INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
·
IF 6.953
Mengtian Yu,Dongpo He,Yuhang Zhang,Dahao He,Xing Wang,Jinghui Zhou
Acanthopanax senticosus has been used to extract active products. However, abundant Acanthopanax senticosus residues (ASR), which contain plenty of lignin are discarded after extraction. An appropriate extraction method should be chosen to obtain the lignin with such desirable properties. Thus, this study investigated the effect of alkali, milled wood, deep eutectic solvent and ethanol methods on the lignin. Lignin obtained from different extraction methods were characterized, yields, chemical structure, thermal behavior, molecular weight and phenolic content were evaluated. The results show that the process of lignin acquisition has a great influence on the properties of lignin. Moreover, the multifarious functional groups exist in lignin macromolecules , such as phenolic, ether groups and other chromophores, conferred good UV resistance to lignin. Among them, the lignin from alkali method has the most phenolic-OH groups and smallest molecular weight result in a good UV-resistant, the SPF value achieves 2.39 at 1% AL content, the alkali method was the best way to make sunscreen blended with cream take various factors into consideration. This study used lignin as a bioactive ingredient to provide UV-resistant property to sunscreen formulations. Furthermore, lignin extracted from Acanthopanax senticosus residue provides a new application for the treatment of herb residue waste.
研究领域:
植物化学
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材料科学
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药用植物研究
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ying Wang,Xinhui Bi,Xuhui Huang,Dongmei Li,Lei Qin,Yuying Zhang
Due to the complexity of animal matrices and the different polarities of lipids, the measurement methods are expected to cover a comprehensive range of lipid species. Three extraction methods (chloroform/methanol, methyl tert -butyl ether, and Soxhlet methods) and three resuspension solvents (methanol, isopropanol/acetonitrile = 1:1, and isopropanol/acetonitrile = 9:1) were used to compare the lipid species, contents, and ionization efficiency of Spanish mackerel and duck muscles. Overall, the chloroform/methanol extraction method was the most effective in capturing a diverse array of lipid species. The redissolution solvent isopropanol/acetonitrile (1,1) was highly efficient for detecting triglycerides, while methanol was effective in detecting diacylglycerols and phospholipids. Optimizing the measurement conditions of lipids with different polarities provides a valuable reference for lipid extraction and structural elucidation in different muscle tissues. Furthermore, it supports the determination of differential markers for animal lipid metabolism studies or food authentication using an untargeted lipidomic approach.
研究领域:
分析化学
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生物化学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
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IF 8.1
Xiaoqing Huang,Haoyu Su,Ping Hu,Yueyuan Ye,Duo Wang
To fully utilized the high value-added phenolic compounds in biomass pyrolysis bio-oils, a heterogeneous extraction method was developed in this study to separate 4-ethylguaiacol, guaiacol, and eugenol from bio-oil one by one. Based on the high selectivity of coordination intermediates generated between Ca 2+ and phenolic compounds with hydroxyl and methoxy groups towards the parent phenol, the reaction intermediate between each of 4-ethylguaiacol, guaiacol, eugenol and Ca 2+ was used as the extracting agents. Influencing parameters investigated were the sequence of extracting three phenolic compounds from the model bio-oil, the optimal extraction conditions, and the Ca 2+ distribution in the original extracting agents at the end of the reaction. The optimal extraction sequence was determined to be 4-ethylguaiacol, eugenol and guaiacol through the evaluation of multiple experiments changing their addition sequence. Under the conditions of 25 °C reaction temperature, 45 min reaction time, and pH value of 7, the extraction efficiency of 4-ethylguaiacol, eugenol and guaiacol from bio-oil reached 60.6 %, 46.1 % and 60.7 %, respectively. Meanwhile, the purity of these three phenolic compounds achieved 62.4 %, 90.0 % and 89.0 %.
研究领域:
化学工程
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生物质能
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产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
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IF 4
Jiaxin Du,Jiapei Xi,Xiao Chen,Hailan Sun,Lei Zhong,Qiping Zhan,Liyan Zhao
Shiitake mushroom is a highly nutritious and delicious edible fungus with a unique flavour , making it popular among consumers globally. In this study, the effects of different extraction methods on the release of non-volatile flavour substances in shiitake mushroom were investigated using e-tongue, high performance liquid chromatography (HPLC), and principal component analysis (PCA). The results of pretreatment showed that hot air drying at 60 ℃ had higher umami intensity. On the basis of it, enzymatic hydrolysis was found to be beneficial for the release of soluble sugars (alcohols), organic acids , and free amino acids , especially umami, sweet amino acids like Glu, Ser, and Thr. Double enzymatic hydrolysis had higher umami intensity. On the other hand, cooking was beneficial to the release of 5′-nucleotides, with 5′-AMP and 5′-GMP being key flavour substances in high-pressure cooking. In conclusion, enzymatic hydrolysis can increase the ratio of umami and sweet amino acids, and high-pressure cooking is the best way to promote the release of 5′ -nucleotides. The findings of this study provide a theoretical foundation for the development of shiitake mushroom condiment .
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
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IF 5.162
Xuan Zhang,Jiawei Lin,Fang Pi,Tao Zhang,Chao Ai,Shujuan Yu
This work aimed to extract gelatinous chicory root pectin (CRP) and evaluated the rheological behavior of the dispersions and gels. CRP was extracted by citric acid (CEP), alkaline (AEP), ammonium oxalate (OEP) and sodium citrate (SEP). The yield, molecular weight (Mw) and the degree of esterification (DE) of pectin samples varied from 8.8 to 14.8% (w/w), 204 to 336 k Da and 4.0 to 47.4%, respectively. AFM studies showed self-organize on mica of CEP, revealing a random coil conformation due to the interaction of multiple branching, whereas, AEP exhibited long linear filamentous structures. The flow behavior study verified the pseudoplastic character of CEP and SEP at 25 °C, while OEP and AEP belonged to dilatant fluid, besides, a closed hysteresis loop was observed when the CEP concentration increased to 1.5%. OEP gel was thermo insensitive and stiff, AEP gel presented most sensitive to calcium ion but more brittle, and SEP was observed a weak syneresis in spite of the poor gelation property. The texture analysis indicated OEP gel had a superior firmness and chewiness. These findings demonstrated that CRP may be attractive as a thickener or gelling agent to modulate textures of sugar-free and calcium content food.
研究领域:
食品科学
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高分子化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AOAC INTERNATIONAL
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2020
·Vol. 104
·期 2
·479-484
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IF 1.51
Lan Tao,Yu Congcong,Li Ren,Ma Zheng,Xi Xingjun,Chu Qiao
BackgroundSolanesol is an important pharmaceutical intermediate raw material, mainly used to synthesize coenzyme Q10, vitamin K2. It can be found prominent in potato stems and leaves. But now potato stems and leaves are always abandoned or discarded as they are not suitable for use as feed in aquaculture or other purposes. These agricultural waste resources can be reutilized as the corresponding extracts.ObjectiveTo develop a simple and standardized method for the detection of total solanesol in potato leaves and its extracts.MethodsN-hexane was chosen as the extraction solvent for three times in the solanesol extraction from potato leaves. HPLC-MS was used for the detection.ResultsThe LOQ was 0.3 µg/g and the linear range was from 0.1 to 50 µg/mL. The precision and stability were evaluated by the relative standard deviations (RSDs) of three samples (potato leaves, Extract-1, Extract-2) for interday and intraday. The accuracy of the method was evaluated by the recoveries of three different spiked concentrations of solanesol for three samples, and results showed it ranged from 80.7% to 99.0% with RSDs less than 8.7%.ConclusionsThe method we established can provide a simple and standardized way for the extraction and detection of total solanesol.HighlightsThe work laid a foundation for the resource reutilization of potato stem and leaf.
研究领域:
植物化学
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分析化学
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药用植物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
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IF 3.909
Yujiao Sun,Shuting Hou,Shuang Song,Bao Zhang,Chunqing Ai,Xuefeng Chen,Ning Liu
This study comparatively evaluated the effects of different extraction methods on yield, structural features and antioxidant activities of Laminaria japonica ( L. japonica ) polysaccharides . Results showed that acid, water and alkaline extracted L . japonica polysaccharides (LJP-CA, LJP-W and LJP-A, respectively) differed significantly. Among three polysaccharides, LJP-W showed the highest viscosity with high uronic acid content and average molecular weight, whereas LJP-CA showed the lowest viscosity with low uronic acid and high sulfate content with moderate average molecular weight. LJP-CA was mainly composed of fucose , mannose and galactose , but the proportion of glucose was greatly increased in LJP-W and LJP-A. LJP-W had an even and smooth sheet-like appearance, while LJP-CA and LJP-A exhibited irregular and rough fragments or particles with stronger antioxidant activities. These results suggest that acid and alkali would propose an improved process for polysaccharide preparation from L . japonica and to exploit its potential application as a functional ingredient in food application.
研究领域:
食品科学
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海洋生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2017
·
IF 4.529
Yuyu Jia,Lin Wang,Li Ma,Zhaoguang Yang
A comparative study between microwave assisted and sonication methods was carried out to extract arsenic (As) species in shellfish samples using different extractants. Six As species including arsenite [As(III)], arsenate [As(V)], monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenobetaine (AsB) and arsenocholine (AsC) were simultaneously separated and determined by the HPLC–ICP-MS method. The microwave assisted method exhibited higher efficiency than sonication, especially using diluted HNO 3 as extractant. By compromising extraction efficiency, pretreatment time and stability of As species, the microwave assisted method using 1% HNO 3 at 100 °C for 1.5 h was selected to extract As from real samples. The proposed method has been applied to extract and determine As species in shellfish samples. The result of correlation analysis indicated that the proportion of AsB in the shellfish samples was decreased with total As concentration increasing due to the biotransformation threshold from inorganic As to AsB.
研究领域:
环境科学
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分析化学
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食品安全
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产品关联
DOI
PubMed
高影响因子
CHEMICAL GEOLOGY
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2022
·
IF 4.685
Ming Zhang,Ying Xu,Ke-Qing Xiao,Chun-Hui Gao,Shuang Wang,Di Zhu,Yichao Wu,Qiaoyun Huang,Peng Cai
Extracellular polymeric substances (EPS) exuded by microbes are found in diverse habitats, including soil. The quantification and characterization of EPS are essential prerequisites for understanding the ecological functions of extracellular products in soil. However, few studies have conducted in-depth profiling of soil EPS extraction steps and chemical characteristics. Here we examine the C and N contents, biopolymer contents, and optical properties of EPS involving three soil pre-treatment methods (moist soil, moist soil pre-incubated, and air-dried soil pre-incubated) and simultaneously track the compositional changes during the two-step extraction process (extract with CaCl 2 in Step 1 and phosphate buffer solution (PBS) with cation exchange resin (CER) in Step 2). The optical properties of soil EPS are investigated using ultraviolet-visible adsorption (UV–Vis), excitation-emission-matrix fluorescence (EEM), and fourier transform infrared (FTIR) spectroscopy coupled with multivariate analytical methods such as parallel factor analysis (PARAFAC), principal component analysis (PCA), and correlation analysis. Results show that Step 2 releases significantly more dissolved organic carbon and biopolymers (polysaccharides, proteins, and uronic acids) from soils. UV–Vis and EEM spectroscopy suggest that the fractions extracted in Step 2 exhibit higher mean aromaticity, molecular weight , and humification degree than those extracted in Step 1. When using the standard method to extract EPS from moist aggregates (Step 2), EPS has a distinct fluorescence profile, rich in tryptophan- and tyrosine-like signals. Reassuringly, the fluorescence-profiles found in pre-extracts (Step 1) are very different - except where i) aggregates have an experimental drying step added, and ii) pure water is used as a pre-extractant. Drying of aggregates is thus problematic, and the use of pure water as an alternative pre-extractant is not advisable. Soil treatments have significant effects on the qualities of the various extracts which are detected by both the FTIR-PCA and EEM-PCA analyses. EEM fluorescence profiles of EPS suggest that both tryptophan-like and tyrosine-like substances are key components of the EPS extracted from all soils regardless of pretreatment. Our findings indicate that chemical characterization of soil EPS combined with spectral-chemometric approaches can be applied to identify and characterise the extracellular responses of soil microbes and thus help reveal the ecological functions of extracellular products in soils.
研究领域:
土壤生物学
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环境科学
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微生物生态学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Mengqiang Wang,Li Jia,Xun Jiao,Tangwei Zhang,Ming Xiao,Miao Wang,Shanshan Wang,Lufei Zheng,Jing Wang,Yongxin She,A.M. Abd EI-Aty
Fish farming plays a vital role in providing food, nutrition, and employment globally. However, this industry faces security challenges, necessitating the use of fungicides and preservatives, such as bronopol, to increase product yields. Bronopol (2‑bromo-2-nitropropan-1,3-diol; CAS:52–51–7) is widely used in various fields, including food production, cosmetics, and, more recently, aquaculture. Currently, there is a limited number of techniques available for detecting bronopol in aquaculture products. This is primarily due to bronopol's instability, susceptibility to degradation, and tendency to form precipitates that pose challenges in extraction from aquaculture products. For this issue, this study presents a comprehensive method for detecting bronopol content in aquaculture tissues using liquid chromatography-tandem mass spectrometry (LC‒MS/MS). The methodology was optimized, involving extraction with Cu-Zn precipitant, cleanup using a small HLB column, separation on a T3 column, and gradient elution with water and acetonitrile mobile phases. The quantitative approach was employed without the use of an internal standard, following the external standard method. The spiked recoveries at 3 fortification levels (0.1, 0.2, and 1 mg/kg) ranged from 87.1 % to 108.1 % with relative standard deviations RSD ≤ 9.0 %. By applying this method to fresh fish, shrimp, crab, and shellfish samples from a local supermarket, no residues of bronopol were detected, ensuring the reliability of the results. The simplicity, rapidity, and high sensitivity of the method make it a suitable alternative to conventional techniques for bronopol detection. Moreover, the successful validation of the method's recovery and precision supports its potential application in monitoring and preventing the misuse of bronopol in aquaculture, thereby safeguarding aquaculture product quality and protecting public health.
研究领域:
食品科学
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分析化学
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水产养殖
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2018
·
IF 2.831
Jun Cao,Luyao Chen,Mohan Li,Fuliang Cao,Linguo Zhao,Erzheng Su
Proanthocyanidin (PAC) holds strong free radicals scavenging ability and is widely used as oxygen free radical scavenger. Huge demand, safety and economic aspect are forcing the PAC extraction to explore new resource and greener solvent. An efficient extraction method of PAC from Ginkgo biloba leaves was established by employing deep eutectic solvent (DES)-water mixture. After a rational design, a DES-water mixture (named as Ch-M 55 ) was chosen as the extraction solvent, which was prepared from choline chloride and malonic acid at a molar ratio of 1:2 with water addition of 55% (w/w). The main factors affecting the extraction yield were statistically optimized using a central composite design combined with response surface methodology (RSM). The optimal conditions were obtained as follows: extraction temperature of 65 °C, extraction time of 53 min and Ch-M 55 to solid ratio of 10.57:1 (v/w, mL/g). The PAC extraction yield was 22.19 ± 0.71 mg/g under the optimized conditions, which was much higher than those of conventional organic solvents. The antioxidant activity of PAC extracted by Ch-M 55 was similar to that of 70% acetone, but a little less than 70% methanol and 70% ethanol. The differences in constituent and polymerization degree of PAC extracted by different solvents might account for the difference in antioxidant activity. In consideration of biodegradability and pharmaceutical acceptability, the Ch-M 55 -based extraction method is obviously efficient, green and ecofriendly. Extraction of PAC from Ginkgo biloba leaves also provides a new PAC resource.
研究领域:
植物化学
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天然产物开发
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药用植物研究
✓ 已验证
DOI
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 3
·1668-1678
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IF 3.361
Dechao Chen,Sai Ma,Xiaofan Zhang,Xuedong Wang,Ming Gao,Jieyi Li,Huili Wang
The reported ionic liquid (IL)-based magnetic effervescent tablets are a result of direct addition of ILs and magnetic nanoparticles (MNPs). In effervescent reaction-enhanced microextraction procedures, the dissociation between ILs and MNPs easily leads to loss of ILs due to aqueous solubility, thereby decreasing the extraction efficiency. Herein, we attached a hydrophilic IL ([BMIM]Br) onto the surface of NiFe2O4@SiO2@polyaniline (NiFe2O4@SiO2@PANI-IL) to prepare novel core–shell-like multi-layer nanocomposites. Magnetic effervescent tablets were composed of Na2CO3 as an alkaline source, tartaric acid as an acidic source and as-synthesized nanocomposites as an extractant. The nanocomposites were used in an effervescent reaction-enhanced magnetic solid-phase extraction (ERMSE) for the extraction of four organophosphorus pesticides (OPPs) in fruit juices prior to HPLC-DAD detection. Under optimized conditions, this method provided low limits of detection (0.06–0.17 μg L−1), high recoveries (80.6–97.3%) and excellent precision (1.1–5.2%) for OPP quantification in five fruit juices. Notably, the three-layer core–shell nanocomposites were efficiently recycled for at least eight extraction cycles with a recovery loss of <10%. The novelty of this study lies in: (1) for the first time, the ILs-based hybrid magnetic nanocomposites were prepared with appropriate pore size/volume and more active sites for OPPs; (2) the combination of the nanocomposites with effervescent tablets realizes rapid dispersion of CO2 bubbles, and convenient magnetic separation/collection into one synchronous step; and (3) due to there being no requirement of electrical power, it is feasible for use in field conditions. Thus, the ERMSE method has excellent potential for conventional monitoring of trace-level OPPs in complex fruit juice matrices.
研究领域:
分析化学
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环境科学
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食品科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）