Synthetic and Systems Biotechnology
·
2024
·
IF 4.4
Bing Fu,Meng Chen,Xianfeng Bao,Jiajie Lu,Zhiwen Zhu,Fuyao Guan,Chuyang Yan,Peize Wang,Linglin Fu,Ping Yu
Vitamin B 2 is an essential water-soluble vitamin. For most prokaryotes, a bifunctional enzyme called FAD synthase catalyzes the successive conversion of riboflavin to FMN and FAD. In this study, the plasmid pNEW-AZ containing six key genes for the riboflavin synthesis was transformed into strain R2 with the deleted FMN riboswitch, yielding strain R5. The R5 strain could produce 540.23 ± 5.40 mg/L riboflavin, which was 10.61 % higher than the R4 strain containing plasmids pET-AE and pAC-Z harboring six key genes. To further enhance the production of riboflavin, homology matching and molecular docking were performed to identify key amino acid residues of FAD synthase. Nine point mutation sites were identified. By comparing riboflavin kinase activity, mutations of T203D and N210D, which respectively decreased by 29.90 % and 89.32 % compared to wild-type FAD synthase, were selected for CRISPR/Cas9 gene editing of the genome, generating engineered strains R203 and R210. pNEW-AZ was transformed into R203, generating R6. R6 produced 657.38 ± 47.48 mg/L riboflavin, a 21.69 % increase compared to R5. This study contributes to the high production of riboflavin in recombinant E. coli BL21.
研究领域:
生物工程
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2016
·
IF 3.902
Qi Ma,Jinping Song,Sufang Zhang,Meifang Wang,Yong Guo,Chuan Dong
β-Cyclodextrin-grafted citrate was used for the first time as a stabilizer and reducer to prepare silver nanoparticles (AgNPs). The as-synthesized AgNPs were further characterized by UV–vis absorption spectroscopy, powder X-ray diffraction spectroscopy, and transmission electron microscopy. The results show that the presence of riboflavin caused severe aggregation of the nanoparticles, thereby inducing a colour change from yellow to red. 1 H NMR further verified the formation of non-inclusion complexes between riboflavin and β-cyclodextrin-grafted citrate. Hydrogen bond was considered the main driving force of the interaction between the riboflavin and external rim of β-cyclodextrin. Based on these observations, the as-synthesized AgNPs were utilized to develop a novel colorimetric sensor for riboflavin detection. This colorimetric probe showed excellent selectivity and high sensitivity for riboflavin with a detection limit of 167 nM.
研究领域:
分析化学
·
纳米技术
·
光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Peng Zhang,Zhong-yu Bao,Hui Wang,Zong-cai Tu,Xiao-mei Sha,Yue-ming Hu
Soy protein isolate (SPI) was pre-treated with ultrasonic (400 W, 30–120 min) and then formed gel using transglutaminase (TG) method. Effects of ultrasonic pretreatment on structural, rheological and thermal properties as well as riboflavin delivery ability of SPI gel were investigated. Ultrasonic treatment significantly decreased the particle size, increased the free sulfhydryl content, enhanced the surface hydrophobicity, unfolded the conformational structure of SPI, facilitated the disulfide bonds and ε-(γ-glutamyl) lysine isopeptide bonds formation in transglutaminase-catalyzed soy protein isolate gel (TCSG). Ultrasonic pretreatment, particular for 60 min, significantly enhanced the gel strength, storage modulus, loss modulus, consistency and thermal stability, but decreased the flow behavior index of TCSG. The TCSG without ultrasonic pretreatment showed a non-uniform gel network with large and irregular pores. When applied ultrasonic pretreatment, more dense, uniform and stable gel networks were developed. The above physicochemical properties improvements inhibited the swelling behavior and markedly enhanced the riboflavin encapsulation efficiency and riboflavin delivery ability of TCSG, leading to 81% of riboflavin released in simulated intestinal environment. Therefore, ultrasonic pretreatment could be an efficient approach to improving the gel properties and nutrients delivery ability of SPI gel.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Bo Qiu,Huijuan Jing,Shengyi Han,Shuobo Zhang,Furong Ba,Lvwan Xu,Siyuan Xie,Wang Gao,Yilun Huang,Hang Xiao,David Julian McClements,Björn Berglund,Lanjuan Li,Mingfei Yao
Faecalibacterium prausnitzii ( F. prausnitzii ) exhibits a variety of biological functions that make it suitable for use as a next-generation probiotic . However, its high sensitivity to oxygen and digestive fluids currently limits its application. Riboflavin is known to support the growth of F. prausnitzii in oxygen environments, but it is important that it is in close proximity to the probiotics. Layer-by-layer assembly can be used to form protective coatings around probiotics, which can protect them from adverse environmental conditions. Moreover, riboflavin can be conjugated to these coatings, thereby increasing its efficacy by bringing it close to probiotic surfaces. In this study, we therefore evaluated the potential of electrostatic layer-by-layer assembly to protect F. prausnitzii by coating them with riboflavin-alginate and glycol-chitosan layers. Initially, we showed that riboflavin could be successfully conjugated to alginate, with a grafting ratio of around 4.35%. Then, the layer-by-layer method was used to coat F. prausnitzii using cationic glycol chitosan and anionic riboflavin-alginate. The coating formed was found to have a thickness of approximately 18.5 nm. Encapsulation did not adversely affect the growth of F. prausnitzii , but it significantly enhanced its resistance to oxygen and digestive fluids. The encapsulated probiotic was shown to have enhanced mucoadhesive properties using an in vitro intestinal monolayer model. Furthermore, the encapsulated probiotics colonized the colons of rats for longer than nonencapsulated ones. These results show that coating F. prausnitzii with riboflavin-rich biopolymer layers improves its resistance to oxygen and digestive fluids, and enhances its mucoadhesion and colonization properties, which should enhance its potential as an orally administered probiotic.
研究领域:
微生物学
·
药物递送
·
功能性食品
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Fazal Muhammad Khoso,Mei-Yi Duan,Bi Yang,Guo-Cong Liu,Xi Zhu,Jin-Gang Yu
Cadmium (Cd) pollution in water poses a serious environmental and health threat. GO composites exhibit significant potential as adsorbents for the removal of Cd2+ owing to their large surface area. However, their adsorption performances such as capacity, reusability, and selectivity can be further improved through rational functionalization. A novel RF-GO composite was fabricated through a facile covalent coupling method. The Cd2+ ion adsorption performance of the RF-GO composite was studied through batch adsorption experiments, and process parameter optimization including initial pH, temperature and contact time. The composite's structural morphology, surface functional groups, pore features, and thermal stability were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS), specific surface area analysis (SSA), and thermogravimetric analysis (TGA). The RF-GO composite exhibited a higher adsorption capacity of 39.58 mg g−1 for Cd2+ at pH = 8. The adsorption kinetics followed the pseudo-second-order kinetic model (R2 = 0.995), demonstrating a chemisorption-dominated process. Equilibrium data were best fitted with the Langmuir isotherm model (R2 > 0.97), suggesting the monolayer adsorption onto uniform active sites. Thermodynamic studies confirmed the endothermic and non-spontaneous nature of adsorption. Notably, the composite exhibited excellent reusability and structural stability, highlighting its potential as a sustainable and selective solution for Cd2+ removal from wastewater.
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Microbiology
·
2019
·
IF 4.259
Zou Long,Wu Xian,Huang Yunhong,Ni Haiyan,Long Zhong-er
The extracellular electron transfer (EET) that connects the intracellular metabolism of electroactive microorganisms to external electron donors/acceptors, is the foundation to develop diverse microbial electrochemical technologies. For a particular microbial electrochemical device, the surface chemical property of an employed electrode material plays a crucial role in the EET process owing to the direct and intimate biotic-abiotic interaction. The functional modification of an electrode surface with redox mediators has been proposed as an effectual approach to promote EET, but the underlying mechanism remains unclear. In this work, we investigated the enhancement of electrochemically polymerized riboflavin interface on the bidirectional EET of Shewanella putrefaciens CN32 for boosting bioelectrocatalytic ability. An optimal polyriboflavin functionalized carbon cloth electrode achieved about 4.3-fold output power density (∼707 mW/m2) in microbial fuel cells and 3.7-fold cathodic current density (∼0.78 A/m2) for fumarate reduction in three-electrode cells compared to the control, showing great increases in both outward and inward EET rates. Likewise, the improvement was observed for polyriboflavin-functionalized graphene electrodes. Through comparison between wild-type strain and outer-membrane cytochrome (MtrC/UndA) mutant, the significant improvements were suggested to be attributed to the fast interfacial electron exchange between the polyriboflavin interface with flexible electrochemical activity and good biocompatibility and the outer-membrane cytochromes of the Shewanella strain. This work not only provides an effective approach to boost microbial electrocatalysis for energy conversion, but also offers a new demonstration of broadening the applications of riboflavin-functionalized interface since the widespread contribution of riboflavin in various microbial EET pathways together with the facile electropolymerization approach.
研究领域:
微生物学
·
电化学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Jun Zhang,Xin Li,Jun-Cheng Zhang,Jia-Shu Yan,Haotian Zhu,Jiang-Jun Liu,Ru Li,Seeram Ramakrishna,Yun-Ze Long
Traditional indicator paper is disposable after use, requires a large number of target solutions and can only detect one item. In this study, recyclable upconversion-fluorescence indicator papers consist of upconversion nanorods and plasmonic nanostructures are fabricated by electrospinning. Human-body fluids containing different riboflavin concentration and pH are used as target solution, because these two items are usually detected in clinic to distinguish ambiguous diseases but often performed by two different testing processes that inevitably need more target solutions. Significantly different from conventional detecting system, this recyclable indicator paper can simultaneously achieve riboflavin and pH detections by using only a single droplet of target solution. As low as 0.01 ppm riboflavin can be sensitively detected, which is superior to most reported detection limit of fluorescence sensor. Moreover, this indicator paper can detect a single droplet (~10 μl) rather than a large amount of target solution (>1 ml) needed by traditional detecting system, and the pH can also be simultaneously in-situ detected using the same indicator paper. After the detection, the target solution is easily removed so that it brings about excellent recyclability, which is another advantage over traditional detecting system.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
NANOTECHNOLOGY
·
2022
·Vol. 33
·期 44
·445501
·
IF 3.953
Qi Yu,Jin Zou,Guanwei Peng,Feng Gao,Yansha Gao,Guorong Fan,Shangxing Chen,Limin Lu
Herein, a facile ratiometric electrochemical method was developed for sensitive sensing of riboflavin (RF) based on hierarchical porous biochar (HPB) modified electrode. In this sensing system, the reference paracetamol (PA) was directly added into electrolyte solution without the requirement of complex immobilization process. HPB derived from KOH-activated Soulangeana sepals displays hierarchical porous structure, high specific surface area and rich oxygen-containing functional groups, which is favorable for RF adsorption and enrichment. Besides, the excellent electronic conductivity and superior electrocatalytic activity of HPB can effectively promote the electrooxidation of RF. Moreover, the dual-signal strategy greatly improves the reproducibility and reliability of electrochemical detection. Based on the proposed ratiometric sensing platform, the sensor exhibits a wider linear range of 0.0007–10 μM and a lower limit of detection of 0.2 nM. The method also presents good selectivity and has been applied to the determination of RF in milk samples with satisfactory results.
研究领域:
分析化学
·
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Zhenghan Shi,Peixue Deng,Li-ang Zhou,Meng Jin,Feiyue Fang,Tao Chen,Guang Liu,Hao Wen,Zijian An,Hao Liang,Yanli Lu,Jun Liu,Qingjun Liu
Nutrition assessment is crucial for dietary guidance and prevention of malnutrition. Recent endeavors in wearable biochemical sensors have enabled real-time, in situ analysis of nutrients in sweat. However, the monitoring of riboflavin, an indispensable vitamin B involved in energy metabolism, remains challenging due to its trace level and variations in the sweat matrix. Herein, we report a wireless, battery-free, and flexible wearable biosensing system for the in situ monitoring of sweat riboflavin. Highly sensitive and selective electrochemical voltammetric detection is realized based on the synergistic effect of electrodeposited reduced graphene oxide (rGO) and platinum nanoparticles (PtNPs) with a low detection limit of 1.2 nM. The fully integrated system is capable of sweat sampling with the microfluidic patch, real-time riboflavin analysis and pH calibration with the flexible electrode array, as well as wirelessly simultaneous near field communication (NFC) energy harvesting and data transmission with the flexible circuit and a smartphone. On-body human sweat analysis demonstrates high accuracy cross-validated with gold-standard measurements, and reveals a strong correlation between sweat and urine riboflavin levels. The proposed wearable platform opens up attractive possibilities for noninvasive nutrient tracking, providing strong potential for personalized dietary guidance towards precision nutrition.
研究领域:
生物医学工程
·
营养学
·
传感器技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Hao-Tian Zhu,Mi Chen,Ai-Jun Wang,Pei-Xin Yuan,Jiu-Ju Feng
Nowadays, organic molecules with excellent fluorescence often lack electrochemiluminescence (ECL), which seriously restricts their analytical and biomedical applications. In this work, we functionalized phenazine (PZ) derivatives by with the charged moieties and activated their ECL properties using K 2 S 2 O 8 as a coreactant, achieving a significant “off-to-on” ECL emission transition. For that, 7,8-dihydroxyphenazine-2-carboxylic acid (DHPZ) was synthetized, showing the largest ECL efficiency of 56.4% (versus Ru(bpy) 3 2+ as a standard). Its subsequent self-assembly with ethylene glycol (termed as DHPZ-EG) as emitter further boosted the 4.0-fold ECL efficiency. In the presence of riboflavin (RF), the DHPZ-EG assemblies underwent the light-induced photocatalytic oxidation (PR), enabling efficient ECL signal transduction via the structure-property correlation. The as-established ECL sensor exhibited a wide linear section from 10 nM to 1.0 mM and a limit of detection (LOD, 1.5 nM), and the ability to monitor the broad bean growth metabolism. This work opens a novel avenue for designing organic luminophores and developing sensors for dynamic botanical tracking.
研究领域:
分析化学
·
生物传感
·
分子生物学
·
植物生理学
·
转化医学
·
光化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Hui He,Pir Muhammad,Zhanchen Guo,Qiling Peng,Haifeng Lu,Zhen Liu
Plasmon-enhanced fluorescence (PEF) is an emerging technology for sensitive detection . It relies on the plasmonic effect of a noble metal nanostructure to dramatically enhance the fluorescence of target fluorophores around the metal surface. Because there is a compromise between plasmonic enhancement and fluorescence quenching , it is critical to control the distance between the fluorophore and the metal surface to an appropriate range. This makes the fabrication of plasmonic nanostructures for PEF assays a challenging task. Herein, we report a controllably prepared core-shell plasmonic nanostructure coated with molecularly imprinted polymer (MIP) for sensitive and specific PEF assay. Riboflavin (RF) was used as a test compound in this study. RF-imprinted Ag@SiO 2 nanoparticles were prepared in a controllable manner, providing an optimal distance between the metal surface and RF molecules. The obtained hybrid nanostructure allowed for sensitive detection and specific recognition towards the target. Based on the plasmonic hybrid nanostructure, a sensitive and specific PEF assay of RF was developed and successfully applied to the determination of RF in human urine. Thus, the study paved the way for controllable preparation of molecularly imprinted plasmonic nanostructures for sensitive and specific PEF assays.
研究领域:
分析化学
·
纳米技术
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2017
·Vol. 135
·期 12
·46015
·
IF 1.86
Wen Yang,Xiaomin Wang,Shengnan Wang,Wentao Hao
The flexible, strong, and tough white-light-emitting (WLE) hybrid films are highly demanded in large-scale displays, including TV sets, monitors, and electronic interactive devices. In this work, a kind of WLE hybrid film was fabricated from hyperbranched poly(amido amine) (HPAMAM) and nanoclay with the incorporation of riboflavin (VB 2 ) and rhodamine B (RhB). The fluorescence emission of HPAMAM was partially absorbed and effectively transferred into green and red color, which combined into a bright white light with the residue blue fluorescence. Due to the alignment of nanoclay inside the HPAMAM matrix, in addition to the strong interaction among the HPAMAM molecules and that between HPAMAM and nanoclay, this hybrid film shows to be strong and tough. The mechanical strength is about 20 MPa and the elongation is about 30%. It is convinced that this hybrid film is promising in flexible large-scale displays. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135 , 46015.
研究领域:
材料科学
·
光学
·
高分子化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）