MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Huijun Guo,Jie Chen,Siqi Li,Yifei Qi,Guoxin Wang,Yue Zhang
In this work, a ratiometric double signals electrochemical sensing platform was constructed for creatinine (Cre) analysis employing silver nanoparticles (AgNPs)@ MXenes/thionine acetate (THAT) composites. The synthesis of MXenes/THAT composites was utilized to modify on the surface of a screen-printed electrode (SPE) via dripping coating, followed by the electro-deposition of AgNPs. While the anodic current of the AgNPs decreased in the presence of Cre due to the competive reaction for forming metal complexes, the current of THAT remained invariant, which attained a ratiometric response. Under optimized conditions, the response current ratio ( I Ag /I THAT ) decreases while the concentration of Cre increased linearly between 0 ∼ 1.2 mM, with 0.01 μM as a adaptive detection limit. It provided a new sensing platforming for employing double-signals nanostructures with remarkable stability and sensitivity to design ratiometic sensors for analysis of Cre in human urine samples with real-time.
研究领域:
分析化学
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2016
·
IF 2.567
Hong Du,Ruiyi Chen,Jianjun Du,Jiangli Fan,Xiaojun Peng
A gold nanoparticle-based colorimetric sensor for the determination of creatinine was developed as an important index for early diagnosis of kidney function and corresponding renal diseases. Because of the unique synergistic coordination capability of adenosine and creatinine with Ag+ on a particle surface, our system exhibits an excellent selectivity to creatinine among various ions and biomolecules. There are good linear relationships of absorption changes (A630 nm/520 nm) over creatinine concentrations, so both colorimetric qualitative detection by the naked eye and quantitative determination by UV–vis spectrometer could be realized with an excellent limit of detection compared with that of other methods. Finally, by testing creatinine in practical samples, such as urine mimic and bovine serum, good recoveries were obtained with proper relative standard deviations.
研究领域:
生物化学
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医学诊断
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
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2016
·
IF 4.219
Hongying Su,Qingming Jia,Shaoyun Shan
Polysaccharide-based microgels with high water content, excellent biocompatibility and controllable particle size have been widely studied as ideal candidates for drug release and delivery. In this study, microgels based on dextran were developed via the Schiff base formation between aldehyded dextran and ethylenediamine in a water-in-oil (W/O) microemulsion. Particle size of the resulted microgel was controllable between 800 and 1100 nm by modulating the amount of the employed co-surfactants (Span 80/Tween 80). Furthermore, fluoresceins ( e.g. , aminofluorescein) and drugs ( e.g. , doxorubicin) with free amino groups can be conjugated onto the network of the dextran-based microgel via Schiff base linkages. Since the Schiff base linkages are degradable via hydrolysis and their stability decreases with the environmental pH decreases, the resulted Schiff bases contained microgel showed a pH dependent degradation profile. These results indicated that the pH-sensitive microgel based on dextran could be used as promising drug delivery systems for biomedical applications.
研究领域:
生物材料
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
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IF 7.7
Xiaoqiang Wang,Menghan Li,Zhiyuan Liu,Zhuang Shi,Daoyong Yu,Baosheng Ge,Fang Huang
Utilizing carbonic anhydrase (CA) to catalyze CO 2 hydration offers a sustainable and potent approach for carbon capture and utilization. To enhance CA's reusability and stability for successful industrial applications, enzyme immobilization is essential. In this study, delignified bamboo cellulose served as a renewable porous scaffold for immobilizing CA through oxidation-induced cellulose aldehydation followed by Schiff base linkage. The catalytic performance of the resulting immobilized CA was evaluated using both p -NPA hydrolysis and CO 2 hydration models. Compared to free CA, immobilization onto the bamboo scaffold increased CA's optimal temperature and pH to approximately 45 °C and 9.0, respectively. Post-immobilization, CA activity demonstrated effective retention (>60 %), with larger scaffold sizes (i.e., 8 mm diameter and 5 mm height) positively impacting this aspect, even surpassing the activity of free CA. Furthermore, immobilized CA exhibited sustained reusability and high stability under thermal treatment and pH fluctuation, retaining >80 % activity even after 5 catalytic cycles. When introduced to microalgae culture, the immobilized CA improved biomass production by ∼16 %, accompanied by enhanced synthesis of essential biomolecules in microalgae. Collectively, the facile and green construction of immobilized CA onto bamboo cellulose block demonstrates great potential for the development of various CA-catalyzed CO 2 conversion and utilization technologies.
研究领域:
生物技术
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环境科学
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材料科学
✓ 已验证
DOI
PubMed
高影响因子
WATER AIR AND SOIL POLLUTION
·
2025
·Vol. 236
·期 4
·1-13
·
IF 3
Liu Lian,Wang Xin,Gao Zixin,Zhan Yue,Yao Mengqin,Bao Jia
Carbon capture, utilization, and storage (CCUS) has emerged as a critical strategy in combating climate change and global warming. Carbonic anhydrase (CA) exhibits exceptional potential as a biocatalyst for CCUS processes due to its remarkable ability to accelerate CO 2 hydration. In this study, CA was immobilized on ZnO nanocarriers of varying morphologies. The optimal immobilization conditions and enzymatic properties of the immobilized CA were investigated, followed by catalytic CO 2 uptake and mineralization experiments. The immobilization process resulted in several improvements: enhanced overall thermal stability, an elevated optimal reaction pH of 8.50 (compared to 8.00 for free CA), retention of 84.98% original activity after 30 days of storage at 4 °C, and preservation of 85.36% initial activity after five recycling cycles. While the affinity between immobilized CA and its substrate increased, a decrease in activity was observed. The Michaelis constant (Km) for immobilized and free CA were determined to be 17.22 mM and 34.92 mM, respectively, with corresponding catalytic efficiency (Kcat/Km) of 20.49 M −1 -S −1 and 148.91 M −1 -S −1 . The catalytic performance of immobilized CA was investigated by carrying out the CO 2 adsorption assay. Notably, the catalytic efficiency of immobilized CA in CO 2 absorption and mineralization under various conditions was significantly enhanced. Graphical
研究领域:
生物化学
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环境科学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
POLYMER
·
2024
·
IF 4.1
Hongyang Deng,Lixian Guo,Keping Chen,Ping Zhang,Yewei Xu,Ningning Song,Chunrong Tian,Jianhua Wang,Shuen Liang
Polyurea (PUA) elastomers have gained increasing attention in both defense and civilian fields due to its remarkable mechanical properties and protective capabilities. However, its high flammability significantly limits its application in many fields. It is challenging to achieve high flame retardant rating and maintain high mechanical properties simultaneously. Synergistic combination of different types of flame retardants is essential for this purpose. Herein we report a highly efficient synergistic combination of 3,3′,4,4′-benzophenonetetracarboxylic dianhydride (BTDA) and ammonium phosphate (APP) for flame retardance of PUA. BTDA contributes to excellent char-forming in solid phase, and APP contributes to radical scavenging in gaseous phase. By incorporating BTDA and APP as additive flame retardants in PUA, the melt dripping in combustion was entirely suppressed, and V-0 rating in UL-94 test was achieved. The heat release rate and the release of smoke and toxic gases were greatly reduced. Meanwhile, the mechanical properties of PUA were maintained very well. The PUA composites with outstanding flame retardancy and mechanical properties may serve as ideal protective materials in a variety of applications.
研究领域:
材料科学
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
Catalysis Science & Technology
·
2024
·
IF 4.4
Kai Cui,Jiaming Yang,Yuli Jing,Junwen Chen,Chen Zhao,Peng Wu,Xiaohong Li
Sustainability issues have led to a gradual market expansion for the 1,4-butanediol (BDO) monomer of the biodegradable plastics to replace conventional plastics. Maleic anhydride (MA) can be derived from the oxidation of bio-based furfural or fructose. Although the hydrogenation of MA can produce a series of products, including succinic anhydride (SA), γ-butyrolactone (GBL), BDO and tetrahydrofuran (THF), the one-pot deep hydrogenation of MA to BDO or THF under mild conditions has been rarely reported in the literature until now. Herein, we report the production of BDO from the one-pot deep hydrogenation of MA over a Cu–0.03Mo/SiO2 catalyst, achieving 100% MA conversion and 88.3% BDO yield. The Cu–0.03Mo/SiO2 catalyst also showed good long-term stability without obvious loss in activity or BDO selectivity during a 160 h time-on-stream test. Doping Mo to Cu/SiO2 catalysts in an optimal amount adjusted the distribution of Cu0/Cu+ species and modulated the interaction of Cu–SiO2 and surface acidity, so that the activation of hydrogen, MA and relevant intermediates can become balanced, in addition to the restriction of side-reactions. This study provides potential for the green synthesis of BDO with non-precious Cu-based catalysts.
研究领域:
化学工程
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可持续发展
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催化科学
✓ 已验证
产品关联
DOI
高影响因子
BIOCONJUGATE CHEMISTRY
·
2025
·
IF 3.9
Jing Wang,Chengxue He,Rui Guo,Li Wen,Jinping Tao,Huimao Zhang,HaiFeng Huang,Hua Zhu,Zhi Yang,Xianteng Yang
Malignant glioma highly expresses carbonic anhydrase IX (CAIX). This study aimed to develop [177Lu]Lu-XYIMSR-01, a small-molecule therapeutic agent CAIX, to assess its potential for treating malignant glioma. [177Lu]Lu-XYIMSR-01 was synthesized by radiolabeling DOTA-XYIMSR-01 with 177Lu. In vitro assays were conducted to evaluate the affinity for U87MG tumor cells. The probe was injected via the tail vein into subcutaneous and orthotopic U87MG models for micro-SPECT/CT imaging. The survival rates of tumor-bearing mice were assessed after [177Lu]Lu-XYIMSR-01 injection by intratumoral in orthotopic models, including untreated controls and those treated with Temozolomide or combination therapy. After purification, the radiochemical yield of [177Lu]Lu-XYIMSR-01 was 86.47 ± 2.42%, with a radiochemical purity (RCP) of 99%. Its cell uptake in U87MG cells was 3.70 ± 0.57 ‰ AD/105 cells, significantly higher than that in HCT116 cells (0.68 ± 0.16 ‰ AD/105 cells, P = 0.001). In the biodistribution study, [177Lu]Lu-XYIMSR-01 uptake in U87MG tumors was 6.19 ± 1.37%ID/g, with a tumor/muscle ratio of 20.14 ± 3.24. In the orthotopic glioma model, local injection combined with Temozolomide significantly improved survival and inhibited tumor growth. The results indicate that [177Lu]Lu-XYIMSR-01 is a promising therapeutic molecular probe for targeting CAIX, and its combination with Temozolomide significantly enhances treatment outcomes for malignant glioma.
研究领域:
肿瘤学
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分子影像学
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核医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 86
·69939-69947
·
IF 3.84
Qin Yan,Hong-Ning Zheng,Chuan Jiang,Kun Li,Shou-Jun Xiao
Polymer brushes of polymethacrylic acid (PMAA) and PMAA-associated polymer blends of PMAA/PNIPAM (poly-N-isopropylacrylamide) were prepared on porous silicon for further investigation of the EDC/NHS (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide) activation mechanisms by infrared spectroscopy. When the fragmentation degree of PMAA blocks in PMAA-associated polymer blends is increased, the production of anhydride wanes from dominant to recessive, whereas a complementary product of the NHS-ester waxes from recessive to dominant. The Thorpe–Ingold effect was applied to explain the formation of anhydride: the gem-dialkyl groups of PMAA next to the carboxylic acids compress the acid side chains close to each other; thus, once the intermediate of O-acylisourea forms, it will be attacked by the intramolecular neighboring acid much faster than any other nucleophiles such as NHS and water, and therefore the six-membered ring of the anhydride will be formed. All acid side chains in PMAA standing next to each other will form an anhydride, primarily due to the Thorpe–Ingold effect, unless they are sterically hindered, whereas only isolated acid side chains form the NHS-ester. The EDC/NHS activation results for four small molecules of dicarboxylic acids in aqueous media, namely, glutaric acid and 2,2-dimethyl glutaric acid, which generate disuccinimidyl ester with high yield, and succinic acid and 2,2-dimethyl succinic acid, which remain intact, can also be explained by the Thorpe–Ingold effect. A clear understanding of the EDC/NHS activation mechanisms of PMAA will take us a step closer for resolving the mechanistic ambiguity of the carbodiimide/additive coupling reactions for amide bond formation.
研究领域:
高分子化学
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生物材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
·
2016
·
IF 1.964
Xiaoyao Fei,Shaoyun Chen,Dai Liu,Chunjie Huang,Yongchun Zhang
Two functionalized SBA-15 [amine-functionalized SBA-15 (AFS) and epoxy-functionalized SBA-15 (GFS)] with different types of functional groups were synthesized by a hydrothermal process and post functionalized with 3-aminopropyltriethoxysilane (APTES) and 3-glycidyloxypropyltrimethoxysilane (GPTMS), respectively. They were used for the immobilization of carbonic anhydrase (CA). The physicochemical properties of the functionalized SBA-15 were characterized by X-ray powder diffraction (XRD), N 2 adsorption–desorption, 13 C, 29 Si solid-state nuclear magnetic resonance (NMR) spectroscopy, and scanning electron microscopy (SEM). Before and after CA was immobilized on AFS and GFS, the effects of temperature and pH value on the enzyme activity , storage stability, and reusability were investigated using para -nitrophenyl acetate ( p -NPA) assay. CA/GFS showed a better performance with respect to storage stability and reusability than CA/AFS. Moreover, the amount of CaCO 3 precipitated over CA/AFS was less than that precipitated over CA/GFS, which was almost equal to that precipitated over the free CA. The results indicate that the epoxy group is a more suitable functional group for covalent bonding with CA than the amino group, and GFS is a promising support for CA immobilization.
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Green Chemical Engineering
·
2022
·
IF 0
Yumei Liu,Shengxin Chen,Ran An,Xingsheng Wang,Ruixia Liu
The sustainable synthesis of dimethyl maleate via diesterification through the utilization of ionic liquid (IL) is of great importance. However, the relationship between the ILs nature and the reactivity of diesterification is still unclear. Herein, a series of ILs with different structures were selected for the comprehensive investigation of diesterification. The acidity (H 0 ) and Kamlet-Taft solvent parameters (hydrogen bond donor ability (α), hydrogen bond acceptor ability (β), and polarizability (π∗)) of ILs were measured by UV–Visible spectroscopy, and the effects of them on the diesterification of maleic anhydride were also studied in detail. The results indicated that not only H 0 of the IL-based catalysis system, but also its α, β, and π∗ influenced the reaction activity of diesterification. Furthermore, a quantifiable correlation was fitted between the natural logarithm of the rate constant and multiple parameters of ILs, indicating that the diesterification rate had a positive correlation with the H 0 , α, and π∗, and inverse correlation with the β of the IL. A plausible synergetic reaction mechanism for the excellent performance of [(HSO 3 )PMim][HSO 4 ] has been proposed. Overall, this work thoroughly explored the relationship between the nature of ILs on diesterification in-depth, which will reveal the nature of diesterification in detail.
研究领域:
化学工程
·
催化化学
✓ 已验证
DOI
Small
·
2021
·Vol. 17
·期 50
·2103336
·
IF 13.281
Xuping Liu,Jihuai Wu,Chunyan Wang,Yuqian Yang,Deng Wang,Guodong Li,Yitian Du,Yuan Xu,Lei Zhang,Tingting Zhang,Lan Zhang
The carrier non-radiative recombination and instability of device caused by the inherent defects are main factors limiting development of perovskite solar cells (PSCs). During the fabrication process of a PSC device, perovskite films often produce Pb 0 and I 0 defects. This paper reports a strategy for synergistic optimization of perovskite films by defects passivation and surface modification. The doping of phthalide (PT) in the Pb-rich (CH(NH 2 ) 2 ) 1−x (CH 3 NH 3 ) x PbI 3 film can passivate lead cation defects, and the modification of 1-iodooctadecane (1-IO) can reduce halogen anion defects and improve stability of PSCs owing to its hydrophobicity. The PT and 1-IO optimized device achieves a power conversion efficiency (PCE) of 22.27%. The optimized PSCs remain 93.2% of the initial PCE when placed in air environment (relative humidity of 10%, 25 °C) more than 70 days. The PT and 1-IO synergistic optimization provides a novel strategy for improving the performance and stability of PSCs.
研究领域:
材料科学
·
能源技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）