Foods
·
2023
·Vol. 12
·期 18
·3468
·
IF 5.2
Chongzhen Sun,Hongyan Li,Xiaodan Hui,Yurong Ma,Zhina Yin,Qingsong Chen,Cong Chen,Hui Wu,Xiyang Wu
Mulberry leaf protein hydrolysates (HMP), and their in vitro gastrointestinal digests (GHMP), have shown favorable chemical antioxidant activities. The aim of this study is to investigate the potential protective effects of HMP and GHMP against 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress in human erythrocytes. The inhibition rate of hemolysis, the reactive oxygen species (ROS) level, the concentration of malondialdehyde (MDA), the reduced glutathione (GSH) and oxidized glutathione (GSSH), and the enzymatic activities of total superoxide dismutase (SOD), catalase (CAT), and cellular glutathione peroxidase (GSH-Px) were evaluated as the biomarkers of oxidative status in human erythrocytes. The results showed that HMP and GHMP effectively inhibit the occurrence of erythrocyte hemolysis in the range of 0.025–1.0 mg/mL, and the inhibition rates of HMP and GHMP reached 92% and 90% at concentrations of 0.4 mg/mL and 1.0 mg/mL, respectively. HMP and GHMP reduced the AAPH-induced oxidative hemolysis damage via suppressing the generation of ROS by inhibiting the formation of MDA, maintaining the balance of GSH/GSSG, and preserving the activities of the antioxidant enzymes, including SOD, GSH-Px, and CAT. Our findings revealed that both HMP and GHMP could be used as natural antioxidants, and have the potential for further application in the development of functional foods.Keywords:mulberry leaf;digested protein hydrolysates;erythrocytes;oxidative stress;natural antioxidants
研究领域:
营养学
·
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Qiang Li,Yang Liu,Guiliang Li,Qiu Han,Haibo Lin,Shifang Yu,Yanfang Zhang,Fu Liu
The objective of this study is to optimize the storage conditions for red blood cells (RBCs) by reducing levels of metabolic byproducts lactic acid. Herein, we develop a novel mixed matrix microsphere (MMM) for removing lactic acid. A polysulfone porous shell was encapsulating polystyrene ion exchange resins, providing transport channels for lactic acid diffusion and adsorption while protecting red blood cells from intense contact. The MMM exhibited a highly porous surface with exceptional lactic acid adsorption capacity (287.3 mg·g −1 ) and excellent hemocompatibility that inhibits thrombosis and production of inflammatory factors upon long-term contact with blood. Consequently, these microspheres were tested for blood containers storage for over 35 days. The significant reduction in lactic acid levels within the bloodstream helps maintaining an optimal pH environment for normal RBC metabolism and preserves their oxygen-carrying capacity over time without rapid decay. In summary, MMM shows its practicality for improving long-term storage quality of RBCs while mitigating symptoms associated with transfusion-transmitted infectious diseases.
研究领域:
生物医学工程
·
血液学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2015
·Vol. 38
·期 6
·886-893
·
IF 2.737
Juanjuan Pi,Xia Wu,Shitian Yang,Pingyan Zeng,Yifan Feng
A rapid, sensitive, and reliable approach for analyzing five kinds of erythrocyte phospholipids in Sprague–Dawley rats was provided by ultra high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry with MassLynx TM MassFragment. Improving conventional high performance liquid chromatography techniques, ultra high performance liquid chromatography integrated with quadrupole time-of-flight tandem mass spectrometry offers high sensitivity and increased analytical speed by using columns packed with sub-2 μm particles (1.7 μm), which allows a faster separation to be achieved. Through this method, 83 phospholipids were tentatively characterized based on their mass spectra and tandem mass spectra, as well as by matching the in-house formula database within a mass error of 5 ppm, including 40 phosphatidylcholines, 24 phosphatidyl ethanolamines, three phosphatidylinositols, six phosphatidylserines, and ten sphingomyelins. Our present results proved that the established method could be used to qualitatively analyze complex erythrocyte phospholipids in Sprague–Dawley rats and provide a useful data base for pharmacology and phospholipidomics to seek potential biomarkers of disease prediction.
研究领域:
分析化学
·
生物化学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Yi Zhang,Haijie Zhao,Xiaozhen Wang,Hehan Xiao,Yanqing Guan
Microemulsions have attracted great interest in the biomedical field involving drug delivery, skin treatment and immune-adjuvant. In any case, microemulsions administered in vivo will more or less contact blood tissue inevitably, however there is no specialized investigation on the interaction between microemulsions and human blood components at cellular and membrane levels. Herein we investigate the effect of a typical microemulsion formulation (Cremophor EL/ethanol/oleinic acid/water (CEOW-ME)) with different extent dilution on human blood components (red blood cells (RBCs), coagulation factor, plasma albumin) and blood coagulation function. It was found that CEOW-ME had a concentration dependent effect on blood components and displayed anti-coagulant activity, causing coagulation factor activation, platelet aggregation impairment and RBC morphology alteration, whereas no impacting on fibrinogen polymerization. Interestingly CEOW-ME had greater influence on the coagulation factors in the plasma related to intrinsic coagulation pathway than extrinsic coagulation pathway. Additionally, spectroscopy studies (DLS, UV–vis, Fluorescence and CD) demonstrated that the impact of CEOW-ME on the size, local micro-structure and conformation of albumin was mediated by hydrophobic binding-induced unfolding and disordering of hierarchical albumin structure. These findings give more comprehensive information for forecasting the potential interaction of the in vivo -administered microemulsions with the cells and bioactive molecules in the human blood.
研究领域:
药理学
·
生物医学工程
·
血液学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Tong Sun,Xi Guo,Rui Zhong,Lu Ma,Hao Li,Zhipeng Gu,Junwen Guan,Hong Tan,Chao You,Meng Tian
Oligochitosan (OCHI) is known to have some specific biological activities. However, its interactions with blood components and related correlation with molecular structures remains to be clarified due to its growing use in biomedical areas. Herein, a series of OCHI were prepared by hydrogen peroxide induced degradation combined fractionation in ethanol solutions and their molecular structures were characterized by GPC, FTIR, 1 H and 13 C NMR, and then the interactions of the prepared OCHI with blood components, including red blood cells (hemolysis, deformability, and aggregation), coagulation system, complement (C3a, and C5a activation), and platelet (activation, and aggregation), were investigated. For red blood cells, OCHI has a quite low risk of hemolysis in a dose- and MW-dependent manner and the deformability and aggregation were observed in its high MW fraction. The coagulation tests revealed that OCHI is capable of a mild anticoagulation through blocking the intrinsic pathway and the anticoagulation corresponding MW was identified. In terms of complement, OCHI could inhibit C3a in a dose-dependent manner and activate C5a with its high MW fraction. In addition, there is no significant effect of OCHI on platelet activation and aggregation. Based on above results, the interactions related mechanism was discussed and proposed.
研究领域:
生物医学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCONJUGATE CHEMISTRY
·
2017
·
IF 4.818
Lipeng Gao,Hao Wang,Lijuan Nan,Ting Peng,Lei Sun,Jinge Zhou,Ye Xiao,Jing Wang,Jihong Sun,Weiyue Lu,Lin Zhang,Zhiqiang Yan,Lei Yu,Yiting Wang
The application of nano drug delivery systems (NDDSs) may enhance the effectiveness of chemotherapeutic drugs in vivo. However, the short blood circulation time and poor drug release profile in vivo are still two problems with them. Herein, by using red blood cell membrane (RBCm) wrapping and pH sensitive technology, we prepared RBCm wrapped pH sensitive poly(l-γ-glutamylcarbocistein)-paclitaxel (PGSC–PTX) nanoparticles (PGSC–PTX@RBCm NPs), to prolong the circulation time in blood and release PTX timely and adequately in acidic tumor environment. The PGSC–PTX NPs and PGSC–PTX@RBCm NPs showed spherical morphology with average sizes about 50 and 100 nm, respectively. The cytotoxicity of PGSC–PTX@RBCm NPs was considerably decreased compared with that of PGSC–PTX NPs. PTX release from PGSC–PTX and PGSC–PTX@RBCm NPs at pH 6.5 was remarkably higher than those at pH 7.4, respectively. The PGSC–PTX@RBCm NPs exhibited remarkably decreased uptake by macrophages than PGSC–PTX NPs. The area under the curve within 72 h (AUC0–72h) for is significantly higher than PGSC–PTX NPs. The PGSC–PTX@RBCm NPs also showed significantly stronger growth-inhibiting effect on tumor than PGSC–PTX NPs. These results indicated that PGSC–PTX@RBCm NPs have acidic drug release sensitivity, the characteristics of long circulation, and remarkable tumor growth inhibiting effect. This study may provide an effective strategy for improving the antitumor effect of NDDS.
研究领域:
药学
·
肿瘤学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 6
·1159-1167
·
IF 3.286
Ju Yan Jun,Li Na,Liu Shi Gang,Liang Jia Yu,Gao Xin,Fan Yu Zhu,Luo Hong Qun,Li Nian Bing
The Red-emitting nitrogen-doped carbon dots (N-CDs) are synthesized using o-phenylenediamine by a one-step method, and can serve as a fluorescent probe for “turn off” detection of hematin in human red cells. The red-emitting N-CDs can be obtained only in acidic conditions and the emission of the red-emitting N-CDs is pH-dependent, indicating proton-controlled synthesis and emission. The red-emitting N-CDs are 2.7 nm in mean size and have a uniform dispersion and exhibit a high quantum yield (12.8%) and great optical properties. The developed sensing system for hematin displays a linear response from 0.4 to 32 μM with a detection limit of 0.18 μM. Importantly, this fluorescent probe demonstrates a good potential practicability for the quantitative detection of hematin in complex matrixes.
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研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2022
·
IF 6.51
Hengtong Zuo,Jihong Qiang,Yidan Wang,Rongrong Wang,Geng Wang,Liqing Chai,Guolian Ren,Yongdan Zhao,Guoshun Zhang,Shuqiu Zhang
Targeting delivery and prolonging action duration of artemisinin drugs are effective strategies for improving antimalarial treatment outcomes. Here, dihydroartemisinin (DHA) loaded poly (lactic- co -glycolic acid) (PLGA) nanoparticles (PDNs) were prepared and further cloaked with red blood cell (RBC) membranes via electrostatic interactions to yield RBC membrane-cloaked PDNs (RPDNs). The prepared RPDNs displayed a notable “core-shell” structure, with a negative surface charge of −29.2 ± 4.19 mV, a relatively uniform size distribution (86.4 ± 2.54 nm , polydispersity index of 0.179 ± 0.011), an average encapsulation efficiency (70.1 ± 0.79%), and a 24-h sustained-release behavior in vitro . Compared with PDNs, RPDNs showed markedly decreased phagocytic activity by RAW 264.7 cells and had prolonged blood circulation duration. The Pearson correlation coefficient of RPDNs distribution in infected red blood cells (iRBCs) was 0.7173, suggesting that RPDNs could effectively target Plasmodium -iRBCs. In Py By265-infected mice, RPDNs showed a higher inhibition ratio (88.39 ± 2.69%) than PDNs (83.13 ± 2.12%) or DHA (58.74 ± 3.78%), at the same dose of 8.8 μmol/kg. The ED 90 of RPDNs (8.13 ± 0.18 μmol/kg) was substantially lower than that of PDNs (14.48 ± 0.23 μmol/kg) and DHA (17.67 ± 3.38 μmol/kg). Furthermore, no apparent abnormalities were detected in routine blood examination, liver function indexes, and pathological analysis of tissue sections of Py By265-infected mice following RPDNs treatment. In conclusion, the prepared RPDNs exhibited enhanced antimalarial efficacy, prolonged circulation, targeted delivery to Plasmodium -iRBCs, and satisfactory biocompatibility .
研究领域:
药学
·
纳米技术
·
疟疾研究
·
药物递送系统
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Jing Zhan,Qian S. Liu,Yuzhu Zhang,Zhendong Sun,Qunfang Zhou,Guibin Jiang
Silica nanoparticles (SiNPs) have attracted increasing attention for their health effects due to the increased risk of exposure to human bodies via diverse routes. Considering that SiNPs enter the circulatory system and inevitably encounter red blood cells (RBCs), it is necessary to investigate their risk of causing erythrocytotoxicity. In this study, three sizes of SiNPs (SiNP-60, SiNP-120, and SiNP-200) were tested for their effects on mouse RBCs. The results showed that SiNPs could induce hemolysis, morphological changes, and phosphatidylserine (PS) exposure in RBCs in a particulate size-related manner. Further investigations on the underlying mechanism indicated that SiNP-60 exposure increased intracellular reactive oxidative species (ROS) generation and subsequently caused the phosphorylation of p38 and ERK1/2 in RBCs. The addition of antioxidants or inhibitors of mitogen-activated protein kinase (MAPK) signaling significantly attenuated PS exposure in RBCs and ameliorated SiNP-induced erythrocytotoxicity. Moreover, ex vivo assays using platelet-rich plasma (PRP) showed that SiNP-60-induced PS exposure in RBCs could trigger thrombin-dependent platelet activation. The contrary evidence from the assays of PS blockage and thrombin inhibition further confirmed that SiNP-60-induced platelet activation was dependent on PS externalization in RBCs, concomitantly with thrombin formation. These findings revealed the procoagulant and prothrombotic effects of SiNPs through the regulation of PS externalization in RBCs, and may be of great help in bridging the knowledge gap on the potential cardiovascular hazards of particulate silica from both artificial and naturally occurring origins.
研究领域:
纳米技术
·
血液学
·
毒理学
·
心血管科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2021
·Vol. 44
·期 9
·1852-1865
·
IF 3.645
Jingjing Wu,Song Zhang,Jiali Li,Xiaoli Zhong,Guiping Feng,Liangqing Hu,Fan He,Hanjing Cen,Yilu Chen,Yanling He,Huasong Zeng,Xiao Chen,Xiaolan Mo
Low-dose methotrexate is the first-line therapy for juvenile idiopathic arthritis. In vivo, methotrexate is converted into a series of methotrexate polyglutamates whose intracellular levels contribute significantly to its efficacy and toxicity. In this study, a novel high-performance liquid chromatography–tandem mass spectrometry method was developed and validated to simultaneously determine erythrocyte methotrexate polyglutamates using stable isotope-labeled internal standards. Erythrocyte samples were precipitated by perchloric acid and then determined on an XBridge BEH C18 column with an XP vanguard precolumn in 12 min. The mobile phase consisted of 10 nM ammonium acetate (pH 10) and methanol under gradient elution. The detection was carried out in multiple reaction monitoring mode via an electrospray ionization source in positive ionization mode. The calibration curve for each metabolite was linear from 2.0 to 500.0 nmol/L ( r 2 > 0.99). The intraday and interday accuracies were between 93.0 and 107.0%, and the corresponding precisions were between 0.8 and 5.2%. The relative recovery ranged from 82.7 to 105.1%, and the relative matrix effect varied from 96.5 to 104.4%. The erythrocyte metabolites were stable for 30 days at −80°C. This simple and accurate method is applicable to routine monitoring of the concentration of erythrocyte methotrexate polyglutamates in patients to achieve individualized treatment.
研究领域:
药理学
·
分析化学
·
临床医学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2025
·Vol. 15
·期 22
·17781-17794
·
IF 4.6
Jiayu Song,Huanhuan Zhang,Xiaohui Zhang,Meiying Liu,Dan Peng,Yuan Ren,Yan Sun,Yunlan Li
The safe and efficient delivery of chemicals and biologics remains crucial for liver disease therapy. In this study, we developed a targeted drug delivery system utilizing a galactose-modified erythrocyte membrane coating technique and drug liposome nanoparticles, which were further optimized using orthogonal experiments and response surface analysis. The specificity, precision, accuracy, and stability exhibited satisfactory performance in bioanalytical analysis. Specifically, targeting ligands (Gal-DSPE-PEG3400) were efficiently inserted into red blood cell (RBC) membranes using a facile insertion method. When Gal-DSPE-PEG3400-RBC was fused with fenofibrate liposome nanoparticles (FNB-Lip) by co-extrusion, the resulting galactose-modified erythrocyte membrane fusion liposome nanoparticles (Gal-RBC-FNB-Lip) showed long-term stability, excellent biocompatibility, prolonged retention time, and superior liver accumulation and therapeutic efficacy. These qualities make it suitable for effective drug delivery. The findings of this study will provide a fundamental basis for research and development of liver-targeted drugs and offer novel insights into the treatment of clinical liver diseases.
研究领域:
药学
·
生物医学工程
·
肝脏疾病治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
·
IF 3.131
Xiaofei Chen,Liujun Song,Xiang Jiang,Xinya Zhang
A novel morphology structure of red blood cell-like (RBC) particles with protuberances (P-RBC) was prepared through dispersion polymerization process. Based on these particles, a superhydrophobic coating film with strong water adhesion and a superhydrophilic film was fabricated depending on orientation of particles during self-assembly progress. The superhydrophobic coating film with water contact angle (CA) of 163.6° was fabricated when the direction of particle cavity was facing-down. Strong water adhesion was resulted from the larger micro-nano structure of particles. Waterdrop tightly attached to the surface of the coating film even if it was taken upside down. Whilst if the cavity of particles was upward, the coating film was transferred to be superhydrophilic of CA of 3.8° under co-efforts of storage ability of cavities, penetration capacity of channel formed by well-ordered particles, and increased surface roughness . This work gives that anisotropy colloidal particles have potential application with their superior wetting characteristics in many fields, such as material transfer process, waterproof protection and oil-water separation.
研究领域:
材料科学
·
生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）