SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Donglian Deng,Jingyuan Xu,Tingting Li,Dongdong Tan,Yibing Ji,Ruijun Li
This work designed a fluorometric/colorimetric dual-mode sensor for detecting 2,6-dipicolinic acid (DPA) based on the blue emission property and peroxidase-like activity of Fe-MIL-88NH 2 . The fluorescence of Fe-MIL-88NH 2 was obviously turned off by Cu 2+ , but DPA was able to bring it back because it has a strong chelate bond with Cu 2+ . Fe-MIL-88NH 2 also displayed high peroxidase-like activity, which accelerated the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to the blue oxidation product (oxTMB) when H 2 O 2 was present. When DPA was added, it efficiently inhibited the peroxidase-like activity of Fe-MIL-88NH 2 , causing less oxTMB and less absorbance at 652 nm. The fluorescence recovery of Fe-MIL-88NH 2 and the change in absorbance at 652 nm were used as analytical signals for dual-mode detection of DPA. The linear responses in the range of 10–60 μM and 60–160 μM were achieved for the fluorometric mode, and the limit of detection (LOD) was 1.46 μM. The respective values of linear range and LOD for the colorimetric mode were 5–25 μM and 3.00 μM, respectively. In summary, the dual-mode testing strategy successfully detected DPA in aqueous environmental samples, suggesting great potential in disease prevention and environmental analysis.
研究领域:
分析化学
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环境科学
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生物传感
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Xiaolan Yang,Jitao Li,Qiulan Li,Dezhi Yang,Yaling Yang
The amorphous Cu-containing phosphomolybdate (Cu-PTs) composite with high peroxidase (POD)-like activity at neutral conditions was explored as biosensors for raloxifene (RAF) detection. The strong attraction between negatively charged Cu-PTs and positively charged substrates 3,3′,5,5′-tetramethylbenzidine (TMB), as well as the acceleration of the conversion of active Cu + /Cu 2+ by the Cu/W bimetallic redox couples were demonstrated to play significant roles in POD-like activity in physiological environment. When RAF is presence, it can bind to the surface of Cu-PTs and changes the chemical signal on the material surface, leading to the decreased POD-like activity. Based on this, a colorimetric method was established for the sensitive assay of RAF with a lower limit of detection (LOD) of 0.025 mg/L and good recovery from 90.13% to 108.9%. This work paves a new way to design a POD-like colorimetric protocol for tracing RAF in pharmaceutical products and environmental samples.
研究领域:
分析化学
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生物传感
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环境科学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 37
·5484-5493
·
IF 1.9
Wenping Yang,Yuangen Wu,Han Tao,Jing Zhao,Huayun Chen,Shuyi Qiu
This paper reports a new colorimetric aptasensor for the detection of acetamiprid pesticides with high sensitivity and selectivity based on the enhanced peroxidase-like activity of gold nanoparticles (AuNPs). The aptasensor employs an S-18 aptamer as the target specific recognition molecule and the enhanced peroxidase-like activity of AuNPs as the signal transmission element. In the absence of acetamiprid, S-18 aptamer sequences can adsorb on the surface of AuNPs and enhance their negative charge density because of the attached negatively charged DNA backbone, which will inhibit the peroxidase catalytic activity of the AuNPs, thus inhibiting the oxidation of the substance 2,2′-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) (ABTS). In the presence of acetamiprid, aptamer sequences can specifically bind with pesticide molecules to form an acetamiprid–aptamer complex which can interact with the AuNPs’ surface through the adsorption energy of the nucleobases. Thus, the surface active sites of the AuNPs are activated and their catalytic activity is enhanced greatly, which enables the AuNPs to catalyze the oxidation of ABTS to generate green oxidation products with a characteristic absorption peak at 735 nm. The color variation is relevant to the concentration of acetamiprid, which can be determined using a UV-Vis spectrometer or even by the naked eye. The present colorimetric aptasensor has a dynamic range of 10–160 μg L−1 with a limit of detection as low as 1.02 μg L−1 and high selectivity against other competitive pesticides. Moreover, the proposed aptasensor can accurately and efficiently screen out acetamiprid in representative actual samples that contain trace levels of pesticides, suggesting that it has more advantages than traditional methods for the detection of trace pesticide residues. Such an ultrasensitive aptasensor will play a prominent part in agricultural product and environmental detection.
研究领域:
农业科学
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环境科学
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纳米技术
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分析化学
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DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Zhiyang Qin,Tianyue Peng,Xinguang Qin,Gang Liu,Haizhi Zhang
Accurate detection of tetracycline residue is of great significance for ensuring product quality and protecting human health. Here, a colorimetric/fluorescent dual-mode biosensor was developed for the detection of tetracycline in honey by using metalloporphyrin [TCPP(Fe)] covalently modified NH 2 -MIL-101(Fe) [named NH 2 -MIL-101(Fe)@TCPP(Fe)]. The morphology, chemical structure and peroxidas-like activity of this hybrid nanozyme were comprehensively studied. Based on excellent catalytic activity and intrinsic fluorescence of NH 2 -MIL-101(Fe)@TCPP(Fe), a colorimetric/fluorescent dual-mode biosensor was developed for the detection of tetracycline. The primary mechanism for this dual mode biosensor was the inhibitory effect of tetracycline on on NH 2 -MIL-101(Fe)@TCPP(Fe) catalyzed chromatic reaction between H 2 O 2 and 3,3′,5,5′-tetramethylbenzidine (TMB)/ o -phenylenediamine (OPD), which was ascribed to the consumption of · OH by tetracycline and the adsorption of tetracycline on the surface of NH 2 -MIL-101(Fe)@TCPP(Fe). After effective validation, this colorimetric/fluorescent dual mode method was applied to detect tetracycline residues in three actual honey samples.
研究领域:
生物化学
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分析化学
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食品安全检测
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2018
·
IF 5.091
Wendong Zhu,Maoqiang Chi,Mu Gao,Ce Wang,Xiaofeng Lu
Synergistic effects play a crucial role in improving the catalytic activity of enzyme-like reactions. The preparation of hybrid nanomaterials for enzyme mimicking that display synergistic enhanced catalytic activity remains a formidable challenge. Titanium dioxide (TiO 2 )/molybdenum disulfide (MoS 2 ) core-shell hybrid nanofibers were synthesized as efficient peroxidase mimics via a three-step approach involving electrospinning, calcination, and hydrothermal treatment. The resulting TiO 2 /MoS 2 hybrid nanofibers exhibited synergistically enhanced peroxidase-like catalytic activity relative to the TiO 2 nanofibers or MoS 2 nanosheets alone. Based on the high peroxidase-like activity of the TiO 2 /MoS 2 hybrid nanofibers, a simple colorimetric approach for the detection of l -glutathione (GSH) was developed, with a detection limit as low as 0.05 μM. This study provides a simple and sensitive sensing platform for the detection of GSH, with prospective applications in colorimetric sensing, environmental monitoring, and medical diagnosis.
研究领域:
材料科学
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生物医学工程
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分析化学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 1
·1-10
·
IF 5.833
Zhu Junlun,Luo Guan,Xi Xiaoxue,Wang Yijia,Selvaraj Jonathan Nimal,Wen Wei,Zhang Xiuhua,Wang Shengfu
A Cu 2+ -modified carboxylated hollow carbon nanospheres (Cu 2+ -HCNSs-COOH) was designed with enhanced peroxidase-like activity for the detection of hydrogen peroxide (H 2 O 2 ) and degradation of methylene blue (MB). Hollow polymer nanospheres were fabricated from aniline, pyrrole, Triton-100, and ammonium persulfate via confined interfacial copolymerization reaction, which can be pyrolyzed to create HCNSs with the hollow gap diameter of about 20 nm under high temperature. Combining the synergistic effect of coordination and electrostatic interaction, Cu 2+ -HCNSs-COOH was constructed by anchoring Cu 2+ on the surface of HCNSs-COOH. Furthermore, Cu 2+ -HCNSs-COOH has higher affinity for 3,3′,5,5′-tetramethylbenzidine and H 2 O 2 of 0.20 mM and 0.88 mM, respectively. Based on the rapid response of Cu 2+ -HCNSs-COOH to H 2 O 2 , we constructed a colorimetric sensing platform by detecting the absorbance of the 3,3′,5,5′-tetramethylbenzidine-H 2 O 2 system at 652 nm for quantifying H 2 O 2 , which holds good linear relationship between 1 and 150 μM and has a detection limit of 0.61 μM. We also investigated the degradation of MB in the presence of Cu 2+ -HCNSs-COOH and H 2 O 2 , which can degrade 80.7% pollutants within 30 min. This research developed an unusual nanozyme for bioassays and water pollution treatment, which broadened the way for the rapid development of clinical diagnostics and water pollution treatment. Graphical abstract
研究领域:
纳米材料科学
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生物分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2020
·Vol. 25
·期 19
·4432
·
IF 3.267
Quan Pan,Yuelin Kong,Kuan Chen,Mi Mao,Xiaohui Wan,Xiaoyan She,Qingsong Gao,Yu He,Gongwu Song
In this work, we report the synthesis of Cu-Ag bimetallic nanopartiles and g-C3N4nanosheets decorated on zeolitic imidazolate framework-8 (ZIF-8) to form a Cu-Ag/g-C3N4/ZIF hybrid. The hybrid was synthesized and characterized by Transmission electron microscopy (TEM), Fourier transformed infrared (FTIR), the X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). The Cu-Ag/g-C3N4/ZIF hybrid has intrinsic peroxidaselike catalytic activity towards the oxidation of TMB in the presence of H2O2. The situ synthesis of Cu-Ag bimetallic nanopartiles on 2D support such as g-C3N4nanosheets would significantly enhance the peroxidaselike catalytic properties of individual Cu-Ag bimetallic nanopartiles and the g-C3N4nanosheets. After loading of Cu-Ag bimetallic nanopartiles and g-C3N4nanosheets on the ZIF-8, the hybrids exhibited superior peroxidaselike catalytic activity and good recyclability. Then, this method was applied for detecting glucose in human serum, owing the significant potential for detection of metabolites with H2O2-generation reactions.Keywords:Cu-Ag/g-C3N4/ZIF hybrid;nanozyme;colorimetric detection;H2O2;glucose
研究领域:
生物化学
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纳米医学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Advanced Composites and Hybrid Materials
·
2018
·Vol. 1
·期 3
·612-623
·
IF 0
Chen Miaomiao,Zhu Jialong,Yang Baochan,Yao Xiuxiu,Zhu Xixi,Liu Qingyun,Lyu Xintian
In the present study, a facile method was proposed to prepare nickel sulfide (NiS) nanoparticles well-dispersed on the surface of montmorillonite/graphene oxide (MMT/GO). The NiS/MMT/GO nanocomposites were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), fluorescence spectrophotometry, etc. Significantly, the novel NiS/MMT/GO nanocomposites exhibited high peroxidase-like activity toward the typical chromogenic substrate, 3,3′,5,5′-tetramethylbenzidine (TMB). However, glutathione (GSH) could inhibit the peroxidase-like activity of NiS/MMT/GO along with a visible color variation. Based on the color change of the system of nanocomposites-H 2 O 2 -TMB, a novel colorimetric sensor was designed and conveniently used to quantitatively detect H 2 O 2 as well as GSH. The limit of detection (LOD) of H 2 O 2 and GSH is 9.73 and 0.5043 μM, respectively. The proposed H 2 O 2 sensor has potential application in the field of biosensing, food, and environment.
ᅟ
研究领域:
纳米材料
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生物传感器
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分析化学
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产品关联
DOI
TALANTA
·
2012
·
IF 3.794
Wei Chen,Lei Hong,Ai-Lin Liu,Jian-Qing Liu,Xin-Hua Lin,Xing-Hua Xia
As a peroxidase mimic, cupric oxide nanoparticles were found to enhance the chemiluminescence (CL) of luminol-H 2 O 2 system up to 400 folds. The CL spectra and radical scavengers were conducted to investigate the possible CL enhancement mechanism. It was suggested that the enhanced CL could be attributed to the peroxidase-like activity of CuO nanoparticles, which effectively catalyzed the decomposition of hydrogen peroxide into hydroxyl radicals . The effects of the reactant concentrations and some organic compounds were also investigated. The proposed method could be used as a sensitive detection tool for hydrogen peroxide and glucose.
研究领域:
生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 3
·1-10
·
IF 6.408
Xu Yaxin,Li Peipei,Zhu Yu,Tang Ying,Chen Haoyu,Zhu Xiaohua,Wu Cuiyan,Zhang Youyu,Liu Meiling,Yao Shouzhuo
A novel nanocomposite of CuO nanoparticle-modified Zr-MOF (CuO/UiO-66) was synthesized and developed as a fluorescence nanoplatform for H 2 O 2 and adenosine triphosphate (ATP) via the “turn-on–off” mode in the presence of terephthalic acid (TA). The structure of CuO/UiO-66 was thoroughly characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), and other techniques. The CuO/UiO-66 with enhanced peroxidase-like (POD) activity obtained due to the Zr 4+ in UiO-66 is beneficial to the aggregation of CuO NPs on its surface. As a result, the strengthened fluorescence at 425 nm with the excitation of 300 nm was found due to the highly fluorescent species of TAOH. This is produced by the oxidation of TA by ·OH that came from the catalysis of H 2 O 2 via the peroxidase mimic of CuO/UiO-66. Hence the modification of CuO NPs on porous UiO-66 can provide a friendly and sensitive physiological condition for H 2 O 2 detection. However, upon addition of ATP, the fluorescence intensity of TAOH at 425 nm effectively declined owing to the formation of complexation of Zr 4+ -ATP and the interaction of CuO to ATP which hampers the catalytic reaction of CuO/UiO-66 to H 2 O 2 . The specific interaction induced “inhibition of the peroxide-like activity” endows the sensitive and selective recognition of ATP. The detection limits were 16.87 ± 0.2 nM and 0.82 ± 0.1 nM, and linear analytical ranges were 0.02–100 μM and 0.002–30 μM for H 2 O 2 and ATP, respectively. The novel strategy was successfully applied to H 2 O 2 and ATP determination in serum samples with recoveries of 97.2–103.8% for H 2 O 2 and 97.6–101.7% for ATP, enriching the avenue to design functional MOFs and providing new avenue of multicomponent bioanalysis. Graphical abstract
研究领域:
分析化学
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生物医学工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 44
·28349-28358
·
IF 4.036
Xin Zhang,Siqin Wang,Jiahao Dao,Jiajing Guo,Yanfang Gao
A novel colorimetric platform based on nano-composites of two-dimensional (2D) molybdenum disulfide nanosheets (MoS2 NSs) and one-dimensional (1D) carbon nanotubes (CNTs), called 2D–1D MoS2-CNT nanozyme, was fabricated for the selective and sensitive determination of hydrogen peroxide (H2O2) in soda water. The MoS2-CNT nanozyme was synthesized through a one-step solvothermal reduction method. The introduced CNTs could effectively prevent the stacking of MoS2 nanosheets (NSs) and not only expanded the interlayer distance of MoS2 NSs from 0.620 nm to 0.710 nm but also improved their specific surface. Under acidic conditions, the as-prepared 2D–1D MoS2-CNT nanozymes could oxidize the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue-oxidized TMB (oxTMB) in the presence of H2O2, resulting in enhanced peroxidase-like (POD-like) activity. The kinetic study showed that MoS2-CNT nanozyme had stronger catalytic activity than natural horseradish peroxidase (HRP). The linear range for H2O2 colorimetric determination was 5.00–500 μmol L−1 with a limit of detection (LOD) of 1.40 μmol L−1. Furthermore, the established determination method was applied to actual samples and the recoveries of H2O2 spiked in soda water were in the range of 92.3–107%, showing feasibility for the analysis of food.
研究领域:
分析化学
·
纳米材料
·
食品分析
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Nian Jiang,Ling Qian,Qiang Peng,Shuqi Zhang,Wanqing Yue
Hydrogen peroxide (H 2 O 2 ) is produced in living organisms and is involved in a variety of redox-regulated processes. Therefore, the detection of H 2 O 2 is important for tracing the molecular mechanisms of some biological events. Here, we demonstrated for the first time the peroxidase activity of PtS 2 -PEG NSs under the physiological conditions. PtS 2 NSs were synthesized by mechanical exfoliation followed by functionalization with polyethylene glycol amines (PEG-NH 2 ) to improve their biocompatibility and physiological stability. Fluorescence was generated by catalyzing the oxidation of o-phenylenediamine (OPD) by H 2 O 2 in the presence of the PtS 2 NSs. The proposed sensor had a limit of detection (LOD) of 248 nM and a detection range of 0.5–50 μM in the solution state, which was better than or comparable to previous reports in the literature. The developed sensor was further applied for the detection of H 2 O 2 released from cells as well as for imaging studies. The results show that the sensor is promising for future applications in clinical analysis and pathophysiology.
研究领域:
生物化学
·
医学诊断
·
材料科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）