ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Yong’an Tang,Jun Hu,Ahmed H. Elmenoufy,Xiangliang Yang
Photodynamic therapy (PDT) for deep-seated tumor is largely impeded by the limited penetration depth of excitation light in tissue. X-ray is considered as an ideal energy source to activate photosensitizers (PSs) located deep within the body with the assistance of scintillating nanoparticles (ScNPs). However, the efficacy under this concept is not satisfying due to the low scintillating luminescence and weak energy transfer from ScNPs to PSs. Here, mesoporous LaF3:Tb ScNPs were successfully synthesized by a facile hydrothermal process to act as PS carriers and X-ray energy transducers, owing to their good ionizing radiation stopping power and high luminescence efficiency. The formation mechanism of porous structure was elucidated in detail with classical crystallization theory. After a systematic investigation, LaF3:Tb ScNPs with optimized scintillating luminescence were obtained for loading Rose Bengal (RB) to establish an efficient FRET system, owing to their excellent spectral match. The FRET efficiency between ScNPs and RB was calculated to be as high as 85%. Under irradiation, enhanced 1O2 generation induced by LaF3:Tb-RB nanocomposites via FRET process was detected. This LaF3:Tb-RB FRET system shows great potential to be applied in X-ray stimulated PDT for deep-seated tumors in the future.
研究领域:
肿瘤学
·
纳米医学
·
光动力治疗
·
分子影像
·
放射化学
·
生物材料
·
转化医学
✓ 已验证
DOI
高影响因子
PSYCHOPHARMACOLOGY
·
2022
·Vol. 239
·期 11
·3579-3593
·
IF 4.415
Mou Chen-Ye,Xie Yan-Fei,Wei Jia-Xin,Wang Qi-Yao,Le Jing-Yang,Bao Yong-Jie,Zhang Pan-Pan,Mao Yue-Chun,Huang Xing-Han,Pan Han-Bo,Naman C. Benjamin,Liu Lin,Liang Hong-Ze,Wu Xiang,Xu Jia,Cui Wei
Rationale
Tau hyperphosphorylation and aggregation is considered as a main pathological mechanism underlying Alzheimer’s disease (AD). Rose Bengal (RB) is a synthetic dye used for disease diagnosis, which was reported to inhibit tau toxicity via inhibiting tau aggregation in Drosophila . However, it was unknown if RB could produce anti-AD effects in rodents. Objectives The research aimed to investigate if and how RB could prevent β-amyloid (Aβ) oligomers-induced tau hyperphosphorylation in rodents. Methods and results RB was tested in vitro (0.3–1 μM) and prevented Aβ oligomers-induced tau hyperphosphorylation in PC12 cells. Moreover, RB (10–30 mg/kg, i.p. ) effectively attenuated cognitive impairments induced by Aβ oligomers in mice. Western blotting analysis demonstrated that RB significantly increased the expression of pSer473-Akt, pSer9-glycogen synthase kinase-3β (GSK3β) and reduced the expression of cyclin-dependent kinase 5 (CDK5) both in vitro and in vivo. Molecular docking analysis suggested that RB might directly interact with GSK3β and CDK5 by acting on ATP binding sites. Gene Ontology enrichment analysis indicated that RB might act on protein phosphorylation pathways to inhibit tau hyperphosphorylation. Conclusions RB was shown to inhibit tau neurotoxicity at least partially via inhibiting the activity of GSK3β and CDK5, which is a novel neuroprotective mechanism besides the inhibition of tau aggregation. As tau hyperphosphorylation is an important target for AD therapy, this study also provided support for investigating the drug repurposing of RB as an anti-AD drug candidate.
研究领域:
神经科学
·
药理学
·
分子生物学
·
病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Ming Huang,Yingfeng Wang,Yuhan Zhu,Maoli Yin,Zhipeng Ma,Xiaojuan Li
To address the challenge of antibiotic resistance and cross-infection from microorganisms, chitosan (CS) was covalently bound with rose bengal (RB) and blended with polyethylene oxide (PEO) to prepare nanofiber membranes with good antibacterial properties. Morphological and structural characteristics confirmed successful integration of RB-modified CS (CS-RB) without compromising the fiber morphology or stability of PEO/CS-RB nanofiber membranes. FT-IR and TG analyses were employed to characterize the chemical structure and thermal stability of PEO-based membranes. With visible light irradiation, PEO/CS-RB nanofiber membranes efficiently generated reactive oxygen species (ROS), enabling rapid inactivation of all Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Candida albicans (C. albicans) within 30 min. The PEO-based membranes exhibited well-balanced hydrophilicity and water stability, along with low hemolytic activity, indicating good biocompatibility. This strategy creates PEO-based membrane materials that exhibit good biocompatibility, hydrophilicity, and photodynamic antibacterial properties, making them suitable for use in the biomedical and healthcare fields.
研究领域:
材料科学
·
生物医学工程
·
抗菌技术
·
纳米技术
·
高分子科学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 19
·8297-8303
·
IF 1.821
Kaisong Yuan,Jianzhuang Wang,Haiyun Zhai,Zuanguang Chen,Lu Huang,Zihao Su
A method employing molecularly imprinted solid-phase extraction (MISPE) coupled with capillary electrophoresis laser-induced fluorescence detection (CE-LIF) was developed for the determination of rose bengal in brown sugar. The rose bengal imprinted monolithic column was prepared by in situ polymerization, utilizing rose bengal as a template, methacrylic acid as a functional monomer and ethylene dimethacrylate as a cross-linker. The capacity and specificity of this column were evaluated by CE-LIF, and the morphology was characterized by scanning electron microscopy. The recoveries ranged from 89.7% to 90.3% (relative standard deviation 3.6–4.5%, n = 5). The capacities of the molecularly imprinted polymer and non-imprinted polymer columns were 1.314 μg mg−1 and 0.531 μg mg−1, respectively. The detection limit (S/N = 3) was 3 ng mL−1. The enrichment factor was higher than 63-fold and the MISPE column managed to remove rose bengal from the matrix of brown sugar effectively. This method can be applied to sensitively and effectively determine rose bengal in brown sugar.
研究领域:
分析化学
·
食品分析
·
分子印迹技术
·
分离科学
·
食品安全
✓ 已验证
DOI
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2020
·Vol. 20
·期 12
·7558-7568
·
IF 1.134
Chi Fenping,Chen Pengpeng,Mao Changjie
Rose Bengal (RB) was used as a functional pigment and poly dimethyl diallyl ammonium chloride was used as a coupling agent to modify Graphene Oxide (GO) in order to enhance the light absorption and ROS generation of GO. GO, RB and the obtained RB-PDDA-GO were characterized by scanning
electron microscopy, transmission electron microscopy, Fourier transform infrared spectrometry, thermogravimetric analysis, Raman spectroscopy, UV-visible spectrophotometry, and X-ray photoelectron spectroscopy. The oxidation of hydroquinone to p-benzoquinone was used to evaluate the oxidation
ability. Three kinds of reactive oxygen species (O2
·-, 1O2 and ·OH) produced by the materials under light irradiation were detected by the ESR method using TEMP (2,2,6,6-tetramethyl-4-piperidine) and DMPO (5,5-dimethyl-1-pyrroline-N-oxide)
as capture agents. The results showed that RB-PDDA-GO produced more ROS under light than GO. Antibacterial experiments were carried out with E. coli as the target strain to detect the actual utility of ROS produced by the materials. The results showed that RB-PDDA-GO had a significant
sterilization effect.
研究领域:
纳米技术
·
材料科学
·
环境科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Water Process Engineering
·
2022
·
IF 7.34
Meudjeu Tognia,Guoqing Feng,Zonglin Pan,Xinfei Fan,Chengwen Song,Tonghua Wang
A novel application of three metal oxide catalysts CuO x , Fe 2 O 3 , and CoO onto coal-based carbon membrane (CM) in the enhancement of electrochemical removal of high concentration organic pollutants is presented. Their morphology, structure, and elemental composition were characterized. Analysis of their performance in the treatment of highly concentrated Rhodamine B (RhB), Rose Bengal, and phenol pollutants with respective concentrations of 600 mg/l, 637 mg/l, and 50 mg/l presented excellent removal efficiency (all >97 %). A study of the degradation mechanism of pollutants at the membrane surface was approached through multi-physics modeling and simulation of the reaction kinetics of complex indirect RhB oxidation chemistry occurring at the copper oxide membrane surface which involved oxidation of resistant intermediates. The results gave a degradation efficiency of 99.82 % after 60 s of reaction at the CM-CuO x surface which is very close to the experimental results. An understanding of the pollutants concentration distribution across the electrocatalytic filtration system was achieved through simulation of the transport, diffusion, and convection of degraded products through the membrane walls. No fouling was observed for RhB treatment and a new insight into the interactions between pollutants molecules and reactive membrane surfaces was brought by the mean of Monte-Carlo simulations of their adsorption mechanisms. Finally, a comparison with other Electrochemical Advanced Oxidation Processes (EAOPs), revealed that the membranes fabricated in this work possessed significant advantages both in terms of permeability and removal efficiency.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Advanced Science
·
2023
·Vol. 10
·期 6
·2206009
·
IF 15.1
Shanshan Zhang,Shujun Xia,Liang Chen,Yu Chen,Jianqiao Zhou
Covalent organic frameworks (COFs) have attracted increasing attention for biomedical applications. COFs-based nanosensitizers with uniform nanoscale morphology and tumor-specific curative effects are in high demand; however, their synthesis is yet challenging. In this study, distinct COF nanobowls are synthesized in a controlled manner and engineered as activatable nanosensitizers with tumor-specific sonodynamic activity. High crystallinity ensures an ordered porous structure of COF nanobowls for the efficient loading of the small-molecule sonosensitizer rose bengal (RB). To circumvent non-specific damage to normal tissues, the sonosensitization effect is specifically inhibited by the in situ growth of manganese oxide (MnO x ) on RB-loaded COFs. Upon reaction with tumor-overexpressed glutathione (GSH), the “gatekeeper” MnO x is rapidly decomposed to recover the reactive oxygen species (ROS) generation capability of the COF nanosensitizers under ultrasound irradiation. Increased intracellular ROS stress and GSH consumption concomitantly induce ferroptosis to improve sonodynamic efficacy. Additionally, the unconventional bowl-shaped morphology renders the nanosensitizers with enhanced tumor accumulation and retention. The combination of tumor-specific sonodynamic therapy and ferroptosis achieves high efficacy in killing cancer cells and inhibiting tumor growth. This study paves the way for the development of COF nanosensitizers with unconventional morphologies for biomedicine, offering a paradigm to realize activatable and ferroptosis-augmented sonodynamic tumor therapy.
研究领域:
生物医学工程
·
纳米技术
·
癌症治疗
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Chengcheng Li,Fengming Lin,Wei Sun,Fu-Gen Wu,Hang Yang,Roujing Lv,Ya-Xuan Zhu,Hao-Ran Jia,Chu Wang,Ge Gao,Zhan Chen
It is of great value to develop new antibacterial photodynamic therapy (PDT) strategies to improve antibacterial PDT efficacy of noncationic photosensitizers without introducing cytotoxicity, which is a great challenge for current leading efforts on antimicrobial PDT based on cell surface engineering. In this research, the hydrophobic and anionic photosensitizer rose bengal (RB) was chemically conjugated with bacterial exopolysaccharide (EPS) to generate an amphiphilic and negatively charged compound EPS-RB that could self-assemble into nanoparticles (NPs) in solution. These EPS-RB NPs possessed an increased singlet oxygen generation property in solution. As a result, EPS-RB exhibited improved photoinactivation for both Gram-negative and Gram-positive bacteria, leading to a record low RB working concentration, 8 μM or 500 nM for Escherichia coli or Staphylococcus aureus, respectively. Upon light irradiation, more EPS-RB bound to the cell surface and penetrated into bacteria than RB, with EPS-RB staying around the cell surface of the most irradiated E. coli while entering all irradiated S. aureus. Both scanning electron microscopy and fluorescence confocal imaging results show that the cell membrane of E. coli was damaged heavily but not S. aureus. All of these observations indicate that both the enhanced singlet oxygen production of EPS-RB NPs in solution and their consequently increased membrane binding and cellular penetration into the bacteria through the damaged cell membrane contribute to their significantly improved bacterial photoinactivation efficiency. In addition, EPS-RB has low cytotoxicity and negligible hemolytic activity, showing great biocompatibility. Therefore, the construction of EPS-RB provides a new strategy for the PDT effectiveness improvement of the separated cell/sensitizer systems and thus the design of next-generation antimicrobial agents.
研究领域:
药剂学
·
微生物学
·
材料科学
·
治疗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Meng Ding,Wei Zhao,Xu Zhang,Lingjie Song,Shifang Luan
Bacterial biofilm is notorious for causing chronic infections, whose antibiotic treatment is bringing about severe multidrug resistance and environmental contamination. Stimuli-responsive nanocarriers have become encouraging materials to combat biofilm infections with high efficiency and low side effect. Herein, a charge-switchable and pH-responsive nanocomplex is fabricated via a facile aqueous one-pot zeolitic imidazolate framework-8 (ZIF-8) encapsulation of proteinase K (PK) and photosensitizer Rose Bengal (RB), for enzymatic and photodynamic therapies (PDT) against biofilm infections. Once encountering in acidic microenvironment, the surface charge of nanocomplex can switch self-adaptively from negative to positive, hence remarkably facilitating the biofilm penetration of nanocomplex. After acid-induced decomposition of nanocomplex, the released PK degrades biofilm matrix and loosens its structure, promoting diffusion of RB inside the biofilm. Afterwards, upon visible light illumination, the RB generates highly reactive oxygen species (ROS), which can readily and efficiently kill the remained bacteria even in the biofilm core. The charge-assisted penetration makes PK and RB fully functional, resulting in a cooperative effect concerning high biofilm eradication capacity, as testified by biofilm models both in vitro and in vivo . The green synthesis and good therapeutic performance of the nanocomplex manifests its considerable potential as a nontoxic and effective platform for biofilm treatment.
研究领域:
纳米技术
·
生物医学工程
·
药学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Cijun Shuai,Jie Zeng,Zhaoxiong Zhang,YouWen Yang,MingLi Yang,Hengyun Ye,Pan He,Jun Zan,Jun Zhang
Photosensitizer-mediated photodynamic therapy (PDT) enables wireless tumor inactivation by releasing cytotoxic reactive oxygen species (ROS). Nevertheless, the activation of conventional photosensitizers typically depends on visible light with limited tissue penetration, thereby restricting their therapeutic efficacy in deep-seated tumors. To overcome this limitation, an upconversion implant was engineered to function as a flexible internal light-emitting device by converting deeply penetrating near-infrared-I (NIR-I) light into visible light. Specifically, a composite PDT nanosystem was constructed by linking NaYF 4 :Yb,Er upconversion nanoparticles with the rose bengal photosensitizer—whose emission and absorption spectra are well-matched—using polyvinylpyrrolidone (PVP) as a molecular bridge. This PDT nanosystem was subsequently incorporated into poly-L-lactic acid (PLLA) scaffolds fabricated via selective laser sintering. Under 980 nm laser irradiation, the upconversion implant was capable of wirelessly emitting upconverted visible light through more than 2 cm of an in vitro tissue model and generating abundant ROS via the photodynamic effect. In vitro cell experiments demonstrated that the system effectively eliminated tumor cells by damaging the cell membrane and cellular genetic material. These results suggest that the scaffolds possess significant potential as anti-tumor tissue implants and offer a promising avenue for the development of innovative tumor treatment strategies.
研究领域:
生物医学工程
·
纳米技术
·
肿瘤学
·
光医学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
San-Jun Shi,Rong-Fu Huang,Zi-Yan Fan
Development of rapid and sensitive methods for screening of large number of chemical pollutants with potential genotoxicity is in urgent demands. In this work, we reported a chemical genotoxicity sensor based on the lateral flow strip (LFS) quantification of the 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo). The proposed sensing strategy introduced human 8-oxoguanine DNA glycosylase (hOGG 1) and human apyrimidinic endonuclease (APE 1) to convert 8-oxodGuo to strand break. Then, the DNA conjugated gold nanoparticles (AuNPs) complexes could not be captured on test line (T-line), showing visible red color fading. Additionally, the mean grey value of the optical density in the T-line zone was analyzed by using a mobile phone camera and Image J software to quantify 8-oxodGuo. Under the optimized experimental conditions, the LFS DNA biosensor could detect the lowest concentration of 8-oxodGuo down to 0.2 pmol. To demonstrate its universal applications, in vitro generation of 8-oxodGuo by Rose Bengal under irradiation and Fe 2+ -mediated Fenton reagent were successfully measured. The detection limit of oxidative DNA damage induced by Rose Bengal or Fenton reagent could be down to 0.01 μM or 1 nM Fe 2+ /4 nM H 2 O 2 , respectively. The 10-fold higher sensitivity for Fenton reagent was attributed to much more severe damage than Rose Bengal, producing both DNA strand break and nucleobases conversion. The results demonstrated that the LFS DNA biosensor could detect oxidative DNA damage for the first time, showing its potential application for assessment of the genotoxicity of abundant chemical pollutants.
研究领域:
分析化学
·
环境科学
·
生物技术
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2020
·
IF 4.389
Kai Chen,Cong Chang,Zuhao Liu,Yimin Zhou,Qingni Xu,Chaohua Li,Zhijun Huang,Haixing Xu,Peihu Xu,Bo Lu
In this work, a pH-responsive and tumor targeted multifunctional drug delivery system (RB-DOX@HMSNs-N = C-HA) was designed to realize chemo-photodynamic combination therapy. Hollow mesoporous silica nanoparticles (HMSNs) was served as the host material to encapsulate doxorubicin (DOX) and photosensitizer rose bengal (RB). Hyaluronic acid (HA) was modified on the surface of HMSNs via pH-sensitive Schiff base bonds as gatekeeper as well as targeted agent. Characterization results indicated the successful preparation of HMSNs-N = C-HA with appropriate diameter of 170 nm around and the nanocarriers displayed superior drug loading capacity (15.30 % for DOX and 12.78 % for RB). Notably, the results of in vitro drug release experiments confirmed that the system possessed good pH-sensitivity, which made it possible to release cargoes in slight acid tumor micro-environments. Significantly, the in vitro cell uptake and cytotoxicity assay results fully proved that RB-DOX@HMSNs-N = C-HA could precisely target murine mammary carcinoma (4T1) cells and effectively inhibit tumor cells viability with chemo-photodynamic synergistic therapy. Overall, our work (RB-DOX@HMSNs-N = C-HA) provides an efficient approach for the development of chemo-photodynamic combination therapy.
研究领域:
纳米技术
·
药学
·
癌症治疗
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）