FOOD CHEMISTRY
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2021
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IF 7.514
Yu-Xiao Wang,Yue Xin,Jun-Yi Yin,Xiao-Jun Huang,Jun-Qiao Wang,Jie-Lun Hu,Fang Geng,Shao-Ping Nie
Macrolepiota albuminosa (Berk.) Pegler is abundant in active polysaccharides , but little is known about their structures and solution properties. In this study, water-extracted polysaccharides from M. albuminosa (MAWP) were purified into three fractions with structural heterogeneity, which was attributed to the diversity in molecular weight, monosaccharide composition and linkage patterns, further affecting their solution properties. Methylation and NMR analysis revealed MAWP-60p and MAWP-70 were a 3- O -methylated glucomannogalactan and a previously unreported glucomannogalactan, whereas MAWP-80 was elucidated as a branched galactoglucan. Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration ( R g ) and O -methyl group attached to the backbone. The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.
研究领域:
真菌学
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生物化学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 19
·6654
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IF 4.927
Wang Zhang,Xinjie Liu,Xun Sun,Rongchun Han,Nianjun Yu,Juan Liang,An Zhou
It is generally believed that freshDendrobium officinale(FDO) has more significant pharmacological activity than driedDendrobium officinale(DDO); however, the difference has not been clearly shown. Our study compared their antioxidant properties both in vitro and in vivo, and the molecular weight arrangement and monosaccharide composition of the freshDendrobium officinalepolysaccharides (FDOPs) and the driedDendrobium officinalepolysaccharides (DDOPs) were analyzed by HPLC-GPC and GC-MS. The results showed that the FDO and its polysaccharides had more significant effects on scavenging DPPH, ABTS, and hydroxyl radicals than the DDO. In addition, both the FDO and DDO significantly reduced lipid peroxidation levels and increased the SOD, T-AOC, CAT, and GSH levels in mice with acute liver damage caused by CCl4, while the FDO and its polysaccharides were more effective. Histopathological analysis further verified the protective effect of the Dendrobium polysaccharides on CCl4-induced liver injury. The determination of the polysaccharides revealed that the polysaccharide and mannose contents of the FDO were significantly higher than their dried counterparts, and the homogeneous arrangement of the polysaccharides in the FDO was degraded into three polysaccharide fragments of different molecular weights in the DDO. Overall, our data identified differences in the antioxidant activities of the FDO and DDO, as well as the reasons for these differences.Keywords:Dendrobium officinale;antioxidant activity;polysaccharide;mannose;monosaccharide composition
研究领域:
药理学
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植物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Simin Liu,Jiamei Liu,Linguo Zhao,Jianjun Pei
Glycosylation, one of the most common and significant modifications in nature, has prompted the development of a cellobiose phosphorolysis route for glycosylation in vivo. However, the process of glycosylation is hampered by the notably low conversion rate of cellobiose. In this work, regulation of the carbon source supply by changing the ratio of glucose to cellobiose improved the conversion rate of cellobiose, resulting in enhancing the efficiency of glycosylation and the production of vitexin. Moreover, three genes (pgm, agp, and ushA) involved in the degradation of UDP-glucose were knocked out to relieve the degradation and diversion of the cellobiose phosphorolysis route. Finally, through the optimization of conversion conditions, we observed a continuous enhancement in cellobiose conversion rate and vitexin production in BL21ΔushAΔagp-TcCGT-CepA, corresponding to an increased concentration of added glucose. The maximum production of vitexin reached 2228 mg/L with the addition of 2 g/L cellobiose and 6 g/L glucose, which was 312% of that in BL21-TcCGT-CepA with the addition of 2 g/L cellobiose. The conversion rate of cellobiose in BL21ΔushAΔagp-TcCGT-CepA reached 88%, which was the highest conversion rate of cellobiose to date. Therefore, this study presents a cost-effective and efficient method to enhance the conversion rate of cellobiose during the glycosylation process.
研究领域:
生物化学
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代谢工程
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DOI
PubMed
高影响因子
SYNTHESIS-STUTTGART
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2021
·Vol. 53
·期 14
·2435-2448
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IF 3.157
Xin Zhou,Qing Long,Dongwei Li,Jingru Gao,Qikai Sun,Shaozi Sun,Yong Su,Peng Wang,Wenjie Peng,Ming Li
Structurally defined and pure oligosaccharides corresponding to β-glucans have attracted great attention because of their potential properties as immunostimulating agents and as antigens of vaccine candidates. We herein describe a convergent synthesis of ready-to-conjugate tridecasaccharides composed of a β-1,3-glucan nonasaccharide backbone and a β-1,6-glucan tetrasaccharide branch. The assembly was achieved by employing trichloroacetimidate glycosylations and features the gram-scale preparation of the nonasaccharide backbone and installation of the tetrasaccharide branch involving orthoester rearrangement to the glycoside.
研究领域:
有机化学
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生物化学
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疫苗研究
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DOI
高影响因子
CARBOHYDRATE RESEARCH
·
2024
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IF 2.4
Lan Luo,Lisha Lin,Sheng Huang,Yunxi Zhou,Shengmei Yang,Yan Zhu,Lanyan Zhang,Donghua Xiong,Yongsheng Wu,Mingyi Wu
A sensitive and precise HPLC-DAD method with pre-column PMP derivatization was established and validated, for analyzing the polysaccharides in Bacillus Calmette-Guérin polysaccharide and nucleic acid (BCG-PSN) isolates, after acid hydrolysis. And the HPLC fingerprint profiling was used to analyze its monosaccharide composition. The monosaccharide concentration-peak area calibration curve was of good linearity (R 2 > 0.99), over the range of 0.016–0.08 mg/mL for mannose or 0.24–1.20 mg/mL for glucose, with high recovery of 93–105 % for quality control samples. The intra-day RSD values of mannose and glucose concentration were less than 2.5 % and 2.1 %, respectively, and their inter-day RSD values were less than 4.3 % and 2.2 %, respectively, and remained stable for up to 14 days. This method also remained durable against changes in chromatographic parameters, but it's susceptible to the flow rate of mobile phase. Additionally, the method was applied to analyze the content of mannose and glucose in 22 batches BCG-PSN powder and 17 batches BCG-PSN injection. The results showed that the HPLC-DAD fingerprint spectra of all the BCG-PSN powder and BCG-PSN injection samples had a high degree of similarity, with the similar indexes up to 0.999 and 0.998, respectively. The HPLC-DAD method with pre-column PMP derivatization is highly rapid, effective, visual, and accurate for determination of monosaccharide contents. The validated method was successfully applied to the analysis of polysaccharide in both BCG-PSN powder and injection.
研究领域:
生物化学
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药物分析
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医学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2022
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IF 10.723
Qingxia Yuan,Hong Li,Qian Wang,Shujing Sun,Ziyu Fang,Hao Tang,Xiaohuo Shi,Jing Wen,Lianghua Huang,Meng Bai,Shengping Zhong,Yonghong Liu,Chenghai Gao,Longyan Zhao
Stichopus monotuberculatus is a tropical sea cucumber species and used as a folk medicine and tonic food . In this study, a fucosylated glycosaminoglycan (SmFG), the depolymerized SmFG (dSmFG) and its oligosaccharide fractions were prepared. The SmFG and its depolymerized products were comprised of a chondroitin-sulfate-E backbone, and various sulfated fucose side chains, including an unusual disaccharide side chain connected to the C-3 position of D-glucuronic acid (GlcA) or GlcA-ol. A peeling reaction occurred during the deaminative depolymerization process. The dSmFG and its fractions showed strong anticoagulant activity by selectively inhibiting intrinsic tenase complex, and had no anti-factor IIa, Xa and VIIa activity. The anticoagulant activity reduced with the decrease of molecular weight , and the unusual branch and novel reducing end may enhance the anticoagulant activity. These findings can provide significant information for development and utilization of depolymerized products from SmFG in food and pharmaceutical industries .
研究领域:
海洋生物学
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药理学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
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2019
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IF 3.197
Ping-Ping Wang,Qiang Huang,Chun Chen,Li-Jun You,Rui Hai Liu,Zhi-Gang Luo,Mou-Ming Zhao,Xiong Fu
A novel polysaccharide (MFP4P) was isolated from mulberry fruits ( Morus alba L . ) pulp using a combination of DEAE-Sepharose fast-flow and Sephadex G-100 chromatographic columns. Structural characterization revealed that MFP4P was composed of arabinose, rhamnose, galactose, glucose and galacturonic acid with a molecular weight of 198.2 kDa. The linkage types of MFP4P were proved to be (1 → 6)-linked α-D-Glc, (1 → 2)-linked α-L-Rha, (1 → 3)-linked α-D-Gal, (1 → 3)-linked β-L-Rha and (1 → )-linked α-L-Ara. The MFP4P showed good antioxidant activity with an oxygen radical absorbance capacity value of 121.8 ± 16.5 μM trolox equivalent/g. In addition, the MFP4P promoted pancreatic cell proliferation, increased insulin secretion, and enhanced glucose consumption. In the non-toxic range, the polysaccharide and zinc complexes (MFP4P-Zn) exhibited higher antioxidant and hypoglycemic activities than MFP4P at the same concentration. In vivo hypoglycemic study, MFP4P and MFP4P-Zn both showed decreased blood glucose level and glucose tolerance compared with the model control group.
研究领域:
药理学
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生物化学
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营养科学
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分子生物学
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产品关联
DOI
高影响因子
JOURNAL OF CARBOHYDRATE CHEMISTRY
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2016
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IF 0.738
Faisal Nureldin Awad,Anna Kulinich,Ming Jun Yao,Xu Chu Duan,Zhi Peng Cai,Bin Gu,Li Liu,Josef Voglmeir
GRAPHICAL
研究领域:
生物化学
·
酶学
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产品关联
DOI
高影响因子
JOURNAL OF CARBOHYDRATE CHEMISTRY
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2019
·
IF 0.828
Shomaila Mehmood,Li-Yuan Zhou,Xiao-Fang Wang,Xiao-Du Cheng,Fan-Ju Meng,Ya Wang,Yong-Ming Lu,Yan Chen
A novel heteroglycan TLH-G was successfully isolated from Tricholoma lobayense cultivated in south China. TLH-G was composed of five monosaccharides, namely, mannose, rhamnose, glucuronic acid, glucose and galactose, and consisted of →1)-β-D-Glcp-(6→ and →1)-α-D-Galp-(3→ motifs. TLH-G had excellent scavenging activities towards 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals. Structure-activity relationship analysis demonstrated that glycosidic bond types, monosaccharide composition, and branching degree were more important for the antioxidant activity of polysaccharides from Tricholoma lobayense than other structural factors. All the above results showed that the growth environment played a crucial role in the structure and antioxidant activity of polysaccharides from Tricholoma lobayense.
研究领域:
真菌学
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生物化学
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药理学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2015
·
IF 0.915
Wanxia Xu,Lei Liang,Mingjun Zhu
Fructose, glucose, and sucrose in sugarcane molasses are determined simultaneously by high performance liquid chromatography using maltose as internal standard with refractive index detector. The mixture of the diluted samples and internal standard was purified with Sep-Pak C18 solid-phase extraction and filtered through a 0.22-μm membrane before injection. The results showed that the linear ranges for fructose, glucose, and sucrose were 3.30–16.48, 1.80–9.02, and 5.94–29.70 g/L with the squared correlation coefficients (R2) being 0.9986, 0.9987, and 0.9955, respectively. The method is simple, quantified, and time-saving for determination of sugars in sugarcane molasses.
研究领域:
食品科学
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分析化学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
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IF 3.909
Yirui Wang,Shaowei Han,Ruofei Li,Baosong Cui,Xianjie Ma,Xinzhu Qi,Qi Hou,Mingbao Lin,Jinye Bai,Shuai Li
Two water-soluble polysaccharides , BSP-1 and BSP-2, were extracted and purified from the tuber of Bletilla striata . The molecular weights of BSP-1 and BSP-2 were 83.54 kDa and 12.60 kDa, respectively. The composition and proportion of BSP-1 and BSP-2 were mannose and glucose in molar ratios of 4.0:1.0 and 3.0:1.0 respectively. The two polysaccharides had a linear backbone, consisting mainly of repeating β‑1,4‑linked d ‑mannosyl residues and β‑1,4‑linked d ‑glucosyl residues. BSP-1 exhibited immunomodulatory effects by increasing the thymus and spleen indices of immunocompromised model mice.
研究领域:
植物化学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2022
·
IF 3.571
Qiong Ge,Shan-Shan Zhou,Ni-Na Xie,Ming Kong,Jin-Di Xu,He Zhu,Jing Zhou,Song-Lin Li,Hong Shen
Atractylodis Macrocephalae Rhizoma (AMR) is one of commonly used medicinal and edible herbs in China. It is often sulfur-fumigated during post-harvest processing. Carbohydrates are important active components of AMR. However, it is unknown whether sulfur-fumigation would induce changes on carbohydrates. Here, carbohydrates including polysaccharides , oligosaccharides and free monosaccharides were comprehensively analyzed to characterize the quality changes of sulfur-fumigated AMR. Determination of both homemade sulfur-fumigated AMR samples and commercial samples from market revealed that sulfur-fumigation did not affect molecular weight distribution of polysaccharides , but altered polysaccharides content and its ratios of constituent monosaccharides , especially glucose (Glc) and fructose (Fru), as well as the contents of oligosaccharides DP2–10 and free monosaccharide Fru. Moreover, the variations enhanced with the increasing of residual SO 2 content. The potential transformation mechanisms could be due to the hydrolysis of polysaccharides. The research outcomes could provide a chemical basis for the safety and efficacy evaluations of sulfur-fumigated AMR.
研究领域:
中药学
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植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）