INDUSTRIAL CROPS AND PRODUCTS
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2024
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IF 5.6
Xuanhao Huang,Xinyi Guo,Yao Meng,Shaobo Lou,Xinxing Wu,Yan Zhang,Renzhong Wei,Shuaibo Han,Fangli Sun,Hui Wang
Bamboo, which has excellent physical properties and environmental credentials, is widely used in the construction industry and furniture manufacturing. Bamboo is, however, susceptible to attack by molds and bacteria; protection by natural phenolic antimicrobial agents would be a green and environmentally friendly solution to this problem. The application of such protection has, so far, been hampered by poor stability and ease of volatilization of the antimicrobial agent. Here, an innovative strategy that uses the catalytic action of the laccase to chemically attach a natural antimicrobial agent, eugenol (EU), to bamboo, is described. This modification endows bamboo with both long-lasting and extensive mold resistance and antibacterial activity. Laccase-catalyzed covalent bonding of EU to bamboo reduces the volatilization rate of the EU from 1.72 % to 0.29 %. The outstanding mold resistance and antimicrobial activity of bamboo treated using this modified process is retained during a 50-day mold resistance test, with mold growth inhibited by as much as 87.5 %. The treated bamboo is also able to inhibit the growth of both Gram-positive and Gram-negative bacteria. The antimicrobial mechanism results shown that the bamboo lignin is the primary reaction site during laccase-catalyzed EU modification of bamboo, and the long-term mold resistance derived from modified lignin. Furthermore, we analyzed bacterial morphology through SEM, and the results demonstrated that modified enzymatic hydrolysis lignin (EHL) significantly disrupts bacterial morphology, including membrane invagination and collapse. This study provides a new direction for the green development of mold- and bacteria-resistant bamboo, which has potential for use in a wide range of application.
研究领域:
材料科学
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微生物学
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植物化学
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DOI
高影响因子
Scientific Reports
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2016
·Vol. 6
·期 1
·1-7
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IF 5.228
Jin Xiaoting,Yu Xiangyang,Zhu Guangyan,Zheng Zuntao,Feng Fayun,Zhang Zhiyong
A high capacity of laccase from Trametes versicolor capable of degrading pesticides has been revealed. The conditions for degrading of five selected pesticides including chlorpyrifos, chlorothalonil, pyrimethanil, atrazine and isoproturon with the purified laccases from Trametes versicolor were optimized. The results showed that the optimum conditions for the highest activity were pH at 5.0 and temperature at 25 °C. The best mediators were violuric acid for pyrimethanil and isoproturon, vanillin for chlorpyrifos, and acetosyringone and HBT for chlorothalonil and atrazine, respectively. The laccase was found to be stable at a pH range from 5.0 to 7.0 and temperature from 25 to 30 °C. It was observed that each pesticide required a different laccase mediator concentration typically between 4.0–6.0 mmol/L. In the experiment, the degradation rates of pyrimethanil and isoproturon were significantly faster than those of chlorpyrifos, chlorothalonil and atrazine. For example, it was observed that pyrimethanil and isoproturon degraded up to nearly 100% after 24 hours while the other three pesticides just reached up 90% of degradation after 8 days of incubation.
研究领域:
环境生物学
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酶学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2019
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IF 4.527
Yiping Feng,Mengyao Shen,Zhu Wang,Guoguang Liu
In this study, we investigated the transformation of atenolol (ATL) by the naturally occurring laccase from Trametes versicolor in aqueous solution. Removal efficiency of ATL via laccase-catalyzed reaction in the presence of various laccase mediators was examined, and found that only the mediator 2, 2, 6, 6-tetramethyl-1-piperidinyloxy (TEMPO) was able to greatly promote ATL transformation. The influences of TEMPO concentration, laccase dosage, as well as solution pH and temperature on ATL transformation efficiency were tested. As TEMPO concentrations was increased from 0 to 2000 μM, ATL transformation efficiency first increased and then decreased, and the optimal TEMPO concentration was determined as 500 μM. ATL transformation efficiency was gradually increased with increasing laccase dosage. ATL transformation was highly pH-dependent with an optimum pH of 7.0, and it was almost constant over a temperature range of 25–50 °C. Humic acid inhibited ATL transformation through competition reaction with laccase. The presence of anions HCO 3 − and CO 3 2− reduced ATL transformation due to both anions enhanced solution pHs, while Cl − , SO 4 2− , and NO 3 − at 10 mM showed no obvious influence. The main transformation products were identified, and the potential transformation pathways were proposed. After enzymatic treatment, the toxicity of ATL and TEMPO mixtures was greatly reduced. The results of this study might present an alternative clean strategy for the remediation of ATL contaminated water matrix.
研究领域:
环境科学
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生物化学
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DOI
PubMed
高影响因子
CELLULOSE
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2021
·Vol. 28
·期 7
·4397-4409
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IF 5.044
Ni Shuzhen,Liu Na,Fu Yingjuan,Gao Hailong,Qin Menghua
Abstract Herein, a great potential approach initiated by laccase is applied to graft the biopolymers (chitosan and phenols) onto the unbleached Kraft pulp fibers to enhance the hydrophobicity and strength. The results showed that the chitosan and laccase/phenols exhibited a synergistic action on the significant improvement of the hydrophobicity of the KP pulp fibers. Compared with the base paper, a high sizing degree value (227.08 s) was achieved when the fibers were treated by the laccase/4-hexyloxyphenol/chitosan (Lac/HP/CTS) system. The water retention value of the corresponding KP fibers reduced from 176.8 to 171.3%, and the Kappa number of treated fibers increased from 26.8 to 29.3, confirming the successful graft of HP onto the fibers. The effect of the reaction parameters, including the temperature, pH, HP and chitosan dosage, reaction time and pulp suspension consistency on the strength and hydrophobicity was investigated. pH value was found as the most remarkable factor influencing the sizing degree and gave a highest sizing value (506.2 s) at pH 5.5. The strength performance presented an opposite trend against the hydrophobicity, since the number of formed hydrogen bonds is the least when the hydrophobicity is the best. This work is extremely significant for providing valuable reference on improving the hydrophobicity and strength performance of papers through a green and biological way. Graphic abstract
研究领域:
制浆造纸科学
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生物技术
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材料科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
·
IF 3.909
Qian Zhou,Li Cui,Lamei Ren,Ping Wang,Chao Deng,Qiang Wang,Xuedong Fan
Chitooligosaccharide (COS) has the characteristic of antioxidant and antibacterial effects. To improve the properties of silk fibroin (SF), COS was enzymatically grafted onto SF membranes using a laccase from Trametes versicolor . d -Glucosamine hydrochloride (GAH) and p -hydroxyphenylacetamide (PHAD), as the model compound of COS and tyrosine residues in SF were utilized to disclose the grafting mechanism, respectively. The data from UPLC-TQD and GPC analysis implied that laccase might catalyze the oxidation of PHAD and led to the formation of self-polymerized products. FTIR and 1 H NMR results verified the occurrence of the laccase-assisted reactions between COS and PHAD. For the fibroin samples with different treatments, incubation with laccase alone led to remarkable increase in the molecular weight of SF, mainly owing to the efficient self-crosslinks of the fibroin chains. For the COS-grafted SF membrane, there was no obvious change in the thermal behavior, while the antioxidant and antibacterial properties were evidently improved when compared to that of the untreated. Meanwhile, biocompatibility of the COS grafted SF membrane was acceptable according to the cell viability of NIH/3T3 cells. The present work provides a novel method for preparation of the multifunctional fibroin-based biomaterials.
研究领域:
生物材料学
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酶工程
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医学材料
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DOI
PubMed
高影响因子
Materials
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2016
·Vol. 9
·期 4
·282
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IF 2.728
Dawei Li,Jun Zang,Jin Zhang,Kelong Ao,Qingqing Wang,Quanfeng Dong,Qufu Wei
Carbon xerogel-zinc oxide (CXZnO) composites were synthesized by a simple method of sol-gel condensation polymerization of formaldehyde and resorcinol solution containing zinc salt followed by drying and thermal treatment. ZnO nanoparticles were observed to be evenly dispersed on the surfaces of the carbon xerogel microspheres. The as-prepared CXZnO composites were mixed with laccase (Lac) and Nafion to obtain a mixture solution, which was further modified on an electrode surface to construct a novel biosensing platform. Finally, the prepared electrochemical biosensor was employed to detect the environmental pollutant, catechol. The analysis result was satisfactory, the sensor showed excellent electrocatalysis towards catechol with high sensitivity (31.2 µA·mM−1), a low detection limit (2.17 µM), and a wide linear range (6.91–453 µM). Moreover, the biosensor also displayed favorable repeatability, reproducibility, selectivity, and stability besides being successfully used in the trace detection of catechol existing in lake water environments.Keywords:carbon xerogel;ZnO;laccase;phenolic biosensor
研究领域:
材料科学
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环境科学
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生物传感
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DOI
PubMed
高影响因子
CHEMOSPHERE
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2023
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IF 8.8
Hongqing Bo,Ziyan Zhang,Zhonglin Chen,Wenrui Qiao,Siyi Jing,Tongtong Dou,Tian Tian,Ming Zhang,Weichuan Qiao
Enzymes immobilized on the surface of the carriers are difficult to maintain their conformation and high activity due to the influence of the external harsh environments. A biomimetic core-shell [email protected] bioreactor was constructed by depositing polydopamine (PDA) on the surface of the recombinant Escherichia coli with CotA laccase gene, and releasing intracellular laccase into the PDA shell using ultrasound to break the cell wall of the bacteria. The bioreactor provided a nano-confined environment for the laccase and accelerated the mass and electron transfer in the volume-confined space, with a 2.77-fold increase in Km compared with the free laccase. Since there was no barrier of the cell wall, the crystal violet dye can enter the bioreactor to participate in the enzymatic reaction. As a result, [email protected] achieved excellent decolorization performance even without ABTS as an electron mediator. Moreover, the cytoplasmic solution retained in the PDA shell promoted the enzyme's tolerance to pH, temperature and harsh environments. In addition to PDA encapsulation, carbonyl and –NH 2 groups of PDA were bound covalently with –NH 2 and –COOH on the laccase in the [email protected] , resulting in an extremely high laccase loading of 817.59 mg/g. Also, the relative activity of the bioreactor maintained approximately 75% after 10 cycles of reuse. In addition, the protection of the PDA shell increased the resistance of laccase to UV irradiation. This work provides a novel method of laccase immobilization for application in wastewater treatment.
研究领域:
生物技术
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环境科学
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DOI
PubMed
高影响因子
ACS Applied Polymer Materials
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2020
·
IF 0
Ya Liu,Li Wang,Hongsheng Lu,Zhiyu Huang
The increasing discharge of oily wastewater and frequent oil spill accidents pose a great threat to our environment and cause a great waste of valuable resources. However, most existing methods to separate oily water often involve unique equipment and toxic chemicals that hinder their commercial applications. Inspired by the synergy of various additives in commercial paint, we proposed a strategy to fabricate a metal mesh for oil–water separation by using a bicomponent hydrogel paint. This paint consists of an undercoat and a topcoat. Owing to the exceptional adhesion and molecular recognition effect, the paint can show a high binding degree. In addition, the hydrogel paint can decouple polymerization from crosslinking due to the reversible hydrogen bond crosslinking of topcoat, resulting in a separation of labor between paint makers and paint users. The rough as-prepared metal mesh with hydrophilic hydrogel and micropapillaes result in an excellent underwater superhydrophobicity and low oil-adhesion characteristics. This mesh can display high stability and durability under various complex conditions and successfully remove water from various oil/water mixtures and exhibit a high separation efficiency (above 99%). We believe that even ordinary people without professional training can also prepare an efficient oil/water separation mesh easily through brushing the bicomponent hydrogel paint. Consequently, the bicomponent hydrogel paint could be an attractive commercial paint to fabricate oil/water separation mesh.
研究领域:
环境科学
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材料科学
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化学工程
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产品关联
DOI
Journal of Environmental Chemical Engineering
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2021
·
IF 5.909
Abdul Latif,Ahsan Maqbool,Kai Sun,Youbin Si
The present work investigated the immobilization of laccase ( Trametes versicolor ) on Cu-alginate beads by entrapment method and its utilization to degrade bisphenol A (BPA) in aqueous medium. The immobilized laccase demonstrated higher storage stability as compared to free enzyme. Box-Behnken experimental design was used to optimize the immobilization conditions (sodium alginate concentration, CuSO 4 molarity, and hardening time). The optimal conditions were found to be 3% sodium alginate concentration (W/V), 0.141 mM CuSO 4 and 90 min hardening time. Experimental data exhibited that immobilized laccase (0.5 g) could degrade 96.12% of BPA (10 mg/L) at pH 5.0, 30°C, 150 rpm and 1 h of reaction. The degradation products of BPA were identified by LC-MS analysis; hence the degradation pathways were suggested. The toxicity of BPA products was judged by phytotoxicity test. Immobilized laccase could also degrade BPA in real water, hence could be considered as an efficient candidate for removing BPA in water.
研究领域:
环境科学
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生物技术
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DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Pengshen Jing,Qian Chen,Haifeng Ke,WenHui Xu,Yanhong Ni,Chun Mao,Runzhi Deng
In the fields of neuroscience and medicine, the accurate capture and quantitative analysis of dopamine (DA) levels in the blood are of great clinical significance for the early diagnosis of neurological dysfunctions and other pathological conditions. In this study, we developed a laccase (La) sensor based on micromotor technology to accurately detect DA content in blood. Firstly, the magnesium-based micromotor was used to endow the sensor with autonomous motion performance, which significantly increased the contact frequency between La and DA molecules in blood. Secondly, the micromotor-loaded Fe 3 O 4 /PPY/La magnetic nanoparticles provide an efficient electrical signal transmission interface for La, while the presence of polypyrrole (PPY) prevented electrode passivation caused by La. In addition, we used magnetic to collect Fe 3 O 4 /PPY/La after capture and tested it by differential pulse voltammetry. Finally, thanks to the excellent catalytic performance and high selectivity of La, the effective recognition and detection of DA in blood samples were realized, and an efficient electrochemical detection method was developed. This innovative detection scheme has a wide linear detection range and high sensitivity, which is expected to provide a more accurate diagnostic tool for health problems related to DA abnormalities, so as to better cope with and prevent related diseases.
研究领域:
神经科学
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医学
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生物传感技术
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiaodan Wu,Changjun Cai,Qingjing Cen,Guiming Fu,Xuan Lu,Hongli Zheng,Qi Zhang,Xian Cui,Yuhuan Liu
Laccase is a green catalyst that can efficiently catalyze phenolic pollutants, and its catalytic efficiency is closely related to the interaction between enzyme and substrates. To investigate the binding effects between enzyme and phenolic pollutants, phenol, p-chlorophenol, and bisphenol A were used as substrates in this study. We focused on the removal and catalytic mechanism of these pollutants in water using yellow laccase derived from Coriolopsis gallica . The enzymatic catalytic products were characterized using Ultraviolet-Visible Absorption Spectroscopy (UV–Vis), Fourier Transform Infrared Spectroscopy (FTIR), and High-Resolution Mass Spectrometry (HRMS), and the catalytic mechanism of laccase on phenolic pollutants was further explored by molecular docking. Based on the structural characterization and molecular docking results, the possible polymerization pathways of these phenolic compounds were speculated. Laccase catalyzed phenol to produce phenolic hydroxyl radicals, their para-radicals, and ortho-radicals, which polymerized to form oligomers linked by benzene‑oxygen-benzene and benzene-benzene. P-chlorophenol produced phenolic hydroxyl radicals and their ortho-radicals, polymerizing to form oligomers connected by benzene‑oxygen-benzene or benzene-benzene. The C C bond of the isopropyl group of bisphenol A broke to formed an intermediate product, which was further polymerized to formed a benzene‑oxygen-benzene linked oligomer.
研究领域:
环境生物学
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酶催化
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污染治理
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2024
·
IF 7.6
Kai Sun,Zi-Hao Wu,Jie Liu,Zhou-Hua Cheng,Dong-Feng Liu,Hui Lin,Han-Qing Yu
Fungal laccase producers can effectively address bisphenol A (BPA) and antibiotic-contaminated water. However, the role of small mediators produced by fungal secondary metabolism in enhancing the removal of refractory contaminants is often overlooked. In this work, an efficient laccase-producing strain, Trametes hirsuta La-7, was activated to simultaneously treat BPA and antibiotics. Coexisting tetracycline, ciprofloxacin, sulfadiazine, or roxithromycin inhibited fungal cell growth, reducing laccase biosynthesis but largely increasing the formation of syringaldehyde (SYR), 4-hydroxybenzoic acid (HBA), and vanillin (VAN) through a complex regulatory network. These specialized metabolites ( i.e. , small mediators) acted as diffusible electron carriers for laccase, enabling the oxidative decomposition of the four antibiotics with high redox potentials. According to laccase-mediator-regulated radical random polymerization and decomposition, the identified intermediates of copollutants were parallelly concentrated in oligomeric coupling products and oxidative cleavage species. By inoculating logarithmic phase cell pellets in conjunction with an artificially added small mediator (SYR, HBA, or VAN), the removal efficiencies of BPA and the four antibiotics within 5 d reached 100% and 69–100% in artificial wastewater, respectively. The low and ultimately non-biotoxic intermediate products generated in the fungus-mediator systems mitigated the eco-environmental risks of the parent compounds. This work highlights the previously underappreciated role of secondary metabolism-derived small mediators in enhancing the degradation of BPA and antibiotics by a laccase-expressing fungus and is beneficial to the rational design of a robust fungus-mediator system for environmental bioremediation.
研究领域:
环境科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）