SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Hai-Bo Wang,Bei-Bei Tao,An-Li Mao,Zhong-Liang Xiao,Yan-Ming Liu
The exploration of turn-on fluorescent method for sensitive detection of tetracycline is still challenging. This paper reports a kind of specific structure of self-assembled copper nanoclusters (CuNCs), greatly enhancing the fluorescence emission intensity of tetracycline (TC). Fluorescence enhancement is mainly ascribed to that tetracycline can defuse into the compatible space of special structure of self-assembled CuNCs, and intramolecular motion of TC can be effectively restricted, leading to fluorescence emission enhancement of TC. The assay enables TC to be detected with high sensitivity and linear response that covers the 200 nM to 50 μM TC concentration range with a 40 nM limit of detection, which is lower by 50-fold than the method without self-assembled CuNCs. Furthermore, the strategy is further applied for determining TC contents in milk and urine samples.
研究领域:
纳米技术
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分析化学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 13
·10594
·
IF 5.6
Zanlin Chen,Haiming Li,Miaojia Xie,Fengguang Zhao,Shuangyan Han
This study proposes a label-free aptamer biosensor for the sensitive detection of malachite green(MG) using gold nanoparticles/multi-walled carbon nanotubes @ titanium dioxide(AuNPs/MWCNTs@TiO2). The nanocomposite provides a large surface area and good electrical conductivity, improving current transfer and acting as a platform for aptamer immobilization. The aptamer and the complementary chain(cDNA) are paired by base complementary to form the recognition element and fixed on the AuNPs by sulfhydryl group, which was modified on the cDNA. Since DNA is negatively charged, the redox probe in the electrolyte is less exposed to the electrode surface under the repulsion of the negative charge, resulting in a low-electrical signal level. When MG is present, the aptamer is detached from the cDNA and binds to MG, the DNA on the electrode surface is reduced, and the rejection of the redox probe is weakened, which leads to an enhanced electrical signal and enables the detection of MG concentration by measuring the change in the electrical signal. Under the best experimental conditions, the sensor demonstrates a good linear relationship for the detection of MG from 0.01 to 1000 ng/mL, the limit of detection (LOD)is 8.68 pg/mL. This sensor is stable, specific, and reproducible, allowing for the detection of various small-molecule pollutants by changing the aptamer, providing an effective method for detecting small-molecule pollutants.Keywords:aptamer;cyclic voltammetry;potassium hexacyanoferrate;sensitive detection;differential pulse voltammetry
研究领域:
分析化学
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环境科学
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生物传感器技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemosensors
·
2023
·Vol. 11
·期 9
·482
·
IF 4.2
Chunmao Zhu,Qi Wu,Fanshu Yuan,Jie Liu,Dongtian Wang,Qianli Zhang
In this paper, a MnO2nanowire (MnO2-NW) modified carbon paper electrode (CP) was developed as a novel electrochemical sensor for the sensitive determination of tetrabromobisphenol A (TBBPA). The MnO2nanowire was prepared by a hydrothermal synthesis method, and the morphology and structure of MnO2were characterized using scanning electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The electrochemical performance of TBBPA on MnO2-NW/CP was investigated by cyclic voltammetry, and the result confirmed that MnO2-NW/CP exhibited excellent sensitivity for the determination of TBBPA due to the high specific surface area and good electrical conductivity of the nanowire-like MnO2. Moreover, the important electrochemical factors such as pH value, incubation time and modified material proportion were systematically studied to improve the determination sensitivity. The interferences from similar structure compounds on TBBPA have also been investigated. Under the optimal conditions, MnO2-NW/CP displayed a linear range of 70~500 nM for TBBPA with a detection limit of 3.1 nM. This was superior to some electrochemical methods in reference. The work presents a novel and simple method for the determination of TBBPA.
研究领域:
分析化学
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Mengyao Mu,Shiping Zhu,Yanmei Gao,Ning Zhang,Youmei Wang,Minghua Lu
The sensitive detection of polychlorinated biphenyls (PCBs) is crucial for protecting the environment and human health. Herein, we constructed a Materials Institute Lavoisier 88B (MIL-88B)-on-University of Oslo 66 (UiO-66) composite (MIL-on-UiO) with a unique nanoflower morphology, in which highly stable UiO-66 is the precursor, with MIL-88B grown on its surface. MIL-on-UiO was used as a fiber coating for headspace solid-phase microextraction to enrich PCBs. Experimental results demonstrated that MIL-on-UiO provided better enrichment performance for PCBs than single components due to multiple interactions, including π–π stacking, halogen bonding, pore-filling, and steric hindrance effects. The method established using the MIL-on-UiO-based SPME fiber coating provided a good linear relationship in the range of 0.001–50 ng·mL −1 , with limits of detection ranging from 0.0002 to 0.002 ng·mL −1 and enrichment factors between 3530 and 7420. In addition, the method was used to detect trace PCBs in water and orange juice achieving satisfactory recoveries (81%–111%).
研究领域:
环境科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 34
·7792-7800
·
IF 5.344
Rui Lv,Enhui Wu,Renfei Wu,Wen Shen,Chongqing Ma,Rui Shi,Ruochen Guo,Mingwang Shao,Jian Liu
There is increasing demand for anti-doping drug monitoring in sports and food safety checks by developing sensitive and fast analytical methods. Here we report the development of hybrid Ir/SiNW as a new MALDI matrix for the detection of small molecules. This matrix is characterized by sufficient UV absorption, low-noise background, and high efficiency in ionization of small molecules. Sensitive detection of clenbuterol (LOD: 0.18 pmol) and a variety of other small molecules has been achieved using the Ir/SiNW matrix with a reproducible performance. Compared to the individual components separately, the matrix of hybrid Ir/SiNW synthesized via in situ growth can promote the MS signal intensity by up to 10 fold under identical experimental conditions. We provide a unique mechanism for the high performance of the hybrid Ir/SiNW matrix with the characteristic properties of hydrogen atom transfer and enhanced protonation at the interface of the hybrid nanostructures. Our approach of using a hybrid Ir/SiNW matrix enables detection of clenbuterol quantitatively in complicated biological samples and in vivo experiments, promising a useful tool for food security and anti-doping drug monitoring in sports.
研究领域:
分析化学
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食品科学
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运动医学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers of Chemical Science and Engineering
·
2022
·Vol. 17
·期 1
·15-23
·
IF 4.803
Qu Qi,Zeng Chuan,Huang Jing,Wang Mengfan,Qi Wei,He Zhimin
Recently, various semiconductor/metal composites have been developed to fabricate surface-enhanced Raman spectroscopy substrates. However, low metal loading on semiconductors is still a challenge. In this study, cystine was introduced to increase the accumulation of gold nanoparticles on zinc oxide, owing to the biomineralization property of cystine. Morphological analysis revealed that the obtained ZnO/Au/cystine composite not only had a higher metal loading but also formed a porous structure, which is beneficial for Raman performance. Compared with ZnO/Au, the ZnO/Au/cystine substrate displayed a 40-fold enhancement in the Raman signal and a lower limit of detection (10 −11 mol·L −1 ) in the detection of rhodamine 6G. Moreover, the substrate has favorable homogeneity and stability. Finally, ZnO/Au/cystine displayed excellent performance toward crystal violet and methylene blue in a test based on river water samples. This study provided a promising method to fabricate sensitive semiconductor/noble metal-based surface-enhanced Raman spectroscopy substrates for Raman detection.
研究领域:
材料科学
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分析化学
✓ 已验证
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Kai Gao,Song Cui,Sibo Liu
In terms of outcome prediction in patients with inflammation, albumin and C-reactive protein (CRP) at serum levels have been considered to constitute a practical and reliable scoring system. In the present work, highly sensitive detection of C-reactive protein (hs-CRP) was achieved via a new strategy using a quartz crystal microbalance (QCM) immunosensor. A linear relationship was found between the QCM response and CRP concentration (0.04 - 30 μg/mL), with a low limit of detection (LOD) maintained via an amplification process.
研究领域:
医学诊断
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生物传感技术
✓ 已验证
DOI
高影响因子
ANALYST
·
2021
·Vol. 146
·期 9
·2862-2870
·
IF 4.616
Weidi Sun,Xue Han,Fengli Qu,Rong-Mei Kong,Zilong Zhao
The development of sensitive methods for alkaline phosphatase (ALP) activity analysis is an important analytical topic. Based on the stimulus-responsive lanthanide coordination polymer, a simple ratiometric fluorescence sensing strategy was proposed to detect ALP activity. A carbon dot (CD) doped fluorescent supramolecular lanthanide coordination polymer (CDs@Tb-GMP) was prepared with Tb3+ and the ligand guanine single nucleotide (GMP). To construct a ratiometric fluorescence biosensor, the fluorescence of Tb-GMP was used as a response signal, and the fluorescence of CDs was used as a reference signal due to its good stability. When excited at 290 nm, the polymer network Tb-GMP emits characteristic fluorescence at 545 nm, while the CDs encapsulated in the polymer network emit fluorescence at 370 nm. After adding ALP to the system, the substrate GMP can be hydrolyzed by ALP, resulting in the destruction of the polymer network. Accordingly, the fluorescence of Tb-GMP significantly decreased, while the fluorescence of CDs slightly increased due to their release from the polymer network. By comparing the relationship between the fluorescence intensity ratio of the two signals and the concentration of ALP, sensitive detection of ALP could be achieved with the linear range from 0.5 to 80 U L−1 and a detection limit of 0.13 U L−1. Furthermore, the proposed ratiometric sensing system was applied to the detection of ALP in human serum samples with desirable results, indicating potential application in clinical diagnosis.
研究领域:
分析化学
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生物医学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2022
·Vol. 15
·期 4
·529-536
·
IF 3.532
Zian Wang,Zhiwei Liu,Tian Guan,Xi Zeng,Runlin Shen,Zhaodong Li,Yi Lei,Zhenlin Xu,Zhili Xiao,Hongtao Lei,Dingqiang Huang
With the popularity of herbal tea in China, many food fraudsters have added illegal drugs to herbal tea to enhance its functions, among which aminopyrine is widely abused as an antipyretic and analgesic. Presently, there is no immunoassays for aminopyrine, and it is difficult to achieve real-time detection in the field. Based on a polyclonal antibody of aminopyrine with high specificity and sensitivity, an optimal combination of coating antigen/antibody was obtained by screening different coating antigens. On this basis, a sensitive ic-ELISA method was established to detect aminopyrine in herbal tea. The detection limit of the ic-ELISA was 0.18 ng mL−1, which was much lower than the 100 ng mL−1 required as a standard. The method had good consistency with LC-MS in the detection of actual samples and could be used as a reliable method for the detection of aminopyrine in herbal tea.
研究领域:
食品科学
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分析化学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Qi Guo,Xiaorong Huang,Yajuan Huang,Zhaowei Zhang,Peiwu Li,Li Yu
Aflatoxin B 1 (AFB 1 ) is a prominent food contaminant known for its high toxicity. Rapid, low-cost, and sensitive detection of AFB 1 is an urgent demand for food safety risk control. In the traditional enzyme-linked immunosorbent assay (ELISA) method for AFB 1 detection, horseradish peroxide (HRP) is high-cost and possesses poor environmental stability. In this study, a direct competitive single-atom nanozymes (SAzymes) -linked immunosorbent assay was developed based on Fe-N-C SAzymes. The proposed methods exhibited excellent sensitivity, rapid detection, fabrication, and low cost. Under the optimal conditions, the limit of detection of the new assay was 3.3 pg mL −1 , with a quantitative relationship between 0.0084 and 0.358 ng mL −1 . In addition, the developed method presented good recoveries in spiked samples, ranging from 84.50% and 103.75%, with coefficients of variation below 10%. Furthermore, the developed detection method showed good consistency (R 2 = 0.9852) with the reference method, liquid chromatography/mass spectrometry (LC-MS/MS). To our knowledge, this is the first report about single-atom nanozymes-based ELISA for AFB 1 determination. This work provides a feasible strategy for the rapid and sensitive detection of food contaminants using SAzymes.
研究领域:
食品科学
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生物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2023
·
IF 6.7
Xiaoming Li,De Zhang,Le Wang,Lu Yin,Xiaohua Qi,Minqiang Zou,Qiang Chen,Zhi Yu,Pei Liang
Sensitive and reliable interfacial self-assembled nanomembranes are widely used for the detection of chemical contaminants in food and environment. This study developed a high-performance Surface-Enhanced Raman Spectroscopy (SERS) analytical platform by self-assembling Au nanoparticles (AuNPs) at the three-phase liquid–liquid interface. The assembled nanoparticles arrays were immersed in halogen ions solution and the nanoparticle gaps were further shrunk through electrostatic interactions, resulting in a large-scale array of closely packed nanoparticles. The average gap between adjacent nanoparticles was less than 3 nm, where dense SERS ‘hot spots’ were generated and used for highly sensitive detection . The homogeneity of the self-assembled nanoparticle arrays under different conditions was still guaranteed despite the reduced integration time and the number of times in the mapping face sweep of the Au arrays. The improved high-throughput fabrication of self-assembled nanoparticle arrays in this way holds great promise for the development of a sensitive and reliable SERS platform for chemical contaminant monitoring.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Bin Shang,Yanbing Wang,Peng Yang,Bo Peng,Ziwei Deng
Surface-enhanced Raman scattering (SERS) sensor allows for fingerprinting and label-free molecular detection with a high sensitivity, but the simple fabrication of SERS active substrates with a well-control over the SERS performance, e.g. an ultra-high sensitivity, superior reproducibility and long-term stability remain a great challenge. Here, we introduce an easy strategy for synthesizing polydopamine-Ag microbowl/nanoparticle array (PDA-Ag MNA) substrates with a tunable superhydrophobic surface, applicable for ultra-highly sensitive SERS detection. First, the periodically dented PDA films are prepared by polymerizing dopamine onto 2D polystyrene colloidal crystals formed at the liquid/air interface, and followed with an in-situ reduction of Ag ions to Ag nanoparticles after removing polystyrene. The surface morphology, i.e. microbowl/nanoparticle structure, is well-controlled, therefore, allowing that the SERS activity of the substrate is under control. Inspired by the superhydrophobic hierarchical surface of the rose petals, this analogous MNA surface is subsequently endowed to be superhydrophobic. This way, the SERS detection of ultra-dilute solute (10 −14 M) can be achieved by concentrating analytes within a localized region. Moreover, this substrate also shows a superior reproducibility in result and durability in use. This strategy exhibits a realistic paradigm for further improving the sensitivity of the SERS technique.
研究领域:
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
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