CHINESE JOURNAL OF CHEMICAL ENGINEERING
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2021
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IF 3.171
Pascal Habimana,Yanjun Jiang,Jing Gao,Jean Bernard Ndayambaje,Osama M. Darwesh,Jean Pierre Mwizerwa,Xiaobing Zheng,Li Ma
The continuous use of chemical dyes in various industries, and their discharge into industrial effluents, results in severe problems to human life and water pollution. Laccases have the ability to decolorize dyes and toxic chemicals in industrial effluents as green biocatalysts. Their possible industrial applications have been limited by poor reusability, low stability, and loss of free laccase action. In this research, laccase was immobilized on zeolitic imidazolate framework coated multi-walled carbon nanotubes (Laccase@ZIF-8@MWCNTs) via metal affinity adsorption to develop an easy separable and stable enzyme. The optimum reaction conditions for immobilized laccase are at a pH of 3.0 and a temperature of 60 °C. The immobilized laccase was enhanced in storage and thermal stability. The results indicated that Laccase@ZIF-8@MWCNTs still maintained 68% of its original activity after 10 times of repeated use. Most importantly, the biocatalytic system was applied for decolorization of different dyes (20 mg·L −1 ) without a mediator, and up to 97.4% for Eriochrome black T and 95.6% Acid red 88 was achieved in 25 min. Biocatalysts with these properties may be used in a variety of environmental and industrial applications.
研究领域:
环境科学
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生物技术
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材料科学
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产品关联
DOI
高影响因子
Processes
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2023
·Vol. 11
·期 10
·2937
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IF 3.5
Shuyu Yu,Yibo Lu,Dandan Du,Rankun Wu,Xiang Ji,Hao Li
As the largest group of synthetic dyes, azo dyes can pose various health and environmental risks due to their widespread use and challenging degradation. Laccases are efficient green biocatalysts for the degradation of organic pollutants. Herein, we report thein situpackaging of laccase in copper-doped zeolitic imidazolate framework-8 (ZIF-8) for the decolorization of reactive black 5, which is a model azo dye. The immobilization support (Cu5/mZIF-8) was obtained via lowering the precursor ratio of ZIF-8 and incorporating copper ions during the synthesis process. Cu5/mZIF-8 were found to be nanospheres with an average diameter of around 150 nm. Laccase encapsulated in Cu5/mZIF-8 showed an activity recovery of 75.6%, which was 2.2 times higher than that of the laccase embedded in ZIF-8. Meanwhile, the immobilized laccase (Lac@Cu5/mZIF-8) showed a higher catalytic activity in organic solvents than that of the free enzyme. In the presence of a mediator, Lac@Cu5/mZIF-8 could remove 95.7% of reactive black 5 in 40 min. After four consecutive cycles, the dye decolorization efficiency declined to 28%. About four transformation products of reactive black 5 were identified via LC-MS analysis, and the potential decolorization mechanism was proposed. The results indicated that the immobilized laccase could be used as an efficient biocatalyst in dye decolorization.Keywords:laccase;zeolitic imidazolate framework-8;immobilization;dye decolorization
研究领域:
生物化学
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环境科学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2020
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IF 6.306
Shuo Shi,Wentao Zhang,Xinnan Liu,Xinyi Ren,Min Li,Jing Sun,Yutang Wang,Tianli Yue,Jianlong Wang
Lycium barbarum L. polysaccharides (LBPs) with outstanding biological activities are of increasing interest. Traditional purification approaches are time-consuming and often involve toxic solvents that destroy the functionality and structure of polysaccharides. Herein, we report a sustainable and nondestructive strategy for purifying LBPs using graphene-based nano-decoloration. The amination of graphene oxide (GO) enables the resulted aminated reduced GO (NH 2 -rGO) with abundant sp 2 -hybridized carbon domains, displaying high adsorption capacity toward pigments in crude polysaccharides. As such, within 5 min, NH 2 -rGO can highly effectively and fast to decolor LBPs, with a high decoloration ratio of 98.72% and a high polysaccharides retention ratio of 95.62%. Importantly, compared with traditional decoloration methods, NH 2 -rGO is nondestructive toward LBPs and has good reusability. Moreover, it exhibited widespread-use decoloration performance to decolor several common plant species. Overall, our proposed nano-decoloration approach is a general-purpose, sustainable, and nondestructive method to purify LBPs.
研究领域:
植物学
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生物化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2020
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IF 3.573
Haixia Xu,Xiaofeng Li,Xuan Xin,Kun Yuan,Hui Wu,Guanglei Zhao
Phenolic glycosides are the major secondary metabolites of plants, which exhibit multiple attractive biological and pharmacological activities. In this study, we developed a new and efficient method for acyl modification of phenolic glycosides by using whole-cell biocatalysts, aiming to improve their liposolubility and bioactivities. Using resveratrol, salidroside, piceid, and phlorizin as substrates, the substrate recognition characteristics of Candida parapsilosis were studied and salidroside showed the highest conversion (99.5%) but lower regioselectivity than others. Subsequently, the mechanism underlying the influences of the reaction time and organic solvents on the conversion and monoester ratio was revealed. A higher conversion of piceid was obtained in a green solvent (γ-valerolactone) than that in THF (95.8 vs 94.6%). Compared to their parent compounds, all monoester products exhibit higher liposolubility and antioxidant activity. In addition, the monoesters of salidroside and phlorizin showed significantly higher tyrosinase-inhibiting and depigmentation activities than their parents.
研究领域:
生物化学
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药理学
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植物化学
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DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
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2020
·Vol. 44
·期 4
·727-735
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IF 2.419
Wang Hongrao,Han Shuran,Wang Jiayi,Yu Shuyu,Li Xiaoyan,Lu Lei
Laccases are versatile oxidases that are capable of decolorizing various synthetic dyes. Recombinant Bacillus amyloliquefaciens laccase was immobilized as magnetic cross-linked enzyme aggregates (M-CLEAs) for application in dye decolorization. Several parameters influencing the activity recovery were evaluated during the synthesis of M-CLEAs. With ammonium sulfate as precipitant, maximum activity was recovered by cross-linking with 0.16% glutaraldehyde for 1 h. The prepared M-CLEAs exhibited improved activity under alkaline conditions. It remained 74% activity after incubation at 60 °C for 5 h. Enhanced tolerance towards NaCl was also observed for the M-CLEAs, with 68% activity remaining in the presence of 1 M NaCl. The immobilized laccase could rapidly decolorize more than 93% of reactive black 5 and indigo carmine in 1 h, while its catalytic efficiency towards reactive blue 19 was relatively low. After four cycles of consecutive reuse, the M-CLEAs could decolorize 92% of indigo carmine. The easy recovery and reusability of M-CLEAs facilitate the potential application of bacterial laccase in dye decolorization.
研究领域:
生物化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
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IF 4.784
Weichuan Qiao,Hai Liu
In this study, a CotA laccase from Bacillus subtilis cjp3 was successfully immobilized onto magnetic graphene oxide (MGO) nanomaterials via covalent bonding with hydrochloride/N-hydroxysuccinimide (EDC/NHS). The morphology, structure, and properties of the MGO-laccase were then characterized by scanning-electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), X-ray-photoelectron spectroscopy (XPS), and a magnetic-property-measurement system (MPMS). The magnetic composite exhibited an extremely high binding capacity of ~145.04 mg/g and maintained maximal relative enzyme activities at 25 °C, pH 7, and a reaction time of 2 h. The pH, thermal, operational, and storage stabilities of MGO-laccase were significantly improved over those of free laccase. Moreover, MGO-laccase exhibited a higher tolerance than that of free laccase in the presence of organic solvents , inhibitors, metal ions, and salts. Furthermore, MGO-laccase showed good decolorization performance of malachite green (MG), with decolorization rates reaching 99% after 5 h of reaction at 30 °C and pH 6. In addition, the maximum saturation magnetization of MGO-laccase was 27.7 emu/g, allowing for rapid magnetic separation. Accordingly, magnetic separation allowed MGO-laccase to maintain 75% of its activity after ten consecutive decolorization cycles.
研究领域:
生物技术
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环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Wenxing Jin,Jun Nan,Meng Chen,Langrun Song,Fangmin Wu
To achieve economical and efficient decolorization, two novel flocculants , weakly hydrophobic comb-like chitosan-graft-poly (N, N-Dimethylacrylamide) (CSPD) and strongly hydrophobic chain-like chitosan-graft- L -Cyclohexylglycine (CSLC) were synthesized in this study. To assess the effectiveness and application of CSPD and CSLC, the impacts of factors, including flocculant dosages, initial pH, initial dye concentrations, co-existing inorganic ions and turbidities , on the decolorization performance were explored. The results suggested that the optimum decolorizing efficiencies of the five anionic dyes ranged from 83.17% to 99.40%. Moreover, for accurately controlling flocculation performance, the responses to flocculant molecular structures and hydrophobicity in flocculation using CSPD and CSLC were studied. The Comb-like structure gives CSPD a wider dosage range for effective decolorization and better efficiencies with large molecule dyes under weak alkaline conditions. The strong hydrophobicity makes CSLC more effective in decolorization and more suitable for removing small molecule dyes under weak alkaline conditions. Meanwhile, the responses of removal efficiency and floc size to flocculant hydrophobicity are more sensitive. Mechanism studies revealed that charge neutralization, hydrogen bonding and hydrophobic association worked together in the decolorization of CSPD and CSLC. This study has provided meaningful guidance for developing flocculants in the treatment of diverse printing and dyeing wastewater.
研究领域:
环境科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
CHINESE CHEMICAL LETTERS
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2022
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IF 8.455
Han-Qing Zhao,Wen-Qiang Li,Nannan Hou,Lei Li,Yiran Wang,Peili Lu,Yang Mu
Pyrogenic carbonaceous matter (PCM) catalyzes azo dye decolorization by sulfide, but the nitrogen doping catalytic mechanisms are poorly understood. In this study, we found that stagnate time of azo dye methyl orange (MO) decolorization was reduced to 0.54-18.28 min in the presence of various nitrogen-doped graphenes (NGs), remarkably lower compared to graphene itself. Particularly, graphitic nitrogen played a critical role in NGs-catalyzed MO decolorization by sulfide. Gas chromatography-mass spectrometry and in-situ surface Raman analysis demonstrated that doping nitrogen, especially graphite one facilitated reactive intermediate polysulfides formation. This is attributed to the improved electron conductivity through graphitic nitrogen doping, and the enhanced interactions between sulfide and carbon atoms bonded to graphitic nitrogen. This study not only provides a better understanding of PCM impact on transformations and fates of organic pollutants in natural environments, but also offer a new regulation strategy for more efficient wastewater treatment processes in PCM-catalyzed engineering systems.
研究领域:
环境科学
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化学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2014
·Vol. 49
·期 12
·2571-2577
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IF 1.354
Chuqiao Xiao,Lin Zheng,Guowan Su,Mouming Zhao
Summary Activated carbon was used to remove the colour of peanut meal hydrolysate. Effects of solution pH and activated carbon dosages on the decolourization efficiency, nitrogen components and antioxidant activity of the hydrolysate were investigated. The results showed that the colour and the undigested protein or large peptides could be removed efficiently under acidic condition (pH 2.0–4.0), while less small peptides were adsorbed under this condition. Among these total amino acids (AAs), the loss of aromatic AA (Tyr and Phe) was the largest regardless of the solution pH. Greater loss of acidic AA (Glu and Asp) was observed under acidic condition, while the loss of basic AA (His, Lys and Arg) followed the opposite trend. The antioxidant activity of the hydrolysate was also reduced after decolourization, which was likely due to the loss of total phenolics and peptides containing aromatic and/or acidic AA.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
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IF 5.279
Fei Li,Honglu Zhao,Ruijian Shao,Xiaoyu Zhang,Hongbo Yu
The Fenton reaction is considered to be of great significance in the initial attack of lignocellulose in wood-decaying fungi. Quinone redox cycling is the main way to induce the Fenton reaction in fungi. We show that lytic polysaccharide monooxygenases (LPMOs), through LPMO-catalyzed oxidation of hydroquinone, can efficiently cooperate with glucose dehydrogenase (GDH) to achieve quinone redox cycling. The LPMO/GDH system can enhance Fe3+-reducing activity, H2O2 production, and hydroxyl radical generation, resulting in a fueled Fenton reaction. The system-generated hydroxyl radicals exhibited a strong capacity to decolorize different synthetic dyes and degrade lignin. Our results reveal a potentially critical connection between LPMOs and the Fenton reaction, suggesting that LPMOs could be involved in xenobiotic compound and lignin degradation in fungi. This new role of LPMOs may be exploited for application in biorefineries.
研究领域:
微生物学
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环境科学
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生物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Li-Shu Zhou,Yan-Shu Xiong,Ran Jia,Ming-Xing Li,Bo-Huan Fan,Jia-Yi Tang,Wen Li,Hai-Qin Lu,Yu-Wei Lan,Kai Li
The crucial need for quality refined sugar has led to the development of advanced adsorbents, with a focus on the decolorization of remelt syrup. In this study, (3-chloro-2-hydroxypropyl) trimethylammonium chloride and polyethyleneimine co-modified pomelo peel cellulose-derived aerogel (CP-PPA) was fabricated, and synthetic melanoidins were used as model colorants of remelt syrup to evaluate the validity and practicality of CP-PPA for eliminating colored impurities. Integrating abundant amine-functionalized groups (quaternary ammonium and protonated amine) within the pomelo peel-derived aerogel directionally captured electronegative melanoidins via electrostatic interactions. Furthermore, the active sites, types, and relative strength of the weak interactions between CP-PPA and melanoidins were determined using density functional theory simulations. CP-PPA exhibited an excellent equilibration adsorbing capacity for capturing melanoidins of 749.51 mg/g, and a removal efficiency of 93.69 %. Additionally, the adsorption mechanism was thoroughly examined in an effort to improve the economy of the sugar refinement industry.
研究领域:
食品科学
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材料科学
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DOI
PubMed
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2024
·Vol. 47
·期 3
·355-366
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IF 3.5
Duan Xiaoyan,Pi Qian,Tang Lei
Dyes in wastewater have adverse effects on the environment and human health. Dye-decolorizing peroxidase (DyP) is a promising biocatalyst to dyes degradation, but the decolorization rates varied greatly which influencing factors and mechanisms remain to be fully disclosed. To explore an effective decolorizing approach, we have studied a DyP from Rhodococcus jostii ( Rh DyPB) which was overexpressed in Escherichia coli to decolorize four kinds of dyes, Reactive blue 19, Eosin Y, Indigo carmine, and Malachite green. We found the decolorization rates of the dyes by purified Rh DyPB were all pH-dependent and the highest one was 94.4% of Malachite green at pH 6.0. ESI–MS analysis of intermediates in the decolorization process of Reactive blue 19 proved the degradation was due to peroxidase catalysis. Molecular docking predicated the interaction of Rh DyPB with dyes, and a radical transfer reaction. In addition, we performed decolorization of dyes with whole E. coli cell with and without expressing Rh DyPB. It was found that decolorization of dyes by E. coli cell was due to both cell absorption and degradation, and Rh DyPB expression improved the degradation rates towards Reactive blue 19, Indigo carmine and Malachite green. The effective decolorization of Malachite green and the successful application of whole DyP-overexpressed cells in dye decolorization is conducive to the bioremediation of dye-containing wastewaters by DyPs. Graphical abstract
研究领域:
环境生物技术
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酶催化
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生物修复
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蛋白质工程
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废水处理
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）