SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Penghui Guo,Xiang Gu,Zhaoshuang Li,Xu Xu,Yini Cao,Guoen Yang,Chuntao Kuang,Xingong Li,Yan Qing,Yiqiang Wu
The discharge of heavy metals (HMS) from industrial production has severely damaged the natural environment and human health. To address the challenges posed by heavy metals, a novel almond shell biochar (FeS-CTS@nBC) modified with FeS and chitosan (CTS) was prepared. Scanning electron microscopy and X-ray photoelectron spectroscopy observations revealed a uniform distribution of FeS particles on the biochar. Adsorption thermodynamics experiments showed that the maximum adsorbed amounts of cadmium (Cd), lead (Pb), and chromium (Cr (VI) and Cr (III)) in FeS-CTS@nBC were 85.6, 89.63, 94.2, and 75.62 mg/g, respectively. Results of soil incubation experiments indicated that FeS-CTS@nBC had a desirable immobilization effect on heavy metals, decreasing the bioavailability of Cd, Pb, Cr (VI), and Cr (III) by 29.43 %, 23.93 %, 5.75 % and 5.23 %, respectively. Density functional theory (DFT) calculations, revealed that the oxygen-containing functional groups on the biochar exhibited stronger adsorption capacities for heavy metals. Plant potting experiments indicated that the paddy grew well in the soil remediated with FeS-CTS@nBC. The Cd content in the roots and leaves of the paddy after nBCS2 repair was reduced by 28.01 % and 55.73 %, respectively. Overall, this work provides a promising low-cost method with a simple production process for mitigation of heavy metals from water and soil.
研究领域:
环境科学
·
材料科学
·
植物学
✓ 已验证
DOI
高影响因子
Journal of Advanced Research
·
2025
·
IF 13
Qu-Nan Wang,Chunzhi Wang,Yán Wāng
Introduction The extensive use of 6PPD in tire rubber has led to its increase in atmospheric tire wear particles. 6PPD reacts with ozone to form 6PPD-quinone (6PPD-Q), a respiratory toxicant enriched in tire and road wear particles. Objectives The aim of this study is to decipher the potential sensitive target organs and toxic actions by inhalation exposure to atmospheric 6PPD-Q. Methods This study employed a mouse inhalation exposure model to simulate environmental levels of 6PPD-Q at three concentrations of 0, 0.14, and 14 mg/m 3 . Using a 28-day exposure period followed by an equivalent recovery phase, we systematically evaluated the toxicological targets and effects of subacute exposure. Results The findings revealed that, compared to pulmonary, cardiovascular, and metabolic organ damage, 6PPD-Q-induced neurotoxicity was more persistent and irreversible, particularly characterized by prolonged anxiety-like behaviors. Histopathological analyses of the basolateral amygdala, using Nissl staining and markers of neuronal aging, indicated substantial neuronal degeneration linked to elevated oxidative stress, identifying this region as a critical target of 6PPD-Q neurotoxicity. Transcriptomic analysis uncovered that the expression of Egr1 , a transcription factor crucial for neuronal plasticity, was markedly dysregulated. Findings of significant downregulation at the gene level and an upward trend in protein expression suggest that Egr1 expression is influenced by translational efficiency, epigenetic modifications, and post-translational regulatory mechanisms. Egr1 dysregulation disrupted downstream networks involving solute carrier proteins and calcium-binding proteins, contributing to aberrant neurobehavioral outcomes. Notably, the elevation of Egr1 protein levels in the basolateral amygdala but not in the cerebral cortex highlights the region-specific nature of 6PPD-Q’s neurotoxic effects. Conclusion This study provides the first insights into the neurotoxicity and irreversibility of inhaled 6PPD-Q exposure, paving the way for future research into the long-term neurological consequences and regulatory mechanisms of 6PPD-Q.
研究领域:
毒理学
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神经科学
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环境健康
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS One
·
2018
·Vol. 13
·期 3
·e0193659
·
IF 2.766
Yaoyao Huang,Yang Hu,Lvcun Chen,Tao Yang,Hanfang Huang,Runping Shi,Peng Lu,Chenghua Zhong
Low–cost biosorbents (ginkgo leaf, osmanthus leaf, banyan leaf, magnolia leaf, holly leaf, walnut shell, and grapefruit peel) were evaluated in the simultaneous removal of La 3+ , Ce 3+ , Pr 3+ , Nd 3+ , Sm 3+ , Eu 3+ , Gd 3+ , Yb 3+ , Lu 3+ , UO 2 2+ , Th 4+ , Y 3+ , Co 2+ , Zn 2+ , Ni 2+ , and Sr 2+ from aqueous solutions. In single metal systems, all adsorbents exhibited good to excellent adsorption capacities toward lanthanides and actinides. In a simulated multicomponent mixed solution study, higher selectivity and efficiency were observed for Th 4+ over other metal cations, with ginkgo leaves providing the highest adsorptivity (81.2%) among the seven biosorbents. Through optimization studies, the selectivity of Th 4+ biosorption on ginkgo leaf was found to be highly pH–dependent, with optimum Th 4+ removal observed at pH 4. Th 4+ adsorption was found to proceed rapidly with an equilibrium time of 120 min and conform to pseudo–second–order kinetics. The Langmuir isotherm model best described Th 4+ biosorption, with a maximum monolayer adsorption capacity of 103.8 mg g –1 . Thermodynamic calculations indicated that Th 4+ biosorption was spontaneous and endothermic. Furthermore, the physical and chemical properties of the adsorbent were determined by scanning electron microscopy, Brunauer–Emmett–Teller, X-ray powder diffraction, and Fourier transform infrared analysis. The biosorption of Th from a real sample (monazite mineral) was studied and an efficiency of 90.4% was achieved from nitric acid at pH 4 using ginkgo leaves.
研究领域:
环境科学
·
放射生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2017
·Vol. 41
·期 3
·e12352
·
IF 1
Chang-Wei Zhang,Cheng-Zhang Wang,Ran Tao
Three high purified polysaccharide monomers (GBP11, GBP22, and GBP33) were obtained from enzymatic hydrolysate of Ginkgo biloba leave using DEAE-Sepharose Fast Flow column and Sephadex G-100 column, and their basic structural characterization were investigated by utilizing high-performance liquid chromatography, high-performance ion chromatography, and Infrared Spectroscopy. In addition, antioxidant activities of related polysaccharides were also evaluated. The results showed that the molecular weight of GBP11, GBP22, and GBP33 were 1.944 × 10 3 KDa, 5.167 × 10 3 KDa, and 45.98 KDa, singly, and they were mainly made up of glucose, galactose, arabinose, and rhamnose with a molar ratio of 62.2:1.98:3.84:1.24, 3.57:68.0:4.67:0.36 and 5.34:48.8:1.70:8.97, respectively. Moreover, GBP22, GBP, and GBP11 demonstrated the strongest free radical scavenging activity on DPPH, ABTS, and superoxide anion, showing that they have potential application value on the functional food and cosmetic industry. Practical applications Enzymatic hydrolysate of Ginkgo biloba leaves includes lots of carbohydrate, it will cause eutrophication and environment pollution if it is directly poured out. This research indicates that carbohydrate extracted from enzymatic hydrolysate of Ginkgo biloba leaves are made up of different polysaccharide monomers with various molecular weight and monosaccharide composition. Moreover, these polysaccharide monomers possess appreciating antioxidant activities. Therefore, this research could lay a foundation for the high-value application of polysaccharides in functional food and cosmetic industry, and provide theoretical reference for the treatment of waste water.
研究领域:
植物化学
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食品科学
·
环境科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yongsong Zhang,Na Xia,Zhenzhen Ding,Jingjing Song,Yanan Zhang,Cao Li,Xuesong Huang,Zuoshan Feng
Proteins and polyphenols exhibit distinct biological activities and functional properties. A comprehensive investigation into the formation mechanisms, structures, and functional properties of protein–polyphenol complexes will deepen our understanding of their interactions and establish a theoretical foundation and technical support for development of novel functional foods and pharmaceutical products. The almond protein–phloretin (AP-PHL) covalent complex was synthesized through the covalent binding of hydroxyl radicals to phloretin (PHL), utilizing almond protein (AP) as the raw material. Ultraviolet absorption spectroscopy (UV), fluorescence spectroscopy (FS), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy (RS), transmission electron microscopy (TEM), X-ray diffraction (XRD), and thermogravimetric analysis (TGA) were employed to characterize the AP-PHL complex. Additionally, its properties, including emulsification characteristics and antioxidant activity, were analyzed. The results indicated that the hydrophobic groups in hydroxyl radical-treated AP relocated to a hydrophilic environment and interacted with PHL, thereby forming a stable complex. TEM results indicated that AP formed clusters within the central region of PHL. Additionally, UV and FS analyses revealed that the maximum absorption wavelength of AP-PHL shifted from 287.5 nm to 258 nm and 280 nm, respectively. As the PHL concentration increased, the fluorescence intensity gradually decreased, accompanied by a slight redshift. FTIR and RS analyses revealed that modifications in functional groups (e.g., -CH 3 , =CH 2 , C O, C C, C O) were implicated in the interaction between AP and PHL. Such structural modifications, along with other changes, enhanced the functional properties of AP-PHL, including thermal stability, water solubility, and emulsification, thereby indicating its substantial potential for applications in food and pharmaceuticals.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Jiawang Huang,Jianing Shi,Jingmin Fu,Zhuolin Liu,Zhiying Feng,Kangyu Wang,Chunjing Chen,Yi Ning,Fangguo Lu,Ling Li
Ethnopharmacology relevance Maxing Shigan Decoction (MXSGD), derived from the traditional Chinese medicine (TCM) classic Shang Han Lun , is recognized as an effective TCM herbal formula for treating respiratory diseases, although its precise molecular mechanisms against influenza A virus (IAV) have not yet been fully elucidated. Aim of the study This study aimed to clarify the pharmacological effects of MXSGD on IAV-infected pneumonia mouse models, with a particular focus on its regulatory mechanism through miR-1260. Materials and methods The chemical composition of MXSGD was analyzed using an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). After establishing IAV-infected mouse models, the pathological changes in lung tissues were observed by hematoxylin-eosin (HE) staining; the pulmonary epithelial cells and macrophages in lung tissues were examined through immunological assays; and the expression levels of miR-1260 and its target genes in lung tissues were evaluated by RT-qPCR and Western blot analysis. A co-culture system of MLE-12 and RAW246.7 cells was constructed as the research model. Then, the intracellular miR-1260 expression was detected by RT-qPCR, and the Sema3A expression was examined by immunofluorescence. The macrophages were further transfected with miR-1260 mimic, inhibitor, and Sema3A siRNA to determine whether MXSGD exerts its therapeutic effects through the miR-1260-targeted Sema3A-mediated PI3K/AKT/mTOR signaling pathway. Subsequently, alveolar macrophage-depleted mouse models were established using CL2MBP, and were infected with IAV. To clarify the role of macrophages in the therapeutic mechanism of MXSGD against IAV, the body weight and organ indices of mice were monitored and recorded; the total protein content and levels of inflammatory factors in the bronchoalveolar lavage fluid (BALF) were measured; the histopathological changes were observed by HE staining; the pulmonary epithelial cells and macrophages in lung tissues were examined through immunological assays; and the expressions of miR-1260 and its target genes in lung tissues were evaluated by RT-qPCR and Western blot analysis. Results MXSGD was found to alleviate IAV-induced pathological injury and suppress macrophage recruitment in mouse lung tissues, while downregulating the viral load and levels of inflammatory factors. The attenuation of macrophage recruitment was closely associated with pulmonary epithelial cells, potentially mediated through the miR-1260-targeted Sema3A-regulated PI3K/AKT/mTOR signaling pathway. In cellular experiments, both cell types in the co-culture system were restored following MXSGD treatment, accompanied by lowered intracellular viral load and levels of inflammatory factors. Additionally, macrophage apoptosis and miR-1260 expression were significantly reduced, whereas the Sema3A fluorescence intensity in macrophages was markedly increased. By transfecting miR-1260 mimic, inhibitor, and Sema3A siRNA into co-cultured macrophages, the therapeutic effects of MXSGD were further confirmed to be associated with the miR-1260/Sema3A-mediated PI3K/AKT/mTOR pathway. In IAV-infected models established after pulmonary macrophage depletion, the therapeutic efficacy of MXSGD was partially weakened following elimination of alveolar macrophages. In general, above in vivo findings are consistent with the cellular experimental results, collectively supporting the involvement of the miR-1260-targeted Sema3A-mediated PI3K/AKT/mTOR signaling pathway in the therapeutic mechanism of MXSGD. Conclusions MXSGD was demonstrated to regulate the PI3K/AKT/mTOR pathway through the macrophage miR-1260/Sema3A axis, through which excessive inflammatory responses in IAV-induced pneumonia were effectively suppressed. The findings provide a novel therapeutic target for antiviral treatment in TCM.
研究领域:
免疫学
·
病毒学
·
分子生物学
·
呼吸病学
·
中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2013
·
IF 3.334
Guisheng Zhou,Hanqing Pang,Yuping Tang,Xin Yao,Yunhe Ding,Shaoqing Zhu,Sheng Guo,Dawei Qian,Juan Shen,Yefei Qian,Shulan Su,Li Zhang,Chun Jin,Yong Qin,Jin-Ao Duan
In this study, a rapid, simple and sensitive analytical method was developed for the quantitative determination of 20 nucleosides and nucleobases in functional foods at trace levels using hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HILIC-UPLC–TQ-MS/MS) in multiple-reaction monitoring (MRM) mode. Under optimised chromatographic conditions, good separation of 20 target compounds was achieved using a Waters Acquity UPLC BEH Amide column and gradient elution in 11 min. The limits of detection (LODs) and quantification (LOQs) were between 0.02–42.54 ng/mL and 0.05–98.18 ng/mL for the 20 analytes, respectively. This is the first report about simultaneous analysis of nucleosides and nucleobases in functional foods using this method, which afforded good linearity, precision, repeatability and accuracy. The method developed was successfully applied to quantify target compounds in batches of ginkgo seeds. The method potentially could be used to determine polar and trace-level nucleosides and nucleobases in ginkgo seeds.
研究领域:
分析化学
·
食品科学
✓ 已验证
DOI
高影响因子
Frontiers in Sustainable Food Systems
·
2024
·
IF 3.7
Tusneem Kausar,Salah Laaraj,Ashiq Hussain,Younes Noutfia,Mohamed Bouhrim,Ramzi A. Mothana,Omar M. Noman,Aymen Mubashar,Nida Firdous,Shafiqa Ali,Shazia Yaqub,Kaoutar Elfazazi
Introduction: Carrot (Daucus carota) is a nutrient-dense root vegetable, and carrot pomace is a by-product of the juice extraction procedure, which is recognized a source of phytochemicals and functional components. Almonds (Prunus dulcis) are packed with a decent amount of nutrients and bioactives.Methods: This study investigates the utilization of carrot pomace powder (CPP) and almond powder (AP) as partial substitutes for straight grade white flour (SGWF) in cakes to enhance their nutritional value and sensory appeal, while reducing waste from carrot processing. The aim is to assess the effects of incorporating CPP (at 5%, 10%, and 15%) and AP (at 5%) on the chemical composition and properties of cakes.Results and discussion: Comparative analysis reveals that AP is rich in fat (13.8 ± 0.6%) and protein (31 ± 0.6%), while CPP excels in ash (5.0 ± 0.5%) and fiber (43.3 ± 3.3%) content. Furthermore, SGWF exhibits the highest moisture content. Water holding capacities for CPP and AP are 5.2 ± 0.2% and 5.0±0.1%, respectively, and oil holding capacities are 1.3 ± 0.1% and 1.8 ± 0.2%, respectively. Additionally, CPP contains higher levels of sodium, potassium, and calcium, while AP contains higher levels of magnesium, iron, and zinc compared to SGWF. The addition of CPP and AP increases the ash, fat, fiber, and protein contents of the cakes. Notably, cakes containing 80% SGWF, 15% CPP, and 5% AP show significantly higher total phenolic content (TPC) (125.5 ± 3.8 mg GAE/100 g), total flavonoid content (TFC) (58.4 ± 1.4 mg QE/100 g), and DPPH activity (17.6 ± 2.7%) compared to control cakes. Furthermore, a significant increment in mineral content is observed in cakes with high levels of CPP and AP. Sensory evaluations reveal high acceptance of cakes containing 15% CPP and 5% AP. In conclusion, this study suggests that the incorporation of CPP and AP into cakes can improve their nutritional profile, antioxidant activity, and sensory characteristics, offering a sustainable approach to bakery product development.
研究领域:
食品科学
·
营养学
·
植物化学
✓ 已验证
DOI
高影响因子
Horticultural Plant Journal
·
2017
·Vol. 3
·期 4
·165-171
·
IF 0
Yanhui ZHANG,Peiying LIU,Qianqian KONG,Shiyan XING,Xiaojing LIU,Limin SUN
Flavonoids and terpene trilactones, especially, ginkgo flavonglycosides, ginkgolides and bilobalides in leaves of ginkgo trees, need to be studied for effective application of these active components with high medical and health-care values. This study was aimed to provide scientific bases for genealogies selection and harvest season confirmation for Ginkgo biloba . A high-performance liquid chromatographic method (HPLC-ELSD) was developed to determine the contents of terpene trilactone and flavonoid of 36 ancient G. biloba genealogies from 19 provinces in China. The study indicated that the content gradually increased from April to August, and thereafter declined. Analysis of variance indicated that the contents of terpene trilactone, flavonoids, and their respective components had significant difference among 36 genealogies. The cluster analysis showed that No. 72 (Xing'an, Guangxi), No. 58 (Youyang, Chongqing), No. 82 (Rugao, Jiangsu), No. 123 (Huixian, Gansu), No. 99 (Dujun, Guizhou), No. 10 (Tai'an, Shandong) and No. 133 (Mentougou, Beijing) genealogies have higher content of terpene trilactone and flavonoid. These results can help us to select superior variety containing high content of terpene trilactone and flavonoid.
研究领域:
植物化学
·
药用植物学
·
天然产物化学
·
林木遗传育种
·
药物代谢
✓ 已验证
DOI
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
·
2013
·
IF 2.823
Guihua Ruan,Zhengyi Chen,Meiping Wei,Yuhua Liu,Haiyun Li,Fuyou Du
Microwave-assisted enzymatic digestion (MAED) technique was applied for ginkgo protein digestion with both free and immobilized enzyme. Under the optimized conditions of MAED (0.01 g/mL substrate concentration of bromelain, 4500 U/g enzyme/ginkgo protein, 30 min, 300 W microwave power), a higher digestion rate (7.50%) and a significant increase in antioxidant activity (72.7 mg/g) were obtained in contrast with the conventional methods. With the optimized digestion conditions (0.625% glutaraldehyde (v/v), 0.4 mg/mL initial concentration of bromelain and 4 h of immobilization), the activity and effectiveness factor of immobilized bromelain were respectively 86 U and 81.6%. The results of ginkgo digestion by applying MAED indicated that the digestion rate of immobilized bromelain obtained by MAED method (6.41%) was comparable to that of free bromelain in the conventional digestion (8.13%). In both case with immobilized and free bromelain while applying MAED, a homogeneously abundant distribution of peptide fragments (from 7.863 Da to 5856 Da) and a few different peptide profiles were found. This report brings in conclusion that applying MAED with immobilized enzyme has the potential to obtain the highest number of antioxidant activity peptides.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Plant Science
·
2017
·
IF 4.291
Ni Jun,Hao Juan,Jiang Zhifang,Zhan Xiaori,Dong Lixiang,Yang Xiuli,Sun Zhehang,Xu Wenya,Wang Zhikun,Xu Maojun
The flavonoids in the extracts of Ginkgo leaves have been shown to have great medical value: thus, a method to increase the flavonoid contents in these extracts is of significant importance for human health. In the present study, we investigated the changes in flavonoid contents and the corresponding gene expression levels in post-harvest Ginkgo leaves after various treatments. We found that both ultraviolet-B and NaCl treatment induced flavonoid accumulation. However, gene expression analysis showed that the increases in flavonoid contents were achieved by different pathways. Furthermore, post-harvest Ginkgo leaves responded differently to NaCl treatment compared with naturally grown leaves in both flavonoid contents and corresponding gene expression. In addition, combined treatment with ultraviolet-B and NaCl did not further increase the flavonoid contents compared with ultraviolet-B or NaCl treatment alone. Our results indicate the existence of a novel mechanism in response to NaCl treatment in post-harvest Ginkgo leaves, and provide a technique to increase flavonoid content in the pharmaceutical industry.
研究领域:
植物学
·
药理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2025
·
IF 5.2
Jiawei Bao,Lu Li,Lanzhen Ban,Xia Pi,Chunmei Li
Bitter apricot kernel (BAK) is a good source of amygdalin and oil. However, the current production process faces challenges including the inability to simultaneously extract amygdalin and oil, and the risk of toxicity due to amygdalin contamination in the oil. A novel, green and efficient ultrasonication-assisted extraction (UAE) method based on a natural deep eutectic solvent (NADES) composed of choline chloride-lactic acid (CC-LA) was established for simultaneous extraction of amygdalin and oil from BAK for the first time. Under optimizing conditions of a liquid-to-solid ratio of 18 mL/g, ultrasonic power of 420 W, temperature of 42 °C, and extraction time of 32 min, the highest yields of amygdalin and oil were achieved at 10.22 ± 0.04 % and 45.64 ± 0.24 %, respectively. The yield of amygdalin in UAE based on CC-LA was twice and nineteen times that of methanol (90 %, v/v) and water, respectively. Meanwhile, the yield of oil obtained by this new method was comparable to that by organic solvent extraction methods. The fatty acid composition of oil obtained by this method was consistent with that by conventional extraction methods. Scanning electron microscopy (SEM) analysis indicated that CC-LA and UAE had a synergistic effect on the destruction of BAK’s microstructure, which facilitated the recovery of amygdalin and oil. Molecular dynamics simulation results showed that amygdalin in CC-LA reached a higher solvent-accessible surface area (SASA) and formed more hydrogen bonds with longer lifetimes and greater stability. Furthermore, this new method demonstrated potential for industrial scalability.
研究领域:
食品科学
·
植物化学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）