ACS Applied Bio Materials
·
2025
·
IF 4.7
Shujie Wu,Shiwen Yang,Linlin Ou,Hongjian Zhang,Lu Wang,Bingyu Feng,Zeyu Bai,Weichang Li,Bin Cheng,Wei Seong Toh,Juan Xia
Circadian rhythm disruption, commonly caused by factors such as jet lag and shift work, is increasingly recognized as a critical factor impairing wound healing. Although melatonin is known to regulate circadian rhythms and has potential in wound repair, its clinical application is limited by low bioavailability. To address these challenges, we developed an alginate-based dual-network hydrogel as a delivery system for melatonin, ensuring its stable and sustained release at the wound site. This approach enhances the efficacy of melatonin in modulating the wound healing process. We investigated the effects of circadian rhythm disruption on the wound microenvironment under the influence of the melatonin-loaded hydrogel with a focus on its biocompatibility, hemostatic properties, and antioxidant response functions. Additionally, we elucidated the mechanisms by which the melatonin-loaded hydrogel system promotes wound healing. Our findings provide insights into the relationship between circadian rhythm disruption and wound healing, offering a promising strategy for the management of chronic wounds associated with circadian rhythm disorders.
研究领域:
生物医学工程
·
药理学
·
组织修复与再生
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产品关联
DOI
PubMed
高影响因子
Carbon Letters
·
2023
·Vol. 33
·期 7
·2129-2139
·
IF 4.5
Tang Shanshan,Liang Axin,Liu Miao,Wang Wei,Zhang Fulai,Luo Aiqin
A glassy carbon electrode modified with a composite consisting of electrodeposited chitosan and carboxylated multi-walled carbon nanotubes (e-CS/MWCNTs/GCE) was used as a working electrode for simultaneous determination of dopamine (DA), serotonin (5-HT) and melatonin (MT), which were related to circadian rhythms. The electrochemical characterizations of the working electrode were carried out via electrochemical impedance spectroscopy and chronocoulometry. It was found that electrochemical modification method, that was cyclic voltammetry, may can cause continuous CS polymerization on MWCNTs surface to form a dense membrane with more active sites on the electrode, and the electrochemically active surface area of e-CS/MWCNTs/GCE obtained was about 7 times that of GCE. The electrochemical behaviour of DA, 5-HT and MT on working electrode were carried out via differential pulse voltammetry and cyclic voltammetry. The results showed that e-CS/MWCNTs/GCE solved the problem that the bare electrode could not detect three substances simultaneously, and can catalyze oxidation potential difference as low as 0.17 V of two substances reaction at the same time, indicating very good electrocatalytic activity. By optimizing the detection conditions, the sensor showed a good linear response to DA, 5-HT and MT in the range of 20-1000 μmol/L, 9-1000 μmol/L and 20-1000 μmol/L, and the detection limits were 12 μmol/L, 10 μmol/L and 22 μmol/L (S/N = 3), respectively. In addition, the proposed sensor was successfully applied to the simultaneous detection of DA, 5-HT and MT in human saliva samples.
研究领域:
分析化学
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生物医学工程
·
电化学
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DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2020
·
IF 4.872
Guangjing Ma,Meide Zhang,Jilei Xu,Wuxian Zhou,Liwen Cao
Heat stress has been a major environmental factor limiting the growth and development of Pinellia ternata which is an important Chinese traditional medicine. It has been reported that spermidine (SPD) and melatonin (MLT) play pivotal roles in modulating heat stress response (HSR). However, the roles of SPD and MLT in HSR of P. ternata , and the potential mechanism is still unknown. Here, exogenous SPD and MLT treatments alleviated heat-induced damages in P. ternata , which was supported by the increased chlorophyll content, OJIP curve, and relative water content, and the decreased malondialdehyde and electrolyte leakage. Then, RNA sequencing between CK (control) and Heat (1 h of heat treatment) was conducted to analyze how genes were in response to short-term heat stress in P. ternata . A total of 14,243 (7870 up- and 6373 down-regulated) unigenes were differentially expressed after 1 h of heat treatment. Bioinformatics analysis revealed heat-responsive genes mainly included heat shock proteins ( HSPs ), ribosomal proteins, ROS-scavenging enzymes, genes involved in calcium signaling, hormone signaling transduction, photosynthesis, pathogen resistance, and transcription factors such as heat stress transcription factors ( HSFs ), NACs , WRKYs , and bZIPs . Among them, PtABI5 , PtNAC042 , PtZIP17 , PtSOD1 , PtHSF30 , PtHSFB2b , PtERF095 , PtWRKY75 , PtGST1 , PtHSP23.2 , PtHSP70 , and PtLHC1 were significantly regulated by SPD or MLT treatment with same or different trends under heat stress condition, indicating that exogenous application of MLT and SPD might enhance heat tolerance in P. ternata through regulating these genes but may with different regulatory patterns. These findings contributed to the identification of potential genes involved in short-term HSR and the improved thermotolerance by MLT and SPD in P. ternata , which provided important clues for improving thermotolerance of P. ternata .
研究领域:
植物学
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分子生物学
·
药用植物学
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Hangyue Zhao,Lei Wang,Tarun Belwal,Yunhong Jiang,Dong Li,Yanqun Xu,Zisheng Luo,Li Li
This work was designed to develop the chitosan-based melatonin layer-by-layer assembly (CMLLA) via the inclusion method. The structural characterizations and interaction present in CMLLA were investigated by the scanning electron microscope (SEM), X-ray diffraction (XRD) and Fourier Transform-Infrared spectroscopy (FTIR). The ratio of chitosan (CH) to carboxymethylcellulose (CMC) greatly influenced the mechanical properties, including the tensile strength, moisture content and color performance. Results showed that both antioxidant and antimicrobial properties of CMLLA were enhanced with the addition of melatonin (MLT). Furthermore, it was demonstrated that the CMLLA with 1.2 % (w/v) CH, 0.8 % (w/v) CMC and 50 mg/L MLT better contributed to the delay of chlorophyll degradation and the maintenance of shelf-life quality. Results from this study might open up new insights into the approaches of quality improvement of postharvest fresh products by incorporating the natural antioxidant compounds into natural polymers.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 35
·16337-16344
·
IF 3.3
Shanshan Tang,Miao Liu,Wei Wang,Axin Liang,Fulai Zhang,Aiqin Luo
A novel electrochemical sensor, wherein the working electrode is a glassy carbon electrode modified with amorphous zinc/copper double transition metal hydroxide (ZnCu-TMH/GCE), is developed for simultaneous detection of dopamine (DA), serotonin (5-HT), and melatonin (MT), which is important for the understanding and treatment of circadian rhythm disorder diseases. The working electrode was studied by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and differential pulse voltammetry (DPV). It showed that amorphous ZnCu-TMH can significantly improve the oxidation of DA during the simultaneous detection of DA, 5-HT, and MT, and the electrochemical peak potential difference was as low as 0.15 V between DA and 5-HT, showing very good electrocatalytic performance. By optimizing the detection conditions, the sensor showed a good linear response to DA, 5-HT, and MT in the range of 3 × 10−6 mol L−1 ∼ 1 × 10−3 mol L−1 (with two linear relationship curves), and the detection limits were 1.048 × 10−6, 2.213 × 10−6, and 2.356 × 10−6 mol L−1 (S/N = 3), respectively. In addition, ZnCu-TMH/GCE was successfully applied to the simultaneous detection of DA, 5-HT, and MT in human saliva samples.
研究领域:
分析化学
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生物医学工程
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DOI
高影响因子
PHARMACOLOGICAL RESEARCH
·
2021
·
IF 7.658
Yuqian Jia,Bingqing Yang,Jingru Shi,Dan Fang,Zhiqiang Wang,Yuan Liu
The widespread dissemination of antibiotic resistance genes (ARGs) is a serious problem and constitutes a threat for public health. Plasmid-mediated conjugative transfer of ARGs is recognized as one of the most important pathways accounting for this global crisis. Inhibiting the conjugative transfer of resistant gene-bearing plasmids provides a feasible strategy to prevent the spread of antibiotic resistance. Here we found that melatonin , a neurohormone secreted from pineal gland, substantially inhibited the horizontal transfer of RP4–7 plasmid in a dose-dependent manner. Furthermore, melatonin could also suppress the conjugal frequency of different types of clinical plasmids that carrying colistin resistance gene mcr-1 rather than bla NDM or tet (X) genes. Next, we investigated the mechanisms underlying the inhibitory effect of melatonin on conjugation. As a result, we showed that the addition of melatonin markedly reduced bacterial membrane permeability and inhibited the oxidative stress. In line with these observations, the conjugative transfer-related genes were regulated accordingly. Most importantly, we uncovered that melatonin disrupted bacterial proton motive force (PMF), which is an essential bacterial energy metabolism substance and is important for conjugative process. Collectively, these results provide implications that some non-antibiotics such as melatonin are effective inhibitors of transmission of ARGs and raise a promising strategy to confront the increasing resistant infections.
研究领域:
微生物学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2022
·
IF 4.622
Zhiying Miao,Zhiruo Miao,Xiaohua Teng,Shiwen Xu
Lead (Pb) has been concerned as one of the most severe hazardous contaminants, because it can cause pyroptosis in multiple tissues of mammals and birds. Melatonin (Mel) has attracted much interest for its role in governing intestinal injury via microRNAs (miRNAs). To explore the effect of Mel on Pb exposure-induced intestinal epithelial cell pyroptosis in common carps by regulating miR-17-5p/TXNIP axis, the Pb exposure and Pb-Mel treated models were constructed in vivo . The results elucidated that the suppressed expression of miR-17-5p and intensified level of TXNIP were primarily detected in Pb-exposed gut tissues, and both abolished with Mel addition, along with downregulated Pb-mediated elevated expression of NLRP3, CASP1, IL1β and GSDMD. Additionally, the targeting relationship between miR-17-5p and TXNIP were demonstrated by dual-luciferase reporter assay, and on this basis, miR-17-5p NC, mimic and inhibitor cell models were established. Thereby, Thereby, the expression of TXNIP in the miR-17-5p mimic groups was significant lower in the Pb-exposure but still elevated than the Control group, and the expression of NLRP3 and NLRP3-dependent pyrotposis-related genes performed consistent alterations. Noticeably, the expression of TXNIP suppressed with Mel addition even in the miR-17-5p inhibitor cell model, resulting in the inactivation of NLRP3 inflammasome-dependent pyroptosis. Overall, we draw the conclusion as Mel attenuates Pb-induced intestinal epithelial cell pyroptosis via miR-17-5p/TXNIP axis. The present study provides a novel perspective for toxicological mechanism of Pb, and new insights for the detoxification mechanism of Mel.
研究领域:
毒理学
·
分子生物学
·
水生生物学
·
环境科学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Shuangshuang Tian,Liqiang Chen,Jinghua Zhang,Hong Zhang,Liangquan Sheng,Xinxin Wang,Deqian Huang
Carbon nitride nanoflower materials (CNNFs) modified electrodes were prepared and used as electrochemiluminescence (ECL) sensors for the sensitive detection of melatonin (MT) in food. The luminescence intensity of CNNFs is increased by 4.6 times compared with bulk g-C 3 N 4 . In addition, the effect of dissolved oxygen on the material was eliminated, and the stability of ECL intensity of CNNFs was improved. Under the optimal experimental conditions, there is a good linear relationship between the ECL intensity ratio and logC MT in a concentration range of 2.0 × 10 −11 –1.0 × 10 −6 mol/L, and the detection limit is 6.2 × 10 −13 mol/L. This experiment has been successfully used for the detection of MT in rice, black rice , oats, apples, bananas, grapes, carrots, tomatoes, cucumbers, bread, and beers. The results are consistent with those obtained by high-performance liquid chromatography (HPLC). Therefore, this sensor is a sensitive and effective method for detecting MT content in food.
研究领域:
分析化学
·
食品安全
·
生物传感
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产品关联
DOI
PubMed
高影响因子
Carbon Letters
·
2023
·Vol. 34
·期 1
·437-444
·
IF 4.5
Sun Bin,Sun Wei,Wang Zhigang,Zhao Bo,Yang Song
In this paper, a simple, cost-effective, and efficient electrochemical sensor for molecular imprinting melatonin was established. The molecular imprinted films were formed by in situ electrochemical polymerization using molecular imprinting technology. The modification method, modification time and other parameters of the electrode were optimized. Under optimized conditions, the sensor responds to melatonin concentration in a linear range of 0–100 μM. The detection limit was 0.171 μM. In addition, the sensor has little response to interfering substances, such as uric acid, vitamin B6, vitamin C, and glucose, and can be tested in real samples. The recoveries were 98.73–101.60%.
研究领域:
分析化学
·
生物医学工程
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DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Huilian Yu,Saif Ali,Ke Yan,Wenqing Zhao,Shanshan Wang,Zhiguo Zhou,Wei Hu
Drought stress negatively affects cotton pollen fertility, which in turn leads to a decrease in seed number per boll and boll weight. Exogenous melatonin application significantly enhances pollen fertility under drought conditions, while the specific underlying mechanisms remain unclear. A pot experiment was conducted using a cultivar Yuzaomian 9110 under two moisture treatments (soil relative water content at 75 ± 5 % and 45 ± 5 %) with two melatonin concentration (0 and 200 μM) to investigate the effects of exogenous melatonin on the structural traits and physiological metabolism of cotton anthers and its’ relationships with pollen fertility. Results demonstrated the significant impact of drought on anthers development and metabolism, with damage to the anther tapetum and decreased starch and adenosine triphosphate (ATP) contents, subsequently resulting in reduced pollen germination rate, seed number per boll and boll weight. Melatonin application in water-deficit anthers up-regulated the expression of sucrose transporter protein ( GhSWEET55 ) and phosphate sucrose synthetase, promoting sucrose import and synthesis, respectively. However, it also increased sucrose synthase and acid convertase, accelerating sucrose decomposition and reducing its content. Additionally, melatonin application promoted starch accumulation in water-deficit anthers by enhancing activities of adenosine diphosphate glucose pyrophosphorylase and soluble starch synthase, meaning that potential energy storage was increased, which facilitated the formation of pollen fertility. Although melatonin application reduced the expression of pyruvate kinase ( GhPK ) and glucose 6-phosphogluconate dehydrogenase ( GhG6PD ) genes in water-deficit anthers, it up-regulated hexokinase ( GhHXK ) and citrate synthase ( GhCIT ) expression, enhancing ATP content, and ultimately pollen fertility, seed number and boll weight under drought. In summary, exogenous melatonin preserved cotton pollen fertility under drought stress by regulating carbohydrate and energy metabolism, especially enhancing starch and ATP accumulation in anthers.
研究领域:
植物生理学
·
农业科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiali Guan,Gang Zhou,Yubo Fan
Melatonin (MT) is a hormone regulating the sleep-wake cycle and serves as a potential biomarker for various physiological and pathological conditions. Traditional MT detection methods, such as HPLC and MS, face challenges including complex processing, low sensitivity, and the inability to provide real-time monitoring, limiting their clinical applicability. Electrochemical sensors offer advantages like simplicity, high sensitivity, and real-time detection; however, issues such as insufficient selectivity, poor stability, and limited reproducibility in complex biological matrices persist. To address these challenges, we developed an Ab-MEL/AR/ITO electrochemical sensor by integrating AgNPs@RGO (AR) composites, synthesized using a dopamine in situ reduction method, with the MEL-1A-R (B-10) antibody (Ab-MEL) as a selective molecular recognition element for MT detection. The sensor exhibited a linear response to MT concentrations ranging from 0.01 to 1000 μM, with an exceptionally low limit of detection (LOD) of 0.0016 μM. It demonstrated excellent selectivity, maintaining performance in the presence of interferents like NaCl, glucose, and dopamine, and exhibited high reproducibility and long-term stability, with only a 5.68 % decrease in response after 28 days. The sensor achieved recovery rates of 98.10 % to 106.30 % in synthetic urine, human serum, and commercial MT capsules, underscoring its robustness and precision. By addressing the limitations of conventional methods, this Ab-MEL/AR/ITO sensor provides a highly sensitive, selective, and stable platform for MT detection, offering significant potential for clinical diagnostics and biomedical research applications.
研究领域:
分析化学
·
生物医学工程
·
临床诊断
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产品关联
DOI
高影响因子
CRYSTAL GROWTH & DESIGN
·
2024
·
IF 3.2
Shuang Xiao,Chunyan Wang,Teng You,Ping Huang,Qiuxia Deng,Ping Jiang,Peng Chen,Daiping He
It is highly desirable but still remains challenging to tune nanozymes with superior catalytic activity via simple operation. Herein, the oxidase-like activity of Ru nanoparticles supported on porous carbon (Ru/PC) was significantly modulated via a facile partial oxidation process. Due to the small size, uniform dispersion, high electron density on the surface, as well as abundant interfacial heterostructure between Ru and RuO2, the partially oxidized Ru/PC (P–Ru/PC) showed excellent oxidase-mimicking activity in triggering 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation, with a low Km of 0.138 mM and a high Vmax of 2.82 × 10–7 M·s–1. Theoretical calculations reveal that both the absorption and subsequent activation of molecular oxygen are more favorable on the interfacial heterostructure of P–Ru/PC than on the Ru/PC surface. Moreover, the oxidase-like activity of P–Ru/PC was found to be suppressed by melatonin, along with a fading color of oxidized TMB (TMBox). As a result, a novel colorimetric system consisting of P–Ru/PC and TMB was established for melatonin detection for the first time, which shows a wide linear range of 10–220 μM and a low detection limit of 0.023 μM. This work not only provides a simple but smart strategy of interface engineering to tune the oxidase-like activity of Ru/PC, but also expands the application of Ru oxidase mimics in pharmaceutical analysis.
研究领域:
纳米材料科学
·
生物分析化学
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药学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）