ANALYTICAL CHEMISTRY
·
2015
·
IF 5.636
Yan-Ran Li,Qian Liu,Zhangyong Hong,He-Fang Wang
For the widely used “off-on” fluorescence (or phosphorescence) resonance energy transfer (FRET or PRET) system, the separation of donors and acceptors species was vital for enhancing the sensitivity. To date, separation of free donors from FRET/PRET inhibition systems was somewhat not convenient, whereas separation of the target-induced far-between acceptors has hardly been reported yet. We presented here a novel magnetic separation-assistant fluorescence resonance energy transfer (MS-FRET) inhibition strategy for highly sensitive detection of nucleolin using Cy5.5-AS1411 as the donor and Fe3O4-polypyrrole core–shell (Fe3O4@PPY) nanoparticles as the NIR quenching acceptor. Due to hydrophobic interaction and π–π stacking of AS1411 and PPY, Cy5.5-AS1411 was bound onto the surface of Fe3O4@PPY, resulting in 90% of fluorescence quenching of Cy5.5-AS1411. Owing to the much stronger specific interaction of AS1411 and nucleolin, the presence of nucleolin could take Cy5.5-AS1411 apart from Fe3O4@PPY and restore the fluorescence of Cy5.5-AS1411. The superparamagnetism of Fe3O4@PPY enabled all separations and fluorescence measurements complete in the same quartz cell, and thus allowed the convenient but accurate comparison of the sensitivity and fluorescence recovery in the cases of separation or nonseparation. Compared to nonseparation FRET inhibition, the separation of free Cy5.5-AS1411 from Cy5.5-AS1411-Fe3O4@PPY solution (the first magnetic separation, MS-1) had as high as 25-fold enhancement of the sensitivity, whereas further separation of the nucleolin-inducing far-between Fe3O4@PPY from the FRET inhibition solution (the second magnetic separation, MS-2) could further enhance the sensitivity to 35-fold. Finally, the MS-FRET inhibition assay displayed the linear range of 0.625–27.5 μg L–1 (8.1–359 pM) and detection limit of 0.04 μg L–1 (0.05 pM) of nucleolin. The fluorescence intensity recovery (the percentage ratio of the final restoring fluorescence intensity to the quenched fluorescence intensity of Cy5.5-AS1411 solution by 0.09 g L–1 Fe3O4@PPY) was enhanced from 36% (for nonseparation) to 56% (for two magnetic separations). This is the first accurate evaluation for the effect of separating donor/acceptor species on the FRET inhibition assay.
研究领域:
生物化学
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分析化学
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分子生物学
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DOI
PubMed
高影响因子
Russian Journal of Physical Chemistry A
·
2020
·Vol. 94
·期 5
·1067-1072
·
IF 0.719
Liang An,Meng Yuan,Wang Tao,Xiong Chao,Yan Zhaoxiong,Xu Zhihua
Abstract A new magnetic Ag 3 PO 4 –TiO 2 –Fe 2 O 3 photocatalyst (Ag–Ti–Fe) has been synthesized by a solid-state blending method. The sample was analyzed by XRD, FT-IR, and VSM, and its photocatalytic performance in treatment of simulated dye-contaminated wastewater was evaluated in photocatalytic tests. SEM and FT-IR results confirmed that Ag–Ti–Fe catalyst was composed of Ag 3 PO 4 , anatase and maghemite. Results of photocatalytic tests revealed that Ag–Ti–Fe exhibited the highest photocatalytic activity on water treatment compared with contrastive samples whether the light source was UV or visible light, and the ultimate degradation efficiency of orange II reached 86.71 and 78.13%, respectively. Ag–Ti–Fe could decompose chemically stable groups, rather than simply destroy chromogenic azo double bonds, and it could further effectively remove methyl orange, rhodamine B, methylene blue from water, respectively. VSM results showed that the saturation magnetization of Ag–Ti–Fe was 4.80 emu/g, and it could be separated simply and quickly with the magnetic field.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2014
·Vol. 131
·期 19
·
IF 1.64
Jiangdong Dai,Zhiping Zhou,Yongli Zou,Xiao Wei,Xiaohui Dai,Chunxiang Li,Yongsheng Yan
A surface imprinted core-shell nanorod with water-compatible property was first prepared, using a two-step “living” polymerization technique, with magnetic attapulgite (MATP) as core, and it was applicable in the enhanced selective removal of sulfamethazine residue from pure water environments. MATP was synthesized by an impregnation and pyrolysis method, and polymerable group was subsequently attached onto the surface. The imprinted polymer nanoshell (13 nm) with the “living” fragments was formed via a reverse atom transfer radical precipitation polymerization, avoiding the tedious graft of initiator and providing the easy-accessible imprinted sites. Ultrathin hydrophilic polymer brushes (2.0 nm) were surface-grafted to improve their water-compatibility. The nanoadsorbent exhibited good thermal stability, magnetism, and hydrophilicity through characterization. The nanoadsorbent showed large adsorption capacity toward sulfamethazine from water, which increased with the increase of contact temperature. Langmuir isotherm fitted the equilibrium data better, and the kinetic data (within 45 min) were well-analyzed by the pseudo-second-order kinetic model. Also, the specific adsorption property of the nanoadsorbent was greatly improved through the surface-grafting, which exhibited excellent selectivity to sulfamethazine compared with other reference antibiotics. Efficient magnetic separation and good reuse of the nanoadsorbent provided the potential possibility for selective recognition and fast removal of antibiotic pollutions from water environments. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131 , 40854.
研究领域:
材料科学
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环境科学
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分子生物学
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Peng Cheng,Chuang Liu,Sicong Du,Chongxuan Xu,Xu Han,Ting Zhu,Wenyan Liang
A novel magnetic separation method has been developed to efficiently separate and recover powder particles used in water or wastewater treatment processes. The magnetic composites were prepared through the grafting of 2,4-bis(dimethylamino)-6-chloro-(1,3,5)-triazine (BDAT), chitosan (CTS), and SiO 2 onto the Fe 3 O 4 surface, obtaining Fe 3 O 4 @BDAT, Fe 3 O 4 @CTS, Fe 3 O 4 @CTS-BDAT, Fe 3 O 4 @SiO 2 @BDAT, and Fe 3 O 4 @SiO 2 @CTS-BDAT. g-C 3 N 4 , Ni/Fe-PAC, and La/Al-BTC were chosen as the target particles. Results showed that Fe 3 O 4 @CTS-BDAT exhibited the best separation performance, achieving efficiencies of 98.3 %, 97.2 %, and 99.1 % for the three target particles, respectively. These high efficiencies were maintained over a broad pH range (3.0–11.0) and initial target particle concentrations (50–5000 mg/L). The target particles could be detached from the magnetic aggregates using an electromagnetic field, with efficiencies exceeding 75.3 %. Characterization using SEM, XPS, and FTIR revealed that Fe 3 O 4 @CTS-BDAT possessed a microsphere cluster structure, with a saturation magnetization of 64.02 emu/g, a point of zero charge (pH PZC ) of 7.7, and a d 50 value of 3.56 μm. Microscopic image analysis displayed that fluid kinetic energy and magnetic dipole force facilitated the formation of magnetic aggregates during the stirring stage. The size of these aggregates increased from 2–10 to 15–30 μm during stirring and further expanded to 18–37 μm during the separation stage. Particle image velocimetry (PIV) analysis indicated that the magnetic aggregates migrated towards the magnet via eddy currents, with maximum velocity from 0.532 to 1.412 m/s. DLVO and MDLVO models revealed that the magnetic interaction energy of Fe 3 O 4 @CTS-BDAT was sufficient to surpass electrostatic repulsion, thereby enabling efficient capture and separation of target particles.
研究领域:
环境科学
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材料科学
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化学工程
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
He Dongxia,Liu Qi,Wang Lei,Han Qinqin,Zhang Jinyang,Li Chao,Song Yuzhu
A novel strategy integrating Brevinin-1BW (BW)-functionalized magnetic bead-based separation with a colorimetric immunoassay was developed to concentrate and enrich Staphylococcus aureus ( S. aureus ) from sample matrices using magnetic beads functionalized with antimicrobial peptide BW (MBs-BW) as carriers. To ensure the selectivity of the method, horseradish peroxidase (HRP)-labeled porcine IgG (HRP-porcine IgG) was used as a molecular recognition reagent and signal amplification probe. MBs-BW/ S. aureus /HRP-porcine IgG probe sandwich complex was successfully obtained. HRP chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) was used to realize the chromogenic reaction. Finally, S. aureus was quantitatively analyzed based on the color signal generated. Under the optimal conditions, the assay showed a response in the concentration range of 1.0 × 10 2 –1.0 × 10 7 CFU/mL of S. aureus , and the detection limit was as low as 60 CFU/mL. This method does not require complicated sample pretreatment, and the operation process is fast and simple, which can directly complete the whole process of bacterial enrichment and detection. The recovery of this strategy in different types of spiked samples reached 86.8–97.8%, and the analytical results of this strategy were highly consistent with those of the conventional plate counting method. This strategy provides a new way for the rapid detection of S. aureus in complex samples. Graphical
研究领域:
微生物学
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生物化学
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分析化学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2020
·
IF 10.652
Xiaojie Li,Bin Liu,Yongmin Lao,Peng Wan,Xuemei Mao,Feng Chen
Magnetic separation represents a cost-effective and energy-saving strategy for harvesting targets of interest, e.g., small molecules, biomacromolecules, and cells, in complex mixture. Conventional approaches necessitate multi-step time-consuming processes, including the pre-synthesis of magnetic nanoparticles and subsequent surface modification with targeting ligands. Here, a biocompatible and rapid separation strategy was developed for six microalgal species by in situ formation of iron nanoparticles on the microalgal surface. This method streamlines the separation process of various species of microalgae (e.g., Chlorella zofingiensis ) from culture medium with a high harvesting efficiency (greater than 95%) within 1 min spanning a wide pH range of 4–11. Moreover, the recovered microalgae showed intact viability and exhibited no deterioration effect on the downstream extraction process of high-value bioproducts (e.g., chlorophyll, carotenoids and lipids). This method not only provides an alternative to harvest microalgae in a facile manner, but also demonstrates a new pathway to separate other microorganisms and cells, providing more opportunities in economic bioengineering and bioresource production.
研究领域:
生物工程
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2018
·
IF 5.091
Baojun Ding,Jie Wang,Shengyang Tao,Yunzhe Ding,Lijing Zhang,Ning Gao,Guangtao Li,Haonan Shi,Weijun Li,Shuo Ge
Water pollution control has become significant challenges in recent years because of their extensive species diversity. It is critical to developing general-purpose materials for environmental rehabilitation. In this paper, a novel module-assembly method is developed to prepare multi-functional materials for treating pollutants in water. Building blocks are porous nanoparticles with a different function. Microspheres (MS) with a diameter of 90 μm are prepared and have a coefficient of variation of 6.8%. The modular fashion of self-assembly process in a microfluidic chip is the crucial factor in fabricating the multifunction material. The assembled microspheres with different building modules still have a specific surface area larger than 400 m 2 g −1 , and exhibit excellent performance in adsorbing various pollutants in water, such as heavy metal ions and organic dyes. The adsorption capacities of them to Hg 2+ and orange II reach 150 mg g −1 and 333 mg g −1 , respectively. The integrated fluorescence probes in microspheres can detect low concentration (9.8 ppb) of Hg 2+ . Microspheres integrated with Fe 3 O 4 nanoparticles have a magnetic susceptibility of 6.01 emu g −1 and can be easily removed from wastewater by applying an external magnetic. Due to the stability of inorganic building blocks, each function in the assembled system is well performed, and multi-functional “All-in-One” materials can be easily fabricated.
研究领域:
环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 105
·103585-103593
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IF 3.289
Junfeng Zhao,Jiachen Zha,Hongbin Lu,Cheng Yang,Kou Yan,Xiangkang Meng
Cauliflower-like nickel alkoxide, Ni/NiO and NiO architectures were synthesized via a reflux route using NaBH4–EG as alkaline precipitant. The as-prepared powders were characterized by XRD, FESEM, TEM, HRTEM, and N2 adsorption/desorption isotherms. The results show that the nickel alkoxide is made up of smooth spherical building blocks with diameters of about 500 nm that aggregate interactively to form whole 3D structures. The Ni/NiO and NiO architectures can be obtained by the direct thermal transformation from nickel alkoxide precursor at 300 °C and 600 °C, respectively, which are assembled by nanoparticles and composed of mesopores and macropore. The as-prepared three samples exhibit excellent performance for the removal of Congo red (CR) and Cr(VI) pollutant from aqueous solutions due to their hierarchical structures and high specific surface areas. The corresponding adsorption process fits well with the pseudo-second-order kinetics model and the Langmuir model. Compared with nickel alkoxide and NiO, Ni/NiO architectures show the highest adsorption performance, with the maximum capacity of 642.37 and 92.52 mg g−1 for CR and Cr(VI), respectively. Further, the easily magnetic separation and reused clearly suggests that the Ni/NiO architectures can be explored as an excellent adsorbent for the removal of organic dye and heavy metal ions from aqueous solutions.
研究领域:
材料科学
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环境科学
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产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2015
·
IF 3.368
X. Ge,C.D. Gu,X.L. Wang,J.P. Tu
Adsorption is often time consuming due to slow diffusion kinetic. Sizing he adsorbent down might help to accelerate adsorption. For CoFe spinel oxide, a magnetically separable adsorbent, the preparation of nanosheets faces many challenges including phase separation, grain growth and difficulty in preparing two-dimensional materials. In this work, we prepared porous CoFe oxide nanosheet with chemical formula of Co 2.698 Fe 0.302 O 4 through topochemical transformation of a CoFe precursor, which has a layered double hydroxide (LDH) analogue structure and a large interlayer spacing. The LDH precursor was synthesized from a cheap deep eutectic solvent (DES) system. The calcined Co 2.698 Fe 0.302 O 4 has small grain size (10–20 nm), nanosheet morphology, and porous structure, which contribute to a large specific surface area of 79.5 m 2 g −1 . The Co 2.698 Fe 0.302 O 4 nanosheets show fast removal ability and good adsorption capacity for both organic waste (305 mg g −1 in 5 min for Congo red) and toxic heavy metal ion (5.27 mg g −1 in 30 min for Cr (VI)). Furthermore, the Co 2.698 Fe 0.302 O 4 can be separated magnetically. Considering the precursor can be prepared through a fast, simple, surfactant-free and high-yield synthetic strategy, this work should have practical significance in fabricating adsorbents.
研究领域:
材料科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MINERAL PROCESSING
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2017
·
IF 1.561
Yichang Yu,Zhangjun Hu,Yue Wang,Hongwen Gao
A magnetic nano-scaled composite was synthesized by self-assembly of cationic surfactant octadecyl dimethyl hydroxyethyl ammonium nitrate (SN) into natural mineral diatomite via a ‘dissolution and reassembly’ method. Fourier transform infrared (FT-IR), elemental analysis, scanning electron microscopy (SEM), transmission electron microscope (TEM), surface area and magnetic analysis were performed to characterize the achieved composites. The results indicated that SN and Fe 3 O 4 nanopartilces were successfully inserted into the diatomite matrix. Under the same condition, the mixed phenols adsorption experiments showed that the maximum adsorption capacity of phenols by the composite was in order of 2,4,6-trichlorophenol > 2,6-dichlorophenol > 2,4-dichlorophenol > p-nitrophenol > m-methylphenol > phenol, the same as the competitive adsorption ability. The adsorption process obeyed the Langmuir isothermal model. 2,6-Dichlorophenol was used as a representative contaminant to further investigate the mechanism of adsorption. Electrostatic interaction and hydrophobic stacking effect might play important roles in the adsorption towards organic phenols. Owing to the embedded magnetic Fe 3 O 4 , the composite was able to be separated easily from liquid media under an external magnetic field. The further calcination of the used composites showed a possibility to deliver new magnetic mesoporous absorbents for further use.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2021
·Vol. 36
·期 2
·e6513
·
IF 4.105
Haifeng Zhang,Cheng Cai,Tao Hu,Zhijie Zhang,Lina Dai,Huafeng Fei,Hongli Bai,Chonggang Wu,Xinghou Gong,Xuan Zheng
Understanding the interaction between metal and the support is crucial in searching of efficient and recyclable noble metal heterogeneous catalysts. Herein, we report a core-shell structured support for Pt catalyst with a magnetic Fe 3 O 4 core and a porous silica shell bearing amino groups. The prepared catalyst Fe 3 O 4 @mSiO 2 -APTES-Pt showed a high loading of Pt (7.93 wt%) as well as a high fraction of active Pt 0 species, which both contributed to the high activity toward a model hydrosilylation reaction between 1-octene and 1, 1, 1, 3, 5, 5, 5-heptamethyltrisiloxane (HMTS). X-ray photoelectron spectra (XPS) analysis revealed that the amino ligands played a dominant role in Pt loading and Fe 2+ probably facilitated the formation of low valence Pt 0 . Under the combined effect of magnetic nature of the support and proper support-metal interactions, the catalyst exhibited excellent recyclability with a recovery of 99.7% and a yield of over 90% even after eight runs of recycling. This catalyst may provide a new option in designing and preparing heterogeneous catalysts that are highly recoverable, reusable, and recyclable.
研究领域:
催化化学
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材料科学
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产品关联
DOI
高影响因子
SYNTHETIC METALS
·
2022
·
IF 4
Afzal Ali,Tahir Raza,Adeel Ahmed,Muhammad Safdar Ali,Changbao Liu,Dong Li,Chunhu Li
The intentional discharge of polycyclic aromatic hydrocarbons (PAHs) into the aquatic system from different industrial units has posed a serious threat to human health. To address the concerns associated with PAHs misuse, an efficient approach for developing affordable photocatalyst materials for PAHs degradation is much needed. In this study, we have explored co-precipitation and hydrothermal methods to synthesize Fe 3 O 4 /GO/g-C 3 N 4 ternary composites and utilized them as photocatalyst materials for the degradation of naphthalene. Fe 3 O 4 /GO/g-C 3 N 4 nanocomposite demonstrated superior degradation performance, with 87.56% naphthalene degradation achieved in 140 min under visible light irradiation, far outperforming Fe 3 O 4 /GO (77.42%) and Fe 3 O 4 (69.42%). The enhanced degradation of naphthalene by the Fe 3 O 4 /GO/g-C 3 N 4 composite was credited to the effective charge carrier separation and increased visible-light absorption induced by GO and g-C 3 N 4 . The impact of operating parameters on the catalytic efficiency of the Fe 3 O 4 /GO/g-C 3 N 4 catalyst was also examined. The findings of this study revealed that lower naphthalene concentration (10 mg/L), higher catalyst dosage (40 mg), and moderate pH conditions (pH 5 to pH 9) were favorable for photocatalytic activity. The degradation of naphthalene followed pseudo-first-order kinetics with a rate constant value of 0.0137 min −1 . The generation of radicals ( • OH and O 2 •− ) liable for the degradation of naphthalene was corroborated through electron paramagnetic resonance analysis and quenching experiments. Furthermore, the Fe 3 O 4 /GO/g-C 3 N 4 photocatalyst exhibited promising reusability in five consecutive cyclic runs, validating its excellent stability.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）