Marine Life Science & Technology
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2025
·
IF 5.3
Wang Yan,Wang Zhen,Zhao Wen-Xiao,Yuan Xiao-Jie,Yu Yang,Wang Peng,Wang Min,McMinn Andrew,Zhang Yu-Zhong,Peng Ming,Fu Hui-Hui,Chen Xiu-Lan
Free trans -4-hydroxy-L-proline (T4LHyp), a non-proteinogenic amino acid, is mainly released from the degradation of collagen, hydroxyproline-rich glycoproteins, and some peptide antibiotics in nature. Although it has been known that some terrestrial bacteria utilize T4LHyp as carbon and nitrogen source via a T4LHyp gene cluster, which and how marine microorganisms catabolize T4LHyp still remains unclear. Here, five T4LHyp-utilizing marine bacterial strains, Halomonas sp. 5021, Salinicola sp. 4072, Alteromonas sp. 6022, Alteromonas sp. 5112 and Alteromonas sp. 30521, were isolated from hydrothermal vent sediment samples collected from the southwest Indian Ocean. While Halomonas sp. 5021 can utilize T4LHyp as both a nitrogen and carbon source, the other four strains can utilize T4LHyp as only a nitrogen source. Then, the T4LHyp catabolic mechanisms of Halomonas sp. 5021 and Salinicola sp. 4072, as a representative of the four strains, were further investigated by genomic, transcriptional, and biochemical analyses. Halomonas sp. 5021 adopts an intact T4LHyp gene cluster containing four enzymes to catabolize T4LHyp into NH 3 and α-ketoglutarate to provide nitrogen and carbon sources for its growth. Compared to Halomonas sp. 5021, Salinicola sp. 4072 lacks an α-KGSA dehydrogenase gene in the T4LHyp gene cluster and can only catabolize T4LHyp into NH 3 and α-ketoglutarate semialdehyde to provide a nitrogen source for its growth. Bioinformatic investigation showed that the 5021-like and 4072-like T4LHyp gene clusters are predominantly found in bacteria from Pseudomonadota , which are widely distributed in multiple marine habitats. Thus, Pseudomonadota bacteria are likely the dominant group to drive the recycling and mineralization of T4LHyp in the ocean.
研究领域:
海洋微生物学
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微生物生态学
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生物化学
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基因组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2016
·
IF 4.165
Yang Jie,Wang Guozeng,Ng Tzi Bun,Lin Juan,Ye Xiuyun
Laccases can oxidize a wide range of aromatic compounds and are industrially valuable. Laccases often exist in gene families and may differ from each other in expression and function. Quantitative real-time polymerase chain reaction (qPCR) was used for transcription profiling of eight laccase genes in Cerrena sp. strain HYB07 with validated reference genes. A high laccase activity of 280.0 U/mL was obtained after submerged fermentation for 5 days. Laccase production and laccase gene transcription at different fermentation stages and in response to various environmental cues were revealed. HYB07 laccase activity correlated with transcription levels of its predominantly expressed laccase gene, Lac7. Cu2+ ions were indispensable for efficient laccase production by HYB07, mainly through Lac7 transcription induction, and no aromatic compounds were needed. HYB07 laccase synthesis and biomass accumulation were highest with non-limiting carbon and nitrogen. Glycerol and inorganic nitrogen sources adversely impacted Lac7 transcription, laccase yields, and fungal growth. The present study would further our understanding of transcription regulation of laccase genes, which may in turn facilitate laccase production as well as elucidation of their physiological roles.
研究领域:
分子生物学
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微生物学
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生物技术
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环境科学
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产品关联
DOI
PubMed
高影响因子
Journal of Central South University
·
2023
·Vol. 30
·期 10
·3248-3259
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IF 4.4
Zhang Wen,Liu Ye,Ye Zhong-qiang,Yang Min,Luo Qi,Dai Jing,Wang Qian-fu,Liu Li
Potassium-ion batteries (PIBs) are regarded as one of the alternatives to lithium-ion batteries due to the vast potassium reserves in nature and low potential of K + /K. Therefore, in this work, we successfully synthesized porous nitrogen-doped FeP/C nanofibers (P-FeP/C-N NFs), a novel electrode material for PIBs, via simple electrospinning and subsequent calcination treatment, in which polyacrylonitrile (PAN) and polymethylmethacrylate (PMMA) are used as nitrogen source and pore-forming agent, respectively. The introduction of nitrogen improves the conductivity of the material, and the formation of a porous structure increases the specific surface area. More importantly, when the P-FeP/C-N NFs were used as electrode for potassium ion batteries, they exhibit excellent electrochemical performance. The P-FeP/C-N NFs electrode exhibits a discharge capacity of 274.2 mA·h/g at 100 mA/g after 250 cycles and 171.3 mA·h/g at 1000 mA/g after 300 cycles. Even at 5000 mA/g, it displays a high discharge capacity of 150.1 mA·h/g. These findings indicate that the P-FeP/C-N NFs are expected to be a candidate of anode materials for potassium ion batteries.
研究领域:
材料科学
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电化学
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能源存储
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
·
IF 4.952
Xi Cao,Yayu Pan,Wei Wei,Tinglan Yuan,Sen Wang,Lingyun Xiang,Yongjun Yuan
Effects of various fermentation conditions on fatty acid composition of single cell oil (SCO) from Trichosporon sp. F1-2 were investigated. Ten carbon sources clustered into two groups using hierarchical clustering algorithm and principal component analysis. Xylose and soluble starch from each group were further studied. SCO using xylose had a high oleic acid content, while the linoleic acid and α-linolenic acid content increased significantly in SCO using soluble starch especially at the sn -2 position. 20 and 31 triacylglycerol (TAG) types were detected, respectively. The increased types were rich in linoleic acid and α-linolenic acid. The effect of C/N ratios was the weakest (distance threshold 3.05), followed by inoculation amounts (3.08), fermentation time (4.30) and nitrogen sources (5.64). The optimum nitrogen source, C/N ratio, inoculation amount and fermentation time for SCO accumulation were ammonium sulfate, 60, 6% and 120 h, respectively. Two different SCOs were produced using xylose and soluble starch under optimum conditions, which were demonstrated to be potential substrates for synthesis of 1,3-distearoyl-2-oleoyl-glycerol (a cocoa butter improver) and MLM-type TAG (the dietary TAG with low caloric value) respectively. Therefore, changing the carbon source can help broaden application fields of SCOs and increase feedstock sources of structured TAG synthesis.
研究领域:
生物技术
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食品科学
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微生物学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）