ANALYTICA CHIMICA ACTA
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2023
·
IF 6.2
Chunfang Wang,Yun Tang,Bihong Zhang,Ziying Zhong,Faqiong Zhao,Baizhao Zeng
In this study, PO 4 3− doped Bi 2 WO 6 (BWO-PO) was prepared by hydrothermal method , and then copolymer of thiophene and thiophene-3-acetic acid (P(Th-T3A)) was chemically deposited on the BWO-PO surface. The introduction of PO 4 3− created point defects , greatly improving the photoelectric catalytic performance of Bi 2 WO 6 ; the copolymer semiconductor could form heterojunction with Bi 2 WO 6 to promote the separation of photo-generated carriers, due to its proper band gap. Furthermore, the copolymer could enhance the light absorption ability and photo-electronic conversion efficiency. Hence, the composite had good photoelectrochemical properties. When it was combined with carcinoembryonic antibody through the interaction of –COOH groups of the copolymer and the end groups of antibody for constructing ITO-based PEC immunosensor, the resulting sensor exhibited superb response to carcinoembryonic antigen (CEA), with a wide linear range of 1 pg/mL−20 ng/mL, and a relatively low detection limit of 0.41 pg/mL. It also showed high anti-interference ability, stability, and simplicity. The sensor has been successfully applied to monitor the concentration of CEA in serum. The sensing strategy can also be applied to the detection of other markers by changing the recognition elements, hence it has good application potential.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Xu Zhang,Xiaotong Wang,Rui Shi,Xiaoku Ran,Xiahong He,Deqiang Dou
Ethnopharmacological relevance Red ginseng (RG), a processed product of ginseng (GS), is a generally used qi -tonifying medicine in Traditional Chinese Medicine (TCM). According to the TCM principle, RG is also generally applied to spleen-deficiency syndrome (SDS) clinically for its warmer property. However, the effective substances and mechanism of RG on SDS have not been well investigated. Aim of the study The aim of this study was to explore the effective substances and their mechanism of RG on SDS. Materials and methods The SDS model was established with a compound factor method involving an irregular diet, excessive fatigue and sennae folium with a bitter-cold property. The medicine of RG was split by multi-mode separation methods and analyzed by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS). The appearance indexes such as body weight, body temperature, swimming endurance, urine output, and water content of fecal were determined. The biochemical indexes such as D-xylose, SP , VIP and AChE in the digestive system, CRH, ACTH, CORT, E, T3, T4, T, E2 and 5-HT in the endocrine system, CS, NCR , IDH1 , COX and Na + -K + -ATPase in the metabolism of substance and energy, cAMP and cGMP in the cyclic nucleotide system were analyzed by Enzyme-linked immunosorbent assay (ELISA) kits and biochemical kits. The serum metabolites were analyzed by UPLC-QTOF/MS. Furthermore, the gut microbiota and short-chain fatty acids (SCFAs) in feces were analyzed by 16S rRNA sequencing and headspace gas chromatography-mass method. Results The pharmacological experiments showed that total saponin fraction (RGTSF), less polar fraction (RGLPF), and polysaccharides faction (RGPSF) significantly modulated the “brain-gut” axis-related indexes (the levels of VIP, AChE, and 5-HT). Besides, RGTSF also significantly modulated the hypothalamic-pituitary-adrenal (HPA) axis-related indexes as well as the substance and energy metabolism-related indexes (the levels of ACTH, CORT, A, Na + -K + -ATPase, COX, NCR and CS). RGPSF also significantly modulated the hypothalamus-pituitary-thyroid (HPT) axis-related indexes (the levels of T3 and T4). Secondly, metabolomics indicated that RGTSF could significantly regulate the abnormal metabolic pathways associated with the development of SDS, which involved steroid hormone biosynthesis, taurine and hypotaurine metabolism, primary bile acid biosynthesis, and amino acid metabolism. Subsequently, the study of gut microbiota indicated that RGLPF could increase the diversities of the gut microbiota and the relative abundance of Firmicutes in rats with SDS, while RGWEF significantly increased the relative abundance of Bacteroidetes . At the genus level, RGLPF could increase the relative abundance of Lactobacillus in rats with SDS and decrease that of Akkermansia . Meanwhile, the water-eluted fraction (RGWEF) showed a stronger regulation in SCFAs. Conclusion It is for the first time that the effective substances of red ginseng on spleen-deficiency syndrome were studied systematically, and the different mechanisms of the RG fractions involved in substance and energy metabolism as well as the “brain-gut” axis were revealed. The present study demonstrated that RGTSF, RGPSF, and RGLPF were the effective substances of red ginseng for ameliorating spleen-deficiency syndrome, indicating that ginsenosides composed of primary and secondary saponins as well as polysaccharides were the main effective substances for red ginseng in ameliorating spleen-deficiency syndrome.
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BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Wanru Li,Haiyan Wei,Nianlu Li,Shunyu Li,Yaru Liu,Ranran Liu,Wei Zou,Peishan Hu,Zhihu Zhang,Cuijuan Wang
Rapid detection of diquat (DQ) is essential in clinical diagnosis and rescue. Here, we developed a fast, simple-yet-practical detection strategy for the reliable identification and quantification of DQ in biological fluids. Based on surface-enhanced Raman spectroscopy (SERS), point-of-care detection was realized under the acidic condition with gold nanoparticles as the substrate. Under optimal experimental conditions, the detection limits of the strategy were 17.5 ppb and 1.99 ppm in human urine and gastric juice, respectively. High specificity and selectivity of the SERS strategy were demonstrated using common pesticides and coexisting biological substances. The method was also used to detect biofluids from 5 patients and urine samples from 10 healthy volunteers . The results were in high agreement with spectrophotometric and clinical liquid chromatography-mass spectrometry methods. The volume of urine samples required for this technique is merely 20 μL, and no preparation of the samples is required. Compared to traditional methods used in clinical settings, SERS-based methods are capable of real-time measurements that accurately provide rapid detection and response in non-laboratory settings, with great potential for on-site and point-of-care testing.
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TALANTA
·
2023
·
IF 6.1
Jingyu Xiao,Yanxia Chen,Tailin Xu,Xueji Zhang
Urinalysis is an effective strategy to non-invasively evaluate human health, and surface-enhanced Raman scattering (SERS) may be a powerful technique for use in detecting analytes in urine. Herein, we report a wearable diaper sensor based on a handheld Raman spectrometer for use in the simple, label-free identification of biomolecules (urea, creatinine, and bilirubin) in urine. The raspberry-shaped Au substrate formed on the surface of an Si wafer provides plasmonic enhancement of the SERS signals, with an excellent uniformity and stability. The SERS sensor combines the advantages of flexibility, portability, and multifunctional detection and may be used in identifying multiple analytes in urine. The sensor exhibits high sensitivities in detecting urea, creatinine, and bilirubin , with respective detection limits of 4.17 × 10 −3 M, 5.90 × 10 −6 M, and 1.38 × 10 −7 M (signal-to-noise ratio = 3). Furthermore, we used the wearable diaper sensor to monitor biomolecules at the diagnostic threshold, facilitating non-invasive diagnosis and medical monitoring of disease-related biomarkers.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Ming Shang,Haiyan Wei,Ge Gao,Nianlu Li,Wei Zou,Ranran Liu,Mengping Zhang,Xiao Meng,Wenwen Chen,Yaxin Sun,Cuijuan Wang
Bromadiolone is the most frequently detected anticoagulant rodenticide in human biofluids, resulting in increased cases of poisoning. Here, we present a portable kit that could realize rapid fingerprint detection of DQ in human biofluids. This strategy was based on surface-enhanced Raman scattering (SERS) and salt-induced liquid-liquid phase separation (SILLPS) using a gold/silver core-shell nanoparticle (Au@Ag NPs) substrate. The kit included a portable Raman spectrometer and five tubes that contained Au@Ag NPs colloid, KI solution, saturated NaCl solution, acetonitrile , and Mg(NO 3 ) 2 solution. This strategy did not require pretreatment for urine samples. For blood samples, a 2-min SILLPS method was proposed with satisfactory recovery, providing unique insights into efficient pretreatment of complex biomatrix. The detection limits for urine and blood were calculated to be 3.21 ppb and 6.86 ppb, respectively. A clear trend could be observed between signal intensity and logarithmic concentration of bromadiolone ranging from 0 to 2 ppm with high reproducibility. Such a portable kit based on SILLPS-SERS can rapidly provide fingerprint information of poison. This simple-yet-practical “Pretreat & Detect” SERS kit may open up new opportunities for point-of-care detection of poisons and has great potential as a tool in field applications.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 11
·1-11
·
IF 6.408
Zhou Hang-Yu,Peng Li-Jing,Tian Tao,Zhang Wei-Yi,Chen Guo-Ying,Zhang Hao,Yang Feng-Qing
An effective, selective, and multicolor colorimetric assay for Cu 2+ detection based on the regulation of peroxidase-like nanozyme-mediated etching of gold nanorods (Au NRs) is proposed. Cu 2+ -creatinine complex is selected as the nanozyme that exhibits excellent peroxidase-like activity even in the case of low concentration of Cu 2+ , which can catalyze 3,3,5,5-tetramethylbenzidine (TMB) to produce oxidized TMB (TMB + ) in the presence of hydrogen peroxide, and TMB + is oxidized to generate TMB 2+ after adding H + , and the TMB 2+ can etch Au NRs. The determination of Cu 2+ is achieved based on the blue shift of the longitudinal localized surface plasmon resonance peak of Au NRs. Under the optimal conditions, the developed colorimetric assay exhibits high sensitivity for the detection of Cu 2+ (limit of detection is 0.034 μM) with a wide linear range of 0.05–4.0 μM ( R 2 = 0.987). The solution shows a rainbow-like color in response to the increase of Cu 2+ concentration, which can realize the semi-quantitative detection of Cu 2+ by naked eyes. In addition, the developed method exhibits excellent selectivity for Cu 2+ -detection. The established method was used for the determination of Cu 2+ in lake water, soil, and normal human serum with satisfactory recovery of spiked samples. Graphical abstract
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ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Xi Chen,Qian Huang,Shuyan Ruan,Feili Luo,Ruiyun You,Shangyuan Feng,Lanjin Zhu,Yang Wu,Yudong Lu
Hyaluronidase expression is known to be upregulated in various pathological conditions. A method based on a combination of ratiometric surface-enhanced Raman scattering (SERS) and magnetic separation is described for the determination of hyaluronidase (HAase) activity. Gold nanospheres (AuNPs) functionalized by 4-mercaptophenylboronic acid (4-MPBA) form stable cyclic esters with diol on hyaluronic acid (HA) by the boronic acid group, while Fe 3 O 4 @ [email protected] modified with mercaptoethylamine (MEA) was used as a capture substrate to bind to the carboxyl group on the surface of HA, forming the “ [email protected] @HA/Fe 3 O 4 @ [email protected] @MEA” “core-satellite” structure. When HAase is present, HA is enzymatically disrupted, resulting in the destruction of the “core-satellite” structure, the SERS signal of 4-MPBA is subsequently weakened. The gold shell in the substrate protects the 5,5′-Dithio bis-(2-nitrobenzoic acid) (DTNB) from the external environment, which makes it become an ideal internal standard (IS) molecule for subsequent calibration. Under optimal conditions, the I 1075 /I 1324 varied in the range of 10 −3 - 10 U‧mL −1 HAase activity, with a limit of detection (LOD) of 0.32 mU‧mL −1 ，below the level of HAase in normal human body fluids. This method has been successfully applied to the determination of HAase activity in urine and is expected to provide a new method in disease detection, especially in the non-invasive detection of bladder cancer.
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ACS Sensors
·
2022
·
IF 9.618
Kexin Fan,Jiayang Zeng,Chenyu Yang,Gonglei Wang,Kai Lian,Xiuhong Zhou,Yaping Deng,Guozhen Liu
Uric acid (UA) is an important biomarker for many diseases. A sensitive point-of-care (POC) testing platform is designed for the digital quantification of salivary UA based on a colorimetric reaction on an easy-to-build smartphone-assisted microfluidic paper-based analytical device (SμPAD). UA levels are quantified according to the color intensity of Prussian blue on the SμPAD with the aid of a MATLAB code or a smartphone APP. A color correction method is specifically applied to exclude the light effect. Together with the engineering design of SμPADs, the background calibration function with the APP increases the UA sensitivity by 100-fold to reach 0.1 ppm with a linear range of 0.1–200 ppm. The assay time is less than 10 min. SμPADs demonstrate a correlation of 0.97 with a commercial UA kit for the detection of salivary UA in clinical samples. SμPADs provide a sensitive, fast, affordable, and reliable tool for the noninvasive POC quantification of salivary UA for early diagnosis of abnormal UA level-associated health conditions.
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FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Lang Zhang,Xiufang Xia,Fangda Sun,Haotian Liu,Qian Chen,Baohua Kong
This study aims to investigate the inhibitory effects of hydrocolloids on heterocyclic aromatic amine (HAA) formation in chemical model systems and smoked chicken drumsticks. In chemical model systems I and II, xanthan gum (XG) and carboxymethyl cellulose sodium (CMC-Na) effectively inhibited the 2-amino-1-methyl-6-phenylimidazo[4,5- b ]pyridine (PhIP), 1-methyl-9H-pyrido[3,4- b ]indole (Harman), and 9H-pyrido[3,4- b ]indole (Norharman) formation in comparison with the control sample ( P < 0.05). Arabic gum (AG) inhibited the PhIP and Harman formation ( P < 0.05), but its inhibitory action against Norharman formation was not significant ( P > 0.05). Further mechanism investigation of chemical model system I showed that the formation of PhIP was mainly inhibited via trapping phenylalanine and creatinine by glyoxal and methyglyoxal. A mechanistic investigation of chemical model system II suggested the inhibition of Harman and Norharman formation by XG and CMC-Na could be through trapping tryptophan by glyoxal and methyglyoxal. In smoked chicken drumsticks, CMC-Na had a dose-dependent inhibitory effect against the PhIP, Harman, and Norharman formation in comparison with the control sample ( P < 0.05), and 2.0% CMC-Na had the highest inhibitory rates of 45.83%, 21.82%, and 30.92%, respectively. Moreover, CMC-Na effectively inhibited the formation of PhIP, Harman, and Norharman precursors and intermediates in smoked chicken drumsticks, which further confirmed the above inhibition mechanism of CMC-Na. These results could provide an effective approach to reduce HAA formation in smoked meat products by incorporating hydrocolloids.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Lang Zhang,Yingying Hu,Qian Liu,Qian Chen,Xiufang Xia,Baohua Kong
This study aims to evaluate the heterocyclic aromatic amine (HAA) formation inhibited by cyanidin and rutin in chemical modeling systems and smoked chicken drumsticks. In the PhIP and β-carboline chemical modeling systems, cyanidin inhibited the PhIP, Harman, and Norharman formation while rutin inhibited the PhIP formation but promoted the Harman and Norharman formation compared with control sample ( P < 0.05). A mechanistic investigation confirmed that inhibiting the PhIP formation by cyanidin was mainly through trapping phenylalanine, creatine, creatinine, glucose, phenylacetaldehyde, and an aldol condensation product. In the smoked chicken drumsticks, cyanidin inhibited the PhIP, Harman, and Norharman formation dose-dependently compared to the control sample ( P < 0.05), with 2.0 % (w/v) cyanidin having the highest inhibitory effect. Moreover, cyanidin inhibited the formation of PhIP precursors and intermediates in the smoked chicken drumsticks. These results provide evidence for using phenolic compounds to reduce HAA formation in smoked meat products.
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TALANTA
·
2022
·
IF 6.556
Yi Xiao,Haomin Yi,Guofang Wang,Suhua Chen,Xiang Li,Qinyu Wu,Siyi Zhang,Kexin Deng,Yisheng He,Xiaoping Yang
Pyrrolizidine alkaloids (PAs) and PA N -oxides are hepatotoxic natural products , produced by over 6000 plant species worldwide. However, an unmet need remains for confirmative measurement of PAs in routine clinical tests. Here, we develop a visual, easy-to-use, and economic mesoporous silica-electrochemiluminescence (MPS-ECL) sensor for point-of-care (POC) testing of PAs, utilizing MPS's amplification effect on positive ions . The relationship between PAs' different structures and corresponding Ru(bpy) 3 2+ ECL activity shows that reaction mechanism, stability of intermediate, molecular geometry and alternative anodic reactivity significantly affect the ECL activity. The ECL intensity varies among different PAs: monocrotaline ˃ senecionine N -oxide ˃ retrorsine ˃ senkirkine. The POC sensors possess excellent linearity (0.9993 > R 2 > 0.9944), low detection limits (0.02 μM–0.07 μM), and good recoveries (90.12%–105.93%), indicating good accuracy and practicability. The portable and low-cost sensor is user-friendly, which holds promise to be applied to POC testing of PAs in drugs, food products, and clinical samples, which is promising for initial assessments of PA-induced health risk.
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Journal of Materials Chemistry C
·
2022
·Vol. 10
·期 19
·7633-7640
·
IF 8.067
Haifeng Sha,Bing Yan
As the basic units of proteins, the importance of amino acids in organisms is self-evident and therefore detection of the concentration of amino acids is significant in monitoring human health. Herein, a dual-responsive Eu3+-functionalized metal–organic framework (MOFs) luminescent film (1@SA) was designed and prepared, which not only realizes the simultaneous detection of aspartic acid (Asp) and arginine (Arg) but also has the possibility of being applied in anti-counterfeiting. A portable film sensor is obtained through the cross-linking and solidification of Eu3+ ions and sodium alginate (SA) hydrogel containing uniformly dispersed MOFs. The MOF used in this work is a zinc metal–organic framework (ZnMOF) comprising uncoordinated carboxyl groups that can provide binding sites for Eu3+ ions. Moreover, it is found that the sensor shows unique fluorescence changes under the stimulation of an acidic environment. Therefore, the quantitative detection of Asp and Arg by 1@SA can be achieved, and optical anti-counterfeiting platforms are also designed according to the diversity of fluorescence changes. The new strategy proposed in this work may broaden the scope of lanthanide fluorescence films used in optical fields.
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