Catalysts
·
2024
·Vol. 14
·期 8
·473
·
IF 3.8
Hong Zhang,Zhi Wang,Fengxi Li,Lei Wang,Bo Ren
A stable and efficient biocatalyst was prepared by encapsulatingTrametes versicolorlaccase using an acrylic acid-grafted β-cyclodextrin hydrogel (Lac-CD-PAA). Scanning electron microscopy and nitrogen adsorption-desorption experiments showed that there were regularly distributed channels in the spongy Lac-CD-PAA. In addition, a large number of mesopores and macropores existed in the wall of the hydrogel lamellae. This network structure reduced the diffusion resistance of the hydrogel to the target substrate. The relative activity of the resulting Lac-CD-PAA could be maintained at 35.8% after six cycles of use. Lac-CD-PAA exhibited higher thermal and chemical stability compared to free laccase. The negative charge on the surface of Lac-CD-PAA gives it the ability to pretreat cationic dyes. In six consecutive methylene blue decolorization tests, Lac-CD-PAA decolorized better than free laccase. The results showed that the prepared β-cyclodextrin-based composite hydrogel was a good carrier for laccase.
✓ 已验证
产品关联
DOI
高影响因子
FIBERS AND POLYMERS
·
2024
·
IF 2.2
Zhang Qi,Yang Ce,Gu Mingqi,Qin Shaoxuan,Yin Jianhua,Wang Xiangrong,Zhang Wei
The integration of natural dyes and biomass-based dyeing auxiliaries holds huge potential to address the environmental sustainability challenges, which the textile industry encounters. This research employed Portulaca oleracea L. extract as a natural dye, utilizing laccase as a catalyst for polymerizing the dye, thereby creating a precursor that enhances its eco-friendly dyeing attributes on cotton fabrics. The initially exhibited suboptimal direct dyeing performance on cotton fabrics, resulting in a faint yellow hue and a K/S value of 0.66. However, following laccase catalysis, the depth of color on the dyed fabric significantly increased, with a K/S value reaching 4.41, and the dye uptake rate increased from 14.54% to 33.69%. The resulting dyed fabric exhibited excellent fastness to soap washing, light, and rubbing, all scoring above levels 4 to 5. Analysis of dyeing kinetics and adsorption models indicated that the dyeing process was consistent with both the pseudo-first-order and pseudo-second-order kinetic models, suggesting physical and chemical bonding between the precursor and the fiber. Further investigations employing ultraviolet spectrum analysis, matrix-assisted laser desorption/ionization time of flight mass spectrometry, and gas chromatography-mass spectrometer revealed the fundamental composition of the natural dye, the molecular weight, and structural characteristics of the precursor, substantiating that the catalytic mechanism involves laccase-catalyzed oxidation. Additionally, the dyed cotton yielded an ultraviolet protection factor value of 230 and a long-lasting herbal fragrance, accurately identified by an electronic nose. These results confirm the viability of intensifying fabric color using the laccase-catalyzed system. This technique significantly expands the environmental applications of its extract.
✓ 已验证
产品关联
DOI
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2024
·
IF 6.5
Guolin Zheng,Yifan Cui,Zhe Jiang,Wenda Wang,Man Zhou,Yuanyuan Yu,Ping Wang,Qiang Wang
To our knowledge, surface hydrophobicity is normally fabricated by hydrophobic or fluorinated materials; however, it can be predicted. So, it is exciting and high value that using a single hydrophilic material directly obtains a hydrophobic surface , which may be a promising and significant aspect and a great challenge in surface and interface science . Herein, the copper sulfide (CuS) nanoflowers were in-situ deposited on the cotton fabric after cotton fabric was incubated with copper(II) chloride dihydrate and thioacetamide stirring for 3 h at 80 °C, labelled as Cot@PTA@CuS. Then, Cot@PTA@CuS was treated by near infrared (NIR) laser irradiation at 808 nm, recorded as Cot@PTA@CuS-NIR. Interestingly, Cot@PTA@CuS-NIR exhibits good hydrophobicity with WCAs of 136.5 ± 3.5° and hydrophobic stability within 30 min. Notably, the hydrophobicity of Cot@PTA@CuS-NIR is created without using the lower surface free energy materials, possessing significant progressiveness compared with conventional surface hydrophobicity fabricated by hydrophobic or fluorinated materials. The X-ray diffraction (XRD) and X-ray photoelectron spectra (XPS) demonstrate the formation of cuprous oxide (Cu 2 O) after NIR laser irradiation. Subsequently, the polydimethylsiloxane (PDMS) was selected to encapsulate CuS nanoflowers with improving the stability of CuS nanoflowers and obtaining superhydrophobicity, recorded as Cot@PTA@CuS-NIR@PDMS, achieving the results of killing two birds with one stone. Cot@PTA@CuS-NIR@PDMS also has an excellent photothermal effect and UV resistance. Finally, Cot@PTA@CuS-NIR@PDMS was used for efficient oil/water separation. Overall, this work renders a novel, feasible, and facile idea for gaining surface hydrophobicity by hydrophilic CuS nanoflowers with NIR laser irradiation, inspiring more scholars to create surface hydrophobicity by hydrophilic materials.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Guolin Zheng,Zengdi Zhao,Aming Wang,Haonan Cheng,Xinjun Bao,Hengshu Zhou,Dan Zhang,Qiang Wang
Inspired by lotus leaf, biomimetic hydrophobic cellulose fiber-based textiles were usually fabricated by the lower surface energy materials. However, it is rare to witness a hydrophobic surface obtained by near infrared laser (NIR) irradiation a superhydrophilic nanomaterial, which remains a huge challenge. Herein, the CuS nanospheres was in-situ anchored on the surface of gallic acid self-polymer treated cotton fabric. Luckily, the excellent hydrophobicity of CuS nanospheres coating (contact angles of 142.7 ± 2.8°) was for the first time demonstrated after a NIR laser irradiation with 1.5 W/cm 2 for 8–10 s. Notably, the novel hydrophobicity exhibits a significant advance compared with traditional hydrophobicity prepared by lower surface energy materials. Then, the laser-mediated hydrophobic mechanism was demonstrated by the cold-field emission scanning electron microscope (FESEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and thermal imager. Moreover, the dynamic contact angle revealed the uniform hydrophobicity. Furthermore, the hydrophobic nanospheres coating possessed good photothermal effect and UV resistance. Overall, this work renders a novel, feasible, and efficient strategy to rapidly obtain the hydrophobic surface by NIR laser-induced the superhydrophilic CuS nanospheres coating.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Lijia Li,Xumei Feng,Mengjie Geng,Fei Teng,Yang Li
The advancement and utilization of functional foods hold the capacity to efficaciously enhance human nutrition and well-being. However, within the present epoch characterized by recurrent food safety and quality concerns, there exists a paucity of suitable food systems that are capable of concurrently amalgamating the merits of nutritional enrichment and high stability. Therefore, in this study, heteroprotein aggregates were synthesized with modified-soy lipophilic protein (SLP) and lysozyme (LY) as the main components. Subsequently, an interpenetrating network (IPN) was constructed using sugar beet pectin (SBP) and heteroprotein aggregates to form W-O-W emulsion gels. The heteroprotein aggregates formed by SLP and LY provided a stable oil-water interface, allowing the IPN to maximally confine the internal oil droplets and improve the mechanical properties and water retention capacity of the W-O-W emulsion gels. Meanwhile, the W-O-W emulsion gels also possessed pH-responsiveness, endowing them with the ability to modulate their self-morphology and network structure in accordance with pH fluctuations. Moreover, they possessed outstanding protective and slow-release efficacies, which were conducive to the targeted release of the delivered substances. Thus, W-O-W emulsion gels based on heteroprotein aggregation and SBP show great potential as an option for functional food applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Guobao Ning,Huan Liang,Linjiang Guo,Xiaokang Lu,Lin Xiao,Tianle Qi,Hui Zhao,Can-Peng Li
The application of single-atom nanozymes in the removal of organic dye pollution by advanced oxidation process (AOPs) have been widely concerned. However, the application of single-atom nanozymes in AOPs is rarely reported. In this work, a kind of Fe-and nitrogen-codoped carbon (Fe-N-C) with peroxidase-like and laccase-like activities was prepared by calcining Fe-doped MOF, then the chiral peptides were introduced into single-atom nanozymes, and chiral single-atom nanozymes (L-Au@FeNC) were successfully prepared. The L-Au@FeNC can effectively activate PMS to degrade different kinds of organic dyes and also shows high cycle stability. In addition, the results of quenching experiment and electron paramagnetic resonance (EPR) test show that 1 O 2 produced during PMS activation plays an important role in the degradation of organic dyes, and O 2 ·- , ·OH, and SO 4 ·- are also produced during PMS activation, suggesting that the degradation of selected organic dyes include radical and non-radical pathways. It should be noted that L-Au@FeNC showed higher activity than D-Au@FeNC in enzyme-like activity (laccase-like and peroxidase-like) experiment and organic dye degradation, the results of molecular docking simulation indicated that it may be due to the better binding force of L peptide to the substrate of enzyme-like reaction and organic dye than D peptide. This chiral single-atom nanozymes provide a new idea for the application of organic dye degradation and enzyme-like catalysis design.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Zuwei Yu,Xue Zhang,Shiyang Li,Jun Yang,Muci Wu,Qian Wu,Jingyi Wang
To obtain efficient natural food packaging materials, we utilized acorn starch (AS)-based film strengthened by feruloylated arabinoxylan (FAX) gel and additional retrogradation treatment to extend the shelf life of Agaricus bisporus ( A. bisporus ). Fourier transform infrared spectroscopy (FT-IR), confocal laser scanning microscopy (CLSM), and scanning electron microscopy (SEM) analyses showed that due to the strong hydrogen bonding between FAX and starch molecules, physical crosslinking occurred between FAX and starch molecules in the composite film, and the microstructure became more compact. Thermogravimetric, tensile strength and swelling degree analyses indicate that the composite film exhibits better thermal stability, mechanical properties, and waterproofing compared to the pure AS film. Consequently, after five days of storage, the moisture content of the A. bisporus packaged with our composite film was 7.53 times and 5.73 times higher than that of the control group and the commercially available PEF group, respectively. Moreover, it delayed the respiration or transpiration of A. bisporus (lower weight loss, relative conductivity, MDA content). This packaging film developed with the objective of eco-friendly and biodegradability has considerable application potential in food and other industries.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Technology & Innovation
·
2024
·
IF 6.7
Zhao Li,Kanghui Ning,Ning Guo,Yuanyuan Shao,Song Li,Daoji Wu,Xiangzheng Ren,Lin Wang
Tetracycline (TC) antibiotics have been widely used in aquaculture and medical industries owing to their low cost, high efficiency, and broad-spectrum characteristics. However, it is difficult to degrade naturally in the environment, leading to its large accumulation in wastewater. The enzymatic degradation method using laccase exhibits good application potential in TC removal. In this study, low-cost activated carbon (AC) was used as the carrier to immobilize laccase using the adsorption–cross-linking method, forming Laccase@AC, characterized by high efficiency, eco-friendliness, and cost-effectiveness. Laccase@AC was used to treat TC-contaminated water. Its stabilities and catalytic activity for TC were comprehensively investigated. The results showed that Laccase@AC exhibited high enzyme loading capacity (407.97 mg/g), activity retention (1.36 U/mg), and stabilities. Moreover, Laccase@AC exhibited a higher TC removal efficiency than free laccase. The removal rates of 20 mg/L of TC using Laccase@AC were 78.7 % and 99 % after 6 h and 24 h, respectively, in the presence of a mediator (syringaldehyde, SA), whereas only 14.9 % of TC was removed using the same amount of free laccase. Temperature and TC concentration affected the performance of Laccase@AC. The TC optimal removal rate was achieved at 40–60℃ using 1 mg of Laccase@AC per 15 mg TC. Degradation product analysis revealed that catalytic oxidation was the main reaction catalyzed by laccase and enhanced by SA. The AC provided a stable reaction substrate and enhanced adsorption capacity. Meanwhile, the biocatalytic system effectively degraded TC and produced downstream products with low toxicity, demonstrating its potential for treating antibiotic wastewater using immobilized laccase. Therefore, immobilized laccase is an efficient biocatalyzer for eliminating TC in wastewater, and it has a broad application prospect in the treatment of antibiotic-contaminated wastewater.
✓ 已验证
产品关联
DOI
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Lin Wang,Tianxin Yuan,Yan Zhang
A time-temperature integrator (TTI) based on laccase@Cu 3 (PO4) 2 nanoflowers (laccase@NFs) was created to monitor the freshness of pasteurized milk during storage. To address the challenges of easy inactivation, poor stability, and high use-cost of laccase in the application of TTI, laccase@NFs were synthesized by ultrasonic-assisted biomineralization. The laccase@NFs-based TTI was created through the enzymatic reaction between laccase and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid ammonium salt), and the effects of laccase@NFs addition amount on the reaction rate, discoloration lifetime and activation energy (Ea) were discussed. Furthermore, the spoilage pattern and kinetic properties of pasteurized milk were explored based on titratable acidity. The measurement of the Ea was determined as 49.98 kJ/mol, and an investigation was conducted to assess the suitability of the TTI with pasteurized milk under both constant and variable temperature conditions. This research aims to contribute valuable insights into the application of enzymatic TTI in monitoring the shelf life of pasteurized milk.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Jishao Jiang,Huilin Cui,Zhuyu Tang,Shipeng Wang,Ke Cheng,Chunyan Zhang,Tonghuan Yu,Jinghua Lv,Fuqiang Yu,Dong Liu
This study investigates the effects of biochar, laccase, and their combined application on nitrogen transformation, the degradation of sucralose (SUC) and acesulfame (ACE), and the dynamics of bacterial communities during sewage sludge composting. The results indicated that NH 3 emissions were reduced by 41.2 %, 17.5%, and 40.8 % in the biochar, laccase, and biochar-laccase treatments, respectively, compared to the control. Meanwhile, N 2 O emissions decreased significantly by 26.8 % and 16.0 % for the biochar and biochar-laccase treatments, respectively, but increased by 8.1 % for the laccase treatment. Additionally, the biochar, laccase, and biochar-laccase treatments enhanced the degradation of SUC and ACE by 36.6–68.3 % and 49.2–69.8 %, relative to the control, respectively. Network analysis showed that the additive-treatment enhanced the cooperation within bacterial communities, and solidified artificial sweeteners degradation, especially under biochar-laccase application. Phylogenetic investigation of communities by reconstruction of unobserved states further indicated that biochar, laccase, biochar-laccase treatment increased enzymes associated with the degradations of organic matter and artificial sweeteners. In conclusion, it suggested that a combined addition of biochar and laccase was an effectively way to reduce nitrogen loss and promote artificial sweeteners degradations during sewage sludge composting.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Jishao Jiang,Zhuyu Tang,Kai Liu,Shipeng Wang,Yunbei Li,Jinghua Lv,Xu Yan,Dong Liu,Parag Bhople,Ke Cheng
Laccase plays a crucial role in eliminating pharmaceuticals and personal care products (PPCPs) in different ecological system, but it could be easily decomposed or inactivated under high temperature and pH conditions during the composting process. In this work, three treatments, including Control, LAC-0 and LAC-8 (the two-stage laccase application on day 0 and 8), were investigated the effects of two-stage laccase application on the typical PPCPs degradations, also the laccase activity and the successions of bacterial community. Results revealed that two-stage laccase application significantly increased the NO 3 -N contents by 12.6 % and the TN content by 28.0 %, respectively. Meanwhile, the two-stage laccase application significantly increased the TOC degradation, and the enzymatic activities of laccase, cellulase, and urease, especially during the cooling and mature stages. In addition, the degradation rate of carbamazepine for LAC-0 and LAC-8 treatments increased by 44.7 % and 54.5 %, and ibuprofen by 64.5 % and 71.1 % relative to control, respectively. Microbial correlation analysis showed that the two-stage laccase application (LAC-8) facilitated the mutualistic relationship among the bacteria communities, and increased the negative correlations between the bacterial communities and PPCPs degradations. Furthermore, the partial least squares path model indicated that bacterial community played a directly role in the PPCPs degradation, and environmental factors and enzyme activities had an indirectly roles.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Siyu Wu,Xiaojuan Niu,Jiali Chen,Wangni Meng,Han Tao,Jianli Zhou,Yuangen Wu
Kanamycin (KAN) can generate a serious threat to human health due to its tendency to accumulate in food. Current colorimetric strategy for KAN detection still faces some challenges, and nanozyme-based colorimetry is an ideal alternative approach. Herein, a non‑copper-based multivalent laccase mimic named Mn-Car is first synthesized through a self-assembly of manganese ions and the endogenous biomolecule carnosine, which is further used to develop a novel colorimetric method for ultrasensitive and selective detection of KAN. The laccase-like catalytic center is formed through the multidentate chelation bonds between manganese ions and imidazolium groups of histidine as well as carboxyl groups of β-alanine in carnosine. The multivalent oxidation states of manganese dynamically regulate electron transfer pathways through synergistic coordination. The unique Mn-Car architecture drives the efficient generation of superoxide radicals (O 2 · − ) and semiquinone radicals (SQ· − ). Leveraging the excellent laccase-like activity of Mn-Car , a highly sensitive colorimetric method for KAN detection is developed. Mn-Car forms polydentate chelation with the amino and hydroxyl groups in KAN, which can trigger multi-step cascade oxidation reactions that disrupt the structural core of Mn-Car and lead to reduced colorimetric signals. The colorimetric method achieves a limit of detection (LOD) of 0.102 μM and is successfully applied for on-site detection of KAN in water samples and honey, providing a novel platform for antibiotic residue analysis.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）