Journal of Applied Spectroscopy
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2022
·Vol. 89
·期 2
·272-280
·
IF 0.816
Wei Y.,Li L.,Ma Ch.,Wu Y.,Zhu Ch.,Gao H.,Gu J.,Xiong Y.,Li X.,Wang Z.,Wang G.,Guo S.,Chen G.
In order to directly detect the concentration of glucose in milk, carbon dots (CDs) rich in phenolic hydroxyl groups were synthesized in one step as a fluorescent probe. We presented a new glucose oxidase (GOx)-mediated strategy to detect glucose, which allowed the quantitative analysis of hydrogen peroxide (H 2 O 2 ) and glucose. Furthermore, it was possible to detect H 2 O 2 and glucose directly in complex systems such as milk. The result in milk showed that the fluorescence of the CDs was quenched by H 2 O 2 with the concentration range from 1 to 100 μM linearly, and the correlation coefficient was 0.977 with a detection limit of 0.175 μM. Similarly, a linear correlation was built between the fluorescence of the CDs and the concentration of glucose in the range from 10 to 100 μM with a correlation coefficient of 0.968 and a detection limit of 0.686 μM. The recovery rate was 97.30–101.05%, which showed high sensitivity in the detection of glucose in milk. As far as we know, this was the first time that CDs were used as a fluorescent probe to detect glucose in milk directly, which removed the step of pretreating milk and provided a supplement and extension for the detection of glucose in fluorescent spectroscopy.
研究领域:
分析化学
·
生物医学
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DOI
高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Jingjing Gu,Chunyang Chen,Tingting Liu,Yanyan Li,Xuedong Wang
Here is reported effervescent reaction-enhanced switchable hydrophilic-hydrophobic solvent-based salting-out microextraction (ERSSM) with high-performance liquid chromatography with photodiode array detection (HPLC-PDA) for the preconcentration, extraction, and trace-level determination of polybrominated diphenyl ethers (PBDEs) in milk and water. The important variables were rigorously optimized to be 150 µL of hexanoic acid as the extraction solvent, 400 µL of Na2CO3 as the alkaline source, 350 µL of H2SO4 solution as the acid source, and NaCl as the salting-out agent. The optimized conditions provided a linear range from 0.2 to 50 µg L−1 with limits of detection and quantitation from 0.026 to 0.085 µg L−1 and 0.087 to 0.28 µg L−1 for six PBDEs. The intra-day and inter-day precision values were from 2.48% to 6.75%, and the fortified recoveries for PBDEs were between 78.06% and 103.07% in water and milk samples. The developed procedure avoids the use of a traditional organic disperser and mechanical mixing allowing the determination of PBDEs outdoors by synchronous extraction method and high efficiency for the analytes. These advantages suggest a high potential for the routine monitoring of PBDEs in liquid food and environmental samples.
研究领域:
环境科学
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分析化学
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产品关联
DOI
高影响因子
Food Analytical Methods
·
2022
·Vol. 15
·期 10
·2801-2809
·
IF 3.498
Wang Xiaofei,Zhang Lu,Ye Nengsheng,Xiang Yuhong
A new metal–organic framework heterostructure composite (MOF@MOF, ZIF-8@PCN-128Y) was synthesized by an internal extension growth method and used as a sensor for the detection of tetracycline (TC). In this method, TC was enriched by virtue of the adsorption capacity of ZIF-8 and detected by PCN-128Y acting as an optical tentacle in virtue of its luminescence advantage. TC was rapidly detected with sensitivity and selectivity, and the limit of detection was 60 nM. At the same time, this method has good linearity and a wide linear range. TC in milk and beef samples was determined by this sensor, and the recovery rate was 94.2–106.3%. This work provided guidance for the construction and application of the MOF@MOF fluorescence sensor.
研究领域:
材料科学
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分析化学
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DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
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2012
·Vol. 35
·期 8
·1036-1043
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IF 2.733
Wen Zhan,Fangdi Wei,Guanhong Xu,Zheng Cai,Shuhu Du,Xuemin Zhou,Fei Li,Qin Hu
A water compatible molecularly imprinted polymers (MIPs) coated stir bar for bisphenol A(BPA) was prepared with 3,3′,5,5′-tetrabromobisphenol A as the dummy template molecule in this study. The dummy molecularly imprinted polymers coated stir bar (DMIPs-SB) showed better selectivity than the bars coated with polydimethylsiloxane or non-imprinted polymers when used to extract BPA and its three analogues. The saturated adsorption amount of the DMIPs coating was 3.0 times over that of the non-imprinted polymers coating. To achieve the optimum extraction performance, several parameters, including extraction and desorption time, pH value, adsorption temperature and stirring speed were investigated. The high-performance liquid chromatography combined with the DMIPs-SB was employed in the analysis of BPA in aqueous solution. The linear range of BPA concentration in aqueous medium was 0.0228–2.28 ng/mL with correlation coefficient of 0.9994 and the detection limit was about 6.84 × 10 −3 ng/mL based on three times ratio of signal to noise. This method was directly applied to the determination of trace BPA in milk with satisfactory results.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2014
·
IF 6.451
Hongliang Tan,Qian Li,Chanjiao Ma,Yonghai Song,Fugang Xu,Shouhui Chen,Li Wang
It is highly desirable to develop a simple and sensitive method for Hg 2+ detection because of the dangerous nature of Hg 2+ . In this work, we prepared a dual-emission fluorescent probe for Hg 2+ detection by combining two lanthanide chelates with different emission wavelengths . Green-emitting terbium (Tb 3+ ) chelates as reference signals were embedded into SiO 2 nanoparticles and red-emitting europium (Eu 3+ ) chelates as response units were covalently linked to the surface of silica shell. Upon the addition of Hg 2+ , the fluorescence of Eu 3+ chelates can be selectively quenched, while the fluorescence of Tb 3+ chelates remained unchanged. As a kind of Hg 2+ nanosensor , the dual-emission fluorescent probe exhibited excellent selectivity to Hg 2+ and high sensitivity up to 7.07 nM detection limit. The Hg 2+ levels in drinking water and milk samples were determined by using the dual-emission fluorescent probe with satisfied recovery. Additionally, our probe has a long enough fluorescence lifetime , which can avoid the interference from autofluorescence of the biological samples. We envision that the proposed probe could find great potential applications for ultrasensitive time-resolved fluorometric assays and biomedical imaging in the future.
研究领域:
分析化学
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环境科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
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2016
·
IF 3.388
Ce Shi,Xiaowei Zhang,Xingchen Zhao,Rizeng Meng,Zonghui Liu,Xiangrong Chen,Na Guo
Staphylococcus aureus ( S . aureus ) is the leading cause of a broad range of human infections and associated with food poisoning worldwide. Nisin can be considered as a natural preservative and is widely used by the food industry. However, resistance of nisin has been reported in several species of bacteria. Identification of antibacterial agents that are synergistic with nisin is a method of minimising resistance to nisin. Cinnamaldehyde (CA), a well-studied essential oil isolated from cinnamon bark, has been reported to exhibit a broad spectrum of antimicrobial activity across a wide range of microorganisms. Previous studies have demonstrated that CA could be used in the food industry due to its active antibacterial activity and its safety. In this study, the synergistic antibacterial activity in vitro of nisin and CA against 13 food-borne isolates of S. aureus and S. aureus ATCC 29213 were evaluated by a checkerboard assay. The FICI values of nisin combined with CA ranged from 0.1875 to 0.375, which demonstrated a synergistic effect. The positive interactions were verified using the challenge tests in pasteurized milk and agar diffusion assays. The LIVE/DEAD BacLight experiment and scanning electron microscope (SEM) assay were also performed to distinguish the intact and damaged both of cell walls and membranes with the compounds, the combination group displayed stronger damage both of cell wall and cell membrane than when nisin or CA was used alone. These findings indicated that the combination of nisin and CA could not only be used as a promising naturally sourced food preservative but also to reduce the problem of bacterial resistance.
研究领域:
食品科学
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微生物学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2017
·
IF 2.536
Nan Gao,Pengcheng Huang,Fangying Wu
In this study, we have developed a method for rapid, highly efficient and selective detection of melamine . The negatively charged citrate ions form an electrostatic layer on gold nanoparticles (AuNPs) and keep the NPs dispersed and stable. When citrate-capped AuNPs were further modified with Triton X-100, it stabilized the AuNPs against the conditions of high ionic strength and a broad pH range. However, the addition of melamine caused the destabilization and aggregation of NPs. This may be attributed to the interaction between melamine and the AuNPs through the ligand exchange with citrate ions on the surface of AuNPs leading Triton X-100 to be removed. As a result, the AuNPs were unstable, resulting in the aggregation. The aggregation induced a wine red-to-blue color change, and a new absorption peak around 630 nm appeared. Triton X-100-AuNPs could selectively detect melamine at the concentration as low as 5.1 nM. This probe was successfully applied to detect melamine in milk. Furthermore, paper-based quantitative detection system using this colorimetric probe was also demonstrated by integrating with a smartphone.
研究领域:
食品科学
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纳米技术
·
分析化学
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2016
·Vol. 9
·期 8
·2342-2351
·
IF 2.167
Niu Yuling,Liu Cong’an,Yang Jin,Ma Meihua,Gong Yanru,Wang Yue,Gong Bolin
Silica particles were modified with 3-methacryloxypropyl trimethoxysilane (MPS) as a carrier material. The tetracycline (TC) molecular imprinting polymer based on the surface of modified silica (MPS/SiO 2 ) was prepared by adopting the novel surface molecular imprinting technique with tetracycline as template molecule and ethylene glycol diglycidyl ether (EGDE) as crosslinker. The rebinding capacity of molecularly imprinted polymer (MIP) was evaluated according to the adsorption kinetics and the adsorption isotherm. The two distinct linear portions in Scatchard analysis indicate that two classes of binding sites existed in the imprinted polymer. The MIP obtained was used as a selective sorbent for molecularly imprinted solid-phase extraction (MISPE) prior to HPLC analysis. The results showed that good linearity was observed in the range of 150∼950 μg/L for TC, with a correlation coefficient of 0.9972. Adding level of 300∼700 μg/L, the average of spiked recoveries of TC is about 81.1 %, with a relative standard deviation (RSD) range from 3.0 to 4.4 %, and the limit of detection (LOD) reached 25 μg/L. The proposed method is easy to operate, reliable, and applicable to analyze the TC residues in milk samples.
研究领域:
分析化学
·
食品科学
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产品关联
DOI
高影响因子
ACS Omega
·
2022
·
IF 4.132
Qiaoling Yang,Bing Niu,Shuqing Gu,Jinge Ma,Chaomin Zhao,Qin Chen,Dehua Guo,Xiaojun Deng,Yongai Yu,Feng Zhang
To develop a rapid detection method for nonprotein nitrogen adulterants, this experiment sets up a set of point-scan Raman hyperspectral imaging systems to qualitatively distinguish and quantitatively and positionally analyze samples spiked with a single nonprotein nitrogen adulterant and samples spiked with a mixture of nine nonprotein nitrogen adulterants at different concentrations (5 × 10–3 to 2.000%, w/w). The results showed that for samples spiked with single nonprotein nitrogen adulterants, the number of pixels corresponding to the adulterant in the region of interest increased linearly with an increase in the analyte concentration, the average coefficient of determination (R2) was above 0.99, the minimum detection concentration of nonprotein nitrogen adulterants reached 0.010%, and the relative standard deviation (RSD) of the predicted concentration was less than 6%. For the sample spiked with a mixture of nine nonprotein nitrogen adulterants, the standard curve could be used to accurately predict the additive concentration when the additive concentration was greater than 1.200%. The detection method established in this study has good accuracy, high sensitivity, and strong stability. It provides a method for technical implementation of real-time and rapid detection of adulterants in milk powder at the port site and has good application and promotion prospects.
研究领域:
食品安全检测
·
光谱学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Veterinary Science
·
2023
·
IF 3.2
Zhang Lei,Shen Hong,Zhang Jiyou,Mao Shengyong
Our objective was to investigate the contribution of the rumen microbiome on the individual milk fat percentage (MFP) of Holstein dairy cows under the same nutritional and management conditions. From 92 early lactation dairy cows, the top 10 with the highest MFP (HF; n = 10) and the last 10 with the lowest MFP (LF; n = 10) were selected for the study. As a result, the milk trans-10, cis-12 C18:2 content was significant lower in the HF group than that in the LF group (P < 0.001). The rumen acetate to propionate ratio was significant higher in the HF group than that in the LF group (P = 0.035). According to the results of 16S rRNA gene sequencing, a minor but significant difference existed between the groups (P = 0.040). Three genera of the family Lachnospiraceae and four genera of the order Bacteroidales were identified to be the biomarkers for the LF group and HF group in the LEfSe analysis, respectively. Three microbial modules enriched by the family Lachnospiraceae were positively related to the milk trans-10, cis-12 C18:2 content (rs > 0.60, P < 0.05). According to the results of shotgun metagenome sequencing, three kinds of linoleic acid (LA) isomerase genes were present in the gene pools of the rumen microbiome. Among them, the relative abundance of Bifidobacterium LA isomerase (BBI) was higher in the HF group than that in the LF group (P = 0.007). Three metagenome-assembled genomes (MAGs) with LA isomerase genes were positively correlated to the milk trans-10, cis-12 C18:2 content (rs> 0.40, P < 0.05). Furthermore, all of these three MAGs were found to be able to produce lactate. Taken together, these results indicate that the increased relative abundance of microbial population with the trans-10 biohydrogenation pathway within the rumen microbiome contributes to the decrease of MFP via the increase of rumen trans-10, cis-12 C18:2 production. This study provides a new perspective for the development of measures for improving the milking performance of dairy cows.
研究领域:
动物科学
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微生物学
·
营养学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Fengjiao Yang,Bingyu Yang,Xinyue Gu,Minghua Li,Kezhen Qi,Ya Yan
Water-soluble AgInS 2 (AIS) quantum dots (QDs) were successfully prepared through the one-pot water phase method with thioglycolic acid (TGA) as the stabilizing agent. Because enrofloxacin (ENR) effectively quenches the fluorescence of AIS QDs, a highly-sensitive fluorescence detection method is proposed to detect ENR residues in milk. Under optimal detection conditions, there was a good linear relationship between the relative fluorescence quenching amount (Δ F / F 0 ) of AgInS 2 with ENR and ENR concentration ( C ). The detection range was 0.3125–20.00 μg/mL, r = 0.9964, and the detection limit (LOD) was 0.024 μg/mL (n = 11). The average recovery of ENR in milk ranged from 95.43 to 114.28%. The method established in this study has advantages including a high sensitivity, a low detection limit, simple operation and a low cost. The fluorescence quenching mechanism of AIS QDs with ENR was discussed and the dynamic quenching mechanism of light-induced electron transfer was proposed.
研究领域:
食品科学
·
分析化学
·
生物医学
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 15
·3006-3013
·
IF 1.915
Ye Wang,Ning Gan,Tianhua Li,Yuting Cao,Futao Hu,Yinji Chen
Herein, a facile, signal-on and homogenous fluorescence assay using novel aptamer-dsDNA antibody–quantum dot probes was designed for detecting antibiotic residues. In this assay, the fluorescent probes were fabricated by using a double-stranded DNA antibody (dsDNA Ab) covalently bonded on the surface of CdSe quantum dots. The antibody can bind double-stranded DNA (dsDNA) specifically. When dsDNA was added, the fluorescence could be efficiently quenched because the probes came into contact with each other with dsDNA as the bridge. The dsDNA was formed between an aptamer towards chloromycetin (CAP) and its complementary strand DNA (cDNA). When CAP was added into the reaction system, the aptamer reacted with CAP preferentially and the dsDNA was unwinded to single strand DNA (ssDNA) which cannot be recognized by the dsDNA antibody. Thus the probes were separated and fluorescence was recovered. Based on the scheme, a signal-on fluorescence assay for detecting antibiotics in a homogenous aqueous phase was developed. Under the optimized conditions, the assay shows a good liner range from 0.05 ng mL−1 to 100 ng mL−1 and the limit of detection (LOD) is 0.002 ng mL−1. If the analyte is changed, the assay can also be employed only by changing the corresponding aptamer and its cDNA. What's more, the probes can be reused at least 10 times with the recovery above 90%. The fluorescence assay is facile and has promising application for detecting antibiotic contaminants in biological and environmental samples.
研究领域:
生物化学
·
分析化学
·
食品安全
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DOI
高影响因子
危险品化学品经营许可证（带存储）