JOURNAL OF APPLIED TOXICOLOGY
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2024
·
IF 2.7
Canyang Wang,Zhengzheng Zhou,Yayu He,Juan Li,Yi Cao
Food contaminates, such as insecticide, may influence the toxicity of nanoparticles (NPs) to intestine. The present study investigated the combined toxicity of TiO 2 NPs and fipronil to male mouse intestine. Juvenile mice (8 weeks) were orally exposed to 5.74 mg/kg TiO 2 NPs, 2.5 mg/kg fipronil, or both, once a day, for 5 days. We found that both TiO 2 NPs and fipronil induced some pathological changes in intestines, accompanying with defective autophagy, but these effects were not obviously enhanced after TiO 2 NP and fipronil co-exposure. Fipronil promoted Ti accumulation but induced minimal impact on other trace elements in TiO 2 NP-exposed intestines. Metabolomics data revealed that the exposure altered metabolite profiles in mouse intestines, and two KEGG pathways, namely, ascorbate and aldarate metabolism (mmu00053) and glutathione metabolism (mmu00480), were only statistically significantly changed after TiO 2 NP and fipronil co-exposure. Five metabolites, including 2-deoxy-D-erythro-pentofuranose 5-phosphate, 5alpha-cholestanol, beta-D-glucopyranuronic acid, elaidic acid, and isopentadecanoic acid, and maltotriose, were more significantly up-regulated after the co-exposure, whereas trisaccharide and xylonolactone were only significantly down-regulated by the co-exposure. We concluded that fipronil had minimal impact to enhance the toxicity of TiO 2 NPs to mouse intestines but altered metabolite profiles.
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高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Zhengtao Li,Haowei Dong,Haifang Wang,Donghan Li,Shuxian Zhou,Shengxi Zhai,Jingcheng Huang,Rui Xu,Wenping Zhao,Mohamed Bedair M. Ahmed,Gili Bisker,Xia Sun,Yemin Guo
The presence of multiple pesticide residues in vegetables has become an increasingly critical concern. Immunochromatography has garnered significant interest for its convenience, sensitivity and speed. However, conventional immunochromatography was typically limited to single-target detection, exhibiting restricted efficiency and high costs in on-site large-scale detection scenarios. Dual-channel time-resolved fluorescent immunochromatography test strip (TRFITS) was developed for the simultaneous rapid detection of imidacloprid (IMI) and tolfenpyrad (TOL) in 15 min. Utilizing the excellent optical properties of time-resolved fluorescent microspheres, this TRFITS enabled better detection sensitivity (0.22 ng/mL for IMI, 11.85 ng/mL for TOL), which was approximately 20 times more sensitive than that of conventional immunochromatography, under optimal conditions. In addition, the TRFITS successfully detected IMI and TOL in vegetables samples with acceptable accuracy. The ability to simultaneously quantify multiple pesticide residues using a single test strip reduces material consumption and lowers operational costs, offering a practical solution for rapid on-site pesticide screening.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-11
·
IF 5.3
Chen Yunhai,Zhu Xuecheng,Liu Huilin,Sun Baoguo
Room temperature phosphorescent carbon dots ( NC CDs@SiO 2 ) were obtained by encapsulating hydrothermally synthesized CDs in a dense Si-O network structure after high-temperature calcination using silica as the matrix. This can avoid the quenching effect of dissolved oxygen in water and has a phosphorescence lifetime of up to 2.41 s. Using the phosphorescence property of NC CDs@SiO 2 , a phosphorescence quenching sensor was developed for the sensitive and selective detection of thiram with the assistance of Cu 2+ . Cu 2+ -thiram complexes led to a rapid phosphorescence quenching of NC CDs@SiO 2 within 30 s through the inner filter effect. The linear range of phosphorescence for thiram was 0.5–100 µM with a detection limit of 0.121 µM. The proposed method was able to detect thiram in real samples and was validated by high-performance liquid chromatography (HPLC) confirming the potential of this phosphorescence sensing method for thiram detection. This work opens up a new avenue for the detection of thiram residues in fruits and vegetables and also provides a new idea for the design of a rapid detection platform using other room temperature phosphorescent materials. Graphical Abstract
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2018
·
IF 3.44
Xinda Lin,Yiwen Mao,Ling Zhang
Insects receive a variety of chemical signals from the environment. Chemosensory protein (CSP) is one of the olfactory proteins that can accommodate a variety of small molecules and have the ability to bind to lipophilic compounds, transmitting nonvolatile odor molecules and chemical stimuli to target cells. To understand the correlation between the insect olfactory system and environment, we identified four antennae-expressed SlituCSP genes and investigated their expression profiles after treatment with different temperatures, starvation and three commonly used pesticides : chlorpyrifos , emamectin benzoate and fipronil . The transcriptions of four SlituCSP genes are affected by pesticide treatment and less affected by starvation and different temperatures. To further understand the molecular function of CSPs and their correlation with pesticides, we expressed and purified four SlituCSPs and assayed their binding ability with pesticides. The binding of four SlituCSPs with three pesticides were determined using a fluorescence competitive binding assay. We found direct binding between CSPs and pesticides, especially between SlituCSP18 and chlorpyrifos/fipronil and between SlituCSP6 and all three pesticides. The high binding affinity with pesticides and the significant down-regulation of SlituCSP18 by chlorpyrifos suggests that SlituCSP18 is more sensitive to pesticide treatment and may play an important role in mediating the interaction of the olfactory system and the pesticide. This study can help us understand the role of CSP proteins in the adaption of S. litura to the environment.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Hong Sun,Xinyu Li,Xinyue Yuan,Zhen Tian,Yifan Li,Yalin Zhang,Jiyuan Liu
As an important class of detoxifying enzymes , glutathione S-transferases (GSTs) are pivotal in decreasing insecticide toxicity to insects. Periplaneta americana GSTd1 (PaGSTd1) has been verified as a key enzyme in detoxifying pyrethroid insecticides, but its detoxification capability against a broader spectrum of insecticides has never been investigated. It is revealed that PaGSTd1 expression showed a rapid and significant increase upon exposure to various insecticides (organophosphates, neonicotinoids , and fipronil). Subsequent in vitro metabolic assays indicated that organophosphates , particularly chlorpyrifos-methyl, can be effectively metabolized by PaGSTd1. Further knockdown of PaGSTd1 via RNA interference significantly heightened the susceptibility of P. americana to chlorpyrifos-methyl, underscoring the enzyme's key role in detoxifying chlorpyrifos-methyl. Additionally, this study confirmed that PaGSTd1 cannot mitigate insecticide toxicity through countering oxidative stress . Collectively, these findings elucidate the involvement of PaGSTd1 in the detoxification processes for organophosphates, offering a comprehensive insight into the metabolic mechanisms mediated by GSTs in P. americana . This research provides a foundational understanding for managing GSTs-mediated metabolic resistance in this species, which is crucial for effective pest control strategies.
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Journal of Inorganic and Organometallic Polymers and Materials
·
2024
·
IF 3.9
Pei Xiu,Bai Jinchuan,Li Yaming,Kong Xiangbo
In this paper, a luminescent metal-organic framework Tb 3+ @tartaric acid-functionalized Zr-MOF (Tb 3+ @UiO-67-bpy-TA, TA = tartaric acid) was prepared by post-synthetic modification (PSM) and used as a fluorescence sensor for the detection of the pesticide nitenpyram (NTP). The fluorescence sensing experiment verified that Tb 3+ @UiO-67-bpy-TA had exceptional sensitivity and selectivity for NTP detection, and the detection limit was calculated to be as low as 1.28 µM. The mechanism studies indicated that competitive UV absorption, photoinduced electron transfer (PET) and dynamic quenching contributed to the fluorescence quenching process. In addition, the detection of NTP in tap water and Yellow River water samples by the Tb 3+ @UiO-67-bpy-TA were also investigated using standard addition method, and satisfactory recoveries were achieved.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Linghui Huang,Yue Tang,Jianxun Han,Xiaojuan Niu,Xin Lin,Yuangen Wu
Malathion causes a serious threat to human health due to its widespread use in the environment. Herein, a novel and stable smartphone-integrated colorimetric biosensor for malathion detection is firstly established based on aptamer-enhanced laccase-mimicking activity. The results indicate that the M17-F aptamer can increase the affinity of Ag 2 O nanoparticles to the substrate 2,4-dichlorophenol and enhance their laccase-mimicking activity. Thus, abundant semiquinone radicals are produced in the catalytic system, which are combined with chromogenic agent to generate dark red products. The corresponding RGB values for the colour change of the solution can be easily obtained using smartphones, which is used for the rapid detection of malathion. The established biosensor for malathion has a limit of detection as low as 5.85 nmol·L −1 , and displays good selectivity for other competitive pesticides. Moreover, further studies have verified the applicability of the biosensor in actual samples, indicating that it may have the potential for application in malathion detection in food.
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ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Zhangchi Xiao,Yong Li,Yulun Nie,Liqiang Lu,Chao Yang
Background Methyl parathion, a potent organophosphorus insecticide, is extensively employed in agriculture and animal husbandry, leading to significant environmental contamination with pesticide residues, posing a grave threat to human health. This compound irreversibly inhibits acetylcholinesterase (AChE) in the human nervous system, resulting in the accumulation of acetylcholine (ACh), which is detrimental. Various enzyme activity-based assays have been explored due to its pathogenic mechanism, yet these methods are fraught with limitations. Thus, the development of a highly selective, sensitive, rapid, and effective method for detecting methyl parathion residues is crucial. Result In this study, we introduce a novel, non-enzymatic fluorescent probe for methyl parathion detection. This probe is designed to specifically trigger the fluorescence signal of TAPB-DMTA-COF through the interaction of copper ions with the imine moiety within the TAPB-DMTA-COF structure. The detection of methyl parathion in aqueous environments is facilitated by the Inner filter effect. Compared to previously reported enzyme-catalyzed reaction probes, this probe exhibits a faster reaction time, is not influenced by enzyme activity, and demonstrates high specificity and sensitivity. The probe exhibits a good linear response in the concentration range of 0.56–5.46 μM, with a detection limit of 30.698 nM. Furthermore, the probe yielded satisfactory recoveries of methyl parathion (93.47 %–99.5 %) in different real water samples through spiking recovery experiments. Significance The fluorescent probe based on Cu 2+ /TAPB-DMTA-COF realizes the fluorescence detection of methyl parathion. The establishment of this simple, efficient, and rapid detection platform offers a new approach for the detection of pesticide residues in aquatic environments. The introduction of metal ions, along with the construction of COF materials and the use of fluorescence signal turn-on, provides a rational design strategy for such detection methods.
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高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ziyi Qin,Jinju Xu,Yuanxin Cao,Chunhui Liao,Tian Lan,Shuyun Shi
Highly sensitive monitoring of pesticides has garnered attention for food safety. Here, a ratiometric fluorescent probe (AG-CDs-Al 3+ /CdTe QDs) was fabricated for specific and sensitive sensing of glyphosate. Surface modifications revealed the chelation-enhanced fluorescence process between the surface -OH of AG-CDs and Al 3+ . Positively charged green-emission AG-CDs-Al 3+ assembled with negatively charged red-emission CdTe QDs, and photoinduced electron transfer (PET) occurred from CdTe QDs to AG CDs-Al 3+ . Glyphosate specifically coordinated with Al 3+ , causing remarkable variations in F 500 /F 630 (linear range, 0.2–4.0 μM; detection limit, 5.7 nM) and a distinguishable green-yellow-red color change on the paper platform (linear range, 0.5–8.0 μM; detection limit, 26 nM). Furthermore, for real sample analysis, the sensor has the advantages of fast response (5 min), excellent accuracy (spiked recoveries, 95.0–105.0 %), high precision (relative standard deviation, < 3.33 %), and superior specificity, which endowed the designed sensing platform with great potential practicality in the field of food analysis.
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高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yehong Han,Ruiyang Ma,Juntao Wang,Hongyuan Yan,Yufei Shan,Zihan Liu,Zhi Wang
In this study, for the effective determination of fipronil and ethiprole residues in milk, a new analytical approach, which was based on a tip solid-phase extraction (TSPE) by using a newly prepared ternary deep eutectic solvent-functionalized UiO-66 (TDES@UiO-66) as the adsorbent and HPLC-UV detection, was developed. The method integrated the advantages of both the high adsorption capability of the TDES@UiO-66 and the high efficiency of the TSPE, and enabled a rapid extraction of fipronil and ethiprole from milk samples within 8 min by utilizing only 3.0 mg of TDES@UiO-66 adsorbent. The developed method demonstrated an excellent analytical performance, including good linearity (linear rang: 0.02–10 μg/mL; r : 0.9990–0.9997), high precision (relative standard deviations: ≤ 9.9 %), good accuracy (recoveries: 91.1 %–109.8 %), and good sensitivity (limits of quantification: 0.011–0.018 μg/mL). This study provides a rapid and practical approach for the detection of fipronil and ethiprole in different milk samples.
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高影响因子
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