Theranostics
·
2019
·
IF 8.063
Huijuan Yu,Wen-Chao Geng,Zhe Zheng,Jie Gao,Dong-Sheng Guo,Yuefei Wang
Detection and quantification of trimethylamine N -oxide (TMAO), a metabolite from gut microbial, is important for the disease diagnosis such as atherosclerosis, thrombosis and colorectal cancer. In this study, a novel method was established for the sensing and quantitative detection of TMAO via molecular recognition of guanidinium-modified calixarene from complex matrix. Methods : Various macrocycles were tested for their abilities to serve as an artificial TMAO receptor. Using the optimized receptor, we developed an indicator displacement assay (IDA) for the facile fluorescence detection of TMAO. The quantification of TMAO was accomplished by the established calibration line after excluding the interference from the various interfering substances in artificial urine. Results : Among various macrocycles, water-soluble guanidinium-modified calix[5]arene (GC5A), which binds TMAO in submicromolar-level, was identified as the optimal artificial receptor for TMAO. With the aid of the GC5A•Fl (fluorescein) reporter pair, TMAO fluorescence “switch-on” sensing was achieved by IDA. The fluorescence intensity increased linearly with the elevated TMAO concentration. The detection was not significantly interfered by the various interfering substances. TMAO concentration in artificial urine was quantified using a calibration line with a detection limit of 28.88 ± 1.59 µM, within the biologically relevant low µM range. Furthermore, the GC5A•Fl reporter pair was successfully applied in analyzing human urine samples, by which a significant difference in fluorescence response was observed between the [normal + TMAO] and normal group. Conclusion: The proposed supramolecular approach provides a facile, low-cost and sensitive method for TMAO detection, which shows promise for tracking TMAO excretion in urine and studying chronic disease progression in humans.
研究领域:
分析化学
·
生物医学工程
·
分子生物学
·
临床化学
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产品关联
DOI
PubMed
高影响因子
Micro & Nano Letters
·
2019
·Vol. 14
·期 8
·892-896
·
IF 0.975
Jiaqi You,Long Ma,Yu He,Yili Ge,Gongwu Song,Jiangang Zhou
This work has assembled ZnSe:Mn/ZnS quantum dots (QDs)-monoclonal anti-microcystin-Leucine Arginine (anti-MC-LR) antibody bioconjugates as a room temperature phosphorescence (RTP) probe using 1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide/N-Hydroxysuccinimide chemistry for detecting MC-LR. Once the specific interactions occurred between these MCs and their antibodies on the surface of ZnSe:Mn/ZnS QDs, the RTP of ZnSe:Mn/ZnS QDs was quenched with the increased concentration of MC-LR. Under the optimised conditions, the linear range of MC-LR was determined to be 0.9–8.8 nM. The linear relationship between ( P 0 – P )/ P 0 and concentration of MC-LR were observed with the regression equation ( P 0 – P )/ P 0 = 0.03908C + 0.0456 with the detection limit of 0.038 nM. The developed RTP immunoassay favoured environmental applications since the interference from autofluorescence and scattering light was greatly eliminated.
研究领域:
分析化学
·
环境科学
·
纳米技术
·
毒理学
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产品关联
DOI
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6.8
Zhongyuan Cai,Lingling Jiang,Yingzi Cao,Shengxiang Fu,Shasha Wang,Yuting Jiang,Haojie Gu,Na Li,Xiaomin Fu,Shimin Tang,Jiang Zhu,Weidong Cao,Lei Zhong,Zhuzhong Cheng,Chunchao Xia,Su Lui,Bin Song,Qiyong Gong,Hua Ai
Effective vascular and hepatic enhancement and better safety are the key drivers for exploring gadolinium-free hepatobiliary contrast agents. Herein, a facile strategy proposes that the high lipophilicity may be favorable to enhancing sequentially vascular and hepatobiliary signal intensity based on the structure–activity relationship that both hepatic uptake and interaction with serum albumins partly depend on lipophilicity. Therefore, 11 newly synthesized derivatives of manganese o-phenylenediamine-N,N,N′,N′-tetraacetic acid (MnLs) were evaluated as vascular and hepatobiliary agents. The maximum signal intensities of the heart, liver, and kidneys were strongly correlated with log P, a key indicator of lipophilicity. The most lipophilic agent, MnL6, showed favorable relaxivity when binding with serum albumin, good vascular enhancement, rapid excretion, and reliable hepatobiliary phases comparable to a classic hepatobiliary agent, gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) for in vivo liver tumor imaging. Inhibition experiments confirmed the hepatic targeting of MnL6 is mediated by organic anion-transporting polypeptides.
研究领域:
医学成像
·
药物化学
·
放射学
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DOI
PubMed
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2022
·
IF 3.322
Chunxia Shi,Maohua Pei,Yao Wang,Qian Chen,Pan Cao,Luyi Zhang,Jin Guo,Wei Deng,Luwen Wang,Xun Li,Zuojiong Gong
Evidence shows that trimethylamine (TMA)/trimethylamine-N-oxide (TMAO) is closely related to non-alcoholic fatty liver disease (NAFLD). The conversion of TMA to TMAO is mainly catalyzed by flavin-containing monooxygenases 3 (FMO3) and FMO1. In this study, we explored the role of TMA in the process of NAFLD. The human NAFLD liver puncture data set GSE89632 and rat TMAO gene chip GSE135856 was downloaded for gene differential expression analysis. Besides, oleic acid (OA) combined with palmitate were used to establish high-fat cell model. TMA, TMAO and FMO1-siRNA were used to stimulate L02 cells. Contents of free fatty acid (FFA), triglyceride (TG), TMAO, FMO1 and unfolded protein response (UPR) related proteins GRP78, XBP1 , Derlin-1 were detected. Our results showed that FMO1 and PEG10 were important in the progression of NAFLD. Immunohistochemistry showed that FMO1 in NAFLD liver was increased. In addition, the contents of FFA, TG, FMO1 expression, and TMAO were significantly increased after OA + palmitate and TMA stimulation. However, after silencing FMO1 with siRNA, the expressions of these molecules were decreased. Besides, the protein levels of GRP78, XBP1, Derlin-1 were increased after TMAO treatment (all P < 0.05). In Conclusion, high fat and TMA could induce the expression of FMO1 and its metabolite TMAO. When FMO1 is silenced, the effects of high fat and TMA on TMAO are blocked. And the role of TMAO in NAFLD may be through the activation of UPR.
研究领域:
肝病学
·
分子生物学
·
代谢组学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2023
·
IF 6.1
Zouyan HE,Hanyue Zhu,Jianhui Liu,Erika Kwek,Ka Ying Ma,Zhen-Yu Chen
Trimethylamine-N-oxide (TMAO), originating from dietary trimethylamine-containing nutrients such as choline, has been recognized as a risk factor for atherosclerosis. Mangiferin is a bioactive xanthone initially extracted from mango (Mangifera indica). The present study aimed to investigate the effect of mangiferin on TMAO-induced atherogenesis in mice fed a high-choline diet. Female ApoE-/- mice were randomly divided into three groups and fed either a control diet, a high-choline diet with 1% free choline, or an experimental diet with 1% free choline plus 0.5% mangiferin for 15 weeks. Our results showed that a high-choline diet elevated plasma TMAO level, accelerated atherogenesis, promoted cholesterol accumulation, and reduced the generation of short-chain fatty acids (SCFAs) by gut microbes. Mangiferin alleviated inflammation, and lowered plasma total cholesterol level by facilitating the elimination of neutral and acidic sterols in feces, resulting in a 16.7-29.0% reduction in aortic atherosclerotic lesions. Notably, mangiferin could favorably remodel the composition of gut microbiota by fostering the growth of the beneficial taxa Akkermansia, Parabacteroides, and Bifidobacteriaceae, while reducing the relative abundance of the pathogenic genus Helicobacter. This modulation led to a decrease in plasma lipopolysaccharide level, enhanced production of total SCFAs by gut microbes, and reduced susceptibility to atherosclerosis. In conclusion, mangiferin exhibited its ability to alleviate TMAO-induced atherosclerosis through its anti-inflammatory, cholesterol-lowering, and gut microbial modulatory activities.
研究领域:
心血管疾病
·
营养学
·
微生物学
·
药理学
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DOI
PubMed
高影响因子
Scientific Reports
·
2021
·Vol. 11
·期 1
·1-13
·
IF 4.38
Wang Zijing,Ma Peng,Wang Yisa,Hou Biyu,Zhou Can,Tian He,Li Bowen,Shui Guanghou,Yang Xiuying,Qiang Guifen,Yin Chengqian,Du Guanhua
Endoplasmic reticulum (ER) stress is a cellular state that results from the overload of unfolded/misfolded protein in the ER that, if not resolved properly, can lead to cell death. Both acute lung infections and chronic lung diseases have been found related to ER stress. Yet no study has been presented integrating metabolomic and transcriptomic data from total lung in interpreting the pathogenic state of ER stress. Total mouse lungs were used to perform LC–MS and RNA sequencing in relevance to ER stress. Untargeted metabolomics revealed 16 metabolites of aberrant levels with statistical significance while transcriptomics revealed 1593 genes abnormally expressed. Enrichment results demonstrated the injury ER stress inflicted upon lung through the alteration of multiple critical pathways involving energy expenditure, signal transduction, and redox homeostasis. Ultimately, we have presented p-cresol sulfate (PCS) and trimethylamine N-oxide (TMAO) as two potential ER stress biomarkers. Glutathione metabolism stood out in both omics as a notably altered pathway that believed to take important roles in maintaining the redox homeostasis in the cells critical for the development and relief of ER stress, in consistence with the existing reports.
研究领域:
生物学
·
生物化学
·
分子生物学
·
药理学
·
医学研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2021
·Vol. 28
·期 4
·1-9
·
IF 3.097
Zhao Jinnan,Chen Shuo,Zhao Jingbo,Zhang Zhiyuan,Zhang Junying
A new method was provided to synthesize Diels–Alder (DA) self-healing cross-linked polyamides (SH-cPAs) with high tensile strength and good self-healing efficiency. A furfurylamine-N,N’-bis(methyl propionate) (FA-BMP) was synthesized through the Michael addition of furfurylamine and methyl acrylate. Bulk polycondensation of FA-BMP with bis(2-aminopropyl) polypropylene glycol and m -xylylenediamine were conducted, and several polyamide prepolymers containing furan pendent groups (pFU-PAs) were synthesized. From a DA reaction between pFU-PAs and 1,5-bismaleimido-2-methylpentane, different SH-cPAs were prepared. The pFU-PAs were characterized by 1 H NMR, 13 C NMR, FT-IR, amine values, and size exclusion chromatography. The DA reaction in SH-cPAs were demonstrated by FT-IR and 1 H NMR spectra. Their thermally repairing was verified with polarizing optical microscopy and tensile tests. SH-cPAs exhibits tensile strength up to 37 MPa with strain at break of 27%-164%. Their tensile strength after self-repairing was still up to 26 MPa with self-healing efficiency of 70%-92%.
研究领域:
材料科学
·
高分子化学
·
自愈合材料
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·Vol. 416
·期 28
·6621-6638
·
IF 3.8
Chen Chang,Zheng Ting,Chen Yang,Li Zegeng,Wu Huan
The preparation of cellular metabolomics samples and how to achieve comprehensive coverage of different polar metabolites in cell samples in the analysis pose a challenge for cellular metabolomics. In this study, we optimized a metabolomics protocol based on ultra-high-performance liquid chromatography high-resolution mass spectrometry (UPLC/HRMS) for the extraction and detection of metabolites in A549 cells and exploration of the intervention effect of Qi-Yu-San-Long decoction (QYSLD) on A549 cells. The results indicate that the lowest level of ATP leakage was observed when A549 cells were quenched under liquid nitrogen. MeOH/chloroform/H 2 O (1:2:1) extraction yielded more chromatographic peaks and excellent reproducibility, and the relative extraction efficiency of most target metabolites was also high. And we optimized the chromatographic separation conditions in both HILIC and RPLC modes, enabling comprehensive detection and analysis of metabolites with varying polarities. Then, we applied the optimized method to UPLC-Q-TOF/MS-based metabolomics of A549 cells to study the mechanism of QYSLD intervention in non-small cell lung cancer (NSCLC). The CCK-8, EdU staining, and cell cycle assay showed that QYSLD inhibited the proliferation of A549 cells by interfering with the cell cycle and blocking them in the G1 phase. A total of 36 differential metabolites associated with the antitumor effects of QYSLD on NSCLC were identified, mainly involving nicotinate and nicotinamide metabolism, sphingolipid metabolism, and glycerophospholipid metabolism. And western blotting confirmed that the change in 1-methylnicotinamide levels after QYSLD intervention was associated with the inhibition of nicotinamide N-methyltransferase expression in A549 cells. Graphical Abstract
研究领域:
癌症生物学
·
药理学
·
代谢组学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Junjun Chen,Zhenqiang Shi,Xijing Yang,Xiaoyu Zhang,Dongdong Wang,Shengxu Qian,Wenjing Sun,Cunli Wang,Qiongya Li,Zhengjian Wang,Yanling Song,Guangyan Qing
Blood infection can release toxic bacterial lipopolysaccharides (LPSs) into bloodstream, trigger a series of inflammatory reactions, and eventually lead to multiple organ dysfunction, irreversible shock, and even death, which seriously threatens human life and health. Herein, a functional block copolymer with excellent hemocompatibility is proposed to enable broad-spectrum clearance of LPSs from whole blood blindly before pathogen identification, facilitating timely rescue from sepsis. A dipeptide ligand of histidine–histidine (HH) was designed as the LPS binding unit, and poly[(trimethylamine N-oxide)-co-(histidine–histidine)], a functional block copolymer combining the LPS ligand of HH and a zwitterionic antifouling unit of trimethylamine N-oxide (TMAO), was then designed by reversible addition–fragmentation chain transfer (RAFT) polymerization. The functional polymer achieved effective clearance of LPSs from solutions and whole blood in a broad-spectrum manner and had good antifouling and anti-interference properties and hemocompatibility. The proposed functional dihistidine polymer provides a novel strategy for achieving broad-spectrum clearance of LPSs, with potential applications in clinical blood purification.
研究领域:
生物医学工程
·
材料科学
·
药学
·
血液学
·
传染病学
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产品关联
DOI
PubMed
高影响因子
Case Studies in Thermal Engineering
·
2024
·
IF 6.4
Wenjun Zhao,Zhisheng Xu,Long Yan,Guoqing Niu
The research of short-chain fluorocarbon surfactants is crucial in effectively and environmentally extinguishing petrochemical fires. In this study, a short-chain fluorocarbon surfactant named 2 H ,2 H -perfluorooctanoic acid sodium salt (PFH-CA) was synthesized, and then characterized its chemical structure, thermal stability, surface activity, and foam property. Subsequently, PFH-CA was compounded with hydrocarbon surfactants including sodium dodecyl sulfate (SDS), dodecyl trimethyl ammonium chloride (DTAC), and N , N -dimethyldodecylamine- N -oxid (OB-2) at different molar ratios. The surface tension, interaction parameters, and foam property of the PFH-CA/hydrocarbon surfactant compounding systems were investigated. The analysis indicates that PFH-CA exhibits excellent surface activity and thermal stability concomitant with poor foam property. Introducing SDS, DTAC, and OB-2 enhances the foam performance and reduces the consumption of PFH-CA through synergistic interactions. Especially, PFH-CA/DTAC (1:4) system possesses the strongest interactions and the best performance, with critical micelle concentration ( CMC ) and surface tension at CMC ( γ CMC ) of 0.11 mmol/L and 20.90 mN/m, respectively. When concentration of PFH-CA exceeds 0.5 mmol/L, the foaming ability and foam stability stabilize at 30 cm and 90 %, respectively. The enhanced performance of PFH-CA/DTAC system is attributed to the electrostatic attraction between anionic and cationic surfactants, which facilitates the formation of micelles, subsequently leading to better surface activity and foam properties.
研究领域:
化学
·
材料科学
·
消防安全工程
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Zixiang Qu,Lizhen Wang,Shimin Fang,Dawei Qin,Jianhua Zhou,Geng Yang,Hongdong Duan
Hydrogels are attracting carriers for immobilization of small molecules due to their excellent properties, such as low optical background, recyclable and high loading ability. Several advantages will be found in designing optical sensors by utilizing hydrogels, such as the convenience for operation, the easiness to separate from media and the difficulties to introduce impurities to detection environment. As a fluorescein-based sensor for Hg 2+ , the N-(Fluorescein) lactam-N′-methylethylidene ( FLA ) was synthesized, and the structure confirmation process was finished by 1 H NMR, 13 C NMR, FT-IR and mass spectrometry. The interaction of FLA with various mental ions demonstrated the high selectivity to Hg 2+ , which was proved by UV–Vis and fluorescence spectrometry analysis. Meanwhile, the binding mode of FLA Hg 2+ was characterized by FT-IR, 1 H NMR and UV–Vis spectrophotometry . Then, counting FLA as functional monomer and MBA as crosslinker, different proportions of acrylamide (AAm), 2-hydroxyethyl methacrylate (HEMA) and methyl methacrylate (MMA) were copolymerized with FLA and N , N ′-methylenebisacrylamide (MBA) to prepare hydrogel sensors. The hydrogel sensor was characterized by colorimetry and swelling properties. At last, the prepared hydrogels is capable to respond to different colors of Hg 2+ in water or DMSO, and it can be reused after the treatment with ethylenediamine (EDA) solution.
研究领域:
材料科学
·
分析化学
·
环境科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）