Advanced Healthcare Materials
·
2024
·
IF 10
Delong Xu,Shenghui Bi,Jiejing Li,Shaodan Ma,Ze-an Yu,Yenan Wang,Huiming Chen,Jie Zhan,Xudong Song,Yanbin Cai
Promoting angiogenesis and modulating the inflammatory microenvironment are promising strategies for treating acute myocardial infarction (MI). Macrophages are crucial in regulating inflammation and influencing angiogenesis through interactions with endothelial cells. However, current therapies lack a comprehensive assessment of pathological and physiological subtleties, resulting in limited myocardial recovery. In this study, legumain-guided ferulate-peptide nanofibers (LFPN) are developed to facilitate the interaction between macrophages and endothelial cells in the MI lesion and modulate their functions. LFPN exhibits enhanced ferulic acid (FA) aggregation and release, promoting angiogenesis and alleviating inflammation. The multifunctional role of LFPN is validated in cells and an MI mouse model, where it modulated macrophage polarization, attenuated inflammatory responses, and induces endothelial cell neovascularization compare to FA alone. LFPN supports the preservation of border zone cardiomyocytes by regulating inflammatory infiltration in the ischemic core, leading to significant functional recovery of the left ventricle. These findings suggest that synergistic therapy exploiting multicellular interaction and enzyme guidance may enhance the clinical translation potential of smart-responsive drug delivery systems to treat MI. This work emphasizes macrophage-endothelial cell partnerships as a novel paradigm to enhance cell interactions, control inflammation, and promote therapeutic angiogenesis.
研究领域:
心血管疾病
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纳米技术
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药理学
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细胞生物学
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治疗学
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DOI
PubMed
高影响因子
Biology-Basel
·
2025
·Vol. 14
·期 12
·1746
·
IF 3.5
Shuhui Fu,Fangyuan Li,Xizhong Yan,Chi Hao
Phytophagous insects rely on highly evolved chemosensory systems to recognize plant cues, which guide essential behaviors such as feeding and oviposition. While the antennal chemosensory functions of Plutella xylostella are well-studied, little is known about the role of its legs in detecting host plant chemicals. This study systematically identified and characterized chemosensory-related genes expressed in the legs of P. xylostella using transcriptome data. A total of 114 leg-expressed chemosensory genes were identified, with PxylCSP9 showing high expression in legs and strong, broad-spectrum binding to key cruciferous plant volatiles. Leg chemosensory proteins, particularly PxylCSP9, play an important role in the chemical perception. Plutella xylostella , a major pest of cruciferous vegetables, depends predominantly on chemoreception to locate host plants. Legs are crucial in insect chemical perception, particularly during close-range and contact chemoreception. However, the molecular basis underlying the chemosensory repertoire in P. xylostella legs remains elusive. To address this, we sequenced chemosensory-related genes in diamondback moth legs. Sequencing identified 32 odorant binding protein (OBP), 18 chemosensory protein (CSP), 26 odorant receptor (OR), 20 gustatory receptor (GR), 15 ionotropic receptor (IR), and 3 sensory neuron membrane protein (SNMP) genes. Comparative analysis with antennal transcriptome data revealed three CSPs, seven ORs, and two GRs newly identified in the legs. Transcriptome analysis showed higher fragments per kilobase of transcript per million mapped reads values for CSPs than for other chemosensory-related gene families. Furthermore, qRT-PCR confirmed the highest expression of PxylCSP9 in the legs, suggesting its role in perceiving external compounds. Fluorescent binding assays revealed high binding affinity of PxylCSP9 for several host plant semiochemicals. Molecular docking predicted a hydrophobic binding pocket in PxylCSP9 with Met11, Leu13, and Leu43 frequently participating in ligand interactions. Our findings indicate that leg-enriched PxylCSP9 is pivotal for host plant recognition during close-range chemoreception, suggesting its potential as a molecular target for precision management through behavior-based strategies.
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高影响因子
Comparative Biochemistry and Physiology D-Genomics & Proteomics
·
2025
·
IF 2.4
Sumbul Mureed Mastoi,Yang Hua,Waqar Ali Chandio,Paras Mureed Mastoi,Yang Wei
The female parasitoid wasp Scleroderma sichuanensis (Hymenoptera: Bethylidae) utilizes various intricate behavior strategies, including host detection, learning, and memory, to enhance its survival and reproductive success. RNA-Seq analysis conducted under sublethal insecticide stress identified three differentially expressed genes, SsOR5, SsGRIN2B, and SsPDE8A, selected for functional validation due to their likely roles in host searching behavior. The SsOR5, an odorant receptor, is believed to interact with other olfactory receptors and downstream signaling molecules to improve host detection. In contrast, SsGRIN2B, an NMDA-type glutamate receptor, may influence synaptic plasticity and neural transmission, thereby affecting leering behavior. Additionally, SsPDE8A modulates cAMP-dependent signaling pathways, which are crucial for memory consolidation and behavioral response. This research investigates these genes in S. sichuanensis using RNA interference (RNAi) to assess changes in host finding efficiency. Adult female wasp received dsRNA treatments at doses of (0.5 μL, 1 μL, and 3 μL) and time durations (12, 24, 48, and 72 h), with the most significant gene silencing observed at 3 μL for 48–72 h. Behavioral assays conducted with a Y-tube olfactometer revealed that knockdown of SsOR5 significantly reduced host attraction, while knockdown of SsGRIN2B and SsPDE8A led to moderate reeducations, highlighting the varying contributions of these genes to olfactory drive behavior. This study demonstrates that insecticides induced changes in gene expression can disrupt neural and olfactory pathways essential for host location, and emphasizes the potential of RNAi-based approaches to target behaviorally significant genes, offering new strategies for parasitoid-mediated pest management.
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高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2022
·
IF 4.52
Shiqi Xu,Miao Liang,Binshan Liu,Zhimin Zhang,Yuping Liu,Zhengwei Liu,Yudong Wang
To identify the key odorants in braised chicken thigh meat (BCTM) and to investigate their changes during BCTM storage from 1 month to 23 months at room temperature , volatiles were isolated by both solid-phase microextraction and solvent extraction combined with solvent-assisted flavor evaporation; a total of 27 odor-active regions were detected in the isolates by gas chromatography–mass spectrometry–olfactometry. Twenty-four odor-active compounds were identified positively by comparison of their odor characteristics, mass spectral data and retention indices with those of authentic compounds; their flavor dilution (FD) factors ranging from 1 to 512 were measured by aroma extract dilution assay. Eugenol had the highest FD factor of 512. The odorants with FD factors ≥ 4 were quantitated by internal standard curve method, and their odor activity values (OAVs) were calculated. 2-Methyl-3-furanthiol and (E,E) -2,4-decadienal had the highest OAVs. Fourteen compounds with OAVs ≥ 1 were determined to be the key odorants of BCTM; recombination experiments confirmed that these odorants could simulate the overall aroma profile of BCTM. To further investigate the changes in odorants during storage, their concentrations and OAVs were determined. The results showed that the number of key odorants did not change, and no off-odor compound was detected.
研究领域:
食品科学
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香气化学
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食品储存
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高影响因子
Bioactive Materials
·
2020
·
IF 8.724
Fuxin Jiang,Jian Ren,Yachai Gao,Jinna Wang,Yiping Zhao,Fengying Dai
Brain barrier is both a protective permeability hurdle and a limitation site where therapeutic agents are excluded to enter the target region. Designing drug vehicle to overcome this notorious barrier bottle is challenging. Herein, we construct a stimuli-responsive self-assembled nanovesicle that delivers water-soluble drugs to prevent the efflux transport of brain barriers by responding to the endogenously occurring signals in Alzheimer's disease (AD) brain microenvironment. Once stimuli-responsive vesicles are accumulated in intracerebrally, the intrinsically occurring legumain endopeptidase cleaves the Ac-Ala-Ala-Asn-Cys-Asp (AK) short peptide on the drug vesicles to expose the 1,2 thiol amino group to cyclize with the cyano groups on 2-cyano-6-aminobenzothiazole (CABT) of the chaperone vesicles, thus triggering the formation of cross-linked micrometre-scale vesicles. Such a structural alteration completely prevents further brain barriers efflux. The superior neuroprotective capacity of cross-linked vesicles is validated in senescence accelerated mouse prone 8 (SAMP8). This smart multi-drug delivery vesicle is promising to serve as a powerful system for AD treatment and can be adapted for the therapy of other central nervous system (CNS) disorders.
研究领域:
神经科学
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药剂学
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分子生物学
·
药物递送系统
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PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2024
·
IF 2.9
Li Huanhuan,Zhao Ke,Chen Lihong,Huang Qicheng,Ma Xiaozhong,Ge Shengyuan,Su Mingwei,Tang Honggang
In the present study, we evaluated the physicochemical properties, free amino acids concentration, metabolomics, and the relationship between main metabolites and volatile compounds of Jinhua ham prepared using Taihu black pigs during a ripening period of 210 days to elucidate the distinguishing features and formation process of the quality of this ham. During the eight timepoints, the color parameters redness and yellowness exhibited significant changes. Water activity gradually decreased. The tastes of saltiness, sourness and umami were main taste characteristics of Jinhua ham. Carnosine and glutamic acid were the main umami taste compounds and their contents reached to 1785 ± 92.2 and 699 ± 65.1 mg/100 g respectively. Metabolomics-based analysis showed that the dominant biochemical reaction was the metabolism of carboxylic acids and their derivatives. Total 153 significantly differential metabolites were identified. The main metabolic pathways were different in the three different periods respectively. Lipid metabolism was the predominant pathway in low–temperature stage, while amino acid formation and protein metabolism were the predominant pathways in the moderate–temperature and high–temperature stage. The dominant flavor compounds were closely related to the key differential metabolites. Our study findings revealed the quality, significantly differential metabolites, and formation process of Jinhua ham prepared using Taihu black pigs.
研究领域:
食品科学
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代谢组学
·
农业
·
生物化学
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DOI
高影响因子
ACS Applied Polymer Materials
·
2024
·
IF 4.4
Yunfei Liu,Yayu Feng,Yue Sun,Meijing Zhang,Pengfei Zhang,Ronghui Zhao,Kuilin Deng
In this investigation, a nonconjugated fluorescent polyamide with piperazine and valerolactam moieties (PAM–PV) has been synthesized by the modified Ugi polymerization using 1,4-bis(3-aminopropyl)piperazine, levulinic acid, and 1,4-bis(3-isocyanopropyl)piperazine as monomers. In the polymerization, the special construction of levulinic acid leads to the formation of valerolactam on PAM–PV chains via the Mumm rearrangement. PAM–PV shows a strong fluorescence emission at 428 nm due to the intramolecular aggregation of piperazine and valerolactam moieties along the backbone. Impressively, PAM–PV can specifically detect NO2– ions via static quenching with a linear range of 10–6–10–5 M and a detection limit of 0.6 μM (0.0276 mg/L), which was far less than the minimum allowable concentration of 3 mg/L for NO2– in drinking water specified by WHO. Furthermore, the several common anions and cations have no interference effects on the specific detection of PAM–PV to NO2– ions. The quenching mechanism of NO2– and PAM–PV by FTIR, UV, and 1H NMR measurements indicates that the strong electrostatic interaction between NO2– and PAM–PV makes the macromolecular chains more relaxed and outstretched, leading to a formation of a nonfluorescent complex. Moreover, the practical detection of NO2– in the three water bodies, sauerkraut, and ham sausage and the fabrication of a portable detecting paper to NO2– ions were conducted in this experiment.
研究领域:
聚合物化学
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分析化学
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环境科学
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DOI
高影响因子
Frontiers in Cellular and Infection Microbiology
·
2021
·
IF 5.293
Li Xiaobo,Song Yanqing,Wang Lina,Kang Guangbo,Wang Ping,Yin Huabing,Huang He
Multidrug-resistant (MDR) Acinetobacter baumannii strains can cause severe infections in intensive care units, and are rapidly developing resistance to the last-resort of existing antibiotics, posing a major global threat to health care system. Berberine hydrochloride (BBH), a kind of isoquinoline alkaloids extracted from Berberis and other plants, has been widely used as an antibacterial medicine for its reliable therapeutic efficiency. The in vitro synergistic effects of BBH with antibiotics against MDR A. baumannii were determined. BBH alone had weak antimicrobial activity (e.g., MIC≥256 mg/L) against MDR A. baumannii. However, it dramatically increased the susceptibility of MDR strains against antibiotics with FICI values <0.5, even reversed their resistance to antibiotics (e.g., tigecycline, sulbactam, meropenem and ciprofloxacin). In vivo study has suggested BBH with sulbactam had stronger antimicrobial efficiency than monotherapy in a neutropenic murine thigh infection model. The antibiotic-sensitizing mechanism of action of BBH was evaluated as well. BBH boosted adeB gene expression and bound to the AdeB transporter protein, resulting in low uptake of BBH, which may contribute to less extrusion of antibiotics by the AdeABC pump. Knockout of the adeB gene increased uptake of BBH and diminished the antibiotic sensitization and synergistic effects between antibiotics and BBH in MDR strains. Together, BBH effectively re-sensitizes this MDR pathogen to a range of antibiotics that have become barely effective due to antibiotic resistance, which indicates BBH may be a promising therapeutic adjuvant candidate to combat MDR A. baumannii.
研究领域:
微生物学
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药理学
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传染病学
·
分子生物学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Ling Qiu,Xi Li,Gaochao Lv,Yann Seimbille,Ke Li,Ying Peng,Qingzhu Liu,Minhao Xie,Jianguo Lin
Overexpression of legumain is closely associated with tumor proliferation, invasion, and metastasis. Because of its intrinsic properties, such as high sensitivity and resolution, positron emission tomography (PET) has become an effective imaging technique for early diagnosis, treatment response prediction, and monitoring. Herein, two legumain-targeting radiofluorinated smart probes (18F-2 and 18F-3) as well as a control probe (18F-1) were specifically designed for PET imaging of legumain activity in tumors. 18F-1, 18F-2, and 18F-3 were obtained with high radiochemical yield (RCY > 60%) and radiochemical purity (RCP > 99%) using a convenient “one-step” 18F-labeling method. The probes 18F-2 and 18F-3 exhibited high response to legumain activity and reductive environment and revealed comparable uptake in HCT116 cells (4.22% ± 0.14% and 4.64% ± 0.32% for 18F-2 and 18F-3, respectively; 8.46% ± 0.33% and 9.05% ± 0.24% for co-treatment of 18F-2 + 2 and 18F-3 + 3 at 1 h), while the control probe 18F-1 showed no response. PET imaging of tumor-bearing mice showed that the co-injection strategy (18F-2 + 2 and 18F-3 + 3) resulted in higher tumor uptake (3.57% ± 0.37% and 3.72% ± 0.19% ID/g at 10 min, respectively) than the single injection strategy (2.59% ± 0.19% and 2.60% ± 0.46% ID/g for 18F-2 and 18F-3, respectively). In addition, introduction of the trimeric histidine-glutamate (HEHEHE) tag to 18F-3 reduced the liver uptake by almost two-fold without any noticeable effect on the tumor uptake. All the results indicate that 18F-3 holds great potential applications in clinics for sensitive and specific PET imaging of legumain activity in tumors.
研究领域:
医学成像
·
癌症研究
·
分子生物学
·
放射学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 8
·1-9
·
IF 5.7
Yang Chunlei,Xu Guiju,Hou Chenghao,Peng Lizeng,Wang Weiting,Zhang Hongwei,Zhang Xiaoling
A fluorometric and colorimetric dual-modal nanoprobe (denoted as Fe 2+ -Phen/SiNPs) has been developed for selective and sensitive determination of nitrite (NO 2 − ). The mechanism is based on fluorescence quenching between silicon nanoparticles (SiNPs) and Fe(II)-phenanthroline complex (Fe 2+ -Phen) via inner filter effect and redox. With the addition of increasing NO 2 − , Fe 2+ is oxidized to Fe 3+ , recovering the fluorescence of SiNPs. Meanwhile, the color of the system gradually changes from orange-red to colorless, which enables colorimetric measurement. The NO 2 − concentration shows a wide linear relationship with fluorescence intensity from 0.1 to 1.0 mM ( R 2 = 0.9955) with a detection limit of 2.4 μM in the fluorometric method (excitation wavelength: 380 nm). By contrast, the linear range of the colorimetric method ranges from 0.01 to 0.35 mM ( R 2 = 0.9953) with a limit of detection of 6.8 μM (proposed selective absorbance: 510 nm). The probe has been successfully applied to nitrite determination in water, salted vegetables, and hams demonstrating broad application prospects for the determination of nitrite in complicated matrices. Graphical Abstract
研究领域:
分析化学
·
纳米技术
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Lang Zhang,Xiufang Xia,Fangda Sun,Haotian Liu,Qian Chen,Baohua Kong
This study aims to investigate the inhibitory effects of hydrocolloids on heterocyclic aromatic amine (HAA) formation in chemical model systems and smoked chicken drumsticks. In chemical model systems I and II, xanthan gum (XG) and carboxymethyl cellulose sodium (CMC-Na) effectively inhibited the 2-amino-1-methyl-6-phenylimidazo[4,5- b ]pyridine (PhIP), 1-methyl-9H-pyrido[3,4- b ]indole (Harman), and 9H-pyrido[3,4- b ]indole (Norharman) formation in comparison with the control sample ( P < 0.05). Arabic gum (AG) inhibited the PhIP and Harman formation ( P < 0.05), but its inhibitory action against Norharman formation was not significant ( P > 0.05). Further mechanism investigation of chemical model system I showed that the formation of PhIP was mainly inhibited via trapping phenylalanine and creatinine by glyoxal and methyglyoxal. A mechanistic investigation of chemical model system II suggested the inhibition of Harman and Norharman formation by XG and CMC-Na could be through trapping tryptophan by glyoxal and methyglyoxal. In smoked chicken drumsticks, CMC-Na had a dose-dependent inhibitory effect against the PhIP, Harman, and Norharman formation in comparison with the control sample ( P < 0.05), and 2.0% CMC-Na had the highest inhibitory rates of 45.83%, 21.82%, and 30.92%, respectively. Moreover, CMC-Na effectively inhibited the formation of PhIP, Harman, and Norharman precursors and intermediates in smoked chicken drumsticks, which further confirmed the above inhibition mechanism of CMC-Na. These results could provide an effective approach to reduce HAA formation in smoked meat products by incorporating hydrocolloids.
研究领域:
食品科学
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化学
·
毒理学
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高影响因子
ENERGY CONVERSION AND MANAGEMENT
·
2023
·
IF 10.4
Pengcheng Zhu,Xuepeng Luo,Xinran Lin,Zhichong Qiu,Rongrui Chen,Xiangcheng Wang,Yaling Wang,Yuan Deng,Yanchao Mao
Flexible thermoelectric devices (f-TEDs) can realize direct energy conversion between heat and electricity, which hold great prospects in wearable power source and personal thermal management. However, conventional f-TEDs made from intrinsically flexible thermoelectric materials have low power density, and elastomer sealed bulk thermoelectric materials-based f-TEDs can hardly achieve active cooling. Additionally, these f-TEDs usually are not self-healable and recyclable, which easily occur fractures in wearable applications. Here, we developed a self-healable and recyclable f-TED by integrating dynamic covalent thermoset polyimine with liquid metal and thermoelectric legs. This f-TED achieves a normalized power density of 1.54 μW·cm −2 ·K −2 , and can deliver a record 13.8 °C on-body cooling effect with a high coefficient of performance (COP) at 3.91 under 7 °C temperature difference, which leads to a low power consumption of ∼ 38 W for the cooling of regular human body. Based on the f-TED, we further developed a personal thermal management system, which can keep body within comfort zone at different surrounding temperatures, also realize healthcare function for fever or sprained ankle. Compared with conventional thermoelectric devices, this f-TED can simultaneously achieve self-healability, recyclability, flexibility, large normalized power density, and high on-body cooling effect with low power consumption. Such f-TED could open up new opportunities to develop devices for wearable energy harvesting and personal thermal management.
研究领域:
材料科学
·
热电学
·
可穿戴技术
·
能量收集
·
个人热管理
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DOI
高影响因子
危险品化学品经营许可证（带存储）