APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2024
·Vol. 196
·期 9
·6378-6394
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IF 3.1
Wang Xiaoyu,Cong Qianming,Feng Chenxu,Sun Zizhan,Cai Zhengyu,Fan Chuangang,Pei Lizhai
Terbium vanadate nanowires were synthesized via a facile chemical approach using sodium vanadate and terbium chloride. Morphology, structure, composition, and electrochemical characteristics of the terbium vanadate nanowires were investigated by different techniques. Terbium vanadate nanowires with single crystalline tetragonal TbVO 4 phase possess smooth surface and flat tips. The length of the nanowires is longer than 5 μm, and diameter is 40–100 nm. Terbium vanadate nanowires modified electrode was used for trace-level mercury ions (Hg 2+ ) detection. One well-defined stripping peak exists at − 0.34 V at the terbium vanadate nanowires modified electrode in 0.1 mM Hg 2+ solution. Buffer solution pH value, deposition time, deposition potential, and standing time are pH = 1, 150 s, − 1.5 V, and 60 s, respectively. Detection limit for Hg 2+ detection is 0.18 nM, and linear range is 0.01–100 μM. The proposed terbium vanadate nanowires modified electrode exhibits significant selectivity, stability, and reproducibility toward Hg 2+ . The usefulness of the developed sensor based on the terbium vanadate nanowires modified electrode was verified by Hg 2+ detection in real samples.
研究领域:
材料科学
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纳米技术
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分析化学
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环境监测
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电化学
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 8
·1-11
·
IF 5.7
Zhang Ziyi,Liu Dan,Zhang Xiaoshuo,Luo Xueli,Lin Wanmei,Li Zhonghong,Huang Jihong
Novel methods for high-performance detection of Hg 2+ in seafood are critical for ensuring food safety and human health. Herein, Ag nanoparticles (Ag NPs) were successfully deposited on carbon microspheres (CMs) to form Ag NPs-CMs nanocomplex. The proposed Ag NPs-CMs could oxidize colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidation state TMB (oxTMB) in the presence of hydrogen peroxide (H 2 O 2 ) and had a significant UV–vis absorption peak at 652 nm. The excellent peroxidase-like activity was attributed to the increased electrostatic attraction of CMs and the catalytic synergistic effect. After adding Hg 2+ , the catalytic activity of Ag NPs-CMs was specifically enhanced and the Michaelis–Menten constant ( K m ) decreased from 0.067 to 0.052 mmol/L due to the formation of Ag-Hg amalgam which produced more superoxide anions (O 2 •− ) and hydroxyl radicals (•OH). The linear response ranges for Hg 2+ were 2~833 nmol/L and 2.5~40 μmol/L, with the low detection limit of 1.10 nmol/L. This method was applied to detect Hg 2+ in seafood with satisfactory recoveries of 95.65~106.56%. A hydrogel kit was designed for portable detection of Hg 2+ , and the response range was 0.5~5 μmol/L. This work provides a reliable method for visual Hg 2+ detection in seafood as well as a feasible strategy for the design of high-performance nanozymes. Graphical
研究领域:
纳米技术
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环境监测
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食品安全
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生物传感器
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催化
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JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
·
2017
·Vol. 55
·期 6
·1067-1076
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IF 2.952
Dalong Zhai,Wei Shi,Yonghai Hui,Zhengfeng Xie
High-efficiency, mild-conditioned tandem Knoevenagel–Michael reaction was utilized to post-modify aldehyde-containing, triphenylamine-based precursor conjugated polymer ( CP1 ) to afford dimedone-decorated aimed polymer ( CP2) . The chemical structure of CP2 was verified by FTIR and 1 H NMR analyses. With the introduction of aqueous Hg 2+ , fluorescence of CP2 in THF-water mixture ( V THF / V water = 1/100) (buffered with 5 mM sodium dihydrogen phosphate-disodium hydrogen phosphate (PB), pH = 7.4) altered significantly, with the emission changed from blue to orange. Besides this, CP2 also displayed specific optical response to ClO − in another probing medium ( V THF / V water = 1/100, buffered with 50 mM PBS (with NaCl in PB, pH = 7.4). The detailed probing process and the plausible detection mechanism of CP2 to Hg 2+ and ClO − were systematically investigated here. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017 , 55 , 1067–1076
研究领域:
高分子化学
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材料科学
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分析化学
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环境监测
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光学传感
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2015
·
IF 3.453
Lijuan Feng,Jie Sha,Yao He,Shaopeng Chen,Bingxin Liu,Haixia Zhang,Changli Lü
A novel fluorescence resonance energy transfer (FRET)-based ratiometric nanosensor is reported for toxic Hg 2+ ions detection. Poly(p-phenylenevinylene) (PPV) chain was encapsulated into the pores of mesoporous silica nanoparticles (MSNs) by ion-exchange and in situ polymerization , and used as the energy-transfer donor. A spirolactam rhodamine-B derivative (SRhB), as an ion recognition element and energy-transfer acceptor, was embedded in the pores of MSNs to form PPV@MSN@SRhB as the nanosensor . The presence of Hg 2+ in the dispersion solution of nanoparticles can induce the ring-opening reaction of the SRhB moieties and lead to the occurrence of FRET process , affording the nanoparticle system a ratiometric sensor for Hg 2+ ions. The nanoparticle sensor can selectively detect Hg 2+ ions with a detection limit of 200 nM (ca. 40 ppb). It has been found that the fabricated FRET-based scaffold with the conjugated polymer as a donor exhibited high environmental stability and was more preferred for the accurate ratiometric sensing . Moreover, the FRET-based ratiometric nanosensor can be applicable in a relatively wide pH range (pH = 5–8) in solution.
研究领域:
分析化学
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纳米生物技术
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环境毒理学
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分子传感
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材料科学
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INDUSTRIAL CROPS AND PRODUCTS
·
2026
·
IF 6.4
Zhengyun Xie,Like Ning,Farzad Seidi,Shicun Jin,Zhulan Liu,Chao Liu,Huining Xiao
Functionalized nanosilica is used to carry agrochemical IAA via disulfide bonds. • Under GSH environment, SiO 2 -S-S-IAA exhibits significant redox responsiveness. • The -SH on the surface of SiO 2 can form complexes with various heavy metal ions. • Dual-functional nanocarrier allows sustained IAA release and capture of metal ions. Owing to the low efficiency and lack of target specificity in the application of agrochemicals, e.g., plant growth regulators, the resulting environmental pollution and resource waste have raised enormous concerns over environments and human health. Herein, a redox-responsive nanosilica carrier (SiO 2 -S-S-IAA) was developed for efficient release of 3-indoleacetic acid (IAA), by functionalizing the surface of nanosilica with 3-mercaptopropyltrimethoxysilane (MPTMS), followed by the covalent coupling of IAA via redox-cleavable disulfide bonds. Under the high level of glutathione (GSH) in the plant microenvironment, IAA was released in a controlled way through disulfide bond cleavage, thus achieving redox response. In vitro controlled-release experiments confirmed that the release amount of IAA was significantly higher in the environment of 10 mM GSH, more than 10 folds at pH 5.5. Meanwhile, the thiol groups generated after release could form complexes with various heavy metal ions (Hg 2+ , As 3+ /As 5+ , Cd 2+ , Pb 2+ ), with the highest adsorption efficiency towards Hg 2 + up to 57.95%. More importantly, in planta experiments under cadmium stress have demonstrated that SiO 2 -S-S-IAA not only promoted rice seedling growth but also significantly reduced Cd 2+ accumulation in shoots, effectively inhibiting its transfer to edible parts. This work establishes a dual-functional nanocarrier platform that combines targeted agrochemical delivery with in situ heavy metal immobilization, offering a promising strategy for sustainable and precise crop protection. Download: Download high-res image (185KB) Download: Download full-size image
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TRENDS IN BIOTECHNOLOGY
·
2026
·
IF 14.9
Nan Li,Kaiyue Wei,Yuyang Zhang,Hong Chen,Jiao Li,Jianan Sun,Sai Wang,Xiangzhao Mao
Biosensors, particularly aptasensors, play a pivotal role in rapid diagnostics, but their practical performance is limited by suboptimal aptamers and conventional aptamer-material combination-based approaches. To overcome the limitations, we drew inspiration from living systems by pioneering an immune-inspired biomimetic strategy that harnesses V(D)J recombination for aptamer engineering. The resulting topology-refactored aptamers demonstrate a 133.3-fold enhancement in binding affinity. Target-aptamer binding precisely regulates the self-assembly of DNA nanosphere-Cu(II) biomimetic nanozymes, establishing the core biosensing mechanism. Integrated with smartphone-based colorimetric analysis, this platform enables high-throughput screening of various trace food contaminants in real-world complex samples, achieving high accuracy and an ultra-low limit of detection [342.1 pM for enrofloxacin, 330.8 pM for ciprofloxacin, 116.3 pM for okadaic acid, and 104.0 pM for cadmium, respectively], demonstrating broad detection capability. By redefining aptamer design, this biomimetic strategy opens new avenues for developing robust, nature-inspired biosensors for real-world use.
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JOURNAL OF CHEMICAL RESEARCH
·
2023
·
IF 1.4
Bin Gao,Yanhua Wang
The selective hydrogenation of chalcone to dihydrochalcone under atmospheric hydrogen pressure is investigated using a thermoregulated phase-transfer ruthenium nanocatalyst, representing the first ruthenium nanocatalyst used for this reaction. Under the optimal conditions, the conversion of chalcone into dihydrochalcone and the selectivity are 98% and >99%, respectively. The ruthenium catalyst can be easily separated from the product and recycled three times without any decrease in the conversion or selectivity. The turnover frequency is higher than that of a reported ruthenium complex catalyst. The thermoregulated phase-transfer ruthenium nanocatalyst is also applied for the atmospheric hydrogenation of other α,β-unsaturated ketones.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Chang Liu,Yuye Li,Ting Chen,Shuyun Meng,Dong Liu,Daming Dong,Tianyan You
Efficient preconcentration is critical for sensitive and selective electrochemical detection of metal ions, but rapid specific enrichment with depressed absorption of interfering ions at the electrode is challenging. Here, we proposed an electric field-induced specific preconcentration to boost the analytical performance of DNA-based electrochemical sensors for Hg2+ detection. As for such preconcentration, a positive external electric field was first used to enrich Hg2+ at an electrode assembled with T-rich DNA, thus boosting T–Hg2+–T recognitions. The following applied inverse electric field strips the nonspecifically absorbed Hg2+ and other interfering ions, thus depressing matrix interferences via self-cleaning. Based on this principle, we designed a portable device to realize programmable control of electric fields; a T–Hg2+–T recognition-based electrochemical sensor was thus fabricated as a model platform to assess the feasibility of electric field-induced preconcentration. The experimental results revealed that such a strategy decreased the time of T–Hg2+–T-based recognition from 60 to 20 min and led to detection with better reproducibility by depressing the influence of free Hg2+ as well as interfering ions. This strategy offered Hg2+ detection limits of 0.01 pM─three-fold better than that without preconcentration─within 22 min. The proposed preconcentration strategy offers a new way to enhance the analytical performance of sensing at the solid–liquid interface.
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JOURNAL OF CHEMICAL RESEARCH
·
2022
·
IF 1.097
Chuanchao Liu,Yanhua Wang
A ruthenium nanocatalyst is utilized for the first time for the highly efficient and selective hydrogenation of 1,5-cyclooctadiene under atmospheric hydrogen pressure. Under the optimized reaction conditions, the conversion of 1,5-cyclooctadiene and the selectivity for cyclooctene are >99% and 95%, respectively. The turnover frequency is 451 h−1, which is higher than that ever reported for Ru complex catalysts.
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ANALYST
·
2022
·Vol. 147
·期 11
·2575-2581
·
IF 5.227
Xu-Hua Zhao,Xiao-Chun Dai,Ya-Nan Zhou,Han-Xiao Zhang,Xiao-Hua Cui,Xiang Zhai,Bao-Feng Yu,Zhi-Ling Song
In this paper, we developed an amplified fluorescence biosensor for acetylcholinesterase (AChE) activity detection by taking advantage of the mercury ion-mediated Mgzyme (Mg2+-dependent DNAzyme) activity. The catalytic activity of Mgzyme can be inhibited by the formation of T–Hg2+–T base pairs between the Mgzyme and mercury ions. Therefore, the Mgzyme–Hg2+ complex has no activity on a molecular beacon (MB) substrate, which afforded a very weak fluorescence background for this biosensor. After the addition of acetylcholinesterase (AChE), the substrate acetylthiocholine could be hydrolyzed to thiocholine, which has a stronger binding power with mercury ions than T–Hg2+–T base pairs. Therefore, the Mgzyme activity was recovered. The activated Mgzyme could hybridize with the MB substrate and undergo many cleavage cycles, resulting in a significant increase of fluorescence intensity. This biosensor displayed high sensitivity with the detection limit as low as 0.01 mU mL−1. Moreover, this design did not require complex composition and sequence design; thus it is simple and convenient. This biosensor was also applied for the determination of AChE in human blood and showed satisfactory results.
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Food Science & Nutrition
·
2022
·Vol. 10
·期 6
·1841-1853
·
IF 3.553
Jun Yang,Shulin Hu,Anqi Liao,Yetian Weng,Shuli Liang,Ying Lin
Current chemical analysis approaches for contaminants have failed to reveal their biotoxicity. Moreover, conventional bioassays are time consuming and exhibit poor repeatability. In this study, we performed the acute toxicity detection of various contaminants (chromium (Cr), cadmium (Cd), lead (Pb), arsenic (As), mercury (Hg), tin (Sn), nickel (Ni), and bisphenol A (BPA)) with four bioluminescent bacteria ( Vibrio qinghaiensis Q67, V. fischeri , Photobacterium phosphoreum T3, and P. phosphoreum 502) using a rapid, flexible, and low-cost bioassay. We found that the temperature affected the bacterial luminescence, and freeze-dried cells exhibited sensitive toxic responses to contaminants. Indeed, the optimized protectants containing 12% (w/v) trehalose, 4% sucrose, and 2% sorbitol displayed better luminescence and toxic sensitivity. Furthermore, freeze-dried powders of these strains were prepared and subjected to acute toxicity detection. The results showed that all contaminants exhibited acute toxicity toward Q67, but the other strains did not show obvious response to nickel and tin. The relative half-maximal effective concentration (EC 50 ) values of BPA, Cr, Cd, Pb, As, Hg, Ni, and Sn to Q67 were 0.674, 1.313, 11.137, 5.921, 4.674, 0.911, 5.941, and 54.077 mg/L, respectively. In addition, the EC 50 values of contaminants toward different strains were suggested to be statistically significant. Freeze-dried Q67 exhibited toxic responses to more contaminants than the other bioluminescent strains; therefore, Q67 was selected to be more suitable than the other strains for single and mixture toxicity detection tests. Compared with other strains, Q67 was more appropriate for the rapid screening of the mixture toxicity of contaminants in samples as a nonspecific screening sensor before the use of standard analysis approaches.
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Libing Liu,Lei Du,Sen Lu,Bo Yang,Xu Zhao,Deli Wu,Xunchang Fei,Hongping He,Dongsheng Wang
Various combined processes with pre-coagulation have been developed for biologically treated landfill leachate , but the microscopic-level processes occurring during coagulation remain largely unknown. Herein, dissolved organic matter (DOM) fate using fluorescence excitation emission matrix spectroscopy combined with parallel factor analysis and electrospray ionization coupled Fourier transform-ion cyclotron resonance mass spectrometry and concomitant heavy metal (HM) behaviors were explored at the molecular level. In addition, AlCl 3 and two polyaluminum chloride (PACl) species (dominated by [AlO 4 Al 12 (OH) 24 (H 2 O) 12 ] 7+ and [(AlO 4 ) 2 Al 28 (OH) 56 (H 2 O) 26 ] 18+ , respectively) were used. The results show that all coagulants are efficient at removing DOM. PACl was found to be advantageous over AlCl 3 in overcoming pH fluctuation, which is ascribed to the different dominant mechanisms, namely, entrapment and sweep flocculation for AlCl 3 and charge neutralization for PACl. Consequently, PACl was more effective at removing humic substances , usually high-molecular-weight, oxygen-rich and unsaturated, than protein substances. For HM removal, PACl was likewise better and more stable, where As, Cu, Ni, Co and Hg were removed predominantly via adsorption, and Cr, Zn, Pb, Cd and Mn were removed via coprecipitation . Correlation analysis showed that humic substances tended to complex with HMs and be removed synergistically due to the ubiquitous occurrences of aromatic structures and oxygen-containing functional groups.
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