Food and Bioprocess Technology
·
2022
·Vol. 15
·期 12
·2762-2775
·
IF 5.581
Hu Li,Qiu Weiqiang,Feng Yuhui,Jin Yingshan,Deng Shanggui,Tao Ningping,Jin Yinzhe
The effect of recycling ohmic heating on the deacetylation, structure, and properties of chitosan prepared from Portunus trituberculatus crab shells was investigated. The effect of six proteases on deproteinization was examined. Deacetylation was carried out using four cycles of heating, in which heating methods included cyclic water bath heating, cyclic ohmic heating, and a combination of recycling ohmic and water bath heating. The structure and properties of chitosan were determined by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimeter (DSC), scanning electron microscope (SEM), dielectric properties, and rheological properties. A combination of many factors, including acid protease and pepsin, was chosen for complex proteolytic deproteinization. Ohmic heating took 2/5 of the time and used 1/2000 of the energy required for water bath heating. FTIR, DSC, dielectric properties, and SEM results showed that the chitosan characteristic after ohmic heating treatment met commercial requirements. Chitosan’s FTIR spectra and dielectric loss values at low frequencies after three cycles of combined ohmic and water bath heating were most similar to those of commercial chitosan without destroying its thermal stability. After the three cycles, the degree of chitosan deacetylation using combined ohmic and water bath heating reached 94.38 ± 0.21%, improving deacetylation compared with the traditional method.
研究领域:
材料科学
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生物技术
·
化学工程
·
分析化学
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Lin Zeng,Yong-Hong Wang,Chun-Xiang Ai,Bin Zhang,Hui Zhang,Zi-Ming Liu,Min-Hui Yu,Bing Hu
This study aims to evaluate the effects of oxytetracycline (OTC) on detoxification and oxidative defense in the hepatopancreas and intestine of Chinese mitten crab ( Eriocheir sinensis ) under cadmium (Cd) stress. The crab was exposed to 0.6 μM Cd, 0.6 μM OTC, and 0.6 μM Cd plus 0.6 μM OTC for 42 days. Our results showed that in the intestine, OTC alone enhanced protein carboxylation (PC) and malondialdehyde (MDA) contents, which was associated with the increased OTC accumulation. Compared to Cd alone, Cd plus OTC increased Cd and OTC contents, and reduced detoxification (i.e., glutathione (GSH) content, gene expressions of cytochrome P450 ( CYP ) isoforms, 7-ethoxyresorufin O-deethylase (EROD) activity, mRNA levels and activities of glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST)), and antioxidant defense (i.e., gene expressions and activities of catalase (CAT) and superoxide dismutase (SOD)) in the intestine, leading to the increased in PC and MDA contents, suggesting that OTC had a synergistic effect on Cd-induced oxidative damage. In the hepatopancreas, although OTC alone increased OTC accumulation, it did not affect PC and MDA contents. Compared to Cd alone, Cd plus OTC reduced MDA content, which was closely related to the improvement of detoxification (i.e., GSH content, mRNA levels of CYP isoforms, EROD activity, gene expressions and activities of GPx, GR and GST), and antioxidant defense (gene expressions and activities of CAT and SOD, metallothionein content). Aryl hydrocarbon receptor ( AhR ) and nuclear factor E2-related factor 2 ( Nrf2 ) transcriptional expressions were positively correlated with most detoxification- and antioxidant-related gene expressions, respectively, indicating that AhR and Nrf2 were involved in the regulation of these gene expressions. Our results unambiguously demonstrated that OTC had tissue-specific effects on Cd-induced toxicological effect in E. sinensis , which contributed to accurately evaluating Cd toxicity modulated by TCs in crab.
研究领域:
环境科学
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毒理学
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水产养殖学
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2022
·Vol. 2022
·期 1
·8348000
·
IF 3.781
Xuran Liu,Wenlong Wan,Mengge Li,Jiayuan Shi,Jie Xu,Zihan Zhou,Anran Wang,Shuyan Miao
Effects of dietary niacin on the growth performance, intestinal histomorphology, body composition, and antioxidant capacity were investigated in the present study to determine the optimum requirement of niacin for juvenile Eriocheir sinensis . All 360 crabs (initial average weight 1.14 ± 0.04 g) were randomly divided into 6 groups with 3 replicates in each group and 20 crabs in each replicate. Crabs were fed with the control diet (0.89 mg/kg) or niacin-supplemented diets (170.54 mg/kg, 347.05 mg/kg, 587.59 mg/kg, 784.85 mg/kg, and 1248.86 mg/kg) for 12 weeks (named as G1, G2, G3, G4, G5, and G6, respectively). The results showed that appropriate dietary niacin (above 347.05 mg/kg) significantly increased the weight gain rate (WGR) and specific growth rate (SGR) ( p < 0.05), but did not affect the survival rate (SR), feed conversion ratio (FCR), daily feeding rate (DFR), and molting frequency (MF) of crabs ( p > 0.05). The niacin content in the hepatopancreas of crabs in G1 and G2 was significantly lower than that of the other four groups ( p < 0.05). Moreover, dietary niacin significantly affected the intestinal histomorphology of crabs, including the number of folds (NF), height of folds (HF), height of microvillus (HMV), and thickness of muscularis (TM) ( p < 0.05). Additionally, moderate dietary niacin levels significantly affected the nonspecific immune responses of crabs, by improving the activity of catalase (CAT), glutathione s-transferase (GST), glutathione peroxidase (GSH-Px), and total superoxide dismutase (T-SOD) ( p < 0.05). Based on the broken-line model analysis of SGR against dietary niacin level, the dietary niacin requirement of juvenile crabs was suggested to be 419.4 mg/kg.
研究领域:
水产养殖学
·
营养学
✓ 已验证
DOI
高影响因子
Waste Disposal & Sustainable Energy
·
2024
·Vol. 6
·期 2
·173-183
·
IF 3.6
Li Yiming,Yu Zhihao,Guo Mengyan,Zhang Ming,Xiong Jian,Qiao Yina,Zhang Rui,Lu Xuebin
Biomass-based carbon materials with hierarchical porous structures have attracted attention for their ability to provide more channels and shorten ion transport paths. Here, we developed a simple method based on confined nanospace deposition. During high-temperature treatment, the mesoporous silica layer wrapped around the outside of the crab shells acted as a closed nanospace and effectively suppressed the severe deformation of the crab shell structure by shrinking inward. The prepared carbon material has a layered porous structure with abundant and stable N and O co-doping (N 7.32%, O 3.69%). The specific capacitance of the three-electrode system was 134.3 F/g at a current density of 0.5 A/g in a 6 mol/L KOH electrolyte, and the assembled aqueous symmetric supercapacitors exhibited an excellent cycling stability of 98.81% even after 5000 cycles. Graphical abstract
研究领域:
材料科学
·
能源存储
·
生物质利用
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产品关联
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 8
·1287
·
IF 5.1
Siqi Zhou,Renyue Zhang,Zehui Qiu,Yuyao Shi,Shaicheng Zhu,Xugan Wu,Xichang Wang,Long Zhang
This study investigated the effects of dietary Antarctic krill meal (AKM) on the physiological metabolism and flavor quality of adultEriocheir sinensisovaries during the postharvest temporary rearing. The AKM concentrations tested were 0% (including negative control group and positive control group), 2%, 4%, 6%, and 8%. The results indicate that theE. sinensisovaries in 8% AKM group produced the highest levels of aroma compounds after thermal processing, including hexanal, heptanal, phenylacetaldehyde, 3-octanone, and 2-methylbutanoic acid ethyl ester. The 8% AKM and negative control group were analyzed by UPLC-MS/MS combined with the nontargeted and widely targeted metabolomics technique. The AKM altered the composition of aroma precursors by adjusting the metabolism of glycerophospholipid, linoleic acid, α-linolenic acid, and amino acid in ovaries. Moreover, lipids composed of polyunsaturated fatty acids (PUFAs) were significantly upregulated (p< 0.05). Dietary supplementation with 8% AKM had the best effect on improving the ovarian flavor quality ofE. sinensis. During the postharvest temporary rearing, more aromatic precursors were produced by regulating physiological metabolism. The ovarian flavor was enhanced by lipid oxidation, Maillard reaction, and Strecker degradation during thermal processing.
研究领域:
水产养殖学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Zhicheng Cai,Jianfeng Lu,Ziqi Dong,Hong Wang,Qiang Li,Lin Lin,Chenghui Wang,Bin Zheng,Xiangzhao Mao,Changhu Xue
Chitin, a high-value biopolymer derived from crustacean shells, generally requires harsh chemical decolorization to achieve acceptable purity and appearance. Herein, we developed a green extraction method for chitin using atmospheric pressure plasma jet pretreatment combined with acid–base processing. Chinese mitten crab shells were subjected to different sequences and concentrations of hydrochloric acid (HCl) and sodium hydroxide (NaOH) treatments, with and without plasma pretreatment. Structural and physicochemical characterizations were performed via FTIR, SEM–EDS, AFM, XPS, NMR, XRD, TGA, and molecular weight analysis. The results indicated that when the acid–base concentrations were ≥2-M HCl and ≥1-M NaOH, plasma pretreatment (PJ–HN) enabled the release of high-purity chitin without the need for chemical decolorization. The chitin obtained exhibited comparable crystallinity (64.58 %), thermal stability (DTG max = 402.9 °C), and molecular weight (6.89 × 10 5 g/mol) to commercial chitin. Moreover, the PJ–HN samples retained high acetylation degrees and showed enhanced surface roughness, indicating improved functional potential. The antibacterial test confirmed that it possesses broad-spectrum antibacterial activity. The present study demonstrates that plasma-assisted extraction can serve as an eco-friendly and efficient alternative to conventional chemical methods in crustacean chitin processing.
研究领域:
生物高分子
·
绿色化学
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材料科学
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生物技术
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
Food Biophysics
·
2022
·Vol. 17
·期 4
·484-494
·
IF 3.34
Liu Jinming,Xu Jianhang,Chen Qian,Ren Jing,Wang Hui,Kong Baohua
Abstract PVA is a kind of biodegradable polymer that can be used as carbon source and energy by bacteria. In this study, deacetylated crab-shell particles (dCSPs) which exhibit strong biological activity due to the composition of chitin and CaCO 3 , as natural and environmentally friendly multi-macromolecule fillers, were used to modify poly(vinyl alcohol) (PVA) resin to form PVA/dCSP biocomposites. The Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) results proved that the –OH in PVA and the C=O groups of CO 3 2- in the dCSPs could form hydrogen bondings, which might retard the crystallisation of PVA. With the increased of dCSPs content, those biocomposites showed improved surface hydrophobicity, water vapor barrier, thermomechanical properties, thermostability, fire resistance, and antibacterial activity. Especially, PVA/dCSP biocomposite film with 30% dCSPs showed excellerent properties above with not much sacrifice of tensile strength and elongation at break. The incorporation of dCSPs effectively enhanced the bacteriostasis ability against both Escherichia coli and Staphylococcus aureus . The introduction of dCSPs in PVA ensures effective utilisation of agricultural by-products. In general, this study provides an alternative method for the development of biodegradable antibacterial materials, which could be used in food packaging applications. Graphical Abstract
研究领域:
材料科学
·
生物学
·
食品科学
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产品关联
DOI
高影响因子
Antioxidants
·
2022
·Vol. 11
·期 10
·2029
·
IF 7.675
Wenrong Feng,Shengyan Su,Changyou Song,Fan Yu,Jun Zhou,Jianlin Li,Rui Jia,Pao Xu,Yongkai Tang
High concentrations of copper (Cu2+) pose a great threat to aquatic animals. However, the mechanisms underlying the response of crustaceans to Cu2+exposure have not been well studied. Therefore, we investigated the alterations of physiological and molecular parameters in Chinese mitten crab (Eriocheir sinensis) after Cu2+exposure. The crabs were exposed to 0 (control), 0.04, 0.18, and 0.70 mg/L of Cu2+for 5 days, and the hemolymph, hepatopancreas, gills, and muscle were sampled. The results showed that Cu2+exposure decreased the antioxidative capacity and promoted lipid peroxidation in different tissues. Apoptosis was induced by Cu2+exposure, and this activation was associated with the mitochondrial and ERK pathways in the hepatopancreas. ER stress-related genes were upregulated in the hepatopancreas but downregulated in the gills at higher doses of Cu2+. Autophagy was considerably influenced by Cu2+exposure, as evidenced by the upregulation of autophagy-related genes in the hepatopancreas and gills. Cu2+exposure also caused an immune response in different tissues, especially the hepatopancreas, where the TLR2-MyD88-NF-κB pathway was initiated to mediate the inflammatory response. Overall, our results suggest that Cu2+exposure induces oxidative stress, ER stress, apoptosis, autophagy, and immune response inE. sinensis, and the toxicity may be implicated following the activation of the ERK, AMPK, and TLR2-MyD88-NF-κB pathways.Keywords:copper;apoptosis;endoplasmic reticulum stress;oxidative stress;Eriocheir sinensis
研究领域:
水生毒理学
·
分子生物学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Hui Wang,Jinming Liu,Xu Fan,Jing Ren,Qian Liu,Baohua Kong
In this study, natural crab-shell particles (CSP) and deacetylated crab-shell particles (dCSP) were used as crosslinking agents at the present of glucolactone to obtain high strength mono-crosslinked and bi-crosslinked sodium alginate (NaAlg) hydrogel films, respectively. The results indicated that the CSP in the system could occur Ca 2+ crosslinking, and the addition of dCSP could help to form both Ca 2+ crosslinking and polyelectrolytes interactions with NaAlg. The remaining unreacted CaCO 3 could enhance the thermal stability of the films due to the high thermally stable CaCO 3 . The surface hydrophobicity, gel properties, mechanical strength and thermal stabilities of those bi-crosslinked NaAlg hydrogel films were better than those of neat NaAlg film and mono-crosslinked NaAlg hydrogel films, which overcome the inferior thermo-mechanical properties for traditional hydrogel films. In addition, the releasing time of those prepared hydrogel films for diclofenac sodium was higher than 36 h, which illustrated sustained drug releasing activity. The CSP modified NaAlg hydrogel film with 0.5% GDL content exhibited the highest cumulative release amount. The proposed approach is a promising green crosslinking technique to develop high performance NaAlg hydrogel films that can realize active ingredients and drugs sustained release in food packaging and medical wound dressing areas.
研究领域:
材料科学
·
药学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2016
·
IF 3.847
Jean-Charles Gautier,Xiaobing Zhou,Yi Yang,Thierry Gury,Zhe Qu,Xavier Palazzi,Jean-François Léonard,Mohamed Slaoui,Yaligara Veeranagouda,Isabelle Guizon,Eric Boitier,Aziz Filali-Ansary,Bart H.J. van den Berg,Oliver Poetz,Thomas Joos,Tianyi Zhang,Jufeng Wang,Philippe Detilleux,Bo Li
Most studies to evaluate kidney safety biomarkers have been performed in rats. This study was conducted in Cynomolgus monkeys in order to evaluate the potential usefulness of novel biomarkers of nephrotoxicity in this species. Groups of 3 males were given daily intramuscular injections of gentamicin , a nephrotoxic agent known to produce lesions in proximal tubules, at dose-levels of 10, 25, or 50 mg/kg/day for 10 days. Blood and 16-h urine samples were collected on Days − 7, − 3, 2, 4, 7, and at the end of the dosing period. Several novel kidney safety biomarkers were evaluated, with single- and multiplex immunoassays and in immunoprecipitation-LC/MS assays, in parallel to histopathology and conventional clinical pathology parameters. Treatment with gentamicin induced a dose-dependent increase in kidney tubular cell degeneration/necrosis, ranging from minimal to mild severity at 10 mg/kg/day, moderate at 25 mg/kg/day, and to severe at 50 mg/kg/day. The results showed that the novel urinary biomarkers, microalbumin, α1-microglobulin, clusterin , and osteopontin , together with the more traditional clinical pathology parameters, urinary total protein and N-acetyl-β-D-glucosaminidase (NAG), were more sensitive than blood urea nitrogen (BUN) and serum creatinine (sCr) to detect kidney injury in the monkeys given 10 mg/kg/day gentamicin for 10 days, a dose leading to an exposure which is slightly higher than the desired therapeutic exposure in clinics. Therefore, these urinary biomarkers represent non-invasive biomarkers of proximal tubule injury in Cynomolgus monkeys which may be potentially useful in humans.
研究领域:
毒理学
·
药理学
·
临床研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENERGY
·
2023
·
IF 9
Yunchao Li,Yangkai Sun,Hanming Li,Mengwei Sun,Jie Shen,Shurong Wang
In this study, we report a unique, facile process for the preparation of a high-nitrogen-oxygen dual-doped three-dimensional (3D) hierarchical porous carbon network by one-step pyrolysis and in situ activation under a CO 2 atmosphere combined with acid treatment using Eriocheir sinensis as the precursor. The evolution of the physicochemical structure of carbon at different pyrolysis temperatures was investigated. Removing inorganics from the shell carbon of Eriocheir sinensis enabled the formation of a unique 3D network structure. In addition, a high doping concentration of nitrogen and oxygen can be formed in the porous carbon skeleton. The increase in the pyrolysis temperature was beneficial to the pore development and graphitisation of carbon. Because of the interconnected 3D hierarchical porous structure and high heteroatom doping content, which provides fast channels for electron and ion transport, the obtained carbon materials exhibit excellent capacitive performance as supercapacitor electrodes. Quantum chemical calculations further confirmed that the heteroatom-doped carbon plane has a larger negative charge and stronger interaction with K + than that of the pure carbon surface. This inexpensive green carbon material based on food waste is expected to be a candidate material for high-performance electrode precursors.
研究领域:
材料科学
·
能源存储
✓ 已验证
产品关联
DOI
高影响因子
Cell Reports Medicine
·
2025
·
IF 10.6
Raoxian Bai,Wenting Guo,Ting Zhang,Shuaiwei Ren,Jie Liu,Puhao Xiao,Junyu Zhang,Wenjie Sun,Jiao Yang,Yue Ma,Siyu Liu,Chaoran Zhou,Shangang Li,Hong Wang,Shu Zhang,Weizhi Ji,Shiwen Wu,Yongchang Chen
Progress in Duchenne muscular dystrophy (DMD) treatment is hindered by the lack of animal models that closely replicate human pathology and enable the evaluation of therapy efficacy and safety based on these models. To address this need, we optimize the generation of nonhuman primate DMD models, reducing the development time from 6 to 7 years to under 1 year, enabling the rapid generation of DMD monkey models. These models closely mimic human DMD pathology and motor dysfunction, making them suitable for testing gene therapies. Using these models, we develop a single-cut gene therapy strategy that can be directly applied to humans. This treatment restores dystrophin expression, improves pathological features, and enhances motor abilities in DMD monkeys, with effects lasting at least 1.5 years. In conclusion, we achieve the rapid generation of DMD monkey models and demonstrate that our gene therapy approach is effective and holds significant potential for clinical application.
研究领域:
医学遗传学
·
基因治疗
·
实验动物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）