BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Zhe Lu,Jingcheng Dai,LingLing Zheng,Zhuoran Teng,Qi Zhang,Dongru Qiu,Lirong Song
Improving carbon availability in astaxanthin production is pivotal in Haematococcus industry. In this study, disodium 2-oxoglutarate (2-OG-2Na) was observed to be a potential carbon regulator to increase the astaxanthin content. To illustrate its efficacy in astaxanthin production, key genes and enzyme were analyzed. Upon 2-OG-2Na treatment, genes ipi , bkt and crtR-b were up regulated, concomitantly, carotenoids and astaxanthin content increased by 15.4% and 14.0% at 120 h, respectively; additionally, Acetyl-CoA carboxylase was activated, consistent with 1.27-fold increase in fatty acids content. PUFAs increased earlier as fatty acids assembly gene fad was up-regulated to 20.56. It was also found that cell division was not compromised. Altogether, it was suggested that increased carbon skeletons were re-directed into the astaxanthin and fatty acids biosynthesis pathway. Furthermore, 2-OG-2Na was applied in ten Haematococcus strains. Of these strains, astaxanthin contents were accelerated with average net increase of 10.48%, exhibiting a scalable paradigm for commercial production.
研究领域:
生物化学
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植物学
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工业微生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Yujun Ge,Yuan Li,Tiantian Wu,Yan Bai,Chunhong Yuan,Shiguo Chen,Ishimura Gakushi,Yaqin Hu
Effects of Chlorogenic acid-Gelatin (CGA-Gel) combined with partial freezing on quality change of sword prawn ( Parapenaeopsis hardwickii ) stored at −5 °C were evaluated for 23 days. Changes in sensory score, total viable counts (TVC), and physiochemical indexes including pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid reactive substances (TBARS) and Ca 2+ -ATPase were examined. All shrimp treated with CGA and CGA-Gel had lower total viable counts compared to control (P < 0.05). The value of TVB-N and TBA of CGA-Gel treated group at day 13 were 18.4 mg N/100 g and 0.175 mg/100 g respectively, both below the proposed safe limits and values of CGA treated group. All the results demonstrated that Chlorogenic acid can inhibit growth of microorganism, lipid oxidation and protein degradation. CGA-Gel treated samples presented better quality preservation effects than CGA treated alone. Therefore, CGA-Gel combined with partial freezing is promising in sword prawn shelf life extension.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2023
·
IF 6.1
Qiqi Dong,Weiqiang Qiu,Li Li,Ningping Tao,Ashley Liang Wang,Shanggui Deng,Yinzhe Jin
Binary ionic liquid mixtures (IL) were used for the first time to extract chitin from shrimp shells. Two ionic liquids, 1-ethyl-3-methylimidazole acetate ([EMIM][Ac]) and 1-butyl-3-methylimidazole bromide salt ([BMIM][Br]), were mixed at ratios of 0:1, 1:1, 3:2, 4:1, and 1:0 and the properties of the IL and the characteristics of extracted chitin were investigated. With an increase in proportion of [EMIM][Ac], the viscosity, dielectric constant, and dielectric loss of IL gradually decreased ( p < 0.05), whereas the conductivity, radius of gyration increased ( p < 0.05). The best quality of chitin using IL (3:2) was achieved when the viscosity, conductivity, and radius of gyration were 0.16 ± 0.00 Pa‧s, 0.30 ± 0.01 S/m, and 0.10 ± 0.00 nm, respectively. The yield of chitin extraction using IL was 35.72 ± 0.31 %, and this was approximately 2.04 times higher than that using the acid-base method (17.50 ± 0.16 %). The Whiteness index (WI) of chitin extracted using IL (3:2) (94.93 ± 0.09) was better than commercial chitin (93.31 ± 0.11), and the secondary structure, surface morphology and thermal stability were similar to commercial chitin. Therefore, the binary ionic liquid mixture can be used as an effective green solvent to extract chitin from shrimp shells.
研究领域:
生物化学
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材料科学
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环境科学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Xiang Wang,Jin-Hua Mou,Zi-Hao Qin,Ting-Bin Hao,Lan Zheng,Joseph Buhagiar,Yu-Hong Liu,Srinivasan Balamurugan,Yuhe He,Carol Sze Ki Lin,Wei-Dong Yang,Hong-Ye Li
The unicellular freshwater green alga Haematococcus pluvialis has attracted much research attention due to its biosynthetic ability for large amounts of astaxanthin, a blood-red ketocarotenoid that is used in cosmetics, nutraceuticals, and pharmaceuticals. Recently, numerous studies have investigated the functions of natural astaxanthin; however, the high cost of the production of astaxanthin from H. pluvialis cultures restricts its commercial viability. There is an urgent need to fulfill commercial demands by increasing astaxanthin accumulation from H. pluvialis cultures. In this study, we discovered that treatment of H. pluvialis cultures at the beginning of the macrozooid stage (day 0) with 1 μM rac-GR24, a synthetic analogue of strigolactones (a class of phytohormones), led to significant increases in biomass [up to a maximum dry cell weight (DCW) of 0.53 g/L] during the macrozooid stage and astaxanthin (from 0.63 to 5.32% of DCW) during the hematocyst stage. We elucidated that this enhancement of biomass accumulation during the macrozooid stage by rac-GR24 is due to its increasing CO2 utilization efficiency in photosynthesis and carbohydrate biosynthesis. We also found that rac-GR24 stimulated the overproduction of nicotinamide adenine dinucleotide phosphate (NADPH) and antioxidant enzymes in H. pluvialis cultures, which alleviated the oxidative damage caused by reactive oxygen species generated during the hematocyst stage due to the exhaustion of nitrogen supplies. Moreover, rac-GR24 treatment of H. pluvialis synergistically altered the activity of the pathways of fatty acid biosynthesis and astaxanthin esterification, which resulted in larger amounts of astaxanthin being generated by rac-GR24-treated cultures than by controls. In summary, we have developed a feasible and economic rac-GR24-assisted strategy that increases the amounts of biomass and astaxanthin generated by H. pluvialis cultures, and have provided novel insights into the mechanistic roles of rac-GR24 to achieve these effects.
研究领域:
植物学
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植物化学
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生物技术
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药理学
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DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 17
·4201
·
IF 4.2
Wenxuan Jiang,Xiangrong Deng,Lanxian Qin,Dahai Jiang,Mengqi Lu,Kai Chen,Manqi Yang,Liangliang Zhang,Jianchun Jiang,Liming Lu
This study focused on developing an effective cell wall-breaking method forPhaffia rhodozyma, followed by utilizing subcritical fluid extraction to isolate, extract, and concentrate astaxanthin from the complex fermentation products ofP. rhodozyma. A comprehensive comparison of seven distinct methods for disrupting cell walls, including dimethyl sulfoxide treatment, lactic acid treatment, sodium hydroxide treatment,β-glucanase enzymatic digestion,β-mannanase enzymatic digestion, and a combined enzymatic treatment involving bothβ-mannanase andβ-glucanase was conducted. The results identified the lactic acid method as the most effective in disrupting the cell walls ofP. rhodozyma. The software, Design Expert, was used in the process of extracting astaxanthin from cell lysates using a subcritical extraction method. Through fitting analysis and response surface optimization analysis by Design Expert, the optimal extraction conditions were determined as follows: an extraction temperature of 41 °C, extraction frequency of two times, and extraction time of 46 min. These parameters facilitated the efficient extraction, concentration, and enrichment of astaxanthin fromP. rhodozyma, resulting in an astaxanthin concentration of 540.00 mg/L. This result can establish the foundation for its high-value applications.
研究领域:
生物技术
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食品科学
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产品关联
DOI
PubMed
高影响因子
Antibiotics-Basel
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2021
·Vol. 10
·期 6
·681
·
IF 4.639
Gugulethu P. Khumalo,Nicholas J. Sadgrove,Sandy F. Van Vuuren,Ben-Erik Van Wyk
Indigenous trade of medicinal plants in South Africa is a multi-million-rand industry and is still highly relevant in terms of primary health care. The purpose of this study was to identify today’s most traded medicinal barks, traditionally and contemporaneously used for dermatological, gastrointestinal, and respiratory tract infections; then, to investigate the antimicrobial activity and toxicity of the respective extracts and interpret outcomes in light of pharmacokinetics. Thirty-one popularly traded medicinal barks were purchased from the Faraday and Kwa Mai-Mai markets in Johannesburg, South Africa. Information on the medicinal uses of bark-based medicines in modern commerce was recorded from randomly selected traders. The minimum inhibitory concentration (MIC) method was used for antimicrobial screening, and brine shrimp lethality was used to determine toxicity. New medicinal uses were recorded for 14 bark species. Plants demonstrating some broad-spectrum activities against tested bacteria includeElaeodendron transvaalense,Erythrina lysistemon,Garcinia livingstonei,Pterocelastrus rostratus,Rapanea melanophloeos,Schotia brachypetala,Sclerocarya birrea, andZiziphus mucronata. The lowest MIC value of 0.004 mg/mL was observed againstStaphylococcus epidermidisfor a dichloromethane bark extract ofE. lysistemon. The tested medicinal barks were shown to be non-toxic against theArtemia nauplii(brine shrimp) bioassay, except for a methanol extract fromTrichilia emetica(69.52% mortality). Bacterial inhibition of bark extracts with minimal associated toxicity is consistent with the safety and valuable use of medicinal barks for local muthi market customers. Antimicrobial outcomes against skin and gastrointestinal pathogens are feasible because mere contact-inhibition is required in vivo; however, MIC values against respiratory pathogens require further explaining from a pharmacokinetics or pharmacodynamics perspective, particularly for ingested rather than smoked therapies.Keywords:bark;antibacterial;traditional medicine;Zulu;brine shrimp lethality
研究领域:
药理学
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微生物学
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毒理学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yejun Zhang,Yongzhen Wang,Hao Dong,Jiao Li,Jianan Sun,Xiangzhao Mao
Temperature fluctuations can negatively affect the quality of frozen shrimp. Research on novel cryoprotectants to replace traditional agents (phosphate, etc.) has become a hotspot. Our results indicated that L-Proline could reduce thawing losses, delay texture deterioration and improve the functional properties of myofibrillar proteins of shrimp. Thawing loss in the proline group (3.2 %) was significantly lower than that in the control (5.4 %) after 3 freeze-thaw cycles ( p < 0.05). Compared to Na 4 P 2 O 7 , proline had better permeability and greater ability to inhibit ice crystal growth and volume expansion. Through molecular simulations, we found that proline might inhibit ice crystal formation by forming glassy states with water. Hydrogen bonding between proline and water/ice played a major role, and only a small amount of proline was required to significantly reduce the ice crystal growth rate from 0.16 m/s to 0.06 m/s. Briefly, proline exhibited potential as a cryoprotectant for shrimp in temperature fluctuations.
研究领域:
食品科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2025
·
IF 6.2
Zahra Essa Khan,Mengjiao Fan,Yuchen Jiang,Shu Zhang,Murtaza Khan,Qurshia Choudhry,Muhammad Rashid,Zanib Anwar,Sobia Kousar,Xun Hu
Herein, hydrothermal carbonization treatment of shrimp shells or together with glucose at 200 ºC plus subsequent activation with K 2 C 2 O 4 at 800 ºC was conducted, aiming to understand the retainment of organics and development of pore structures of resulting activated carbon. The results indicated that the organics in shrimp shells were highly unstable and the activation formed activated carbon with a mass yield of 5.6 %. Hydrothermal carbonization resulted in the leaching of some organics but promoted the aromatization of others, which in overall enhanced the resistivity of remaining organics towards cracking in activation, increasing the yield of activated carbon to 10.3 %. However, the leaching of a significant portion of aliphatic organics also decreased the specific surface area to 1128.1 m 2 /g (1593.2 m 2 /g from direct activation of shrimp shells. The co-hydrothermal carbonization of shrimp shells with glucose further increased activated carbon yield and enhanced the specific surface area of the activated carbon (1326.6 m 2 /g), due to additional aliphatic organics formed. Additionally, direct activation of shrimp shells formed activated carbon with dominant micropores (89.6 %), while hydrothermal carbonization of shrimp shells plus activation formed mainly mesopores (71.7 %). The presence of glucose in co-hydrothermal carbonization not only rendered micropores to dominate (80.3 %) but also retained appreciable amount of mesopores, forming activated carbon of superior capacity for the adsorption of methylene blue. Leaching of organics via the hydrothermal carbonization, promoted the formation of mesopores/macropores while suppressed the generation of micropores. Additionally, hydrothermal carbonization treatment resulted in wrapping of SiO 2 with organics, deterring further removal of SiO 2 via solid phase reactions with K 2 C 2 O 4 .
研究领域:
生物医学工程
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环境科学
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材料科学
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化学工程
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产品关联
DOI
高影响因子
BIOTECHNOLOGY LETTERS
·
2022
·Vol. 44
·期 4
·623-633
·
IF 2.716
Hao Zhi-kui,Li Jian-song,Wang Dan-hua,He Fei,Xue Jing-shi,Yin Liang-hong,Zheng Hua-bao
Objectives Shellfish waste is a primary source for making N -acetyl- d -glucosamine. Thus, establishing a high-efficiency and low-cost bioconversion method to produce N -acetyl- d -glucosamine directly from shellfish waste was promising.
Results A mutant C81 was obtained from Chitinolyticbacter meiyuanensis SYBC-H1 via 60 Co-γ irradiation. This mutant C81 showed the highest chitinase activity of 9.8 U/mL that was 85% higher than the parent strain. The mutant C81 exhibted improved antioxidant activities, including total antioxidant capacity, superoxide radical ability, and hydroxyl radical scavenging ability, compared to that of the parent strain. Four out of nine organic solvents increased the chitinase activity by 1.9%, 6.8%, 11.7%, and 15.8%, corresponding to methylbenzene, n-heptane, petroleum ether, and n-hexane, respectively. The biphase system composed of aqueous and hexane presented a five-fold reduction of cell viability compared to the control. Using a continuous fermentation bioconversion process, 4.2 g/L GlcNAc was produced from crayfish shell powder with a yield of 80% of the chitin content. Conclusions This study demonstrated that the mutant C81 is suitable for converting crayfish shell powder into GlcNAc in an aqueous-organic system.
研究领域:
微生物学
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生物化学
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生物工程
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2017
·
IF 4.529
Dan Xie,Jun Jin,Jiang Sun,Li Liang,Xiaosan Wang,Wei Zhang,Xingguo Wang,Qingzhe Jin
The effects of seven different extraction solvents (ethanol, isopropanol, acetone, ethyl acetate, isohexane, n-hexane, and subcritical butane) on the lipid yield and quality of the oil extracted from krill meal were investigated in this study. Phospholipids (PL), fatty acids (FA) composition and minor components including sterols, astaxanthin, vitamin A and tocopherols in the extracted krill oil were analyzed. The results indicated that ethanol and isopropanol led to comparatively higher lipid yields (16.33 and 14.52%, respectively) and PL contents (39.2 and 38.7%, respectively) but lower contents of the minor components than the other solvents. The krill oil extracted with acetone had the lowest PL content (20.63%) but contained more astaxanthin (206.74 mg/kg), vitamin A (27.84 mg/100 g), and sterols (39.00 mg/g). Moreover, high levels of n-3 FA were present in the extracts with high PL contents. Further analysis revealed that 23.65–28.10% of eicosapentaenoic acid (EPA) and 16.71–21.03% of docosahexaenoic acid (DHA) were present in the PL, while only 2.83–3.48% of EPA and 1.40–1.74% of DHA were detected in the triacylglycerols (TAG). In addition, subcritical butane proved to be an alternative to n-hexane and isohexane; krill oil extracted with these three solvents had similar qualities.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
ADSORPTION SCIENCE & TECHNOLOGY
·
2018
·
IF 0.609
Youzhou Zhou,Liuqin Ge,Neng Fan,Meisheng Xia
Two novel adsorbents derived from shrimp shell were prepared and their adsorption performances on Congo red were investigated. The results suggested that treated shrimp shell powder exhibited a higher adsorption capacity than raw shrimp shell powder. The factors of initial concentration, solution pH, adsorption time, and temperature were investigated. The maximum adsorption capacity of treated shrimp shell powder calculated according to the Langmuir isotherm model was 288.2 mg/g, which is much higher than that of chitin. The adsorption behavior could be fitted well by the pseudo-second-order kinetic model. Intra-particle diffusion model was also used to study the adsorption process. The thermodynamic parameters indicated the spontaneous and endothermic nature of the adsorption. Shrimp shell powder exhibited enough advantages such as large adsorption capacity, low cost, simple processing methods and high specific gravity compared with chitin or chitosan. This work confirmed that the shrimp shell biosorbent had a potential to be applied in dye wastewater treatment area.
研究领域:
环境科学
·
生物工程
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材料科学
·
化学工程
·
水污染控制
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DOI
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Ye Yuan,Xuexi Wang,Min Jin,Lefei Jiao,Peng Sun,Mónica B. Betancor,Douglas R. Tocher,Qicun Zhou
Lipid sources as alternatives to fish oil could alter the nutritional value and flavor quality of crab meat affecting consumer preferences. Herein, an 8-week nutritional trial was designed to investigate the effects of dietary lipid sources including fish oil (FO), krill oil (KO), palm oil, rapeseed oil, soybean oil and linseed oil on profiles of amino acids, fatty acids and volatiles in muscle of swimming crab ( Portunus trituberculatus ). Volatiles of crab muscle were characterized by headspace solid-phase microextraction and gas chromatography-tandem mass spectrometry. Results revealed that crabs fed FO and KO had significantly higher levels of protein, indispensable amino acids, eicosapentaenoic acid and docosahexaenoic acid in muscle. Principal component analysis and hierarchical cluster analysis demonstrated that muscle volatiles of crabs fed different dietary oils exhibited significant variations. Dietary FO and KO significantly increased the relative levels of 3-methylbutanal, heptanal, benzaldehyde and nonanal in muscle, which may produce more pleasant flavors.
研究领域:
水产养殖
·
营养学
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食品科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）