MICROCHIMICA ACTA
·
2022
·Vol. 190
·期 1
·1-11
·
IF 6.408
Xu Yixuan,Shi Xuerong,Ran Fanpeng,Zhang Ziqi,Phipps Josh,Liu Xiaoyan,Zhang Haixia
A novel Tb-doped Eu-based metal–organic framework (Eu-MOF@Tb) has been developed by incorporating hexanuclear europium cluster and 2,2′-bipyridine-5,5′-dicarboxylic acid as well as coordination with Tb(III). Owing to the diverse coordination status of Tb(III) and Eu(III) in MOF, antenna effect emission from Tb(III) can be invoked by dipicolinic acid (DPA), but the luminescence originating from Eu(III) remains unchanged. Taking advantage of this phenomenon, a ratiometric luminescent method for detection of DPA, a biomarker for Bacillus subtilis spores, was developed through differential sensitization toward lanthanide ions. This analysis method allowed for the detection of DPA in the 0.2–10 μM concentration range, with a detection limit of 60 nM. It was further validated by spiked recoveries (89.3–110%) of real-world samples with RSD values in the range 3.9–11%. Alongside this, a paper indicator test was prepared for naked-eye detection of DPA via a dose-sensitive color evolution from red to green under UV light. The effectiveness of the proposed approach was explored in the detection of bacterial spores in real biological and environmental samples and indicated great potential for applications as a real-time monitoring system against the anthrax threat. Graphical Abstract
研究领域:
分析化学
·
生物化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·Vol. 104
·期 11
·6657-6666
·
IF 3.3
Yaoyao Qi,Shun Zhong,Feng Pan,Junfu Zhou,Zhiyu Wang,Zeyuan Deng,Hongyan Li
BACKGROUND The hard double-walled structure of Ganoderma lucidum spore powder (GLSP) is difficult for the human body to digest, so it is very important to break the wall of GLSP. In this study, the wall of GLSP was broken by mechanical milling at room temperature (MM-R) and ultra-fine grinding at low temperature (UFG-L), respectively. RESULTS Compared with MM-R, UFG-L could better retain the sporangium powder's morphological and structural integrity. During in vitro digestion, compared with unbroken GLSP, the released amounts of polysaccharides and triterpenes from broken GLSP were significantly increased, and they increased with the increase of specific surface area. The bioaccessibility of polysaccharide and triterpene from unbroken GLSP after the intestinal stage were 29.52% and 5.37%, respectively. The bioaccessibility of polysaccharides and triterpene from broken GLSP by MM-R after the intestinal phase were 39.73–72.45% and 16.44–24.97%, while those by UFG-L were 44.53–104.18% and 12.96–32.90%, respectively. CONCLUSION The active ingredients of broken GLSP showed better digestion and absorption abilities than unbroken GLSP. Moreover, the specific surface area of GLSP by UFG-L was lower than that by MM-R, and the bioaccessibility of GLSP by UFG-L was higher than that by MM-R. © 2024 Society of Chemical Industry.
研究领域:
食品科学
·
营养学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
STAR Protocols
·
2024
·
IF 1.3
Miner Deng,Yanting Wei,Na Li,Lihua Xiao,Yaoyu Feng,Yaqiong Guo
Summary A major bottleneck in the progress of Cryptosporidium research is the lack of accessible cryopreservation of Cryptosporidium oocysts. Here, we present a protocol for the cryopreservation of Cryptosporidium isolates using enteroids. We describe the steps for the establishment of enteroid cultures and cryopreservation of C. parvum -infected HCT-8 cultures. We then detail procedures for the recovery and propagation of frozen parasites using enteroids. For complete details on the use and execution of this protocol, please refer to Deng et al. 1
研究领域:
微生物学
·
寄生虫学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Parasites & Vectors
·
2024
·Vol. 17
·期 1
·1-10
·
IF 3
Huang Yue,Li Jinli,Pei Shifeng,You Heng,Liu Huimin,Guo Yaqiong,Xu Rui,Li Na,Feng Yaoyu,Xiao Lihua
Background The dimerizable Cre recombinase system (DiCre) exhibits increased leaky activity in Cryptosporidium, leading to unintended gene editing in the absence of induction. Therefore, optimization of the current DiCre technique is necessary for functional studies of essential Cryptosporidium genes.Methods Based on the results of transcriptomic analysis of Cryptosporidium parvum stages, seven promoters with different transcriptional capabilities were screened to drive the expression of Cre fragments (FKBP-Cre59 and FRB-Cre60). Transient transfection was performed to assess the effect of promoter strength on leakage activity. In vitro and in vivo experiments were performed to evaluate the leaky activity and cleavage efficiency of the optimized DiCre system by polymerase chain reaction (PCR), nanoluciferase, and fluorescence analyses.Results The use of promoters with lower transcriptional activity, such as pcgd6_4110 and pcgd3_260, as opposed to strong promoters such as pActin, pα-Tubulin, and pEnolase, reduced the leakage rate of the system from 35–75% to nearly undetectable levels, as verified by transient transfection. Subsequent in vitro and in vivo experiments using stable lines further demonstrated that the optimized DiCre system had no detectable leaky activity. The system achieved 71% cleavage efficiency in vitro. In mice, a single dose of the inducer resulted in a 10% conditional gene knockout and fluorescent protein expression in oocysts. These fluorescently tagged transgenic oocysts could be enriched by flow sorting for further infection studies.Conclusion sA DiCre conditional gene knockout system for Cryptosporidium with good cleavage efficiency and reduced leaky activity has been successfully established.Graphical Abstract
研究领域:
寄生虫学
·
分子生物学
·
基因组编辑
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2018
·
IF 5.711
Fenglei Chen,Fang Tang,Chih-Tsung Yang,Xinyao Zhao,Jun Wang,Benjamin Thierry,Vipul Bansal,Jianjun Dai,Xin Zhou
Cryptosporidium parvum (C. parvum) is a highly potent zoonotic pathogen, which can do significant harm to both human beings and livestock. However, existing technologies or methods are deficient for rapid on-site detection of water contaminated with C. parvum. Better detection approaches are needed to allow water management agencies to stop major breakouts of the pathogen. Herein, we present a novel detection method for cryptosporidium in a tiny drop of sample using a magnetic nanoparticle (MNP) probe combined with dark-field microscopy in 30 min. The designed MNP probes bind with high affinity to C. parvum, resulting in the formation of a golden garland-like structure under dark-field microscopy. This MNP-based dark-field counting strategy yields an amazing PCR-like sensitivity of 8 attomolar (aM) (5 pathogens in 1 μL). Importantly, the assay is very rapid (∼30 min) and is very simple to perform as it involves only one step of mixing and magnetic separation, followed by dropping on a slide for counting under dark-field microscope. By combining the advantages of the specific light-scattering characteristic of MNP probe under dark field and the selective magnetic separation ability of functionalized MNP, the proposed MNP-based dark-field enumeration method offers low cost and significant translational potential.
研究领域:
寄生虫学
·
环境科学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE
·
2025
·
IF 3.9
Li-Peng Shan,Mao-Mao Wang,Yang Hu,Lei Liu,Bin Zhu,Jiong Chen
Hepatopancreatic microsporidiosis (HPM), caused by Enterocytozoon hepatopenaei (EHP), has emerged as a major threat to the shrimp aquaculture industry. However, no effective strategies have yet been developed to control the spread of this disease. In this study, we assessed the anti-EHP activity of ten monomeric compounds in Penaeus vannamei . The results showed that tetrahydropalmatine reduced the EHP copy number by 73.8 % at a concentration of 20 mg/L. In a therapeutic experiment, feeding EHP-infected shrimp a diet containing 0.1 % tetrahydropalmatine significantly lowered the EHP load. Additionally, the growth-related gene juvenile hormone esterase-like carboxylesterase 1 was significantly upregulated, while ecdysteroid-regulated protein and farnesoic acid O-methyltransferase were significantly downregulated, suggesting that tetrahydropalmatine may enhance the growth potential of EHP-infected shrimp. After 30 days of feeding, the EHP-infected shrimp's body weight in the groups fed with tetrahydropalmatine increased by 19.9 % compared to those fed with the normal diet. In a prevention experiment, healthy shrimp fed a diet containing tetrahydropalmatine and subsequently infected with EHP showed an increase in body weight by day 30 post-infection, compared to the control group, although this difference was not statistically significant. Tetrahydropalmatine can inhibit spore germination and electron dense disc formation, which may be an important reason for its suppression of EHP infection. Moreover, tetrahydropalmatine effectively inhibited the horizontal transmission of EHP, suggesting its potential to limit HPM outbreaks. These findings indicate that tetrahydropalmatine may serve as a feed additive for the treatment of HPM caused by EHP.
研究领域:
水产养殖学
·
寄生虫学
·
分子生物学
✓ 已验证
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Mengge Shang,Jing Zhang,Xiaochan Liu,Yi Liu,Sipeng Guo,Shimo Yu,Serguei Filatov,Xibin Yi
A novel N, S self-doped hollow-sphere porous carbon is directly prepared from abundant biomass puffball spores via a simple carbonization and KOH activation process for the first time. The electrochemical properties and porous structure of these carbons are explored by adjusting the amount of KOH. It is found that the obtained N, S self-doped hollow-sphere porous carbon with a KOH to carbon mass ratio of 4 (NS-HPC-4) exhibits the largest specific surface area (1431 m 2 /g), a well-developed hierarchical porous structure and moderate N/S contents. As a result, the NS-HPC-4 electrode in the supercapacitor delivers a large specific capacitance of 285 F/g at 0.5 A/g in KOH electrolyte. Furthermore, the symmetric supercapacitor based on NS-HPC-4 exhibits an energy density of 10.9 Wh/kg at a power density of 400.4 W/kg in 2 M KOH aqueous electrolyte, and achieves a high energy density of 58.4 Wh/kg at a high power density of 876.0 W/kg in 1 M Et 4 NBF 4 /AN organic electrolyte. These excellent results confirm that puffball spores derived porous carbons are promising candidates to be used in high performance supercapacitors.
研究领域:
材料科学
·
能源存储
·
生物质利用
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Delong Wang,Haibin Ni,Zhongqiang Wang,Bing Liu,Hongyuan Chen,Zhongze Gu,Xiangwei Zhao
Bacteria identification plays a vital role in the field of clinical diagnosis, food industry, and environmental monitoring, which is in great demand of point of care detection methods. In this paper, in order to discriminate the source of nosiheptide product, a plasmonic filter was fabricated to filtrate, capture and identify Streptomycete spores with Surface enhanced Raman Scattering (SERS). Since the plasmonic filter was derived from self-assembled photonic crystal coated with silver, the plasmonic “hot spots” on the filter surface was distributed evenly in a fare good density and the SERS enhancement factor was 7.49 × 107. With this filter, a stain- and PCR-free detection was realized with only 5 μL sample solution and 5 min in a manner of “filtration and measure”. Comparison to traditional Gram stain method and silver-plated nylon filter membrane, the plasmonic filter showed good sensitivity and efficiency in the discrimination of nosiheptide prepared with chemical and biological methods. It is anticipated that this simple SERS detection method with plasmonic filter has promising potentials in food safety, environmental, or clinical applications.
研究领域:
微生物学
·
临床诊断
·
食品安全
·
环境监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION B-A JOURNAL OF CHEMICAL SCIENCES
·
2021
·
IF 1.047
Chun Yang,Lili Song,Zhong Miao,Lingyun Jiang,Ting Li,Xiaoyan Zhi,Xiaojuan Hao,Hui Cao
To explore candidate fungicides from plant secondary metabolites, 16 novel obovatol-type phenazine derivatives were semi-synthesized from obovatol isolated from the leaves of Magnolia obovata Thunb. The antifungal activity of synthesized compounds was investigated in vitro against four phytopathogenic fungi using the spore germination method. The bioassay results showed that eight derivatives ( 8b , 8g , 8h – k , 8i′ , and 8k′ ) exhibited better antifungal activity against Fusarium solani than two positive controls, especially compounds 8b (IC 50 = 64.61 μg mL −1 ) and 8i′ (IC 50 = 79.97 μg mL −1 ) showed pronounced inhibition of spore germination activity against F. solani . They could be used as lead compounds for further structural optimization. Additionally, the preliminary structure-activity relationships (SARs) illustrated that the introduction of a benzene ring monosubstituted with electron-withdrawing groups into the obovatol scaffold could lead to potentially antifungal compounds.
研究领域:
植物化学
·
药物化学
·
真菌学
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Ruixing Chen,Ang Ma,Jingjing Liao,Yuqing Meng,Junzhe Zhang,Herong Li,Hailu Yao,Zipeng Gong,Hai-Ning Lyu
Bioactive proteins and peptides hold significant potential in the food industry, particularly for the development of functional foods and nutraceuticals. However, their effectiveness is often limited by poor oral bioavailability. While various protein delivery systems have been explored, challenges such as safety concerns and low protein encapsulation efficiency continue to hinder their use in food applications. This study presents a novel approach to enhancing protein delivery by chemically modifying Ganoderma lucidum spores (GLS) with charged surfaces to improve their protein-binding capabilities. Acid and alkali treatments were employed to modify the surface charge of GLS, while maintaining their structural integrity. The resulting GLS microcarriers demonstrated significantly enhanced protein-loading and encapsulation, achieving up to 34.93% (349.34 mg/g) loading capacity and 69.87% encapsulation efficiency using vacuum loading. The protein release kinetics under simulated gastric and intestinal conditions were investigated, and the role of electrostatic adsorption in this protein delivery system, which contributes to the enhanced protein-loading performance, was also discussed. These findings highlight GLS microcarriers as a promising, food-grade platform for high-capacity protein delivery, offering a practical and safe solution to current challenges in the incorporation of functional proteins into food products.
研究领域:
食品科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 60
·34772-34783
·
IF 3.049
Yanzhuo Zhu,Honghui Teng,Dongshu Sun,Dayu Jiang,Yongsheng Yan
In this work, a new and simple method was developed for the synthesis of uncovered and high-selectivity spiramycin-based molecularly imprinted microspheres (SP-MIMs) by Pickering emulsion polymerization using spiramycin as templates. And surprisingly the solid particles were absent on the surface of imprinted microspheres, which can be ascribed to the Ganoderma lucidum spores (GLS): they were firstly selected to be the stabilizers for the Pickering emulsion in this new strategy. Through a series of adsorption experiments, the uncovered SP-MIMs were proven to possess more excellent selective recognition and removal ability for template SP in aqueous solution. And SP-MIMs could be reused for many times without significant loss of adsorption capacity, indicating the satisfactory regeneration performance. Therefore, SP-MIMs can be employed as a promising adsorbent for the selective removal of SP from aqueous media, and this strategy will contribute to overcoming the shortcomings of stabilizer particles on the surface of the as-prepared imprinting microspheres, because these solid particles lack the special selective recognition activity of template molecules, and may disturb the adsorption effect of imprinting microspheres.
研究领域:
材料科学
·
药物化学
✓ 已验证
DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2020
·
IF 4.303
Jinxin Che,Yue Chen,Yalan Wu,Lu Li,Nengguo Tao
Tremendous economic losses in the citrus industry are caused by infestations of Penicillium digitatum . Previous research has shown that the terpene myrcene in citrus essential oils could stimulate the spore germination and mycelial growth of P. digitatum . However, the underlying mechanism is poorly known. Here, how myrcene induces the spore germination of P. digitatum was elucidated. The addition of myrcene significantly promoted the spore germination of P. digitatum and green mold in citrus fruit. As the incubation time increased, the intracellular and extracellular myrcene contents both significantly decreased in myrcene-treated samples, and no downstream products were detected through a Gas Chromatography-Mass Spectrometer. Metabolomics analysis revealed that the contents of metabolites involved in central carbon metabolism, such as glycolysis, pentose phosphate pathway, and citrate cycle, were significantly affected after the addition of myrcene. The adenosine triphosphate content and the intracellular energy charge were also drastically increased. Overall, our results suggest that the ability of myrcene to stimulate the spore germination of P. digitatum might be mediated by the upregulation of central carbon and energy metabolism.
研究领域:
微生物学
·
植物科学
·
代谢组学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）