JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2024
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IF 3.3
Qihong Zhang,Xinyu Lin,Weike Su
BACKGROUND In this study, the content and structure of Polygonatum sibiricum polysaccharides and saponins during different processing stages were determined. RESULTS After processing of Polygonatum , the content of polysaccharide and glucose decreased, and the content of galactose, glucuronic acid and sugar substitution gradually increased. The content of total saponins increased significantly. Only 18 compounds were found in raw Polygonatum and 17 new compounds were presented in processed Polygonatum . During the processing of Polygonatum , the polysaccharide was partially degraded into oligosaccharides, the molecular weight gradually decreased, and the neutral sugar was converted into uronic acid, resulting in a decrease in polysaccharide content. The saponins were partially degraded into sapogenins or modified. CONCLUSION This study clarifies the changes in the content and structure of polysaccharides and saponins in processed Polygonatum , which will pave the way for elucidating the processing mechanism. © 2024 Society of Chemical Industry.
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Food Bioscience
·
2024
·
IF 4.8
Qingzhi Ding,Yuan Zheng,Yongqi Zhu,Huamin Yang,Lin Luo,Haile Ma,Xiaoran Li
The individual and combined inhibitory activities of propolis extract (PE) and Hermetia illucens larval proteins (Black soldier fly larvae proteins) (BSFLP) against Aspergillus niger were investigated. The antifungal effects of PE, BSFLP and Synergy of PE and BSFLP (SPB) against Aspergillus niger were determined by mycelial growth , spore germination rate and hyphal elongation. Membrane integrity was measured by assessing changes in extracellular proteins, reducing sugars, and electrical conductivity . Intracellular MDA , H 2 O 2 , ergosterol , and mannan contents were also evaluated. SEM was used to observe the morphology of the Aspergillus niger mycelium . Additionally, AutoDock Tools software and multi-ligand docking methods were used to study the synergistic interactions of ligand combinations on the receptor. The results showed that the combination of PE and BSFLP had excellent effects. During the treatment of antifungal agents, the membrane permeability changed and a large amount of content leaked, while control check (CK) remained almost unchanged. Ergosterol content was found to increase, while mannan content decreased significantly, leading to cell death. In addition, SPB can cause abnormal accumulation of MDA and H 2 O 2 , leading to oxidative stress in cells. SEM results showed that morphology of Aspergillus niger treated with SPB was severe wrinkling, distortion and even breakage. Multi-ligand docking results showed that the binding affinity of receptor 1KS5 to 14 complex ligands increased, as indicated by the decrease in docking energy values. These results provide scientific basis for further study on the application of SPB and development of effective antifungal products.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Yongfang Sun,Jinmei Xie,Canzheng Wang,Shaoxue Qin,Kun Ren,Dongmei Wang,Yingni Pan,Shumeng Ren,Xiaoqiu Liu
Curcumae wenyujin Rhizoma (CWR) is extensively implemented in clinical practice for treating primary dysmenorrhea (PD) resulting from qi stagnation and blood stasis. However, the development of CWR-derived polysaccharides remains poorly investigated. In this study, the polysaccharide CWP-3 was isolated from CWR, and structurally characterized through FT-IR, HPLC, GC–MS, and NMR, which identified it as an RG-I-type pectin polysaccharide with a molecular weight of 423.6 kDa. CWP-3 consists of the monosaccharides Rha, GalA, Gal, and Ara (25.7: 28.6:42.8:2.9). The main backbone consisted of alternating 1,2-linked Rha p and 1,4-linked GalA p residues, with Gal and Ara forming the side chains, respectively. SEM revealed a porous, sheet-like morphology, while Congo Red staining and AFM analyses confirmed a triple helix structure with a height of 3.37 nm, and TG-DSC analysis indicated good thermal stability. In a mouse model of PD, 160 mg/kg CWP-3 reduced writhing by 70.3 %, prolonged coagulation time by 37.5 %, restored uterine and hepatic indices, and decreased AST, ALT, D2D, TXB2, IL-6, IL-1β, and TNF-α, while normalizing PGF₂α/PGE₂ levels. These results indicate that CWP-3 exerts blood-activating and analgesic effects through cytokine-mediated COX-2 suppression, suggesting its potential as a therapeutic candidate for PD with qi stagnation and blood stasis.
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Bioresources and Bioprocessing
·
2025
·Vol. 12
·期 1
·1-17
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IF 5.1
Cai Wen,Zhang Qian-ying,Zhu Peng-cheng,Hu Wanrong,An Hongyue,Huo Dan-Qun,Li Dongliang
This study focuses on the aging process of strong-flavor flue-cured tobacco leaves (SCTL) and light-flavor flue-cured tobacco leaves (FCTL), and explores the effects of microbial metabolism and enzyme action on tobacco leaf components and quality during this process. It employs methods including high-throughput sequencing, functional prediction of microbial communities via the PICRUSt2/MetaCyc database, dynamic analysis of conventional components and enzyme activities, and redundancy analysis. The results showed that under the same aging duration, the microbial community diversity structures of tobacco leaves with different quality grades were similar, and a total of 21 species of differential bacteria and 9 species of differential fungi were screened out; During the aging process, the contents of starch and total phenols in tobacco leaves decreased significantly, reducing sugars and total sugars showed a fluctuating trend, while total nitrogen and total plant alkaloids had no significant changes. Among them, the starch content in SCTL was higher than that in FCTL, while the contents of total phenols, reducing sugars and total sugars in SCTL were lower than those in FCTL; the contents of total nitrogen and total plant alkaloids were comparable between the two types of tobacco leaves. Additionally, the contents of reducing sugars, total sugars and starch in both types of tobacco leaves were positively correlated with tobacco leaf grade, while the contents of total phenols and total nitrogen were negatively correlated with tobacco leaf grade; The enzyme activities in the two types of tobacco leaves showed fluctuating changes during aging, and redundancy analysis (RDA) identified significant correlations between microbial taxa, enzyme activities and tobacco leaf compounds. This study provides a basis for microbial strain selection and precision fermentation strategies in tobacco aging. Graphical abstract
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2025
·Vol. 26
·期 10
·4496
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IF 4.9
Chunyuhang Xu,Siyu Chen,Tiange Liu,Haowen Zhu,Chien-Liang Kuo,Zhuoyu Zhou,Guo Chen,Fion Wei Lin Chin,Xin Yang,Dejian Huang
Antrodia cinnamomea is widely known for its bioactive properties, particularly in anti-cancer, anti-inflammatory, and antibacterial areas. Despite the full use of the bioactive compounds from its fruiting body, high-value residues remain largely underexploited. This study presents a novel one-pot gel formation method, utilizing cinnamomea cellulose-riched residues to create hydrogels as an effective wound-healing dressing. The hydrogels derived from these residues show desirable properties, including non-drying characteristics, antibacterial activity against Staphylococcus aureus ATCC 1768, and cytocompatibility. Residual bioactive compounds, such as Antcin-K, Dehydroeburicoic acid, and (25S,R)-Antcin H, were identified in the residues, adding to the hydrogel’s efficacy. A UVB irradiation model was employed to evaluate the protective effects of the residues on UVB-damaged HaCaT skin cell lines, with an IC 50 of 0.045 mg/mL. The results indicated that A. cinnamomea residue extracts reduced the upregulation of MMP-1, MMP-2, MMP-3, MMP-7, and MMP-9 proteins caused by UVB exposure, suggesting high UV-protective activity. Additionally, antibacterial tests on Staphylococcus aureus strains, including Staphylococcus ATTC 1768, showed promising results, with inhibition zones ranging from 10.64 to 12.11 mm. In summary, Antrodia cinnamomea residue hydrogels combine UV protection with antimicrobial activity, making them a promising candidate for medical applications, particularly as a wound-healing dressing.
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ACS Applied Materials & Interfaces
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2025
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IF 8.2
Jiaqi Wu,Tianchong Li,Bowen Yu,Xuhui Sun,Lei Yang,Baosheng Du,Caixuan Yang,Jian Li,Shouxin Liu,Zhijun Chen,Shujun Li,Chenhui Yang
Metabolic regulation─the dynamic biochemical network governing energy transduction, substrate conversion, and metabolic flux─represents a fundamental determinant of microbial viability and functional output. While temporal metabolite fluctuations provide critical insights into metabolic network dynamics, conventional analytical platforms face fundamental limitations in real-time monitoring within native microbial environments. This study presents a novel whole-cell electrochemical biosensing platform integrating carbon dot (CD)-engineered Escherichia coli (E. coli) with advanced cyclic voltammetry (CV) for dynamic metabolic interrogation. This biohybrid system synergizes microbial biochemical specificity with CD-enhanced electron transfer efficiency, achieving nearly a 20-fold amplification in the electrochemical signal amplitude through quantum-enhanced charge transport mechanisms. The platform enables precise quantification of redox-active metabolites via distinct voltammetric fingerprints, as demonstrated through the detection of lactic acid─a pivotal biomarker in industrial biotechnology and clinical diagnostics. Featuring a modular bioarchitectural design, this technology permits seamless adaptation across diverse microbial systems, offering unprecedented capabilities for real-time bioprocess optimization, dynamic metabolic pathway analysis, and pathogen metabolic profiling. By interfacing nanomaterial-enhanced electrochemistry with synthetic biology, our platform surmounts traditional analytical constraints, establishing a versatile analytical tool for spatiotemporal mapping of metabolic networks in complex biological matrices.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Su Xu,Dajuan Shi,Haijiang Chen,Fengwei Ma,Guangcan Tao,Wenneng Wu,Dong Lin,Siyao Wu,Qiang Fei,Yan Hu,Lingshuai Meng
This study explored the influence of substrate cultivation system on the quality of ‘Hongyan’ strawberry through physicochemical and sensory evaluation , E -nose, GC–MS, and GC-IMS. Results revealed that substrate cultivation system significantly enhanced the formation of total acids, vitamin C , total phenols, and volatile esters, alcohols, and acids in strawberries. Additionally, it improved the hardness, acidity, aroma intensity, and color of the fruit. ROAV analysis of VOCs demonstrated that substrate cultivation system promoted green, floral, citrus, and wood aromatic notes, while introducing unique caramel and mint flavors. Through VIP values, three key physicochemical parameters and several vital volatile esters, alcohols, and aldehydes were identified as essential biomarkers for distinguishing strawberries grown under substrate cultivation system versus soil cultivation system. Correlation analysis underscored significant relationships between VOCs , particularly esters, aldehydes, and alcohols, and a notable positive correlation between ambient temperature, substrate temperature, fertilization frequency, and the physicochemical properties and characteristic VOCs.
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CARBOHYDRATE POLYMERS
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2013
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IF 3.479
Chao Zhong,Chunming Wang,Fan Huang,Honghua Jia,Ping Wei
In this article, a novel and high efficient solvent, tetra - n -Butylammonium Hydroxide (TBAH), was used for dissolution and isolation of straw cellulose from wheat straw. The composition analysis with gas chromatography (GC) and the spectroscopic characterization analysis conducted by X-Ray diffraction (XRD)/Fourier transform infrared spectroscopy (FTIR) indicated that straw cellulose can be directly dissolved and isolated by TBAH without derivatization . The investigation on the properties of straw cellulose isolation was based on the results of single factor experiments and orthogonal experiments, with optimum conditions for straw cellulose isolation being obtained as follow: reaction temperature 60 °C, reaction time 40 min, concentration of TBAH 50% and ratio of TBAH to straw 10:1 (m/m). In addition, as a solvent for cellulose, TBAH could be recycled several times with high activity being retained.
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APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Xiaoyan Li,Xinyu Zhang,Jialong Yi,Chenhu Mei,Jixiang Zhu,Ming Li,Longqi Zhao,Deng-guang Yu
Polymethyl methacrylate (PMMA) bone cement is widely used in orthopedic surgery, and enhancing its antibacterial properties is crucial for preventing perioperative infections. In this study, unique “berry-shaped” micro/nano composite particles were prepared via coaxial electrospraying for the development of antibacterial PMMA bone cement. These particles consist of PMMA microspheres uniformly embedded with nanosilica (SiO 2 ) drug carriers coated with quaternary ammonium chitosan (HACC) and loaded with gentamicin sulfate (GS). Using a polylactic acid (PLA) nanofiber mat as the collection substrate, uniform assembly of the micro/nano composite particles and their strong anchoring to the matrix were achieved by tailoring the core-sheath solution properties and leveraging capillary-driven interactions. This hierarchical design not only improved interfacial adhesion to the PMMA bone cement matrix and improved flexural toughness, but also reduced the curing temperature. The corresponding antibacterial PMMA composite bone cement released GS sustainably for over five days, demonstrating potent antibacterial activity against both Escherichia coli ( E. coli ) and methicillin-resistant Staphylococcus aureus ( MRSA ), as well as good fibroblast biocompatibility. This study proposed an integrated “material-structure-assembly” strategy for the one-step fabrication of functional micro/nano composite particles, providing a new approach for the development of advanced biomedical materials.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Daoliang Wang,Zhibin Liu,Weiying Su,Yuan Lu,Xiaoxiao Feng,Yuan Liu,Jinsu Lin,Bo Wen,Meijuan Xiao,Li Ni
Summer tea leaves, characterized by thick cell walls, low aroma, and strong bitterness, are generally considered unsuitable for high-quality tea production. This study investigated the potential of steam explosion technology (SET), which exposes the leaves to high-pressure steam followed by rapid depressurization, for processing these leaves. Under optimal condition (0.6 MPa, 180 s), SET led to significant cell wall disruption, enhancing the release of polyphenols. Sensory analysis showed that steam-exploded tea achieved a flavor profile comparable to white tea made from spring leaves. GC–MS analysis revealed markedly increased extraction of desirable volatile compounds. UPLC-QTOF-MS confirmed that reductions in flavanols, phenolic acids, and caffeine (by up to 15.61 % ∼ 35.28 %) contributed directly to reduced bitterness. Furthermore, steam-exploded tea was suitable for cold-water brewing, achieving high extraction rates of key compounds compared to hot-water brewed tea. These findings highlight SET as a sustainable solution for enhancing summer tea quality and usability.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Lulu Zhao,Yongbo Peng,Jing Huang,Nishang Liu,Xinrong Zou,Junnan Li,Yunpeng Fan,Ping Li,Liling Tang,Jisheng Wang,Yajun Zeng,Yi Wu,Gaohui Zhu
Polysaccharides are the major bioactive composition of Polygonatum sibiricum ( P. sibiricum ). However, the structural and functional identifications of these polysaccharides were still limited. Herein, we isolated a novel P. sibiricum polysaccharides (PSPF) and explored its potential function and mechanism in alleviating hyperlipidemia. PSPF were purified by diethylaminoethyl-sepharose fast flow (DEAE-Sepharose FF) and cross-linked dextran gel LH-20 (Sephadex LH-20) column chromatography, and identified by gel-permeation chromatography, methylation analysis, fourier transform infrared spectrometer (FT-IR), nuclear magnetic resonance (NMR), and gas chromatography–mass spectrometry (GC–MS). Their molecular weight (Mw), polysaccharide composition, and chemical structure were characterized. Furthermore, egg yolk emulsion-induced acute hyperlipidemia mouse model was constructed to evaluate the lipid-lowering efficacy and the underlying mechanism of PSPF. It was found that PSPF, with the Mw of 3592 Da, were prepared and mainly consisted of fructan with →1)-β-D-Fru f -(2 → main chain and →6)-β-D-Fru f -(2 → side chains. In addition, PSPF supplements efficiently reduced liver lipid accumulation, alleviated hepatocyte steatosis, and upregulated the AMP-activated protein kinase (AMPK) pathway, thereby enhancing fatty acid oxidation and decomposition. These results indicate that PSPF may serve as the potential dietary supplements for lipid reduction.
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危险品化学品经营许可证（带存储）