Nature Communications
·
2024
·Vol. 15
·期 1
·1-12
·
IF 14.7
Cui Zhanding,Liu Jinlong,Xie Chong,Wang Tao,Sun Pu,Wang Jinlong,Li Jiaoyang,Li Guoxiu,Qiu Jicheng,Zhang Ying,Li Dengliang,Sun Ying,Yin Juanbin,Li Kun,Zhao Zhixun,Yuan Hong,Bai Xingwen,Ma Xueqing,Li Pinghua,Fu Yuanfang,Bao Huifang,Li Dong,Zhang Qiang,Liu Zaixin,Cao Yimei,Zhang Jing,Lu Zengjun
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) poses a major threat to the global swine industry, yet effective prevention and control measures remain elusive. This study unveils Nitazoxanide (NTZ) as a potent inhibitor of PRRSV both in vitro and in vivo. Through High-Throughput Screening techniques, 16 potential anti-PRRSV compounds are identified from a library comprising FDA-approved and pharmacopeial drugs. We show that NTZ displays strong efficacy in reducing PRRSV proliferation and transmission in a swine model, alleviating viremia and lung damage. Additionally, Tizoxanide (TIZ), the primary metabolite of NTZ, has been identified as a facilitator of NMRAL1 dimerization. This finding potentially sheds light on the underlying mechanism contributing to TIZ’s role in augmenting the sensitivity of the IFN-β pathway. These results indicate the promising potential of NTZ as a repurposed therapeutic agent for Porcine Reproductive and Respiratory Syndrome (PRRS). Additionally, they provide valuable insights into the antiviral mechanisms underlying NTZ’s effectiveness.
研究领域:
兽医学
·
分子生物学
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药理学
·
病毒学
·
治疗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2014
·
IF 1.019
Yu-Xiang Yang,Chun-Long Mu,Jing-Fei Zhang,Wei-Yun Zhu
A rapid high-performance liquid chromatographic method for the determination of nine biogenic amines in digesta sample from pig cecum and colon was developed using a simple direct derivatization with dansyl chloride. After precipitation with trichloroacetic acid and extraction by n-hexane, the amines were separated on a C18 column using a water–acetonitrile gradient elution program. The calibration curve for each amine was linear over the range of 50 to 250 µmol/L, and the relative standard deviation for intra-assay precision was below 0.5%. The recovery ranged from 86.79% to 117.62% while the limit of detection was 0.1 µmol/L for the amines except for spermine with a value of 0.03 µmol/L. This procedure was successfully applied to identify and quantify biogenic amines in the hindgut digesta of the pig.
研究领域:
分析化学
·
动物营养学
✓ 已验证
DOI
高影响因子
Biomass Conversion and Biorefinery
·
2024
·
IF 3.5
Ouyang Yue,Sun Wei,Tian Longjin,Liu Ying,Fan Guozhi,Pan Cheng,Cheng Qunpeng
In this study, the performance of biochar (BC)-supported cobalt (Co) and copper (Cu) (CoCu@BC) on tetracycline (TC) adsorption in aqueous solution and piggery wastewater was investigated. Scanning electron microscopy (SEM), Brunauer–Emmett–Teller (BET) surface area, Fourier-transform infrared (FTIR) spectroscopy, and X-ray diffraction (XRD) were used to characterize the CoCu@BC adsorbent. The influence of initial pH, TC concentration, adsorbent dosage, and co-existing ions on TC adsorption performance was studied. Meanwhile, the adsorption kinetics, isotherm, and diffusion models were studied. The results showed that the CoCu@BC was characteristic of 142.1745 m 2 ·g −1 (BET surface area), 0.035987 cm 3 ·g −1 (microspore volume), and 3.74 (pH PZC ). The adsorption of TC on CoCu@BC well fitted with the pseudo-second-order model and the Freundlich isotherm. The maximum adsorption capacity of CoCu@BC was 621.118 mg/g, which was less influenced by pH while greatly inhibited by NO 2 − and SO 4 2− . Finally, the removal of TC (94.5%), NH 4 + -N (27%), and COD (18%) by CoCu@BC was carried out in piggery wastewater, indicating its superior performance in practice application.
研究领域:
环境科学
·
污染控制
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Shengqi Jiang,Huiyu Han,Madina Aitmagambetova,Aidana Rakhimova,Lin Li,Rong Bai,Chunjie Yang,Xiang Ye,Siying Wang,Jing Xi,Wu Ding
This study aimed to investigate the effects of oligosaccharides (xylooligosaccharides (XOS), fructooligosaccharides (FOS), galactooligosaccharides (GOS), and isomaltooligosaccharides (IOS)) with different structures and molecular weights on the glycosylation modification of pigskin collagen (CO). Glycosylated CO was prepared via the Maillard reaction, and its molecular structure, physicochemical, and functional properties were analyzed using various analytical techniques. Results indicated increased glycosylation significantly altered the α-helix and random coil content, intrinsic fluorescence intensity, particle size, and ζ-potential of CO ( P < 0.05). These modifications enhanced protein-protein and protein-water interactions, improving solubility, thermal stability, rheological properties, and creating a more uniform gel network structure. XOS, composed of the smaller molecular weight five-carbon aldose unit (xylose), exhibited the most significant effects, including enhanced thermal stability, water retention, and gel strength. However, the Maillard reaction-induced glycation browning caused a slight negative impact on the color of CO gels. In conclusion, oligosaccharide glycosylation significantly enhances the functional properties of pigskin CO, providing a promising strategy for its high-value utilization.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Fangqu Wang,Zipeng Xu,Lin Chen,Ziyan Qiao,Yayun Hu,Xiaojing Fan,Yaping Liu,Zhuangli Kang,Feng Huang,Minyi Han,Huijuan Yang,Xianchao Feng
In order to better showcase the freshness of fresh meat, merchants usually place absorbent pads under tray packaging for preservation. However, the current absorbent pads can only absorb a small amount of blood water and cannot effectively inhibit the growth and reproduction of microorganisms in tray packaging. Herein, a super absorbent aerogel pad for preserving fresh meat was prepared through the Schiff base reaction. Benefiting from the superhydrophilicity and porous structure of aerogels, the prepared G3D1BC0.5 (Gelatin: Dialdehyde starch = 3:1, Bacterial cellulose : 0.5%) aerogel not only possessed excellent adsorption performance but also rapidly regain their shape under 70% compression strain. The G3D1BC0.5 aerogel incorporating curcumin (Cur) showed the good antibacterial ability where the survival rate of Listeria monocytogenes , Staphylococcus aureus , Escherichia coli and Salmonella typhimurium significantly decreased to lower than 45% with the increase of Cur content ( P < 0.05). Furthermore, the prepared aerogels had the excellent preservation effect on fresh meat during storage. The G3D1BC0.5-Cur0.7 aerogels can extend the shelf-life of fresh pork to 12 days by inhibiting microbial growth , protein oxidation, and lipid oxidation. Therefore, the prepared aerogel in the present study had great potential for preservation of fresh pork.
研究领域:
食品科学
·
材料科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Veterinary Science
·
2024
·
IF 2.6
Xu Changzhi,Yang Xianshu,Sui Heming,Tong Xu,Zhang Dandan,Zheng Xianrui,Jiao Jun,Wang Chonglong,Cao Zubing,Zhang Yunhai
According to previous studies, the quality and fertilization rate of fresh sperm from boars of different ages were significantly different. However, the difference of freeze–thaw sperm quality and fertility in boars of different ages is unclear. In this study, boars of a Chinese native breed were assigned into two groups. Each group consisted of five boars aged aged either 2–3 years (young boars = YB) or 5–6 years (aging boars = AB) A total of 60 ejaculates for each group were collected and cryopreserved. Semen quality and in vitro fertility of post-thaw sperm was evaluated. The results showed that the concentration and motility of fresh sperm collected from AB were similar to YB, but their semen volume was higher than that in YB (p < 0.05). Frozen–thawed sperm of AB had lower viability than YB, and higher abnormal rate and reactive oxygen species (ROS) levels of YB (p < 0.05). There was no effect of the age on post-thaw sperm motility and time survival. Functional assessments indicated that increasing age markedly compromises the integrity of the sperm plasma membrane and acrosome, as well as mitochondrial functionality post-thaw, albeit without affecting DNA integrity. Furthermore, increasing age of boars reduces the ability of sperm to bind to the oocyte zona pellucida after thawing, delaying the time of the first embryo cleavage after fertilization. Finally, the early developmental efficiency of in vitro fertilized embryos progressing from 4-cell to blastocyst derived from post-thaw sperm in AB significantly decreased compared to those from YB (p < 0.05). Taken together, these results suggest that increasing age in boars impairs the quality and in vitro fertility of frozen thawed sperm.
研究领域:
动物科学
·
生殖生物学
·
兽医学
✓ 已验证
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Mengyuan Wang,Lin Chen,Bofu Han,Renzheng Wang,Yaping Liu,Xiaojing Fan,Xin Lv,Feng Huang,Minyi Han,Zhuangli Kang,Xianchao Feng,Igor Mazurenko
Effects of sweetener neomethyl hesperidin dihydrochalcone (NHDC) on the gelation properties of myofibrillar protein (MP) at 0.2 M and 0.6 M NaCl were investigated. The results showed that addition of NHDC did not obviously change the cooking loss and strength of MP gels at 0.2 M NaCl. Whilst at 0.6 M NaCl, addition of high doses of NHDC (50–100 µM/g) remarkably increased cooking loss and decreased strength of MP gels. The expanded MP enhanced the interactions between NHDC and MP, leading to aggregation of MP as evidenced by the increasing particle size and the reducing solubility. The MP aggregates impeded hydrophobic interactions and disulfide bonds crosslinking in the gelation process, thereby damaged the gelation properties of MP at 0.6 M NaCl. This was supported by the poor network of MP gels observed by SEM. In the end, molecular docking elucidated the main types of interaction at molecular level, including hydrogen bonding and hydrophobicity. The results would provide guidance for NHDC as a potential sweetener in meat products.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Guangzhi Wang,Yuchen Guo,Yang Yu,Yan Shi,Yuxiang Ying,Hong Men
Pork freshness is crucial for flavour, nutrition and consumer health. The current colorimetric sensor array (CSA) detection systems face challenges related to high sensor development costs, low recognition accuracy and limitations in the platform use. Herein, we developed a CSA and ColorNet framework to detect pork freshness. The 53-point CSA was designed by selecting sensitised pH indicators and aldehyde/ketone indicators. To optimize the sensor, the Euclidean distance method was used to identify 24 array points with dyes that exhibited more sensitive responses. The ColorNet captured the color information of pork freshness, allowing real-time detection with a 99.5 % accuracy. For practical deployment and mobile applications, a refined 12-point CSA was developed using gradient activation mapping, maintaining a 99 % recognition rate, which is comparable with the 24-point CSA. The proposed CSA and model ensure consumer health and safety, providing strong technical support for quality monitoring and control in the pork industry.
研究领域:
食品科学
·
人工智能
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biochar
·
2021
·Vol. 3
·期 3
·391-405
·
IF 0
Li Chao,Sun Yifan,Zhang Lijun,Wang Changxu,Zhang Shu,Li Qiaoling,Xu Leilei,Hu Xun
Biomass and pig manure have distinct compositions and the co-pyrolysis of them has gained much attention. However, the influence of volatiles interaction on the properties of the char was still unclear. In this study, lignin was selected as the model component of biomass with pig manure for co-pyrolysis at 600 °C. The results indicate that volatiles from co-pyrolysis promoted re-condensation reaction, resulting in the higher char yield (48.0% in co-pyrolysis versus 31.0% in pyrolysis of single manure) and the formation of more aromatics in bio-oil. The co-pyrolysis also facilitated the dehydrogenation and dehydration reactions, which accounted for the elimination of oxygen and nitrogen contents and thus a higher carbon content (64.7% in the co-pyrolysis versus the averaged value of 46.4% from the pyrolysis of single feedstock), higher crystallinity and thermal stability of the char. The in-situ diffuse reflection infrared Fourier transform spectroscopy (DRIFTS) characterization results demonstrated that the functionalities abundances of char with temperature was influenced by volatiles interaction via accelerating the carbonization reaction. In addition, the high heating value (HHV) of char was obviously improved by cross-interaction of volatiles during co-pyrolysis (24.4 MJ/Kg in co-pyrolysis versus averaged value of 15.1 MJ/Kg from single pyrolysis), implying that the co-pyrolysis enhanced the energy density of the resulting char. Graphic abstract
研究领域:
能源与环境科学
·
化学工程
✓ 已验证
DOI
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Mengxiang Wang,Jinxing Song,Yanze Li,Junru Sun,Lei Zhou,Zhuoya Sun,Angke Zhang,Guoqing Zhuang,Pingan Xia,Gaiping Zhang,Yanan Wu
African swine fever has caused huge losses to the global pig industry. In the absence of effective vaccines, reliable detection methods are crucial. The p30 protein of ASFV is often used as a target for detection due to its high antigenicity in the early stage of virus replication. In this study, we modified the p30 protein using magnetic nanoparticles to increase its specific surface area and suspension in solution. Based on this modified p30 protein, we optimized the experimental steps to determine the best reaction conditions and then established a double probe competitive ELISA method. The established detection method can produce results within 45 min, with repeatability between and within batches <15 %, and it does not react with other pathogens, which demonstrates high specificity. In conclusion, we modified the ASFV p30 protein with magnetic nanoparticles and established a rapid, ultra-sensitive, and convenient detection method for ASF based on the modified p30. This also provides a new reference for the rapid detection of other veterinary diseases.
研究领域:
兽医学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Bio
·
2025
·
IF 10.2
Xiaobo Yu,Jingli Ding,Yingjie He,Shunbo Wei,Xing Chen,Qiujie Luo,Yuqing Zhang,Chen Qian,Jiahui Wang,Mengjie Hu,Xiang Zhang,Cuifen Lu,Jinping Liu,Jianliang Zhou
Valvular heart disease (VHD) poses a thorny problem in cardiovascular diseases. The most effective treatment for VHD is heart valve replacement. Biological heart valve (BHV) is more favored than mechanical heart valve due to the maturity of transcatheter heart valve replacement (THVR) and the absence of the need for lifelong anticoagulant use. However, traditional commercial BHV suffers degeneration within 10 to 15 years because of calcification caused by the cross-linking reagent, glutaraldehyde. Considering the remarkable properties of POSS, PEG, and the star-like eight-arm structure, we fabricated POSS-PEG-PP, which is a decellularized porcine pericardium (DPP) crosslinked by a star-like eight-arm cross-linker octafunctionalized POSS of benzaldehyde-terminated polyethylene glycol (POSS-PEG-CHO) based on the Schiff's base reaction. POSS-PEG-PP exhibits more intense fiber arrangement and better mechanical properties than GLUT-PP (glutaraldehyde crosslinked DPP). The results also show that the cytocompatibility, endothelialization, and hemocompatibility of POSS-PEG-PP are outstanding in vitro. Subsequently, in vivo assessments demonstrate that POSS-PEG-PP has anti-inflammatory and anti-calcification abilities. Furthermore, RNA sequencing analysis of subcutaneous implants suggests that the intervention of AMPK and IL-17 signaling pathways plays an important role in the inflammatory and immune responses regulation of POSS-PEG-PP. Therefore, POSS-PEG-PP is an excellent substitute material for BHVs and is expected to be clinically transformed.
研究领域:
心血管疾病
·
生物材料科学
·
组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CyTA-Journal of Food
·
2025
·
IF 1.7
Ziyi Hu,Liusha Kuang,Lin Li,Enqi He,Lei Zhou,Aihua Lou,Yan Liu,Jie Luo,Wei Quan,Qingwu Shen
The correlations between meat quality, dominant microbiota, and key volatile compounds during chill storage were investigated by HS-SPME-GC-MS, Illumina sequencing and correlation analysis. The results indicated that lipid and protein oxidation of pork belly increased significantly on day 4, biogenic amine index reaching 344.07 mg/kg on day 6, the total viable count reached 7.98 log10 (CFU/g) at day 8, with Macrococcus caseolyticus (54.60%), unclassified g Acinetobacter (23.70%) and Acinetobacter bereziniae (7.75%) identified as dominant bacteria. HS-SPME-GC-MS identified 66 compounds with tetramethyl-pyrazine, acetoin, 1-hexadecanol, and hexadecanoic acid were found to be related to meat quality and influenced by microbial activity, serving as indicators of pork belly freshness. However, due to the complexity of microbial communities in aquatic products, further research is needed to explore the relationship between changes in microbial communities and the volatile organic compounds (VOCs). These findings provide valuable insight into key factors contributing to spoilage in Ningxiang pork.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）