Gels
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2023
·Vol. 9
·期 5
·373
·
IF 4.6
Qiulin Wang,Xiaoyu Wang,Yakai Feng
Chitosan hydrogels have a wide range of applications in tissue engineering scaffolds, mainly due to the advantages of their chemical and physical properties. This review focuses on the application of chitosan hydrogels in tissue engineering scaffolds for vascular regeneration. We have mainly introduced these following aspects: advantages and progress of chitosan hydrogels in vascular regeneration hydrogels and the modification of chitosan hydrogels to improve the application in vascular regeneration. Finally, this paper discusses the prospects of chitosan hydrogels for vascular regeneration.Keywords:chitosan;hydrogel;tissue engineering;vascular regeneration;angiogenesis
研究领域:
生物材料
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组织工程
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再生医学
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产品关联
DOI
PubMed
高影响因子
Annals of Translational Medicine
·
2021
·
IF 3.932
Jing Lu,Wenjie Tian,Linxian Cui,Bing Cai,Tingting Zhang,Nan Huang,Lei Lu,Tao Zhu
Background Lidocaine (LDC) is a local anesthetic widely used to relieve intubation-related airway responses. However, low drug concentration and short effective duration of LDC is inadequate to provide a satisfactory anesthetic effect on the surface of the airway. The present study sought to develop a LDC-delivery endotracheal tube (ETT) to achieve high local drug concentration and sustained drug release with the aim of attenuating an intubation-related airway response. Methods ETTs and polyvinyl chloride (PVC) discs were coated with different molecular weight (MW) poly lactic-co-glycolic acid (PLGA: 50/50; MW: 3,000, 6,000, and 10,000) loaded with LDC by airbrush spray. The morphology of LDC-eluting coatings was analyzed using scanning electron microscopy. In vitro drug release was determined by ultraviolet spectrophotometer. An in vivo study was performed to investigate the differences in plasma LDC concentration, intubation tolerance, and tracheal tissue injury in rabbits undergoing intubation of blank, LDC-spray, or LDC-coated ETTs. Results Approximate 5 mg/cm 2 coatings (containing 2.5 mg/cm 2 LDC) were deposited onto the PVC discs and ETTs. While even distribution and smooth surfaces were generated in PLGA3000 + LDC and PLGA6000 + LDC coatings, PLGA10000 + LDC formed uneven and gullied coatings. Burst release within the first 4 h and sustained release for at least 5 days was achieved in vitro in PLGA + LDC coatings and the in vivo study demonstrated higher plasma LDC concentration and longer drug release duration in LDC-coated ETTs compared with LDC-spray. LDC-coated ETTs significantly improved intubation tolerance in rabbits, as measured by less general anesthetic consumption and longer tube tolerance duration in contrast to blank ETTs with or without LDC spray. Histology assessment showed less mucosal edema area in the PLGA3000 + LDC and PLGA6000 + LDC groups compared to the control, LDC-spray, and PLGA10000 + LDC groups. Among the different MW PLGAs, PLGA6000 presented optimal morphological characteristics, drug release, and anesthetic effect. Conclusions ETTs coated with PLGA + LDC effectively attenuate an intubation-related airway response via increasing local drug concentration and extending drug action duration, which demonstrates a potential therapeutic benefit for patients undergoing intubation.
研究领域:
麻醉学
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药剂学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Journal of Translational Medicine
·
2023
·Vol. 21
·期 1
·1-25
·
IF 7.4
Ren Jun-Song,Bai Wen,Ding Jing-Juan,Ge Hui-Min,Wang Su-Yu,Chen Xi,Jiang Qin
Background Pathological neovascularization plays a pivotal role in the onset and progression of tumors and neovascular eye diseases. Despite notable advancements in the development of anti-angiogenic medications that target vascular endothelial growth factor (VEGF) and its receptors (VEGFRs), the occurrence of adverse reactions and drug resistance has somewhat impeded the widespread application of these drugs. Therefore, additional investigations are warranted to explore alternative therapeutic targets. In recent years, owing to the swift advancement of high-throughput sequencing technology, pan-cancer analysis and single-cell sequencing analysis have emerged as pivotal methodologies and focal areas within the domain of omics research, which is of great significance for us to find potential targets related to the regulation of pathological neovascularization.Methods Pan-cancer analysis and scRNA-seq data analysis were employed to forecast the association between Actin filament-associated protein 1 like 1 (AFAP1L1) and the development of tumors and endothelial cells. Tumor xenograft model and ocular pathological neovascularization model were constructed as well as Isolectin B4 (IsoB4) staining and immunofluorescence staining were used to assess the effects of AFAP1L1 on the progression of neoplasms and neovascular eye diseases in vivo. Transwell assay, wound scratch assay, tube forming assay, three-dimensional germination assay, and rhodamine-phalloidin staining were used to evaluate the impact of AFAP1L1 on human umbilical vein endothelial cells (HUVECs) function in vitro; Dual luciferase reporting, qRT-PCR and western blot were used to investigate the upstream and downstream mechanisms of pathological neovascularization mediated by AFAP1L1.Results Our investigation revealed that AFAP1L1 plays a crucial role in promoting the development of various tumors and demonstrates a strong correlation with endothelial cells. Targeted suppression of AFAP1L1 specifically in endothelial cells in vivo proves effective in inhibiting tumor formation and ocular pathological neovascularization. Mechanistically, AFAP1L1 functions as a hypoxia-related regulatory protein that can be activated by HIF-1α. In vitro experiments demonstrated that reducing AFAP1L1 levels can reverse hypoxia-induced excessive angiogenic capacity in HUVECs. The principal mechanism of angiogenesis inhibition entails the regulation of tip cell behavior through the YAP-DLL4-NOTCH axis.Conclusion In conclusion, AFAP1L1, a newly identified hypoxia-related regulatory protein, can be activated by HIF-1α. Inhibiting AFAP1L1 results in the inhibition of angiogenesis by suppressing the germination of endothelial tip cells through the YAP-DLL4-NOTCH axis. This presents a promising therapeutic target to halt the progression of tumors and neovascular eye disease.
研究领域:
肿瘤学
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分子生物学
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细胞生物学
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眼科学
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DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-14
·
IF 15.7
Liu Zhirong,Tang Chuyu,Han Nannan,Jiang Zhuoheng,Liang Xi,Wang Shaobo,Hu Quanhong,Xiong Cheng,Yao Shuncheng,Wang Zhuo,Wang Zhong Lin,Zou Duohong,Li Linlin
Patients suffering from coronary artery disease (CAD) or peripheral arterial disease (PAD) can benefit from bypass graft surgery. For this surgery, arterial vascular grafts have become promising alternatives when autologous grafts are inaccessible but suffer from numerous postimplantation challenges, particularly delayed endothelialization, intimal hyperplasia, high risk of thrombogenicity and restenosis, and difficulty in timely detection of these subtle pathological changes. We present an electronic vascular conduit that integrates flexible electronics into bionic vascular grafts for in situ, real-time and long-term monitoring for hemadostenosis and thrombosis concurrent with postoperative vascular repair. Following bypass surgery, the integrated bioelectronic sensor based on the triboelectric effect enables monitoring of the blood flow in the vascular graft and identification of lesions in real time for up to three months. In male nonhuman primate cynomolgus monkeys, the electronic vascular conduit, with an integrated wireless signal transmission module, enables wireless and real-time hemodynamic monitoring and timely identification of thrombi. This electronic vascular conduit demonstrates potential as a treatment-monitoring platform, providing a sensitive and intuitive monitoring technique during the critical period after bypass surgery in patients with CAD and PAD.
研究领域:
生物医学工程
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心血管病学
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转化医学
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植入式医疗器械
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分子影像学
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生理监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR MEDICINE-JMM
·
2025
·
IF 4.2
Wen Xin,Peng Yuefeng,Yang Wenqing,Zhu Yuzhong,Yu Fan,Geng Li,Wang Xianfeng,Wang Xiaoyan,Zhang Xiaodong,Tang Yi,Feng Lei,Zhou Tingting,Jia Hongliang,Yang Liu
Transient receptor potential canonical 1 (TRPC1) channel, a Ca 2+ -permeable ion channel widely expressed in vasculature, has been reported to be involved in various cardiovascular disorders. However, the pathophysiological function of vascular smooth muscle cell (VSMC)-derived TRPC1 in hypertension and hypertensive cardiovascular remodeling remains to be defined. In this study, we found increased TRPC1 expression in both angiotensin II (AngII)-treated VSMCs and aortas from AngII-infused mice. VSMC-specific TRPC1 deficiency strikingly attenuated AngII-induced vasoconstriction, hypertension, vascular remodeling, and cardiac hypertrophy. Mechanistically, AngII activated enhancer of zeste homolog 2 (EZH2) to stimulate TRPC1 expression, induced calcium influx and phosphorylation of mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK-ERK), which in turn triggered VSMC proliferation and migration and exacerbated hypertension and cardiovascular remodeling. Treatment with EZH2 inhibitor reduced VSMC proliferation and migration and alleviated vasoconstriction and hypertension in AngII-infused mice. Together, we revealed the pathogenic role of the EZH2-TRPC1-MEK/ERK pathway in AngII-induced hypertension and cardiovascular damage. TRPC1 or EZH2 inhibition may represent a desirable therapeutic target for the treatment of hypertension. Key messages
AngII activates AT1R-EZH2-TRPC1 pathway in VSMCs and aortas of hypertensive mice.
TRPC1 promotes VSMC proliferation and migration via MEK/ERK signaling.
Inhibition of TRPC1 or EZH2 alleviates hypertension and cardiovascular remodeling.
研究领域:
心血管病学
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分子生物学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CELLULOSE
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2025
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IF 4.8
Xu Qingbo,Zhang Jinlong,Zhang Supeng,Wu Zheng,Tao Xuchen
Adaptive moisture and thermal management cotton fabrics can maintain human physiological comfort without requiring space heating or cooling, aligning with the goals of sustainable development, carbon neutrality, and carbon peak. However, the moisture and thermal management performance and wearing comfort of cotton fabrics require further study. In this study, mist polymerization technology and the “grafting through” polymerization method were used to graft poly[2-(2-methoxyethoxy) ethoxyethyl methacrylate] (PMEO2MA) onto the inner surface of cotton fabric and poly[ N,N -dimethyl(methacryloylethyl)ammonium propane sulfonate] (PDMAPS) onto the outer surface. The reversible wetting properties of both surfaces impart the fabric with excellent moisture and thermal management capabilities. The treated fabric reduces skin temperature by approximately 5.0 °C within 8 min when worn at 31.0 °C and increases skin temperature by around 3.0 °C–4.0 °C within 8 min when worn at 9.5 °C. This fabric, capable of rapidly managing moisture and thermal conditions in a short duration, exhibits considerable potential for application in harsh and demanding environments.
研究领域:
材料科学
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纺织工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2024
·Vol. 90
·期 1
·e17647
·
IF 3.2
Jingyu Gao,Fan Wu,Mingzhu Yan,Xuejiang Wang,Yan Chi,Yu Zhang,Yu Peng,Mo Li,Yuanying Ni,Xin Wen
Brown seaweed oligosaccharides (BSO) have demonstrated potential as nutraceuticals with cholesterol-lowering, anti-obesity, and anti-constipation properties. In this study, we initially examined how BSO impact body weight, blood lipid levels, and adipose tissue in a rat model of obesity induced by a high-fat diet. Our findings revealed that BSO administration significantly attenuated body weight gain, ameliorated dyslipidemia, and reduced visceral adiposity. Additionally, we evaluated the prophylactic efficacy of BSO in a loperamide hydrochloride-induced constipation mouse model by assessing defecation parameters and gastrointestinal motility. Our results indicated that BSO intervention significantly increased fecal pallet output and fecal water content, as well as improved intestinal transit. In conclusion, this study lays the foundation for a deeper understanding of how BSO may influence obesity and constipation, offering novel insights into its potential safe and efficacious clinical applications.
研究领域:
营养学
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代谢生物学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Progress in Natural Science-Materials International
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2015
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IF 1.873
Chao Yang,Chengyi Song,Wen Shang,Peng Tao,Tao Deng
In this work we report the facile fabrication and performance evaluation of flexible heat pipes that have integrated bioinspired wick structures and flexible polyurethane polymer connector design between the copper condenser and evaporator. Inside the heat pipe, a bioinspired superhydrophilic strong-base-oxidized copper mesh with multi-scale micro/nano-structures was used as the wicking material and deionized water was selected as working fluid. Thermal resistances of the fabricated flexible heat pipes charged with different filling ratios were measured under thermal power inputs ranging from 2 W to 12 W while the device was bent at different angles. The fabricated heat pipes with a 30% filling ratio demonstrated a low thermal resistance less than 0.01 K/W. Compared with the vertically oriented straight heat pipes, bending from 30° up to 120° has negligible influence on the heat-transfer performance. Furthermore, repeated heating tests indicated that the fabricated flexible heat pipes have consistent and reliable heat-transfer performance, thus would have important applications for advanced thermal management in three dimensional and flexible electronic devices.
研究领域:
材料科学
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热能工程
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仿生学
✓ 已验证
产品关联
DOI
高影响因子
NANO
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2014
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IF 1.26
JUN LI,BIN KANG,SHUQUAN CHANG,YAODONG DAI
We directly visualized the uptake and intracellular vesicle transport of shortened single walled carbon nanotubes in living cells through a dual-labeling system. With a stable labeling of fluorescein isothiocyanate and a pH-sensitive stacking of doxorubicin on carbon nanotubes, the location of internalized nanotubes inside the cell and the microenvironment especially the pH change of the nanotube-embedded vesicles could be monitored at the same time. Results showed that after internalization through endocytic pathway, carbon nanotubes tended to transport from the early endosomes to later acidic lysosomes and these vesicles moved along the microtubule track toward a perinuclear region where is a microtubule-organizing center. These results might provide a novel understanding for the intracellular interaction of carbon nanotubes and living cells.
研究领域:
细胞生物学
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纳米医学
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生物成像
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Zui Tao,Hewei Si,Xidong Zhang,Jianjun Liao,Shiwei Lin
A highly sensitive and selective sensor is urgently needed in many practical H 2 O 2 detection, such as water quality monitoring, quality control of food products, and so on. However, the traditional three electrode system limits its further development in recent years due to its difficulty in miniaturization and integration. To effectively improve its sensitivity and selectivity, highly-ordered TiO 2 nanotube arrays (TNTAs) are modified with Pt nanoparticles and Prussian blue (PB) nanotubes and creatively combines with thin-film transistor (TFT) based on ZnO to prepare an electrochemical sensor . The sensor uses PBNCs as recognition units for the specific detection of H 2 O 2, and the generated electrical signal is amplified by TFT. Thus, a highly sensitive and selective H 2 O 2 sensor is achieved. The detection limit of the sensor is as low as 5.19 nM, which is three orders of magnitude lower than the conventional electrochemical detection methods using the same electrode. The sensor also possesses wider linear detection range of 0.1–50 μM and 50 μM–5 mM comparable to that of other reported H 2 O 2 sensors. Besides, the sensor has good selectivity for H 2 O 2 in the presence of common interferences such as ascorbic acid , uric acid , glucose, and dopamine. Furthermore, the device presents good reproducibility and exhibits good long-term stability over 27 days of continuous measurement. This study offers important insights for the rational design of sensors with high sensitivity and selectivity.
研究领域:
生物医学工程
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材料科学
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分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF POWER SOURCES
·
2020
·
IF 8.247
Xiaotian Wu,Shaodi Zheng,Yanhao Huang,Zewang Xu,Zhengying Liu,Wei Yang,Mingbo Yang
The carboxylic acid functionalized multi-walled carbon nanotubes/polyaniline nanotubes (PANI-NTs)/carboxylic acid functionalized single-walled carbon nanotubes electrodes for the flexible supercapacitors are fabricated by the layer-by-layer vacuum filtration. This strategy is aimed to assist powdery PANI-NTs to form a binder-free electrode, and meanwhile avoid sufficient contact between PANI-NTs and carbon nanotubes, ensuring abundant channels with a low tortuosity for enhanced electrolyte permeation and ion diffusion as well as large ion available surface area. Consequently, the assembled all-solid-state device achieves a high areal capacitance (258 mF cm −2 at current density of 0.5 mA cm −2 ), a superior energy density (3.82 m Wh cm −3 at a power density 33 mW cm −3 ) and favorable cycle stability (75.1% capacitance retention after 5000 cycles). Furthermore, the supercapacitor possesses outstanding flexibility and can be 180° bended up to 1000 times without loss of capacitance. Our works reveal that electrode structure design and fabrication procedures are also significant factors that greatly dominates electrochemical performance of supercapacitors.
研究领域:
材料科学
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能源存储技术
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
·
2010
·
IF 7.365
De-Hong Yu,Qin Lu,Jing Xie,Chao Fang,Hong-Zhuan Chen
Antiangiogenic cancer therapy can be achieved through the targeted delivery of antiangiogenic agents to the endothelial cells of tumor neovasculature. In the present study, we developed a drug delivery system (DDS), nanoparticles conjugated with K237-(HTMYYHHYQHHL) peptides for tumor neovasculature targeting drug delivery. Paclitaxel, a chemotherapeutic agent with potent antiangiogenic activity , was used as a prototype drug. We synthesized the aldehyde poly(ethylene glycol)–poly(lactide) (aldehyde-PEG–PLA) block copolymer by ring opening polymerization . The nanoparticles loading paclitaxel (PTX-NP) were fabricated using the O/W emulsion and evaporation technique. K237 ligand, a peptide that can bind to the KDR receptors predominantly expressed on the surface of tumor neovasculature endothelial cells with high affinity and specificity and inhibit the VEGF-KDR angiogenic signal pathway, was conjugated to the aldehyde group of PEG chain using the N-terminal PEGylation technique. The K237 conjugated paclitaxel-loaded nanoparticles (K237-PTX-NP) had a hydrodynamic diameter of 150 nm. The K237 density on nanoparticle surface was 474 and the mean distance between two neighboring PEG chains linked to K237 peptide was 12 nm. The K237 conjugated nanoparticles could be significantly internalized by human umbilical vein endothelial cells (HUVEC) through the K237-KDR interaction, and this facilitated uptake led to the expected enhanced antiangiogenic activity shown by HUVEC proliferation , migration and tube formation compared to cells treated with the commercial formulation Taxol ® and PTX-NP. The long-circulating property and the K237 ligand of K237-PTX-NP warranted rapid, long-term, and accurate in vivo tumor neovasculature targeting, and thereafter the significant apoptosis of tumor neovasculature endothelial cells and necrosis of tumor tissues of MDA-MB-231 breast tumors implanted in female BLAB/c nude mice . This nanoparticulate DDS offers a new strategy for paclitaxel chemotherapy application and it could also be used to carry other chemotherapeutic drugs, genes, and proteins with antiangiogenic activity for antiangiogenic cancer therapy.
研究领域:
药学
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肿瘤学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）