INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Jing Liu,Hao Chen,Yan Lv,Lijun Yang,Wenjie Wang,Jianying Huang,Dandan Zhang
The mycotoxin-producing fungi contamination of foodstuffs and agricultural commodities is a serious problem. In this study, the efficacy of acetic acid (AcA), chitosan (CS), and CS with extra AcA (CS/extra AcA) against Aspergillus flavus ( A. flavus ) in vitro and in vivo were investigated. Results showed that CS/extra AcA strongly inhibited the mycelial growth and aflatoxins production of A. flavus at lower concentrations than CS (MIC > 16.0 mg/mL) or AcA (MIC: 0.31 %). Significant inhibition was achieved in the presence of 4.0/0.12 and 8.0/0.08 CS (mg/mL)/extra AcA (%). CS/extra AcA exhibited remarkable antifungal activities based on the results of spore germination inhibition, abnormalities of spore morphology, disruption of cell membrane, mitochondrial dysfunction, and induction of apoptosis of hyphae. Moreover, pathogenicity test in peanut kernels showed that 8.0/0.16 CS (mg/mL)/extra AcA (%) could effectively inhibit the fungal infection and aflatoxin B 1 production. The efficacy of CS/extra AcA is possibly due to the synergy of the antifungal property of CS, and undissociated AcA. The findings referred that CS/extra AcA is safe, efficient, and economical, and it could be used as a promising way to control A. flavus contamination in agro-foods preservation.
研究领域:
食品科学
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微生物学
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DOI
PubMed
高影响因子
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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2014
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IF 1.869
Jianwei Dong,Lixing Zhao,Le Cai,Haixian Fang,Xiuhua Chen,Zhongtao Ding
The tubers of Bletilla formosana were fermented with eight plant pathogen fungi, respectively, and antioxidant activities and total phenolic content (TPC) of the crude extracts of fermented products and non-fermented products were investigated. The antioxidant activities were evaluated in three different test systems [DPPH, ABTS radical-scavenging activity, and ferric reducing-antioxidant power (FRAP)]. It was found that the extract of Helminthosporium maydis fermented B. formosana (FBF) possessed the highest TPC and exhibited a significant antioxidant activity compared with non-fermented product and other fermented products. Correlation analysis between antioxidant activities and TPC was also investigated. The good correlation between antioxidant activities and TPC revealed that the phenolic compounds might be the major contributors for the high antioxidant activities of the fermented B. formosana . Two phenolic compounds, curvularin and dehydrocurvularin, were isolated from H. maydis FBF, which had never been reported from plant of orchidaceae or H. maydis . Curvularin exhibited significant antioxidant activities, and was also present at a high concentration (0.373 mg/mg extract sample), implying an important role for the antioxidant activity of H. maydis FBF. This study suggested that proper fermentation processing could improve TPC and antioxidant activities of B. formosana .
研究领域:
植物学
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药用植物研究
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食品科学
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DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2023
·
IF 4.5
Kui Chen,Jingkang Wang,Wenbo Wu,Huiting Shan,Hongtu Zhao,Na Wang,Ting Wang,Xin Huang,Hongxun Hao
Organic crystals with flexibility have been a research hotspot due to their huge potential in flexible optoelectronics . However, most organic crystals are brittle, which greatly restricts their application in flexible smart materials. Herein, a flexible crystal with multi-faced elastic bending and plastic twisting was reported. Even after undergoing severe twisting deformation, it could be freely bent in any direction due to the twisted shape, thus achieving the transition to three-dimensional (3D) elastic bending. The mechanisms of multiple flexibilities were investigated by detailed experimental analysis and theoretical calculations. Especially, the mechanism of twistable crystals with elasticity was well revealed by microfocus spectroscopy and theoretical calculation, which has not been reported before. It was found that the twisting of organic crystals can be attributed to the molecular rotation without spatial hindrance, abundant weak intermolecular interactions and different orientations of the intermolecular interactions. Finally, taking advantage of its flexibility, its potential application in flexible active and passive optical waveguides was demonstrated.
研究领域:
材料科学
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光学
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理论计算
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DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 18
·3420
·
IF 5.2
Minyu Cai,Liyan Huang,Sashuang Dong,Nanxin Diao,Weilian Ye,Zhiye Peng,Xiang Fang
Summer green tea (SGT) has a low cost and high annual yield, but its utilization rate is limited due to suboptimal quality. The aim of this study is to enhance the flavor of SGT using fermentation withA. nigerRAF106 while examining changes in its metabolites during this process. The results revealed an elevation in the content of alcohol, alkanes, and nitroxides in tea leaves following the process of fermentation. The predominant volatile compounds identified in tea leaves after undergoing a 6-day fermentation period were linalool, (Z)-α, α, 5-trimethyl-5-vinyltetrahydrofuran-2-methanol, (E)-linalool oxide (furan type), linalool oxide (pyran type), and theapyrrole. These compounds exhibited significant increases of 31.48%, 230.43%, 225.12%, 70.71%, and 521.62%, respectively, compared to the non-fermented control group (CK). The content of non-ester catechins, soluble sugars, and total flavonoids reached their peak on the 4th day of fermentation, exhibiting significant increases of 114.8%, 95.59%, and 54.70%, respectively. The content of gallic acid and free amino acids reached their peak on the 6th day of fermentation, exhibiting significant increases of 3775% and 18.18%, respectively. However, the content of ester catechin decreased by 81.23%, while caffeine decreased by 7.46%. The content of lactic acid, acetic acid, and citric acid in tea after fermentation was 421.03%, 203.13%, and 544.39% higher than before fermentation, respectively. The present study offers a fresh approach for the advancement of SGT.Keywords:summer green tea;Aspergillus niger;fermentation;flavor
研究领域:
食品科学
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微生物学
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PubMed
高影响因子
Science Advances
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2025
·Vol. 11
·期 36
·
IF 12.5
Zeping He,Boning Sun,Hui Lu,Xidi Sun,Zhangsheng Xu,Yegang Liang,Qiuchun Lu,Yang Yu,Kaiyu Hu,Swapnadeep Poddar,Wenqiang Wu,Wenchao Gao,Xun Han,Zhiyong Fan,Caofeng Pan
The human visual system’s real-time focus-tunable imaging capability has inspired curved imaging system development. However, curved image sensors mimicking the human retina typically lack tunable curvature to match the curved Petzval surface throughout the focus-tunable range. Here, we propose a focus-tunable real-time curved imaging system based on a tunable-curvature perovskite curved image sensor. Using parylene C film for waterproofing and encapsulation, the ultrathin perovskite curved image sensor with the hierarchical mesh design is demonstrated, which conforms to spherical surfaces of different radii while maintaining mechanical stability during deformation. The image sensor exhibits low detection limit (10 nanowatts per square centimeter) and long-term stable dynamic photoresponse. Combining the image sensor with a tunable lens achieves clear images at different distances via simultaneously adjusting both sensor and lens radii. Furthermore, through integrating image sensor with a readout matrix and wireless data collection component, the real-time imaging system is demonstrated with real-time focus-tunable imaging capabilities.
研究领域:
光学成像
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传感器技术
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材料科学
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柔性电子学
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生物医学工程
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PubMed
高影响因子
Food Bioscience
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2022
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IF 5.318
Chengtuo Niu,Xianlei Xing,Xiaohong Yang,Feiyun Zheng,Chunfeng Liu,Jinjing Wang,Qi Li
This study aimed to obtain an Aspergillus oryzae strain with high protease activity and to evaluate its potential to apply as starter culture in bean-based fermented foods (BBFFs) production using doubanjiang fermentation as research model. A. oryzae BL18 with higher protease activity than the commonly used starter A. oryzae 3.042 was obtained through two rounds of screening. Moreover, this strain was also proved to be safe to apply in BBFFs fermentation due to the lack of the ability to produce aflatoxin B1 through genomic and experimental analysis. A. oryzae BL18 was optimal to grow under 0–12% salinity, 25–40 °C and pH4-6. During koji -making, A. oryzae BL18 had stronger ability to produce spores (4.19 × 10 8 spores/g dry weight) and to secrete protease (4654.35 U/g) than A. oryzae 3.042 (2.89 × 10 8 spores/g dry weight and 3604.40 U/g). Significantly higher concentrations of umami amino acids and volatile flavor compounds were found in doubanjiang fermentation inoculated by A. oryzae BL18 while better sensory characteristics were observed. Therefore, A. oryzae BL18 might be a promising starter to apply in fermentation of doubanjiang or other BBFFs.
研究领域:
食品科学
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微生物学
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发酵技术
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生物化学
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DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 3
·335
·
IF 5.561
Disha Lu,Xu Wang,Ruijue Su,Yongjian Cheng,Hong Wang,Lin Luo,Zhili Xiao
A novel and efficient immunoaffinity column (IAC) based on bispecific monoclonal antibody (BsMAb) recognizing aflatoxin B1(AFB1) and ochratoxin A (OTA) was prepared and applied in simultaneous extraction of AFB1and OTA from food samples and detection of AFB1/OTA combined with ic-ELISA (indirect competitive ELISA). Two deficient cell lines, hypoxanthine guanine phosphoribosyl-transferase (HGPRT) deficient anti-AFB1hybridoma cell line and thymidine kinase (TK) deficient anti-OTA hybridoma cell line, were fused to generate a hybrid-hybridoma producing BsMAb against AFB1and OTA. The subtype of the BsMAb was IgG1via mouse antibody isotyping kit test. The purity and molecular weight of BsMAb were confirmed by SDS-PAGE method. The cross-reaction rate with AFB2was 37%, with AFG115%, with AFM148%, with AFM210%, and with OTB 36%. Negligible cross-reaction was observed with other tested compounds. The affinity constant (Ka) was determined by ELISA. The Ka (AFB1) and Ka (OTA) was 2.43 × 108L/mol and 1.57 × 108L/mol, respectively. Then the anti-AFB1/OTA BsMAb was coupled with CNBr-Sepharose, and an AFB1/OTA IAC was prepared. The coupling time and elution conditions of IAC were optimized. The coupling time was 1 h with 90% coupling rate, the eluent was methanol–water (60:40,v:v, pH 2.3) containing 1 mol/L NaCl, and the eluent volume was 4 mL. The column capacities of AFB1and OTA were 165.0 ng and 171.3 ng, respectively. After seven times of repeated use, the preservation rates of column capacity for AFB1and OTA were 69.3% and 68.0%, respectively. The ic-ELISA for AFB1and OTA were applied combined with IAC. The IC50(50% inhibiting concentration) of AFB1was 0.027 ng/mL, the limit of detection (LOD) was 0.004 ng/mL (0.032 µg/kg), and the linear range was 0.006 ng/mL~0.119 ng/mL. The IC50of OTA was 0.878 ng/mL, the LOD was 0.126 ng/mL (1.008 µg/kg), and the linear range was 0.259 ng/mL~6.178 ng/mL. Under optimum conditions, corn and wheat samples were pretreated with AFB1-OTA IAC. The recovery rates of AFB1and OTA were 95.4%~105.0% with ic-ELISA, and the correlations between the detection results and LC-MS were above 0.9. The developed IAC combined with ic-ELISA is reliable and could be applied to the detection of AFB1and OTA in grains.Keywords:aflatoxin B1;ochratoxin A;bispecific monoclonal antibody;immunoaffinity column;ic-ELISA
研究领域:
食品科学
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分析化学
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毒理学
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DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2025
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IF 3.5
Yi Chu,Tian-Liang Zhang,Qian Yang,Ming-Xuan Zou,Shuang-Yue Shi,Xue Dong,Rui-Fu Cui,Lei Wang,Jin-Yue Sun,Feng-Xiang Zhang,Xia Wang
BACKGROUND Sesame meal, characterized by high moisture, viscosity and oxidative instability, presents utilization challenges and is frequently relegated to farmland fertilizer – a practice that underutilizes its rich protein content. METHODS This study developed three types of soy sauce using sesame meal supplemented with soybeans. For the first time, a dual-strain co-fermentation system employing Aspergillus oryzae 3.042 and Aspergillus niger AS3.350 was applied to sesame meal. The dynamic evolution of taste and volatile flavor compounds during fermentation was systematically analyzed via electronic tongue technology and gas chromatography–mass spectrometry. The sesame meal soy sauces were benchmarked against four commercial premier-grade products, with flavor profiles evaluated using principal component analysis and orthogonal partial least squares discriminant analysis. Notably, the physicochemical indices of the sesame meal soy sauce complied with Chinese premier-grade standards. RESULTS The dual-strain-fermented soy sauce demonstrated outstanding flavor complexity, sweetness and richness, achieving parity with premier-grade soy sauce. Notably, it contained 2,3,5-trimethylpyrazine and 3-hydroxy-2-butanone, key compounds imparting distinctive roasted grain and creamy flavors, which collectively defined its unique sensory profile. Conclusion This research significantly elevates the utilization value of sesame meal while establishing a viable pathway for its commercial adoption in soy sauce production. © 2025 Society of Chemical Industry.
研究领域:
食品科学
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发酵技术
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风味化学
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分析化学
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生物技术
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2024
·Vol. 59
·期 7
·4880-4891
·
IF 2.6
Meixu Chen,Liling Wang,Xinyi Huo,Qilin Xu,Yaofeng Ying,Linlin Wu,Yifeng Zhou,Jun Huang
Summary In this study, a full factorial design with three temperature levels (20, 30 and 40 °C), three moisture content (MC) levels (8%, 15% and 22%), and four storage times (7, 14, 21 and 28 days) was used to investigate the changes of aflatoxins (AFs), active constituents, and antioxidant activities in Hangbaiju (HBJ, Chrysanthemum morifolium ) after simulated inoculation with Aspergillus parasiticus. The results showed that AFs could be produced in the range of 20~30 °C and 8~22% MC after mould infection, but both mould growth and AFs production were inhibited under the tested MC condition at 40 °C. The analysis of variance ( anova ) indicated that the temperature, time, interaction between the temperature and time, and interaction between MC and time had significant effects on the total aflatoxins (AFTOT) accumulation. Under 20 and 30 °C, AFTOT in inoculated HBJ usually increased with the increase of MC and storage time, while its active constituents and antioxidant activities decreased with the increase of AFTOT under corresponding storage conditions. Pearson correlation analysis suggested that relatively stronger negative correlations were observed between luteolin-7-O-glucoside (LUT7G), total phenolic content (TPC), total flavonoid content (TFC), and AFTOT. Grey correlation analysis (GCA) showed that antioxidant activity was highly linked to the content of active constituents, and the characteristic flavonoid LUT7G exhibited the highest grey correlation degrees (GCDs) in the three antioxidant activities. The AFTOT reached the highest level (31.15 μg kg −1 ) after 28 days of storage at 30 °C and 22% MC; meanwhile, LUT7G, TPC and TFC decreased by 93.66%, 20.77% and 55.50%, respectively, and all the three antioxidant activities indicated more than 25% reduction. This is the first study about the changes and relationships of AFTOT, active constituents and bioactivities in the medicinal food influenced by A. parasiticus under different storage conditions. These results provide important references to prevent AFs production and quality control for HBJ and other medicinal foods influenced by A. parasiticus in storage strategy.
研究领域:
食品科学
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药理学
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微生物学
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植物科学
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DOI
高影响因子
Toxins
·
2020
·Vol. 12
·期 8
·476
·
IF 3.531
Zhimin Zhou,Renkuan Li,Tzi Bun Ng,Yunyun Lai,Jie Yang,Xiuyun Ye
Aflatoxin B1(AFB1) is a known toxic human carcinogen and can be detoxified by laccases, which are multicopper oxidases that convert several environmental pollutants and toxins. In this study, a new laccase that could catalyze AFB1degradation was purified and identified from the white-rot fungusCerrena unicolor6884. The laccase was purified using (NH4)2SO4precipitation and anion exchange chromatography, and then identified as Lac 2 through zymogram and UHPLC-MS/MS based on the Illumina transcriptome analysis ofC. unicolor6884. Six putative laccase protein sequences were obtained via functional annotation. Thelac 2cDNA encoding a full-length protein of 512 amino acids was cloned and sequenced to expand the fungus laccase gene library for AFB1detoxification. AFB1degradation by Lac 2 was conducted in vitro at pH 7.0 and 45 °C for 24 h. The half-life of AFB1degradation catalyzed by Lac 2 was 5.16 h. Acetosyringone (AS), Syrinagaldehyde (SA) and [2,2′ -azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)] (ABTS) at 1 mM concentration seemed to be similar mediators for strongly enhancing AFB1degradation by Lac 2. The product of AFB1degradation catalyzed by Lac 2 was traced and identified to be Aflatoxin Q1(AFQ1) based on mass spectrometry data. These findings are promising for a possible application of Lac 2 as a new aflatoxin oxidase in degrading AFB1present in food and feeds.Keywords:laccase;Cerrena unicolor;aflatoxin B1;biodegradation;detoxification;product;transcriptomeKey Contribution:A product identified to be AFQ1was found from Lac 2-mediated AFB1degradation, indicating that AFB1would be transformed during Lac 2-mediated AFB1degradation.
研究领域:
微生物学
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生物技术
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环境科学
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毒理学
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产品关联
DOI
PubMed
高影响因子
Infection and Drug Resistance
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2023
·
IF 3.9
Lingna Xu,Ren-ai Xu,Dan Zhang,Shanshan Su,Hanyan Xu,Qing Wu,Yuping Li
Background: T helper 17 (Th17) lymphocytes play an important role in Aspergillus adaptive immune response against Aspergillus fumigatus, but there is little attention focused on the different types of immunosuppressive models in which invasive pulmonary aspergillosis (IPA) develops. In addition, the expression levels of signal transducer and activator of transcription 3 (STAT3)/retinoic acid-related orphan nuclear receptor gamma (RORγt)/interleukin (IL)-17A signaling pathway, which is involved in the regulation of Th17 cells, as well as whether there are differences between two types of IPA mice models, remain unknown.Materials and methods: Six to eight weeks old female BALB/c mice were treated with cortisone acetate or cyclophosphamide to establish the immunosuppressive mice models, and then, A. fumigatus inoculum was injected to form the IPA groups and sterile saline was injected to form the control groups. Flow cytometry was performed to measure the proportion of Th17 cells in CD4+ T cells in the peripheral blood, spleen, and lung of the mice. The expression of IL-17A, RORγt, and STAT3 mRNA was detected by real-time polymerase chain reaction. Concentrations of IL-6 in the plasma and bronchoalveolar lavage fluid were measured by enzyme-linked immunosorbent assay.Results: The proportion of Th17 in the peripheral blood and lung tissue in neutropenic IPA mice showed a more significant increase than in non-neutropenic IPA mice (P<0.01). The IL-6 protein also showed the same trend in plasma and bronchoalveolar lavage fluid (P<0.01). Compared with the control groups, the expression of IL-17A at mRNA level in the lung was significantly increased, while RORγt/STAT3 mRNA was significantly decreased in the IPA groups (P<0.01).Conclusion: The expression of RORγt and STAT3 mRNA in the lung tissue in both groups was significantly decreased. IL-17 may play a negative role in the defense against Aspergillus through uprating IL-6.
研究领域:
免疫学
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微生物学
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分子生物学
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病理学
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DOI
PubMed
高影响因子
CHEMOSPHERE
·
2025
·
IF 8.1
Si-yuan He,Wei Wen,Peng Wu,Zhe Meng
Developing a green and efficient technology for decomposing aflatoxin B 1 (AFB 1 ) and ochratoxin A (OTA) in grains has important theoretical value and application prospects for ensuring food safety. A dual Z-scheme ternary heterojunction photocatalyst, Sr 2 MgSi 2 O 7 :Eu 2+ ,Dy 3+ /BiVO 4 /NH 2 -UiO-66 (SBN), was prepared via a self-assembling hydrothermal approach. The SBN heterojunction photocatalyst exhibits round-the-clock photocatalytic activity due to the intrinsic light source of Sr 2 MgSi 2 O 7 : Eu 2+ , Dy 3+ (SMS). Dual Z-scheme mechanism was proposed and validated through detailed analysis of quenching experiments, ESR spectra, band arrangement, and charge transfer processes. The dual Z-scheme mechanism not only promotes the effective transfer and separation of photo generated charge carriers during charge carrier transport, but also maintains significant reduction and oxidation potentials. SBN photocatalyst exhibits stable and persistent photocatalytic performance, significantly enhancing the degradation efficiency of AFB 1 and OTA under all-weather conditions. Experimental data shows that the removal rate of AFB 1 increased from 69.60 % after 60 min of illumination to 83.9 % after 4 h of turning off the light source; At the same time, the removal rate of OTA increased from 64.8 % to 78.1 %. •O 2 − and •OH were identified as key active species in photocatalytic reactions. Based on the analysis of AFB 1 and OTA degradation intermediates and the prediction of degradation sites using density functional theory (DFT), degradation pathways for AFB 1 and OTA were proposed. Collectively, these findings suggest a highly promising practical approach for the detoxification of mycotoxins in grain commodities.
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PubMed
高影响因子
危险品化学品经营许可证（带存储）