ADVANCED MATERIALS
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2024
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IF 27.4
Wen Li,Qi-Wen Chen,Jin-Xuan Fan,Zi-Yi Han,Wen-Fang Song,Xuan Zeng,Xian-Zheng Zhang
Due to inherent differences in cellular composition and metabolic behavior with host cells, tumor-harbored bacteria can discriminatorily affect tumor immune landscape. However, the mechanisms by which intracellular bacteria affect antigen presentation process between tumor cells and antigen-presenting cells (APCs) are largely unknown. The invasion behavior of attenuated Salmonella VNP20009 (VNP) into tumor cells is investigated and an attempt is made to modulate this behavior by modifying positively charged polymers on the surface of VNP. It is found that non-toxic chitosan oligosaccharide (COS) modified VNP (VNP@COS) bolsters the formation of gap junction between tumor cells and APCs by enhancing the ability of VNP to infect tumor cells. On this basis, a bacterial biohybrid is designed to promote in situ antigen cross-presentation through intracellular bacteria induced gap junction. This bacterial biohybrid also enhances the expression of major histocompatibility complex class I molecules on the surface of tumor cells through the incorporation of Mdivi-1 coupled with VNP@COS. This strategic integration serves to heighten the immunogenic exposure of tumor antigens; while, preserving the cytotoxic potency of T cells. A strategy is proposed to precisely controlling the function and local effects of microorganisms within tumors.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Zhimin Deng,Hua Liu,Gantao Chen,Hongbing Deng,Xiangyang Dong,Linlin Wang,Fenghua Tao,Fangfang Dai,Yanxiang Cheng
Ovarian cancer , the deadliest gynecological malignancy, primarily treated with chemotherapy. However, systemic chemotherapy often leads to severe toxic side effects and chemoresistance. Drug-loaded aerogels have emerged as a promising method for drug delivery, as they can improve drug solubility and bioavailability, control drug release, and reduce drug distribution in non-targeted tissues, thereby minimizing side effects. In this research, chitosan oligosaccharide (COS)-loaded nanofibers composite chitosan (CS) aerogels (COS-NFs/CS) with a porous network structure were created using nanofiber recombination and freeze-drying techniques. The core layer of the aerogel has a COS loading rate of 60 %, enabling the COS-NFs/CS aerogel to significantly inhibit the migration and proliferation of ovarian cancer cells (resulting in a decrease in the survival rate of ovarian cancer cells to 33.70 % after 48 h). The coaxial fiber's unique shell-core structure and the aerogel's porous network structure enable the COS-NFs/CS aerogels to release COS steadily and slowly over 30 days, effectively reducing the initial burst release of COS. Additionally, the COS-NFs/CS aerogels exhibit good biocompatibility , degradability (only retaining 18.52 % of their weight after 6 weeks of implantation), and promote angiogenesis, thus promoting wound healing post-oophorectomy. In conclusion, COS-NFs/CS aerogels show great potential for application in the treatment of ovarian cancer.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Nanqi Zhao,Chaofan Guo,Ziyao Liu,Lin Chen,Yayun Hu,Minyi Han,Feng Huang,Zhuangli Kang,Xianchao Feng
This study investigated the effect of different hydrocolloids on the improvement of the printability and post-processing stability of minced chicken meat, each hydrocolloid was prepared with 1 % formulation and compared with the control. The effects of these hydrocolloids on the rheological properties of chicken mince and complex model printing capability were explored separately, while the cooking loss and microstructure changes of the samples before and after heating were analyzed. The results showed that the chicken mince gel containing carrageenan was more suitable for printing, increased the yield stress and apparent viscosity of the samples, and the printing process was easier to mold. In addition, carrageenan increased the hardness of the samples, and the microstructures were compact and changed little during the heating process, and the water was locked in the gel matrix, reducing shape changes during the heating process. The use of hydrocolloids improves the stability of post-processing of chicken 3D printing.
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Foods
·
2024
·Vol. 13
·期 7
·1109
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IF 4.7
Zilin Wang,Ye Zhao,Min Yang,Yuanli Wang,Yue Wang,Chongying Shi,Tianyi Dai,Yifan Wang,Liang Tao,Yang Tian
Finding stable and bioavailable calcium supplements is crucial for addressing calcium deficiency. In this study, glycated peptide–calcium chelates (WMPHs–COS–Ca) were prepared from walnut meal protein hydrolysates (WMPHs) and chitosan oligosaccharides (COSs) through the Maillard reaction, and the structural properties and stability of the WMPHs–COS–Ca were characterized. The results showed that WMPHs and COSs exhibited high binding affinities, with a glycation degree of 64.82%. After glycation, Asp, Lys, and Arg decreased by 2.07%, 0.46%, and 1.06%, respectively, which indicated that these three amino acids are involved in the Maillard reaction. In addition, compared with the WMPHs, the emulsifying ability and emulsion stability of the WMPHs–COS increased by 10.16 mg2/g and 52.73 min, respectively, suggesting that WMPHs–COS have better processing characteristics. After chelation with calcium ions, the calcium chelation rate of peptides with molecular weights less than 1 kDa was the highest (64.88%), and the optimized preparation conditions were 5:1w/wfor WMPH–COS/CaCl2s, with a temperature of 50 °C, a chelation time of 50 min, and a pH of 7.0. Scanning electron microscopy showed that the “bridging role” of WMPHs-COS changed to a loose structure. UV–vis spectroscopy and Fourier transform infrared spectrometry results indicated that the amino nitrogen atoms, carboxyl oxygen atoms, and carbon oxygen atoms in WMPHs-COS chelated with calcium ions, forming WMPHs-COS-Ca. Moreover, WMPHs-COS-Ca was relatively stable at high temperatures and under acidic and alkaline environmental and digestion conditions in the gastrointestinal tract, indicating that WMPHs–COS–Ca have a greater degree of bioavailability.
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Food Bioscience
·
2024
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IF 4.8
Kangshuai Lou,Yuanrong Zheng,Hongmei Yu,Xiaohan Tan,Libin Wang,Changmin Tong,Siqiang Huang,Xintong Cai,Changyu Zhou,Jinxuan Cao,Daodong Pan,Zhen Wu,Hao Zhang,Qiang Xia
This study investigated the feasibility of protein/chitosan-based Pickering emulsions as a fat replacer in low-fat cheddar cheese. It compared the chemical composition, functional properties, and microstructure of full-fat cheese (FFC) with reduced-fat cheese (RFC) and low-fat cheese (LFC), both utilizing a whey protein isolate-chitosan oligosaccharide (WPI-COS) microgel-stabilized emulsion as a fat substitute. The results indicated that cheese yield decreased following the substitution with fat replacers, although the cheese had higher protein and moisture contents, and lower fat content. Color analysis revealed that WPI-COS microgels significantly mitigated color defects in low-fat cheese. The use of WPI-COS microgels as matrix fillers resulted in a denser cheese structure, but simultaneously observing cheese collapse associated with poor meltability and denser structure. This observation corresponded to increased hardness, elasticity, and G′, thus demonstrating improved chewiness. Scanning electron microscopy observations demonstrated that the microstructures of RFC and LFC were more uniform and smoother. Confocal laser scanning microscopy revealed smaller and more uniform fat globules in RFC and LFC groups, preventing quality issues caused by fat aggregation. Compared to FFC, RFC and LFC groups had higher levels of fatty acid, especially caproic, nonanoic, and capric acids, providing a rich fatty flavor. Hydrocarbons, aromatic, and heterocyclic compounds also contributed to cheese flavor, as well as the fruity taste produced by toluene. Additionally, nonanal compounds were detected due to the action of WPI-COS which imparted a greasy and citrus flavor to the low-fat cheese. These findings suggested that WPI-COS microgel-stabilized Pickering emulsions could be a feasible way as fat substitutes of cheddar cheese, offering potential benefits for reducing fat bioaccessibility in dairy products. This approach may have significant implications for the dairy industry, particularly in the development of healthier low-fat cheese options, as well as for public health by offering a viable strategy to reduce saturated fat consumption.
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INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
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2024
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IF 2.5
Shujie Cheng,Shimin Wu,Jianqiang Lan
Polycyclic aromatic hydrocarbons (PAHs) generated from heating butter pose a potential threat to consumer health. This study involved heating butter under different conditions and analysing the changes in the content of 54 PAHs. Furthermore, we investigated the inhibitory effects of β-carotene, pure astaxanthin and astaxanthin microcapsule powder on PAH formation. Results showed that heating temperature and duration affect the formation and transformation of PAHs, with higher temperatures facilitating the synthesis of oxygenated PAHs, whereas longer heating times promote the accumulation of light PAHs. Despite an observed increase in the concentration of light PAHs following heat treatment with added antioxidants, the antioxidants significantly reduced the toxic equivalency quotients (TEQs) in butter. This study revealed the PAH production pattern during butter heating and the effectiveness of antioxidants in inhibiting their accumulation.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiaokang Na,Bowen Zou,Xiaohan Zheng,Xueer Yu,Ling Zhang,Xianbing Xu,Ming Du,Chao Wu
The persistent global issues of unsafe food and food waste continue to exist. Microbial contamination stands out as a major cause of losses in perishable foods like vegetables and fruits. Herein, we report a self-assembling coating based on disulfide bond cleavage-induced bovine serum albumin (BSA), where the antimicrobial activity of chitosan oligosaccharide (COS) is stably anchored in the coating by electrostatic interactions during the unfolding-aggregation phase of BSA. The intrinsic antimicrobial activity of COS, combined with the positively charged and hydrophobic regions enriched on the BSA coating, significantly disrupts the integrity of bacterial structures. Furthermore, the BSA@COS coating can easily adhere in situ to the grooves on the surface of strawberries through a simple one-step spraying process, extending the shelf life of strawberries and bananas by nearly three times. This makes it a potential economic alternative to current commercial antimicrobial coatings, offering a solution to the rampant global issue of food waste.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jia Wan,Meifang Yin,Feng Wang,Guangtao Huang,Ilaria Dal Pra,Yongzhi Liang,Jun Wu
Bacterial infections following skin injuries can lead to chronic, hard-healing wounds, and the overuse of antibiotics has made infection control a significant clinical challenge. Nature provides numerous antimicrobial agents, inspiring the design of novel antibacterial wound dressings; however, their solubility often presents additional hurdles. Herein, we leverage highly water-soluble chitosan oligosaccharide molecules dispersed within a gelatin-based gel network to overcome the poor solubility of chitosan, a known antibacterial agent. Notably, we first discovered that chitosan oligosaccharides can promote the solubility of poorly water-soluble antibacterial molecules like emodin through Schiff base reactions, thereby enabling synergistic antibacterial effects. The resulting eutectogel exhibits excellent antibacterial activity against both Staphylococcus aureus and Escherichia coli. Additionally, the gel demonstrates superior tensile strength, fracture strain, resistance to desiccation, and biocompatibility, with its outstanding overall performance laying a foundation for wound dressing applications. In animal models of infected wounds, the gel effectively accelerates wound healing, achieving wound closure rates of 166.9 % and 136.9 % of the control group on days 4 and 7, respectively. Mechanistic studies further reveal that the dressing promotes cellular proliferation, migration, and the regeneration of blood vessels and collagen, contributing to the wound healing process.
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CARBOHYDRATE RESEARCH
·
2025
·
IF 2.5
Yuting Sun,Junnan He,Yongyan Sun,Lidong Wang,Haidu Li,Yaqi Miao,Ping Shi,Jin Zhang,Kaixuan Luo,Yan Zhao
The development of reactive oxygen species (ROS)-responsive nanodrug delivery systems faces critical challenges. Excessive cross-linking degree prevents complete degradation of nanocarriers under tumor microenvironment ROS levels, significantly compromising drug release efficiency. Conversely, insufficient cross-linking degree impairs the structural stability of nanoparticles, resulting in premature drug leakage during systemic administration. Herein, we present an innovative strategy for constructing dual cross-linked chitooligosaccharide nanoparticles (NPs) co-loaded with curcumin and glucose oxidase (GOx) to enhance structural stability. These engineered NPs can amplify intracellular ROS concentration through GOx-mediated catalytic conversion of glucose to H 2 O 2 , thereby establishing a ROS-triggered self-accelerating drug release nanosystem for enhanced tumor chemotherapy. In vitro studies have shown that the curcumin release behavior of CTCG NPs depends on the GOx encapsulated inside the nanoparticles. Compared with nanoparticles without GOx, CTCG NPs can effectively prevent the premature release of curcumin in a simulated normal physiological environment. The nanoparticles also showed significant anti-tumor effect and could effectively inhibit the proliferation of tumor cells in vivo . These results proved that CTCG NPs achieve controllable release of drugs through in situ production of ROS in the tumor microenvironment. This nanodrug delivery system shows promising potential for breast cancer therapy.
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ACS Applied Bio Materials
·
2024
·
IF 4.6
Zhaoxin Xia,Yulong Liao,Ge Gao,Shiyong Zhang
The poor accumulation of antibiotics in the cytoplasm leads to the poor eradication of intracellular bacteria. Herein, a polyelectrolyte complex (PECs@Rif) allowing direct cytosolic delivery of rifampicin (Rif) was developed for the treatment of intracellular infections by complexation of poly(α-lipoic acid) (pLA) and oligosaccharide (COS) in water and loading Rif. Due to the thiol-mediated cellular uptake, PECs@Rif delivered 3.9 times higher Rif into the cytoplasm than that of the free Rif during 8 h of incubation. After entering cells, PECs@Rif released Rif by dissociating pLA into dihydrolipoic acid (DHLA) in the presence of intracellular thioredoxin reductase (TrxR). Notably, DHLA could reduce endogenous Fe(III) to Fe(II) and provide a catalyst for the Fenton reaction to produce a large amount of reactive oxygen species (ROS), which would assist Rif in eradicating intracellular bacteria. In vitro assay showed that PECs@Rif reduced almost 2.8 orders of magnitude of intracellular bacteria, much higher than 0.7 orders of magnitude of free Rif. The bacteremia-bearing mouse models showed that PECs@Rif reduced bacterial levels in the liver, spleen, and kidney by 2.2, 3.7, and 2.3 orders of magnitude, respectively, much higher than free Rif in corresponding tissues. The direct cytosolic delivery in a thiol-mediated manner and enhanced oxidative stress proposed a feasible strategy for treating intracellular bacteria infection.
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FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Hongmei Yu,Yuanrong Zheng,Kangshuai Lou,Changyu Zhou,Jinxuan Cao,Lihui Du,Yangying Sun,Jun He,Daodong Pan,Zhendong Cai,Qiang Xia
To elucidate the roles of interfacial protein microstructure in regulating techno-functional attributes of complex microgel, this work explored the effects of transglutaminase cross-linking alone or in combination with ultrasonication on conformation and functional properties of whey protein isolate-chitooligosaccharide microgel dispersion (WPI–COS). It was found that transglutaminase-crosslinked WPI-COS microgels presented improved interfacial properties when a moderate unfolding process was induced by sonication, validating the existence of optimum flexibility of complex Pickering particles during unfolding/refolding of interfacial proteins. Particularly, TG induced excessive crosslinking of WPI-COS to form aggregates, causing a lower surface hydrophobicity. In contrast, ultrasound was inclined to increase the surface hydrophobicity for TG-catalyzed and uncatalyzed samples, suggesting differential unfolding degrees of WPI induced by transglutaminase and ultrasonication. Correspondingly, interfacial properties of ultrasonicated WPI-COS complexes, as revealed by percentage of adsorbed protein, interfacial tension and three-phase contact angle and emulsifying properties, were improved significantly. Confocal laser scanning microscopy and transmission electron microscopy consistently observed WPI-COS tightly adsorbed at the oil-water interface, with smaller emulsion droplets, more uniform distribution and thicker interfacial films at the oil-water interface in WPI-COS microgel particles with sonication-induced unfolding. This investigation demonstrated the feasibility of conformational regulation of interfacial proteins in tuning techno-functional features of microgel particles.
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CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Wei Wan,Rui Zhang,Lingyi Gao,Aijun Jian,Xianbing Xu,Xiaokang Na,Ming Du,Jianyu Zhu,Beiwei Zhu,Chao Wu
The strategic utilization of natural polysaccharides presents a critical approach for enhancing the gelation properties of soy protein isolate (SPI), which is a key factor in developing superior plant-based food textures. This study demonstrates a strategic technique to enhance SPI gel properties via preheating treatment and electrostatic complexation with chitosan oligosaccharide (COS). Structural analyses revealed that preheating and electrostatic complexation induced SPI unfolding, facilitating conformational transitions toward β-sheet (increased from 53.00 % to 65.35 %) and β-turn (increased from 5.10 % to 7.60 %) configurations while exposing buried tryptophan residues. Fourier-transform infrared spectroscopy, surface hydrophobicity, measurement of interaction force, and molecular docking confirmed that SPI-COS complexation was driven by synergistic electrostatic attractions, hydrogen bonds, van der Waals forces, and hydrophobic interactions. Increasing COS concentration (0–0.16 %, w / v ) progressively enlarged complex particle size (100–350 nm) but reduced colloidal stability, as evidenced by turbidity, ζ-potential, and cryo-scanning electron microscopy. Critically, COS incorporation at 0.08–0.16 % ( w / v ) significantly elevated storage modulus (increased by 275 %) and viscosity while optimizing the regulation of bound and immobilized water in a concentration-dependent manner. These findings establish that COS-mediated electrostatic complexation restructures SPI networks into mechanically robust gels, providing a green strategy for next-generation plant-based foods.
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危险品化学品经营许可证（带存储）