NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 27
·11704-11709
·
IF 3.288
Chao Fan,Hao Tang,Licheng Wang,Yijing Li,Xusheng Wang,Shuai Wang,Xiaojing Liang
Using N-isopropylacrylamide (NIPAM) as a monomer, a core–shell material was successfully prepared via in situ polymerization on the surface of silica microspheres (named NIPAM@Sil), which acts as a hydrophilic interaction chromatography (HILIC) stationary phase. A method for the in situ polymerization of polymer monomers on a silica surface has been developed. It was shown using scanning electron microscopy (SEM) that NIPAM is evenly distributed on the surface of the silica. The synthesized NIPAM@Sil possesses a polar acylamino group arising from the polymerization of NIPAM. As demonstrated by the hydrophilicity, NIPAM@Sil showed excellent HILIC performance for the separation of hydrophilic compounds. This method provides a simple approach for the preparation of a polymer coated silica core–shell stationary phase.
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高影响因子
MOLECULES
·
2019
·Vol. 24
·期 22
·4119
·
IF 3.06
Zhonghua Dong,Yueyue Sun,Guangwei Wei,Siying Li,Zhongxi Zhao
Cordyceps Sinensis, a traditional Chinese medicine and a healthy food, has been used for the treatment of kidney disease for a long time. The aim of present study was to isolate a nucleoside/nucleobase-rich extract fromCordyceps Sinensis(CS-N), determine the contents of nucleosides and nucleobases, and explore its anti-diabetic nephropathy activity. CS-N was isolated and purified by using microporous resin and glucan columns and the unknown compounds were identified by using HPLC-DAD and LC-MS. The effects of CS-N on the epithelial–mesenchymal transition (EMT), extracellular matrix (ECM) depositions, and the MAPK signaling pathway were evaluated in streptozotocin (STZ)-induced diabetic mice and high glucose (HG)-exposed HK-2 cells. CS-N significantly attenuated the abnormity of renal functional parameters, ameliorated histopathological changes, and inhibited EMT and ECM accumulation by regulating p38/ERK signaling pathways. Our findings indicate that CS-N exerts a therapeutic effect on experimental diabetic renal fibrosis by mitigating the EMT and the subsequent ECM deposition with inhibition of p38 and ERK signaling pathways.Keywords:nucleosides and nucleobases;diabetic nephropathy;HK-2 cells;extracellular matrix;epithelial–mesenchymal transition
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Foods
·
2026
·Vol. 15
·期 18
·3254
·
IF 6
Zhenyu Yuan,Xiaoyu Cao,Qianqian Xu,Kun Lin,Xinyue Du,Hai Yan
Hyperuricemia (HUA) and gout are primarily caused by excessive uric acid production resulting from the catabolism of purine nucleosides, notably inosine and guanosine. In this study, a candidate probiotic strain capable of biodegrading nucleosides (both inosine and guanosine) was successfully isolated fromJiaosuand identified asHeyndrickxia coagulansNJ01 by average nucleotide identity analysis. In vitro assays showed 100% biodegradation of 1.0 g/L of both inosine and guanosine by NJ01 cells and its cell-free extracts within 24 h. HPLC and genomic analyses confirmed that inosine and guanosine were biodegraded by purine-nucleoside phosphorylase into hypoxanthine and guanine, which are terminal metabolites not further degradable by the strain, and the key gene was confirmed by PCR. Furthermore, evaluation of the probiotic properties and safety of NJ01 at both genotypic and phenotypic levels confirmed that this strain is a promising probiotic candidate for further in vivo validation. This study provides a crucial foundation for the potential application ofH. coagulansNJ01, a strain capable of biodegrading uric acid precursors, in the development of functional foods for HUA management.
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ANALYTICAL SCIENCES
·
2023
·
IF 1.6
Lai Liang,Zhang Mengyun,Liu Chusheng,Qu Jiahuan,Xu Dongsheng,Jiang Zhengjin
Rapid and effective separation of nucleotides (NTs) and their derivatives is crucial for studying their physiological functions. In this work, we comprehensively evaluated the separation ability of a zwitterionic hydrophilic monolith, i.e., poly(N,N-dimethyl-N-(3-methacrylamidopropyl)-N-(3-sulfopropyl)ammonium betaine- co –N,N’-methylenebisacrylamide) (poly(SPP- co -MBA)) for NTs analysis, including its selectivity, chemical stability under extremely basic condition and compatibility with hydrophilic interaction liquid chromatography (HILIC) coupled with mass spectrometry (HILIC-MS). The poly(SPP- co -MBA) monolith exhibited excellent chemical stability, as evidenced by the low relative standard deviation of retention time (0.16–1.05%) after 4000 consecutive injections over one month under strong alkaline elution condition (pH 10). After optimizing the separation conditions, including buffer pH and concentration, organic solvent content and column temperature, four nucleoside triphosphates, five nucleoside diphosphates and five nucleoside monophosphates were baseline separated within 7 min. Additionally, the mixtures containing one nucleoside and its corresponding mono-, di-, and triphosphates were baseline separated within only 3 min, respectively. It is good HILIC-MS compatibility was also confirmed by the satisfactory peak shape and high response of nine NTs. Overall, the proposed poly(SPP- co -MBA) monolith exhibited good mechanical stability and compatibility of HILIC-MS, making it a promising technique for NTs analysis. Graphical Abstract
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Wei Jia,Jiying Zhu
ε-Polylysine is a novel food preservative approved by the U.S. Food and Drug Administration (FDA), yet the mechanism of its effect on animal-derived foods remains unclear. Assessment of the effect of preservatives on goat meat products is necessary. Herein, metabolite accumulation and protein expression of ε-polylysine (0.025%, w/w) spiked with goat meat were investigated by nontarget metabolomics and proteomics combined with ultrahigh performance liquid chromatography quadrupole-Orbitrap high-resolution-mass spectrometry (UHPLC-Q-Orbitrap HRMS) in a simulated in vitro digestion model. The amino side chain of ε-polylysine increased the activity of glycine aminotransferase due to its nucleophilic nature, inducing a significant upregulation of l-arginine (0.43–0.72 mg kg–1) and creatine (3.98–6.89 mg kg–1), with an improvement in muscle quality of goat meat. Downregulation of enzyme phenylalanine hydroxylase expression led to upregulation of l-phenylalanine (2.26–3.25 mg kg–1) and l-tyrosine (0.98–1.29 mg kg–1). Collectively, this study first revealed the biochemical mechanism of ε-polylysine in goat meat products, which makes available new prospects for more accurate use of ε-polylysine in animal-derived foods.
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JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 21
·2300090
·
IF 3.1
Qiangqiang Jia,Zufan Yang,Qian Wang,Haishan Yang,Xiaofeng Tang,Hongyang Zhang,Lingling Cao,Gong Zhang
It has been proved that purine metabolites are implicated in various biological syndromes and disorders. Therefore, the realization of panoramic detection of purine metabolites will be of great significance to the pathogenesis of purine metabolic disorders. In the present study, an ultra-high performance liquid chromatography with tandem mass spectrometry method was developed for the comprehensive quantification of purine metabolites in rat plasma. The 17 purine metabolites were separated and quantified in the short running time of 15 min. The proposed method was strictly validated by applying SeraSub solution as a matrix and proved to be linear (R 2 ≥ 0.9944), accurate (the recoveries of all analytes ranged from 85.3% to 103.0%, with relative standard deviation values ≤ 9.3%), and precise (the intra- and inter-day precisions were less than 10.8% and 12.4%, respectively). The method was then successfully applied to the qualification of the endogenous purine metabolites in acute gouty arthritis rats, as well as colchicine and anthocyanin-intervened rats. Results showed that uric acid, xanthine, hypoxanthine, and xanthine were considered the key factors of acute gouty arthritis. The established method and measurement of purines in rat plasma might help the investigation of the action mechanisms between purine disorders and related diseases.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·
IF 4.1
Weiliang Guan,Wenqian Nong,Xiaobo Wei,Renchi Chen,Zhihai Huang,Yuting Ding,Xiaoming Qin,Luyun Cai,Linchun Mao
Background Water-free transportation (WFT), as a novel strategy for express delivery of live shrimp ( Litopenaeus vannamei ), was recently developed. However, air exposure during this transportation arouses a series of abiotic stress to the shrimp. In this work, the influences of WFT stress on glycolysis and lipolysis metabolism and meat quality (umami flavor and drip loss) were investigated in comparison with the conventional water transportation (WT). Results Results showed that type II muscle fibers with the feature of anaerobic metabolism were dominated in shrimp flesh. In addition, the increments of intracellular Ca 2+ was detected in WFT and WT, which then activated the AMP-activated protein kinase (AMPK) pathway, and thereby promoted the consumption of glycogen, accumulation of lactate and lipolysis under the enzymolysis of hexokinase (HK), pyruvate kinase (PK), lactate dehydrogenase (LDH), and adipose triglyceride lipase (ATGL). Glycogen glycolyzed to latate. Meanwhile, ATP degraded along with glycolysis resulting in the generation of ATP-related adenosine phosphates such as inosine monophosphate (IMP) with umami flavor and phosphoric acid. More remarkable ( p < 0.05) physiological changes (except LDH and lactate) were observed in WFT compared to WT. Additionally, fatty acid profile also slightly changed. Conclusions The transport stress induced significant energy metabolism changes of shrimp flesh, and therefore effected the flesh quality. The intensifications of freshness (K value) of shrimp flesh were detected due to ATP degradation, which were more pronounced after WFT. However, the drip loss of shrimp flesh was more significantly increased ( p < 0.05) after WFT compared to WT. This article is protected by copyright. All rights reserved.
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PHARMACOLOGICAL RESEARCH
·
2023
·
IF 9.3
Depeng Li,Meihong Zhang,A.La Teng Zhu La,Zhengtian Lyu,Xin Li,Yuqing Feng,Dan Liu,Yuming Guo,Yongfei Hu
The development of hyperuricemia (HUA) and gout is associated with dysbiosis of the gut microbiota. Quercetin can reduce serum uric acid levels and thus alleviate HUA by modulating the gut microbiota. However, the detailed mechanisms involved in this process are not fully understood. Here, we showed that quercetin significantly reduced the serum uric acid level in a chicken HUA model by altering the chicken cecal microbiota structure and function and increasing the abundance of Lactobacillus aviarius . An L. aviarius strain, CML180, was isolated from the quercetin-treated chicken gut microbiota. Strain characterization indicated that quercetin promoted the growth of L. aviarius CML180 and increased its adhesion, hydrophobicity, and co-aggregation abilities. Gavage of live L. aviarius CML180 to a mouse model of HUA—established by adenosine and potassium oxonate—reduced the serum uric acid level and alleviated HUA. The ability of L. aviarius CML180 to decrease the level of uric acid was due to its degradation of purine nucleosides, which are the precursors for uric acid production. A nucleoside hydrolase gene, nhy69 , was identified from the genome of L. aviarius CML180, and the resulting protein, Nhy69, exhibited strong purine nucleoside-hydrolyzing activity at mesophilic temperature and neutral pH conditions. These findings provide mechanistic insights into the potential of quercetin to treat HUA or gout diseases via a specific gut microbe.
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高影响因子
Genes
·
2023
·Vol. 14
·期 6
·1287
·
IF 3.5
Meng Chu,Yichao Qin,Xiuying Lin,Li Ma,Dehai Deng,Daizhu Lv,Pengcheng Fu,Huan Lin
The coconut (Cocos nuciferaL.) is a commercial crop widely distributed among coastal tropical regions. It provides millions of farmers with food, fuel, cosmetics, folk medicine, and building materials. Among these, oil and palm sugar are representative extracts. However, this unique living species ofCocoshas only been preliminarily studied at molecular levels. Benefiting from the genomic sequence data published in 2017 and 2021, we investigated the transfer RNA (tRNA) modifications and modifying enzymes of the coconut in this survey. An extraction method for the tRNA pool from coconut flesh was built. In total, 33 species of modified nucleosides and 66 homologous genes of modifying enzymes were confirmed using a nucleoside analysis using high-performance liquid chromatography combined with high-resolution mass spectrometry (HPLC-HRMS) and homologous protein sequence alignment. The positions of tRNA modifications, including pseudouridines, were preliminarily mapped using a oligonucleotide analysis, and the features of their modifying enzymes were summarized. Interestingly, we found that the gene encoding the modifying enzyme of 2′-O-ribosyladenosine at the 64th position of tRNA (Ar(p)64) was uniquely overexpressed under high-salinity stress. In contrast, most other tRNA-modifying enzymes were downregulated with mining transcriptomic sequencing data. According to previous physiological studies of Ar(p)64, the coconut appears to enhance the quality control of the translation process when subjected to high-salinity stress. We hope this survey can help advance research on tRNA modification and scientific studies of the coconut, as well as thinking of the safety and nutritional value of naturally modified nucleosides.Keywords:Cocos nucifera;epitranscriptomics;LC-MS/MS;nucleoside misidentification;RNA modification
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高影响因子
TALANTA
·
2023
·
IF 6.1
Hongyuan Wu,Zhuolan Xu,Danni Xiong,Xinguang Qin,Gang Liu,Haizhi Zhang
Two dimensional iron metal-organic framework nanosheet (2D Fe MOF) was facilely synthesized at room temperature by simple stirring of iron salts and terephthalic acid ligand in a mixed solution containing triethylamine. Its morphology and structure were fully characterized by TEM, AFM, XPS and TEM element mapping. Then, its peroxidase-mimicking activity was studied by using H 2 O 2 and 3, 3′, 5, 5′- tetramethylbenzidine as substrate. K m and V max of 2D Fe MOF towards H 2 O 2 were 0.02 mM and 2.08 × 10 −8 M s −1 , respectively. Through the formation of cascade reaction between xanthine oxidase and 2D Fe MOF, a visual method for hypoxanthine (Hx) detection was constructed to evaluate aquatic products freshness. After effective validation, this method presented wide linear range (5.0–500.0 μM), low limit of detection (3.29 μM), satisfied accuracy (recovery of 94.78–99.85%), and good selectivity. By using this method, Hx content in shrimp samples at different storage time were determined.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Qing Gao,Dangqin Jin,Jiaqi Xu,Hao Huang,Huanren Cheng,Huaiguo Xue
Herein, we developed a highly sensitive imprinted electrochemical sensor for the trace detection of ribavirin (RBV) using pyrrole (PYR) and pyrro-1-propionyl-alaninoyl-chitooligosaccharides (PPACO) as bifunctional monomers on Prussian blue-gold nanocomposite films. PPACO had strong molecular effect on RBV molecule and was selected by quantitative calculations. After the deposition of the Prussian blue-gold nanocomposite on a glassy carbon electrode (GCE) surface, a 4-aminothiophenol layer successfully self-assembled on the surface. Subsequently, the molecularly imprinted membrane (MIM) was subjected to electrochemical polymerization on the electrode surface using RBV as the template and PPACO and PYR as the two monomers. After eluting the RBV molecules from the MIM, the fabricated RBV-MIM/Fn-Au-PB/GCE exhibited the specific adsorption of RBV. Under optimal conditions, differential pulse voltammetry (DPV) was used to measure the performance of the synthesized sensor, which exhibited a linear relationship between the decreasing peak current and RBV concentration from 0.015 to 3.5 μM with a low detection limit of 3 nM (S/N = 3). As a proof of concept, RBV-MIM/Fn-Au-PB/GCE was also applied to monitor the RBV content in RBV granules. It showed a satisfactory recovery (96.5–99.2%) with a relative standard deviation of less than 3.5% (n = 5), and thus, we believe it has potential for practical applications.
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JOURNAL OF SEPARATION SCIENCE
·
2019
·Vol. 42
·期 15
·2523-2533
·
IF 2.516
Yuan Yuan,Min Jiang,Hongyang Zhang,Ju Liu,Min Zhang,Ping Hu
We present an ultra high performance liquid chromatography with ultraviolet spectroscopy and quadrupole time-of-flight mass spectrometry method for the simultaneous quantification of ten purines (adenine, hypoxanthine, guanine, xanthine, deoxyadenosine, adenosine, inosine, guanosine, xanthosine, and uric acid) and creatinine in human urine. After chromatographic separation on an ACE Excel 2 AQ column, high abundant creatinine and uric acid and the other low abundant purines were sequentially detected by ultraviolet and quadrupole time-of-flight mass spectrometry within a single run. Method validations including specificity (improved by accurate mass measurement), linearity (correlation coefficients ≥0.9944), limit of quantification (0.002–9.756 µg/mL), intra- and interday precision (relative standard deviations ≤9.1 and 14.0%, respectively), accuracy (relative errors ≤13.1%), extraction recovery (between 90.3 and 109.6%), matrix effect (between 85.3 and 110.5%), and stability (relative errors ≤14.3%) were fully evaluated. This approach was applied to characterize the disordered purine metabolism in acute and chronic gout as an example. Quantitative results (normalized by creatinine) showed that an overproduction of urinary purine precursors might be involved in the gout process. The developed method represents a useful tool to investigate the purine disturbances in gout and other relevant diseases.
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