdown of PrapGr28 reduced the sensitivity of adult medial tarsal sensilla to sinigrin. Taken together, we conclude that PrapGr28 is a gustatory receptor tuned to sinigrin in P . rapae , which paves the way for revealing the molecular basis of the relationships between crucifer plants and their specialist insects.
Preference of crucifer specialist insects to glucosinolates is well known in the field of insect-plant interactions, but its molecular basis is unclear. This study uses an integrative approach to investigate the molecular basis of glucosinolate detection by gustatory receptor neurons in the larval mouthparts and adult forelegs of the cabbage butterfly Pieris rapae , and finally reveal that PrapGr28 is a bitter receptor tuned to sinigrin. The current work takes a significant step towards identifying gustatory receptors tuned to glucosinolates, crucial recognition signals in crucifer host plants, providing insights into co-evolution of herbivorous insects and their host plants.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2021
·
IF 3.512
Huifang Yin,Hongping Chen,Meng Yan,Zhikun Li,Rongdi Yang,Yanjiao Li,Yanfang Wang,Jianyi Guan,Huili Mao,Yan Wang,Yuyang Zhang
Blue indigo dye, an important natural colorant, is used for textiles and food additives worldwide, while another red isomer, indirubin, is the major active ingredient of a traditional Chinese medicine named “Danggui Longhui Wan” for treating various diseases including granulocytic leukemia, cancer, and Alzheimer’s disease. In this work, we constructed a new and highly efficient indigoid production system by optimizing a novel terpenoid cyclase, XiaI, from the xiamycin biosynthetic pathway. Through introducing the flavin-reducing enzyme Fre, tryptophan-lysing and -importing enzymes TnaA and TnaB, and H2O2-degrading enzyme KatE and optimizing the fermentation parameters including temperature, the concentration of isopropyl-β-d-thiogalactopyranoside, and feeding of the l-tryptophan precursor, the final maximum productivity of indigoids by the recombinant strain Escherichia coli BL21(DE3) (XiaI-Fre-TnaAB-KatE) was apparently improved to 101.9 mg/L, an approximately 60-fold improvement to that of the starting strain E. coli BL21(DE3) (XiaI) (1.7 mg/L). In addition, when the fermentation system was enlarged to 1 L in the flask (feeding with 5 mM tryptophan and 10 mM 2-hydroxyindole), the indigoid productivity further increased to 276.7 mg/L at 48 h, including an indigo productivity of 26.0 mg/L and an indirubin productivity of 250.7 mg/L, which has been the highest productivity of indirubin so far. This work provided a basis for the commercial production of bio-indigo and the clinical drug indirubin in the future.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）