JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2018
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IF 3.412
Cun-Duo Tang,Hong-Ling Shi,Jian-He Xu,Zhu-Jin Jiao,Fei Liu,Peng-Ju Ding,Hong-Fei Shi,Lun-Guang Yao,Yun-Chao Kan
d-Mandelate dehydrogenase (DMDH) has the potential to convert d-mandelic acid to phenylglyoxylic acid (PGA), which is a key building block in the field of chemical synthesis and is widely used to synthesize pharmaceutical intermediates or food additives. A novel NAD+-dependent d-mandelate dehydrogenase was cloned from Lactobacillus harbinensi (LhDMDH) by genome mining and expressed in Escherichia coli BL21. After being purified to homogeneity, the oxidation activity of LhDMDH toward d-mandelic acid was approximately 1200 U·mg–1, which was close to four times the activity of the probe. Meanwhile, the kcat/Km value of LhDMDH was 28.80 S–1·mM–1, which was distinctly higher than the probe. By coculturing two E. coli strains expressing LhDMDH and LcLDH, we developed a system for the efficient synthesis of PGA, achieving a 60% theoretical yield and 99% purity without adding coenzyme or cosubstrate. Our data supports the implementation of a promising strategy for the chiral resolution of racemic mandelic acid and the biosynthesis of PGA.
研究领域:
生物化学
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生物技术
·
酶学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2021
·
IF 5.754
Zhang Jiyue,Gao Ningxuan,Shu Chi,Cheng Shunchang,Sun Xiyun,Liu Changjiang,Xin Guang,Li Bin,Tian Jinlong
The kiwi berry (Actinidia arguta) has been widely studied because of its rich phenolic, flavonoid, and vitamin C contents. Numerous reports have demonstrated that fruit peels contain higher phenolic content and antioxidant activity than that of flesh. In this study, the phytochemical content and antioxidant activities of peel and flesh extracts of six kiwi berries were analyzed from four regions (namely, Dandong, Benxi, Taian, and Tonghua) in China. The antioxidant activity was determined using the peroxyl radical scavenging capacity (PSC) and cellular antioxidant activity (CAA) assays. The phenolic, flavonoid, and vitamin C contents of kiwi berry peel were 10.77, 13.09, and 10.38 times richer than that of kiwi berry flesh, respectively. In addition, the PSC and CAA values of kiwi berry peel were higher than those of kiwi berry flesh. The analysis of the separation and contents of phenolics were performed by the high-performance liquid chromatography (HPLC)-diode-array detectormass spectrometry/mass (DAD-MS/MS) system, and the results illustrated that protocatechuic acid, caffeic acid, chlorogenic acid, and quinic acid were the major phenolic compounds. In conclusion, this study indicated that kiwi berry peel contains a rich source of antioxidants. These data are of great significance for the full development and utilization of kiwi berries in these four regions of China to produce nutraceutical and functional foods.
研究领域:
植物学
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营养学
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食品科学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2022
·
IF 7.968
Evans Dovi,Aaron Albert Aryee,Alexander Nti Kani,Farid Mzee Mpatani,Jianjun Li,Lingbo Qu,Runping Han
This research investigated the feasibility of removing hexavalent chromium (Cr(VI) (e.g., HCrO 4 − , CrO 4 2− and Cr 2 O 7 2− ions) from water using walnut shells functionalized with amino groups (ACWNS) in a batch and continuous adsorption mode. The characterization of ACWNS was carried out utilizing FTIR, XPS , SEM, and BET methods. Adsorption was investigated in terms of pH, dose, concentration, and contact duration. Furthermore, at different temperatures, the adsorption isotherms , kinetics, and thermodynamics were studied. The Langmuir model described the experimental batch and column modes, while the Thomas and pseudo-second-order kinetics models predicted continuous and batch adsorption, respectively. The maximum uptake quantity toward Cr(VI) was 236.8 mg g –1 in batch mode and 308.4 mg g –1 in column mode at 303 K. There is some selective adsorption for Cr (VI) with some dyes coexisting in solution. Electrostatic interactions and the reduction process mediated Cr(VI) adsorption by ACWNS. The mass transfer potential (B) and the affinity between ACWNS and Cr(VI) (β) were computed using the modified mass transfer factor model. Since the attraction of Cr (VI) to ACWNS is more robust, the B value increases as the concentration increases. Again, MTZ assessment reveals that as MTZ length rises, diffusion resistance increases, resulting in increased adsorption. The adsorbent demonstrated improved regeneration capacity and therefore showed potential for the uptake of Cr(VI) ions from wastewater. Availability of data and material The dataset produced and processed during this research is available upon reasonable request from the corresponding author.
研究领域:
环境科学
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化学工程
✓ 已验证
DOI
高影响因子
Sustainable Materials and Technologies
·
2021
·
IF 7.053
Zhengwei Wan,Yingchong Huang,Xiaomin Zeng,Xinzhu Guo,Zhuoying Wu,Miaomiao Tian,Gu Wu,Min Ling,Zeheng Li,Xuehui Gao,Chengdu Liang
The binder plays a crucial role in maintaining electrode structure and improving the cycling performance in Li S batteries , especially for high–energy–density batteries. Here, a natural biopolymer peach gum (PG) was developed as sulfur cathode binder for the first time. PG binder owns abundant carboxyls and hydroxyls, which can form strong interaction between Li O and further effectively constrain polysulfides from shuttling. Besides, strong adhesion force and superior mechanical behavior of PG help to keep the electrode integrity and good electrical contact. Therefore, PG@S electrode achieves an impressively high discharge areal capacity of 27.10 mAh cm −2 with sulfur loading of 26.3 mg cm −2 .
研究领域:
材料科学
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能源技术
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
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IF 6.6
Xiaoqin Wu,Zhiyi Shan,Lufan Wang,Wenwei Shi,Yanyan Li,Hongfang Cai,Liang Zhou,Chen Huan
Anthocyanins are bioactive substances that are not only responsible for the pigmentation of various fruits, but also hold potential benefits to human health. In this study, postharvest peaches were treated with 10 ppm 1-MCP, and changes of anthocyanin biosynthesis were then revealed at transcriptional level to gain insight into the underlying molecular mechanisms. The functions of key transcription factors (TFs) were systematically analyzed. Results demonstrated that 1-MCP effectively up-regulated expression of structural genes and stimulated anthocyanin accumulation. A novel transcription factor PpbHLH135 can respond positively to 1-MCP treatment and has been noted for its remarkable gene expression and protein abundance. The transcription factor bHLH3 was also investigated in this study due to its widespread interest and attention, serving to compare the regulatory function of bHLH135 in anthocyanin synthesis, although it didn’t show a significant response to 1-MCP treatment in this study. PpbHLH3 TF showed a stronger regulatory effect on PpF3H , while PpbHLH135 was more dominant over the regulation of PpUFGT . Future genetic and molecular experimentation will elucidate whether PpbHLH135 TF, in combination with other TFs, can play a stronger regulating role in fruit pigmentation, or in other metabolic pathways.
研究领域:
植物学
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ximei Han,Jiayi Chen,Qian Wang,Jie Zhang,Jiaqi Mi,Jianxing Feng,Ting Du,Jianlong Wang,Wentao Zhang
Natural photosensitizers offer promising and sustainable solutions to the challenges of food preservation. This study investigates the potential of chlorogenic acid (CA), a naturally occurring phenolic compound with dual photoactive and antimicrobial properties. Under Xenon lamp irradiation (100 mW/cm 2 ), CA at a concentration of 0.5 mg/mL demonstrated significant antimicrobial efficacy against both Staphylococcus aureus (10 6 CFU/mL) and Escherichia coli (10 5 CFU/mL). To enhance the practical applicability of CA for cherry preservation, CA was incorporated into agar (AG) films, which exhibited superior physicochemical and mechanical properties, including increased tensile strength and improved gas permeability. Implementation of CA-AG films prolonged the storage duration of cherries by 9 days through effective quality retention and suppression of microbial contamination. This research highlights the potential of CA as an environmentally friendly and functional solution for advanced food preservation technologies.
研究领域:
食品科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Lei Yao,Chao Hong,Yangxu Qi,Lie Wu
In this study, we developed a novel magnetic composite of walnut shell biochar (WSB) and modified it with ZIF-7 for the purpose of removing Co 2+ ions. We conducted a comprehensive evaluation of its performance in Co 2+ removal, considering various factors such as contact time, temperature, adsorbent dose, pH, and initial Co 2+ concentration. Utilizing response surface methodology-central composite design (RSM-CCD), we examined the interactions between these variables. The specific surface area and magnetic saturation value of the WSB/MnFe 2 O 4 /ZIF-7 composite were determined as 170.560 m 2 .g −1 and 23.469 emu.g −1 , respectively. ANOVA analysis indicated that our model effectively captures the interactions between variables, with a high R 2 value of 0.9982, explaining 99.82 % of the experimental data. Optimal conditions for maximum Co 2+ adsorption efficiency (99.05 %) were identified as follows: a contact time of 44.82 min, pH of 6.04, adsorbent dose of 0.947 mg.L -1 , temperature of 25.55 °C, and Co 2+ concentration of 11.13 mg.g −1 . Kinetic studies revealed that the Pseudo 2nd order kinetic model provided the best fit to the experimental data. The negative entropy parameter indicated a decrease in disorderliness during the adsorption process, while the exothermic nature of adsorption was confirmed by enthalpy values of −92.751 and −40.511 kJ.mol −1 for WSB/MnFe 2 O 4 /ZIF-7 and WSB, respectively. Furthermore, the Langmuir isotherm model demonstrated excellent fitting to the isotherm results, suggesting the dominance of homogeneous surfaces in adsorption. The maximum adsorption capacities for Co 2+ using WSB/MnFe 2 O 4 /ZIF-7 and WSB were determined to be 29.26 and 22.39 mg.g −1 , respectively. The WSB/MnFe 2 O 4 /ZIF-7 magnetic composite demonstrates promising potential for efficiently removing Co 2+ from both natural water sources and industrial wastewater, highlighting its versatility and effectiveness in environmental remediation applications.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
POLYMER BULLETIN
·
2025
·
IF 4
Gorar Athar Ali Khan,Zhiyi Guo,Zhicheng Wang,Daham Abbas,wang Xudong,Wang Jun,Liu Wen-Bin
Using agricultural waste as biocarbon material is a recent development with many potential applications. Nutshells can be utilized to produce biocarbon, enhancing the electrical properties of thermoset composites. In this research, Walnut shells (WSs) were carbonized at 750 °C, and activated carbon filler was thoroughly examined. Isothermal compression molding was used, and composites were produced by incorporating 1–2.5 wt.% of carbonized bio-filler (CW) into Vanillin-furfuryl amine-derived bio-benzoxazine. The composites showed improved thermal stability, increasing from 293 to 321 °C; Char yield enhanced from 53.3% to 60.4%. The flexural modulus and flexural strength exhibited enhancements of 41.3% and 20%, respectively, while toughness significantly increased from 0.87 to 0.94 N-mm compared to the neat matrix. Heat deflection temperature tests showed improved thermomechanical properties in composites. Flammability tests indicated that they are flame-resistant and fire-safe due to the CW filler. Thermal conductivity enhanced by 6%, and dielectric characteristics increased by 56.7% with 2.5 wt.% filler reinforcement. With the above findings, these composites can be utilized in designing parts exposed to fire, high temperatures, efficient energy storage, enhanced functionality in electronic devices, and improved insulation efficiency.
研究领域:
材料科学
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可持续发展
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
ACTA AGRICULTURAE SCANDINAVICA SECTION B-SOIL AND PLANT SCIENCE
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2018
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IF 0.894
Yujie Yin,Pei’en Ni,Bohan Deng,Shiping Wang,Wenping Xu,Dapeng Wang
Purpose: Pseudomonas syringae pv. actinidiae causes bacterial canker of kiwifruit and is responsible for severe economic losses and emergence of drug-resistant bacteria. Bacteriophages are viruses that infect target bacterial hosts and may be the best strategy to prevent and control kiwifruit canker disease. The objective of this experiment was to monitor the prevalence of Pseudomonas syringae pv. actinidiae and provide insight for the use of phages in biological control.Materials and methods: In this study, 52 strains of Pseudomonas syringae pv. actinidiae were isolated from 68 stem samples of kiwi plant (cv. Hongyang & Jinkui). Following polymerase chain reaction (PCR) analysis, 15 isolates belonging to biovar 3 were identified, one of which was named XWY0007 and used as the target strain to isolate the phages. Thirty-six phages were isolated and purified from a total of 51 surface water samples collected in Shanghai. All phages were identified by transmission electron microscopy (TEM) and their host ranges were evaluated. Three phages, designated φXWY0013, φXWY0014 and φXWY0026 were selected and further characterised using one-step growth curve and stability at different temperatures and pH.Results and conclusions: The isolated phages are promising for use as antimicrobials against bacterial canker in kiwi. This report is regarding Pseudomonas syringae pv. actinidiae and its phages from major areas of kiwifruit cultivation.
研究领域:
植物病理学
·
微生物学
✓ 已验证
DOI
高影响因子
WASTE MANAGEMENT
·
2021
·
IF 7.145
Xiefei Zhu,Zejun Luo,Xifeng Zhu
Enriching high-value chemicals from the pyrolysis of agricultural and forestry waste is an efficient way to achieve sustainable development and large-scale application of biomass pyrolysis. Phenols, as important chemical raw materials, spices and food additives, have attracted widespread attention. Herein, a novel technical route of torrefaction pretreatment combined with fractional condensation in pyrolysis loop was proposed to enrich the phenols in liquid products. In this study, the enrichment of phenols from the pyrolysis loop of walnut shell under the combination of torrefaction and fractional condensation was explored using a fixed-bed pyrolysis reactor equipped with a three-stage condensation system. Simultaneously, the effects of torrefaction on feedstocks were investigated through a thermogravimetric analyzer based on the characteristics of feedstocks. The results showed that the torrefaction and pyrolysis loop had a negative impact on the pyrolysis efficiency and the yield of liquid products, while the change in the condensation efficiency depended on the combined effects of torrefaction and pyrolysis loop. In addition, phenols tended to be enriched in the second condensation stage, especially phenol, o-cresol, 4-ethylphenol. Importantly, torrefaction could significantly enrich phenols in the liquid products, and the enrichment of phenols is relatively increased by 109.44% at least. Moreover, the pyrolysis loop was also beneficial to the enrichment of phenols, which was at least 90% higher than that of walnut shell. This study provided a potential route to enrich high value-added products from the pyrolysis loop of lignocellulosic biomass.
研究领域:
生物质能
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化学工程
·
可持续发展
✓ 已验证
DOI
高影响因子
JOURNAL OF THERMOPLASTIC COMPOSITE MATERIALS
·
2019
·
IF 1.343
Hongjuan Zheng,Zhengqian Sun,Hongjuan Zhang
Poly(lactic acid) (PLA) has good environmental compatibility, however, its high brittleness, slow rate of crystallization, and low heat distortion temperature restrict its widespread use. To overcome these limitations, in this study, PLA was mixed with walnut shell (WS) powders. The effects of WS powders on the morphology and the thermal and mechanical properties of PLA were investigated. The products were characterized by differential scanning calorimetry (DSC), infrared (IR) spectroscopy, polarizing optical microscopy (POM), and various mechanical property testing techniques. The results showed that WS powders had a significant effect on the morphology and the thermal and mechanical properties of PLA. The tensile strength, impact strength, and elongation at break of the PLA/WS composites first increased and then decreased with the increasing addition of WS powders. When the addition of WS powders was about 0.5 wt%, they reached maximum values of 51.2 MPa, 23.3 MPa, and 19.0%, respectively. Compared with neat PLA, the spherulite grain size of the composites could be reduced and many irregular polygons were formed during crystallization. The melting, cold crystallization, and glass-transition temperatures of the composites were lower than those of neat PLA.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Food Measurement and Characterization
·
2021
·Vol. 15
·期 3
·2716-2726
·
IF 2.431
Qian Junqing,Zhao Changyan,Zhu Hanxiao,Tong Jun,Zhao Xiaohua,Yang Haiyan,Guo Hui
Walnut oil is endowed with a variety of physiological functions due to abundant natural compounds, and the effect of unsaturated fatty acids is crucial. In order to accurately and conveniently detect the fatty acids components and content in walnut oil, a quantitative method was developed to detect fatty acids content based on the 1 H-NMR and 13 C-NMR techniques. In addition, HPLC combined with MS was used to conduct qualitative analysis. HPLC method with an evaporative light-scattering detector was for the quantitative analysis and the fatty acids content was compared with the content from NMR. The result showed that there was no significant difference between 1 H-NMR, 13 C-NMR and HPLC (P > 0.05) and indicated that the NMR has the potential to be applied as a routine method for the analysis of fatty acids content in walnut oil.
研究领域:
分析化学
·
食品科学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）