JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Simin Liu,Jiamei Liu,Linguo Zhao,Jianjun Pei
Glycosylation, one of the most common and significant modifications in nature, has prompted the development of a cellobiose phosphorolysis route for glycosylation in vivo. However, the process of glycosylation is hampered by the notably low conversion rate of cellobiose. In this work, regulation of the carbon source supply by changing the ratio of glucose to cellobiose improved the conversion rate of cellobiose, resulting in enhancing the efficiency of glycosylation and the production of vitexin. Moreover, three genes (pgm, agp, and ushA) involved in the degradation of UDP-glucose were knocked out to relieve the degradation and diversion of the cellobiose phosphorolysis route. Finally, through the optimization of conversion conditions, we observed a continuous enhancement in cellobiose conversion rate and vitexin production in BL21ΔushAΔagp-TcCGT-CepA, corresponding to an increased concentration of added glucose. The maximum production of vitexin reached 2228 mg/L with the addition of 2 g/L cellobiose and 6 g/L glucose, which was 312% of that in BL21-TcCGT-CepA with the addition of 2 g/L cellobiose. The conversion rate of cellobiose in BL21ΔushAΔagp-TcCGT-CepA reached 88%, which was the highest conversion rate of cellobiose to date. Therefore, this study presents a cost-effective and efficient method to enhance the conversion rate of cellobiose during the glycosylation process.
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Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Jiguang Huang,Haiping Chen,Run Qi,Jihao Yang,Zhaohao Li,Heng Zhang
Coal fly ash (CFA) is a substitute material for ceramic membrane which can effectively reduce the preparation cost. However, CFA based ceramic membranes usually show insufficient mechanical strength for application. Revealing pore-forming mechanisms of different pore-forming agents in membranes preparation is of great significance to improve mechanical strength without harming permeability. In this work, three different pore-forming agents, carbon powder, dolomite and dextrin, were adopted to prepare CFA membranes. Characters including porosity, pore size distribution and permeability of membranes were comparatively analyzed. Effects of pore-forming agents on membranes were discussed. The membrane with dextrin at 1200 °C sintering showed the best comprehensive properties. Its porosity, flexural strength and Darcian permeability coefficient were 39.46 %, 35.56 MPa and 3.84 × 10 −14 m 2 , respectively. Application in oil-in-water emulsion separation indicated higher than 94 % oil rejection. This research provides experimental bases for improving performances of low-cost CFA membranes, thereby conducive to engineering applications.
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CHEMICAL ENGINEERING RESEARCH & DESIGN
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2023
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IF 3.9
Jiguang Huang,Haiping Chen,Jihao Yang,Ziwei Du,Heng Zhang,Zhaohao Li
Transport membrane condenser can efficiently recover water and heat from wet flue gas . Ceramic membrane is the heat and mass transfer medium in the process. However, the relationship between membrane property and the recovery performance is only partially reported based on current research state. This work focuses on the effects of permeability, material, and ceramic particle size of membrane on the water and heat recovery. Membranes were prepared from three different sizes of coal fly ash and an alumina powder. Water and heat recovery performances of the membranes are experimentally compared. Results indicate that increasing membrane permeability improves water and heat recovery because the resistance of heat and mass transfer is reduced. The alumina membrane performs 3–10% more water recovery than the coal fly ash-based membrane while thermal conductivity of the alumina membrane was 11.5 times that of the coal fly ash-based membrane. Increasing ceramic particle size shows two opposite effects on the water and heat recovery, promotion because of the permeability rising, and undermine because of the thermal conductivity reduction. Low-cost membranes with high permeability and high thermal conductivity are recommended in transport membrane condenser application.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Lumin Yang,Xiaoduo Zhao,Yunsong Kong,Renjie Li,Tao Li,Rui Wang,Zhengfeng Ma,Yong-min Liang,Shuanhong Ma,Feng Zhou
The typical symptoms of arthritis are inflammation and lubrication deficiency in joints, which increase wear of articular cartilage along with pain of patients. In the present study, one kind of novel macromolecular/microsphere-based injectable hydrogels (CMC-ODex NPs) with dual functionalities of drug release and lubrication, was fabricated via dynamic Schiff base crosslinking network between carboxymethyl chitosan (CMC) and oxidation dextran nanoparticles (ODex NPs). The CMC-ODex NPs hydrogels exhibited typical viscosity-thinning phenomenon at wide range of shear rates and obvious gel-sol transition feature at specific strain. As a result, CMC-ODex NPs hydrogels presented low friction coefficient at the sliding interface of bovine articular cartilages, resulting from the boundary lubrication of hydrogel and the rolling friction effect of ODex NPs. Furthermore, the anti-inflammatory drug (dexamethasone, DXM) encapsulated in ODex NPs exhibited sustainable drug release behavior during the dynamic shearing process, which making CMC-ODex NPs hydrogels possessed good and stable anti-inflammatory effect. CMC-ODex NPs hydrogels was prepared without utilizing any toxic agents, thus demonstrated excellent cytocompatibility. Our experimental results reveal the CMC-ODex NPs hydrogels is promising to be used as functional lubricant for inhibiting the development of arthritis.
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TALANTA
·
2019
·
IF 4.916
Shuyun Zhu,Xiaolu Yan,Jing Sun,Xian-En Zhao,Xiao Wang
The authors describe a novel method for the determination of artemisinin (ART) by using graphene quantum dots (GQDs) as the fluorescent probes . This method is based on the fact that ART can react with p -aminophenylboronic acid ( p -ABA) to produce p -aminophenol ( p -AP). While in the presence of tyrosinase (TYR), p -AP can be oxidized into 4-amino-1,2-benzoquinone, which effectively quenched the fluorescence of GQDs due to the inner filter effect (IFE). By making use of these reactions, a novel and sensitive fluorescent assay for ART has been developed. The calibration curve for the determination of ART is linear in the range of 0.1–5 μM and 5–55 μM with the detection limit of 33 nM, which is more sensitive than most of other methods. Some common coexisting substances including Ca 2+ , Na + , Mg 2+ , K + , PO 4 3- , starch, lactose, dextrin , and magnesium stearat have negligible effects on the fluorescence intensity of GQDs-TYR- p -ABA system. Finally, the sensing system was successfully applied to the detection of the compound naphthoquine phosphate tablet samples with satisfactory recoveries. This IFE-based GQDs fluorescence sensing strategy is facile and sensitive for the determination of ART because neither the surface modification nor the linking between the receptor and the fluorophore is required.
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ADVANCED FUNCTIONAL MATERIALS
·
2019
·Vol. 29
·期 39
·1904144
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IF 15.621
Zhanwei Zhou,Yadong Liu,Minghua Zhang,Chenzi Li,Ruoxi Yang,Jing Li,Chenggen Qian,Minjie Sun
Protein-based theranostic agents (PBTAs) exhibit superior performance in the diagnosis and therapy of cancers. However, the in vivo applications of PBTA are largely limited by undesired accumulation, penetration, or selectivity. Here, an ATP-supersensitive protein cluster is fabricated for promoting PBTA delivery and enhancing magnetic resonance imaging (MRI)-guided tumor photothermal therapy. Gd 3+ - and CuS-coloaded small bovine serum albumin nanoparticles (GdCuB) are synthesized as the model protein with a size of 9 nm and are encapsulated into charge switchable polycations (DEP) to form DEP/GdCuB nanoclusters of 120 nm. In blood circulation, DEP/GdCuB significantly extends the half-lifetime and thereby enhances the tumor accumulation of GdCuB. When the clusters reach the tumor site, the extracellular adenosine triphosphate (ATP) can effectively trigger the release of GdCuB, resulting in tumoral deep penetration as well as the activation of T 1 -weighted MRI ( r 1 value switched from 2.8 × 10 −3 to 11.8 × 10 −3 m −1 s −1 ). Furthermore, this delivery strategy also improves the tumoral photothermal therapy efficacy with the MRI-guided therapy. The study of ATP-activated nanoclusters develops a novel strategy for tumor deep penetration and on/off imaging of PBTA by size switchable technology, and reveals the potential for MRI-guided therapy of cancers.
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TRANSACTIONS OF THE INDIAN INSTITUTE OF METALS
·
2018
·Vol. 71
·期 8
·1841-1846
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IF 0.91
Shen Liang,Liu Lingyun,Zhu Jinbo,Qiao Erle
Reverse flotation was proposed as a potential method for the upgradation of low rank thermal coal. Higher combustible matter recovery in the float could be obtained using reverse flotation method compared to conventional flotation method. In order to reduce the entrainment of fine particles, oleic acid (OA) was used as froth regulator in coal reverse flotation process. Experimental data showed that the total volume of froth product in the absence of OA was almost 3 times more than that in the presence of 0.45 kg/Mg OA dosage. Reverse flotation results showed that the ash content of froth product increased with the increase of OA dosage which indicated that the entrained coal particles could be decreased by the addition of froth regulator OA.
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NEW JOURNAL OF CHEMISTRY
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2018
·Vol. 42
·期 4
·3097-3101
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IF 3.201
Qiaoling Liu,Nan Zhang,Haoying Shi,Wenyan Ji,Xiaoqian Guo,Wen Yuan,Qin Hu
Highly fluorescent carbon dots (CDs) are prepared by a one-step microwave heating method using citric acid, L-cysteine and dextrin as the precursors. The average size of the CDs is 2.61 nm with good water dispersibility and photostability. Compared with undoped CDs, small amounts of nitrogen (N) and sulfur atom (S) co-doped CDs exhibit a red shift of the absorption spectra to the visible region and lead to a high quantum yield of 22%. Due to the large amount of carboxyl and hydroxyl groups (−COOH and −OH) on the surface of the CDs, Cu2+ is selectively detected in aqueous solution at pH 7.0 with a detection limit as low as 20 nM, which is much lower than the maximum concentration of Cu2+ in drinking water (∼20 μM) permitted by the US Environmental Protection Agency (EPA). Moreover, the application in tap water samples further demonstrates that the as-synthesized CDs are good candidates for the monitoring of Cu2+ in environmental water samples.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Da Huang,Yongming Wang,Chenlan Xu,Minglang Zou,Yangcan Ming,Fang Luo,Zhenjin Xu,Ying Miao,Na Wang,Zhenyu Lin,Zuquan Weng
Curcumin (CUR) is a natural polyphenol that holds promise for treating ulcerative colitis (UC), yet oral administration of CUR exhibits limited bioavailability and existing formulations for oral delivery of CUR often suffer from unsatisfactory loading capacity. This study presents hydroxyethyl starch-curcumin microspheres (HC-MSs) with excellent CUR loading capacity (54.52 %), and the HC-MSs can further encapsulate anti-inflammatory drugs dexamethasone (DEX) to obtain a combination formulation (DHC-MSs) with high DEX loading capacity (19.91 %), for combination therapy of UC. The microspheres were successfully engineered, retaining the anti-oxidative and anti-inflammatory activities of parental CUR and demonstrating excellent biocompatibility and controlled release properties, notably triggered by α-amylase, facilitating targeted drug delivery to inflamed sites. In a mouse UC model induced by dextran sulfate sodium, the microspheres effectively accumulated in inflamed colons and both HC-MSs and DHC-MSs exhibited superior therapeutic efficacy in alleviating UC symptoms compared to free DEX. Moreover, mechanistic exploration uncovered the multifaceted therapeutic mechanisms of these formulations, encompassing anti-inflammatory actions, mitigation of spleen enlargement, and modulation of gut microbiota composition. These findings underscore the potential of HC-MSs and DHC-MSs as promising formulations for UC, with implications for advancing treatment modalities for various inflammatory bowel disorders.
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Frontiers in Microbiology
·
2016
·
IF 4.165
Yang Jie,Wang Guozeng,Ng Tzi Bun,Lin Juan,Ye Xiuyun
Laccases can oxidize a wide range of aromatic compounds and are industrially valuable. Laccases often exist in gene families and may differ from each other in expression and function. Quantitative real-time polymerase chain reaction (qPCR) was used for transcription profiling of eight laccase genes in Cerrena sp. strain HYB07 with validated reference genes. A high laccase activity of 280.0 U/mL was obtained after submerged fermentation for 5 days. Laccase production and laccase gene transcription at different fermentation stages and in response to various environmental cues were revealed. HYB07 laccase activity correlated with transcription levels of its predominantly expressed laccase gene, Lac7. Cu2+ ions were indispensable for efficient laccase production by HYB07, mainly through Lac7 transcription induction, and no aromatic compounds were needed. HYB07 laccase synthesis and biomass accumulation were highest with non-limiting carbon and nitrogen. Glycerol and inorganic nitrogen sources adversely impacted Lac7 transcription, laccase yields, and fungal growth. The present study would further our understanding of transcription regulation of laccase genes, which may in turn facilitate laccase production as well as elucidation of their physiological roles.
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ACS Catalysis
·
2013
·
IF 5.265
Liang Xu,Wei Wei,Hexing Li,Hui Li
In this paper, one-pot dextrin hydrolysis to glucose and the subsequent glucose hydrogenation to sorbitol is successfully conducted by using amyloglucosidase and Ru–B amorphous alloy highly dispersed onto the ordered mesoporous silica encapsulated by a porous silica shell. The porous outer silica shell prevents the larger amyloglucosidase and colloidal hydrolysis substances from contacting Ru–B, which avoids the poisoning effect on each other. Meanwhile, the small glucose can directly access the Ru–B cores through the pores within the silica shells, and the produced sorbitol can readily exit through these pores. Thus, both the amyloglucosidase-aided dextrin hydrolysis and the Ru–B-catalyzed glucose hydrogenation proceed efficiently in bulk solution and inside the chamber, respectively, leading to high sorbitol yield and strong durability. The catalyst design concept used in such a yolk-shell structured configuration opens a new avenue for the development of a highly efficient catalyst system for one-pot cascade reactions containing incompatible parameters.
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ACS Applied Materials & Interfaces
·
2014
·
IF 5.9
Wei Wei,Yu Zhao,Shichao Peng,Haoyang Zhang,Yipeng Bian,Hexing Li,Hui Li
In this paper, we develop a facile strategy for fabricating a yolk–shell structured catalytic system that consists of a core made of Ru supported on mesoporous carbon, which is encaged within a silica shell that has ordered radial mesochannels. A region-selective etching mechanism for the formation of the yolk–shell nanoarchitectures is proposed based on the stronger adsorption ability of the carbon core for etching agent than that of the silica shell for etching agent. By combining such material with amyloglucosidase, one-pot hydrolysis–hydrogenation of dextrin to sorbitol can be conducted, delivering enhanced efficiency and showing great promise for biomass conversion applications.
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